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Eun Mi Choi - One of the best experts on this subject based on the ideXlab platform.
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glabridin alleviates the toxic effects of methylglyoxal on osteoblastic MC3T3 e1 cells by increasing expression of the glyoxalase system and nrf2 ho 1 signaling and protecting mitochondrial function
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Eun Mi Choi, Soo Min Hong, So Yong Park, Suk ChonAbstract:Methylglyoxal (MG) contributes to the pathogenesis of age- and diabetes-associated complications. The present study investigated the effects of glabridin on MG-induced cytotoxicity in MC3T3-E1 osteoblastic cells. MC3T3-E1 cells were treated with glabridin in the presence of MG, and markers of mitochondrial function and oxidative damage were examined. Pretreatment of MC3T3-E1 osteoblastic cells with glabridin prevented MG-induced cell death, the production of intracellular reactive oxygen species and mitochondrial superoxides, cardiolipin peroxidation, and the production of inflammatory cytokines. The soluble form of receptor for advanced glycation end products (sRAGEs)/RAGE ratio increased upon MG treatment, but less so after pretreatment with glabridin, which also increased the level of reduced glutathione and the activities of glyoxalase I and heme oxygenase-1, all of which were reduced by MG. In addition, glabridin elevated the level of nuclear factor erythroid 2-related factor 2. These findings sugges...
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luteolin protects osteoblastic MC3T3 e1 cells from antimycin a induced cytotoxicity through the improved mitochondrial function and activation of pi3k akt creb
Toxicology in Vitro, 2011Co-Authors: Eun Mi ChoiAbstract:Luteolin is a flavonoid found in many herbal extracts including celery, green pepper, parsley, perilla leaf and seeds, and chamomile. Antimycin A (AMA) is an inhibitor of the mitochondrial electron transport chain. In the present study, the protective effect of luteolin on AMA-induced cell damage was investigated in osteoblastic MC3T3-E1 cells. Luteolin significantly increased the viability of MC3T3-E1 cells in the presence of AMA and the effect of luteolin in increasing cell viability was completely prevented by the presence of LY294002, Akt inhibitor, or auranofin, suggesting that the effect of luteolin might be partly mediated from PI3K, Akt, and thioredoxin reductase. Pre-treatment with luteolin prior to AMA exposure significantly prevented mitochondrial membrane potential dissipation, ATP loss, inactivation of complex I and IV, ROS production, inactivation of thioredoxin reductase, intracellular calcium elevation, and cytochrome c release induced by AMA. Moreover, luteolin increased activities of PI3K (phosphoinositide 3-kinase) and Akt (protein kinase B), and CREB (cAMP-response element-binding protein) phosphorylation inhibited by AMA treatment. Collectively, these results suggest that luteolin protects MC3T3-E1 cells from AMA-induced cell damage through the improved mitochondrial function and activation of PI3K/Akt/CREB.
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hesperetin attenuates the highly reducing sugar triggered inhibition of osteoblast differentiation
Cell Biology and Toxicology, 2008Co-Authors: Eun Mi Choi, Young Ho KimAbstract:Diabetic bone disease is associated with increased oxidative damage and 2-deoxy-d-ribose (dRib) is used to induce oxidative damage similar to that observed in diabetics. To determine if hesperetin (3′,5,7-trihydroxy-4-methoxyflavanone) could influence osteoblast dysfunction induced by dRib, osteoblastic MC3T3-E1 cells were treated with dRib and hesperetin. Then, markers of osteoblast function and oxidative damage were examined. Hesperetin (10−7–10−5 M) caused a significant elevation of alkaline phosphatase (ALP) activity, collagen content, and total antioxidant potential of MC3T3-E1 cells in the presence of 20 mM dRib (p < 0.05). Moreover, hesperetin (10−7 M) decreased cellular protein carbonyl (PCO), advanced oxidation protein products (AOPP), and malondialdehyde (MDA) contents of osteoblastic MC3T3-E1 cells in the presence of 20 mM dRib. These results demonstrate that hesperetin attenuates dRib-induced damage, suggesting that hesperetin may be a useful dietary supplement for minimizing oxidative injury in diabetes related bone diseases.
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effect of 6 gingerol a pungent ingredient of ginger on osteoblast response to extracellular reducing sugar
Food Science and Biotechnology, 2007Co-Authors: Eun Mi ChoiAbstract:Diabetes is marked by high glucose levels and is associated with decreased bone mass and increased fracture rates. To determine if [6]-gingerol could influence osteoblast dysfunction induced by 2-deoxy-D-ribose (dRib), osteoblastic MC3T3- E1 cells was treated with dRib and [6]-gingerol and markers of osteoblast function and oxidized protein were examined. [6]- Gingerol (10 -7 M) significantly increased the growth of MC3T3-E1 cells in the presence of 30 mM dRib (p -7 M) caused a significant elevation of alkaline phosphatase (ALP) activity, collagen content, and osteocalcin secretion in the cells. We then examined the effect of [6]-gingerol on the production of osteoprotegerin and protein carbonyl in osteoblasts. Treatment with [6]-gingerol (10 -9 and 10 -7 M) increased osteoprotegerin secretion in osteoblastic cells. Moreover, [6]-gingerol (10 -9 and 10 -7 M) decreased protein carbonyl contents of osteoblastic MC3T3-E1 cells in the presence of 30 mM dRib. Taken together, these results demonstrate that [6]-gingerol inhibits dRib-induced damage and may be useful in the treatment of diabetes related bone diseases.
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stimulatory effects of extract prepared from the bark of cinnamomum cassia blume on the function of osteoblastic MC3T3 e1 cells
Phytotherapy Research, 2006Co-Authors: Kyunghee Lee, Eun Mi ChoiAbstract:The ethanol extract from the bark of Cinnamomum cassia Blume (CCE) was tested for estrogenic activity. CCE (4-60 microg/mL) significantly induced the growth of MCF-7 cells, an ER-positive human breast cancer cell line, over that of untreated control cells (p < 0.05). In the ER competitive binding assay, CCE showed higher affinity with ERbeta compared with ERalpha. To investigate the bioactivities of CCE, which act on bone metabolism, the effects of CCE on the function of osteoblastic MC3T3-E1 cells and the production of local factors in osteoblasts were studied. CCE (4-60 microg/mL) dose-dependently increased the survival of MC3T3-E1 cells. In addition, CCE (10 and 50 microg/mL) increased alkaline phosphatase (ALP) activity, collagen synthesis and osteocalcin secretion in MC3T3-E1 cells. Treatment with CCE (10 and 50 microg/mL) prevented apoptosis induced by TNF-alpha (10(-10) m) in osteoblastic cells. In the presence of TNF-alpha, culture with CCE (10-100 microg/mL) for 48 h inhibited the production of IL-6 and nitric oxide in osteoblastic MC3T3-E1 cells. These results suggest that Cinnamomum cassia has a direct stimulatory effect on bone formation in vitro and may contribute to the prevention of osteoporosis and inflammatory bone diseases.
Hong Wang - One of the best experts on this subject based on the ideXlab platform.
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agrimonia pilosa polysaccharide and its sulfate derives facilitate cell proliferation and osteogenic differentiation of MC3T3 e1 cells by targeting mir 107
International Journal of Biological Macromolecules, 2020Co-Authors: Wei Huang, Shengyang Jin, Wenbo Yang, Shuo Tian, Chunqing Meng, Huan Deng, Chong Wang, Hong WangAbstract:The present study showed that low level of miR-107 in femoral head tissues from rats suffering steroid-induced osteonecrosis of the femoral head (SANFH) was associated with high degree of osteonecrosis and apoptosis. In vitro assay, pretreatment with an Agrimonia pilos polysaccharide (APP-AW) and its sulphated derivatives (S2) at 50 μg/ml for 48 h or overexpressing miR-107 were able to prevent cytotoxicity induced by 1 μM dexamethasone (DEX) in MC3T3-E1 cells via inhibition of apoptosis. Meanwhile, the decreased ALP activity, collagen content, BMP2, Runx2, OSX and OCN protein expression in DEX-treated MC3T3-E1 cells were rescued by the addition of APP-AW and S2, or miR-107 transfection. Moreover, DEX-induced increase of Bax, cytochrome c and caspase-3, as well as decrease of Bcl-2, Wnt3, β-catenin and c-Myc protein expression in MC3T3-E1 cells were also reversed. These findings suggest that APP-AW and S2 promote cell proliferation and osteogenic differentiation by enhancing miR-107 during the development of SANFH.
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protective effect of agrimonia pilosa polysaccharides on dexamethasone treated MC3T3 e1 cells via wnt β catenin pathway
Journal of Cellular and Molecular Medicine, 2020Co-Authors: Wei Huang, Shengyang Jin, Wenbo Yang, Shuo Tian, Chunqing Meng, Huan Deng, Hong WangAbstract:A water-soluble polysaccharide (APP-AW) was isolated from Agrimonia pilosa and prepared to three sulphated derivatives (S1, S2 and S3). The results showed that pre-treatment with APP-AW, S1, S2 and S3 each at the concentration of 50 μg/mL for 48 hours was able to prevent cytotoxicity induced by 1 μmol/L dexamethasone (Dex) in MC3T3-E1 cells via inhibition of apoptosis, which is in line with the findings in flow cytometry analysis. Meanwhile, the decreased ALP activity, collagen content, mineralization, BMP2, Runx2, OSX and OCN protein expression in DEX-treated MC3T3-E1 cells were reversed by the addition of APP-AW, S1, S2 and S3. Moreover, APP-AW, S1, S2 and S3 rescued DEX-induced increase of Bax, cytochrome c and caspase-3 and decrease of Bcl-2, Wnt3, β-catenin and c-Myc protein expression in MC3T3-E1 cells. Our findings suggest that pre-treatment with APP-AW, S1, S2 and S3 could significantly protect MC3T3-E1 cells against Dex-induced cell injury via inhibiting apoptosis and activating Wnt/β-Catenin signalling pathway, thus application of these polysaccharides may be a promising alternative strategy for steroid-induced avascular necrosis of the femoral head (SANFH) therapy.
Watt-smith, Stephen R - One of the best experts on this subject based on the ideXlab platform.
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Enhancing Distraction Osteogenesis With Carbon Fiber Reinforced Polyether Ether Ketone Bone Pins and a Three-Dimensional Printed Transfer Device to Permit Artifact-Free Three-Dimensional Magnetic Resonance Imaging.
'Organisation for Economic Co-Operation and Development (OECD)', 2021Co-Authors: Eley, Karen Ann, Moazen Mehran, Delso Gaspar, Javidan Maryam, Kaggie Joshua, Kalaskar, Deepak M, Knowles, Jonathan C, Owji Nazanin, Watt-smith, Stephen RAbstract:Abstract: Objectives: To: (1) design an artefact-free 3D-printed MR-safe temporary transfer device, (2) engineer bone-pins from Carbon Fibre Reinforced (CFR-PEEK), (3) evaluate the imaging artefacts of CFR-PEEK, and (4) confirm the osteointegration potential of CFR-PEEK, thus enhancing 3Dplanning of bony advancements in hemifacial microsomia using sequential MRI. Study Design: Engineered CRF-PEEK bone pins and a 3D printed ex-fix device were implanted into a sheep head and imaged with MRI and CT. The osseointegration and bony compatibility potential of CFR-PEEK was assessed with Scanning Electron Microscopy (SEM) images of MC3T3 preosteoblast cells on the surface of the material. Results: The CFR-PEEK pins resulted in a signal void equivalent to the dimension of the pin, with no adjacent areas of MR-signal loss or CT artefact. MCT3 cells adhered and proliferated on the surface of the discs by forming a monolayer of cells, confirming compatibility and osseointegration potential. Conclusion: A 3D printed transfer device could be utilised temporarily during MRI to permit artefact-free 3D planning. CFR-PEEK pins eliminate imaging artefact permitting sequential MRI examination. In combination, this has the potential to enhance distraction osteogenesis, by permitting accurate three-dimensional planning without ionising radiation.Newlife Charity for Disabled Children Academy of Medical Science
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Enhancing Distraction Osteogenesis With CFR-PEEK Bone Pins and a 3D Printed Transfer Device to Permit Artefact-free Three-dimensional Magnetic Resonance Imaging.
'Organisation for Economic Co-Operation and Development (OECD)', 2020Co-Authors: Eley, Karen Ann, Moazen Mehran, Delso Gaspar, Javidan Maryam, Kaggie Joshua, Kalaskar, Deepak M, Knowles, Jonathan C, Owji Nazanin, Watt-smith, Stephen RAbstract:Abstract: Objectives: To: (1) design an artefact-free 3D-printed MR-safe temporary transfer device, (2) engineer bone-pins from Carbon Fibre Reinforced (CFR-PEEK), (3) evaluate the imaging artefacts of CFR-PEEK, and (4) confirm the osteointegration potential of CFR-PEEK, thus enhancing 3Dplanning of bony advancements in hemifacial microsomia using sequential MRI. Study Design: Engineered CRF-PEEK bone pins and a 3D printed ex-fix device were implanted into a sheep head and imaged with MRI and CT. The osseointegration and bony compatibility potential of CFR-PEEK was assessed with Scanning Electron Microscopy (SEM) images of MC3T3 preosteoblast cells on the surface of the material. Results: The CFR-PEEK pins resulted in a signal void equivalent to the dimension of the pin, with no adjacent areas of MR-signal loss or CT artefact. MCT3 cells adhered and proliferated on the surface of the discs by forming a monolayer of cells, confirming compatibility and osseointegration potential. Conclusion: A 3D printed transfer device could be utilised temporarily during MRI to permit artefact-free 3D planning. CFR-PEEK pins eliminate imaging artefact permitting sequential MRI examination. In combination, this has the potential to enhance distraction osteogenesis, by permitting accurate three-dimensional planning without ionising radiation.Newlife Charity for Disabled Children Academy of Medical Science
Wei Huang - One of the best experts on this subject based on the ideXlab platform.
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agrimonia pilosa polysaccharide and its sulfate derives facilitate cell proliferation and osteogenic differentiation of MC3T3 e1 cells by targeting mir 107
International Journal of Biological Macromolecules, 2020Co-Authors: Wei Huang, Shengyang Jin, Wenbo Yang, Shuo Tian, Chunqing Meng, Huan Deng, Chong Wang, Hong WangAbstract:The present study showed that low level of miR-107 in femoral head tissues from rats suffering steroid-induced osteonecrosis of the femoral head (SANFH) was associated with high degree of osteonecrosis and apoptosis. In vitro assay, pretreatment with an Agrimonia pilos polysaccharide (APP-AW) and its sulphated derivatives (S2) at 50 μg/ml for 48 h or overexpressing miR-107 were able to prevent cytotoxicity induced by 1 μM dexamethasone (DEX) in MC3T3-E1 cells via inhibition of apoptosis. Meanwhile, the decreased ALP activity, collagen content, BMP2, Runx2, OSX and OCN protein expression in DEX-treated MC3T3-E1 cells were rescued by the addition of APP-AW and S2, or miR-107 transfection. Moreover, DEX-induced increase of Bax, cytochrome c and caspase-3, as well as decrease of Bcl-2, Wnt3, β-catenin and c-Myc protein expression in MC3T3-E1 cells were also reversed. These findings suggest that APP-AW and S2 promote cell proliferation and osteogenic differentiation by enhancing miR-107 during the development of SANFH.
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protective effect of agrimonia pilosa polysaccharides on dexamethasone treated MC3T3 e1 cells via wnt β catenin pathway
Journal of Cellular and Molecular Medicine, 2020Co-Authors: Wei Huang, Shengyang Jin, Wenbo Yang, Shuo Tian, Chunqing Meng, Huan Deng, Hong WangAbstract:A water-soluble polysaccharide (APP-AW) was isolated from Agrimonia pilosa and prepared to three sulphated derivatives (S1, S2 and S3). The results showed that pre-treatment with APP-AW, S1, S2 and S3 each at the concentration of 50 μg/mL for 48 hours was able to prevent cytotoxicity induced by 1 μmol/L dexamethasone (Dex) in MC3T3-E1 cells via inhibition of apoptosis, which is in line with the findings in flow cytometry analysis. Meanwhile, the decreased ALP activity, collagen content, mineralization, BMP2, Runx2, OSX and OCN protein expression in DEX-treated MC3T3-E1 cells were reversed by the addition of APP-AW, S1, S2 and S3. Moreover, APP-AW, S1, S2 and S3 rescued DEX-induced increase of Bax, cytochrome c and caspase-3 and decrease of Bcl-2, Wnt3, β-catenin and c-Myc protein expression in MC3T3-E1 cells. Our findings suggest that pre-treatment with APP-AW, S1, S2 and S3 could significantly protect MC3T3-E1 cells against Dex-induced cell injury via inhibiting apoptosis and activating Wnt/β-Catenin signalling pathway, thus application of these polysaccharides may be a promising alternative strategy for steroid-induced avascular necrosis of the femoral head (SANFH) therapy.
Guicai Sun - One of the best experts on this subject based on the ideXlab platform.
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z guggulsterone attenuates glucocorticoid induced osteoporosis through activation of nrf2 ho 1 signaling
Life Sciences, 2019Co-Authors: Jian Guan, Shuilin Chen, Guicai SunAbstract:Abstract Aims The present study aims to investigate the protective effect and underlying mechanism of Z-Guggulsterone (Z-GS), an active component from myrrh, on glucocorticoid-induced osteoporosis (GIO). Main methods GIO rats were used to simulate osteoporosis in vivo while MC3T3-E1 cells were induced to osteoblast differentiation and treated with dexamethasone to simulate osteoporosis in vitro. The rats and cells were treated with Z-GS according to the protocol. The bone mineral density, biomechanical parameters and microstructure of GIO rats were measured with appropriate devices. Cell viability of MC3T3-E1 cells were analyzed via CCK-8 assay. Bone turnover markers and oxidative stress markers were detected by ELISA, and the expressions of Nrf2 and HO-1 were assessed by western blot. siRNA-Nrf2 and siRNA-HO-1 were transfected in MC3T3-E1 cells to knockdown the expressions of Nrf2 and HO-1. Key findings Z-GS significantly increased the body weights and bone mineral density, ameliorated the femoral biomechanical parameters and microstructure of GIO rats. Z-GS treatment also reversed DXM-induced changes of bone turnover markers and oxidative stress in rats and MC3T3-E1 cells. The expressions of Nrf2 and HO-1 were inhibited in the model group and treatment with Z-GS could markedly increase their expressions. Nrf2 or HO-1 knockdown observably abrogated the beneficial role of Z-GS on cells. Significance Our results demonstrated that Z-GS exerted bone protective and antioxidant stress properties through activation of Nrf2/HO-1 signaling in GIO models in vivo and in vitro. Therefore, Z-GS could be considered as a promising candidate for the treatment of GIO.