The Experts below are selected from a list of 25290 Experts worldwide ranked by ideXlab platform
S. Yousef Ebrahimipour - One of the best experts on this subject based on the ideXlab platform.
-
Green synthesis of starch-mediated CuO nanoparticles: preparation, characterization, antimicrobial activities and in vitro MTT assay against MCF-7 Cell Line
Rendiconti Lincei, 2016Co-Authors: Hossein Alishah, Shahram Pourseyedi, Saeed Esmaili Mahani, S. Yousef Ebrahimipour, Nahid RafieiAbstract:The main goal of this study was the synthesis of copper oxide nanoparticles with a green method using Starch extracted from Solanum tuberosum. The synthesized copper oxide nanoparticles were characterized by different techniques, including X-ray diffraction (XRD), UV–Vis spectrophotometry, and scanning electron microscopy (SEM). All the analyses exposed that the nanoparticles were highly stable and spherical with mean size about 54 nm. The potential antibacterial activity of the copper oxide nanoparticles synthesized in the eco-friendly approach was tested against some standard strains of human pathogens (Gram-positive and -negative bacteria). Efficient antibacterial activities against Bacillus cereus 9634, Shigella sonnei, Staphylococcus epidermidis 1435, Enterococcus 1237, Pseudomonas aeruginosa PAO1 27853 and Escherichia coli 25922 and no significant antibacterial activity against Staphylococcus aureus 2592 were observed. Moreover, MTT assay demonstrated the anticancer activity ofthat the synthesized CuO nanoparticles against MCF-7 Cell Line.
-
Extract-mediated synthesis of Ag@AgCl nanoparticles using Conium maculatum seeds: characterization, antibacterial activity and cytotoxicity effect against MCF-7 Cell Line
RSC Advances, 2016Co-Authors: Hossein Alishah, Shahram Pourseyedi, Saeed Esmaili Mahani, S. Yousef EbrahimipourAbstract:In this study, silver/silver chloride nanoparticles (Ag@AgCl-NPs) were successfully synthesized using silver nitrate (AgNO3) via an eco-friendly and simple green approach using the seed extract of Conium maculatum (SECM) at room temperature. The biologically synthesized Ag@AgCl-NPs were characterized by UV-vis spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). Electron microscopic imaging showed that the size of the Ag@AgCl-NPs was about 20 nm, with the particles spherical in shape. Moreover, the crystalLine structure of the synthesized nanoparticles was confirmed using the XRD pattern. The potential antibacterial activity of the NPs against standard strains of human pathogens (Gram-positive and negative bacteria) and in vitro anticancer activity against the MCF-7 Cell Line were investigated. The results demonstrated the ability of the synthesized nanoparticle to kill the cancer Cells and inhibit the growth of the human pathogenic and standard strains.
André Arigony Souto - One of the best experts on this subject based on the ideXlab platform.
-
Antitumor activity of resveratrol is independent of Cu(II) complex formation in MCF-7 Cell Line.
Bioorganic & medicinal chemistry letters, 2017Co-Authors: Priscylla Andrade Volkart, Rodrigo Benedetti Gassen, Bettina Mühlen Nogueira, Bárbara Nery Porto, José Eduardo Vargas, André Arigony SoutoAbstract:Resveratrol (Rsv) is widely reported to possess anticarcinogenic properties in a plethora of Cellular and animal models having limited toxicity toward normal Cells. In the molecular level, Rsv can act as a suppressive agent for several impaired signaling pathways on cancer Cells. However, Fukuhara and Miyata have shown a non-proteic reaction of Rsv, which can act as a prooxidant agent in the presence of copper (Cu), causing Cellular oxidative stress accompanied of DNA damage. After this discovery, the complex Rsv-Cu was broadly explored as an antitumor mechanism in multiples tumor Cell Lines. The aim of the study is to explore the anticarcinogenic behavior of resveratrol-Cu(II) complex in MCF-7 Cell Line. Selectivity of Rsv binding to Cu ions was analyzed by HPLC and UV-VIS. The Cells were enriched with concentrations of 10 and 50µM CuSO4 solution and treated with 25µM of Rsv. Copper uptake after enrichment of Cells, as its intraCellular distribution in MCF-7 Line, was scanned by ICP-MS and TEM-EDS. Cell death and intraCellular ROS production were determined by flow cytometry. Different from the extraCellular model, no relationship of synergy between Rsv-Cu(II) and reactive oxidative species (ROS) production was detected in vitro. ICP-MS revealed intraCellular copper accumulation to both chosen concentrations (0.33±0.09 and 1.18±0.13ppb) but there is no promotion of Cell death by Rsv-Cu(II) complex. In addition, significant attenuation of ROS production was detected when Cells were exposed to CuSO4 after Rsv treatment, falling from 7.54% of ROS production when treated only with Rsv to 3.07 and 2.72% with CuSO4. Based on these findings antitumor activity of resveratrol when in copper ions presence, is not mediated by Rsv-Cu complex formation in MCF-7 human Cell Line, suggesting that the antitumoral reaction is dependent of a cancer Cellular model.
Abeer E. Mahmoud - One of the best experts on this subject based on the ideXlab platform.
-
synthesis and in vitro cytotoxic activity of novel pyrazolo 3 4 d pyrimidines and related pyrazole hydrazones toward breast adenocarcinoma mcf 7 Cell Line
Bioorganic & Medicinal Chemistry, 2011Co-Authors: Ghaneya S Hassan, Hanan H Kadry, Sahar M Abouseri, Abeer E. MahmoudAbstract:Abstract New series of pyrazolo[3,4- d ]pyrimidines ( 7a–e and 13a–d ) and pyrazole hydrazones 17a–d were synthesized and evaluated for their antiproliferative activity against human breast adenocarcinoma MCF-7 Cell Line. Most of the tested compounds exploited potent to moderate growth inhibitory activity, in particular compound 7e exhibited superior potency to the reference drug cisplatin (IC 50 = 7.60 and 13.29 μM, respectively). The antitumor activity of the new compounds was accompanied by significant increase in the activity of superoxide dismutase with concomitant decrease in the activities of catalase and glutathione peroxidase and reduced glutathione level. Accordingly, the overproduction of hydrogen peroxide, nitric oxide and other free radicals allowed reactive oxygen species (ROS)-mediated tumor Cells death, as monitored by reduction in the synthesis of protein and nucleic acids.
Yetrib Hathout - One of the best experts on this subject based on the ideXlab platform.
-
Highly Altered Protein Expression Profile in the Adriamycin Resistant MCF-7 Cell Line
Journal of proteome research, 2004Co-Authors: Marion L. Gehrmann, Catherine Fenselau, Yetrib HathoutAbstract:The protein expression pattern in the cytosol fraction of the adriamycin resistant MCF-7 Cell Line (MCF-7/ADR) was compared to that of the parental MCF-7 Cell Line using two-dimensional gel electrophoresis and mass spectrometry. Twenty proteins with altered abundances were identified and studied in MCF-7/ADR. Both up regulation and down regulation are characterized. The most striking differences were found for proteins that were uniquely expressed in this Cell Line and not detectable in the parental MCF-7 Cell Line. These proteins include annexin I, the neuronal ubiquitin carboxyl hydrolase isoenzyme L-1 (also known as PGP9.5), glutathione-S-transferase pi class, nicotinamide N-methyltransferase, and interleukin-18 precursor. On the other hand, catechol-O-methyltransferase was expressed in the parental Cell Line, but was not detected in the adriamycin resistant Cell Line. This protein expression pattern was unique to MCF-7/ADR and not observed in MCF-7 Cell Lines selected for resistant to etoposide, mitoxantrone or melphalan.
Khalid S Almaary - One of the best experts on this subject based on the ideXlab platform.
-
A novel biogenic Allium cepa leaf mediated silver nanoparticles for antimicrobial, antioxidant, and anticancer effects on MCF-7 Cell Line.
Environmental research, 2021Co-Authors: M Mani, Mohammad K Okla, S Selvaraj, A Ram Kumar, S Kumaresan, Azhaguchamy Muthukumaran, K Kaviyarasu, Mohamed A El-tayeb, Yahya B Elbadawi, Khalid S AlmaaryAbstract:In the present study, Allium cepa leaf extract was utilized to reduce the silver nitrate into the nanoscale range of silver ions (Ag NPs). The biosynthesized Ag NPs were extensively characterized by X-ray diffraction analysis (XRD), Dynamic light scattering analysis (DLS), UV-Visible spectroscopy (UV-vis), Transmission electron microscopy (TEM), Energy dispersive X-ray analysis (EDX) and Fourier transform infrared spectroscopy (FTIR). The antioxidant activity of synthesized Ag NPs was verified by DPPH assay. From the results obtained from XRD and DLS studies, the size of Ag NPs was determined to be around 54.3 nm. The measured zeta potential value of -19.1 mV confirms the exCellent stability of biosynthesized Ag NPs. TEM analyses reveal that the biosynthesized Ag NPs have a spherical structure of 13 nm in size. The presence of various functional groups was confirmed through FTIR studies and EDAX verifies the weight percentage of silver content in biosynthesized nanoparticles to be 30.33%. In the present study, anti-cancer activity was carried out by using breast cancer Cell Line MCF-7. Further, silver nanoparticles exhibited antimicrobial effectiveness against gram-positive Bacillus cereus and gram-negative Escherichia coli. The MTT assay also showed better cytotoxic activity against the MCF- 7 Cell Line.