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Min Kyoung Park - One of the best experts on this subject based on the ideXlab platform.
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annona muricata leaf extract triggered intrinsic apoptotic pathway to attenuate cancerous features of triple negative breast cancer mda mb 231 Cells
Evidence-based Complementary and Alternative Medicine, 2018Co-Authors: Kwangho Song, Sait Byul Park, Hyeri Jang, Min Kyoung ParkAbstract:Annona muricata L., known as graviola, is an evergreen plant of the tropical regions and is a rich source of natural products. Graviola has various biological activities, and it is best known for its anticancer activity. This study aimed to investigate the effects of crude graviola extract in vitro on breast cancer Cells; in particular, we aimed to identify an agent against triple negative breast cancer (TNBC). We used the TNBC MDA-MB-231 Cell Line as the experimental model and the ER(+) non-TNBC MCF-7 breast cancer Cell Line as the control. We identified annonaceous acetogenins, including annonacin isomers, characteristic to this plant by using liquid chromatography tandem mass spectrometry (LC/MS/MS). We observed a significant decrease in the Cell viability in both Cell Lines within 48 h, whereas impaired Cell motility and invasiveness were observed only in the MDA-MB-231 Cell Line. While the MCF-7 Cells showed an ER-dependent mechanism of apoptosis, the apoptosis of MDA-MB-231 Cells was governed by an intrinsic apoptotic pathway triggered by graviola leaf extract (GLE).
M A Navarro - One of the best experts on this subject based on the ideXlab platform.
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Secretion and dual regulation between epidermal growth factor and transforming growth factor-beta1 in MDA-MB-231 Cell Line in 42-hour-long cultures.
Cancer letters, 1999Co-Authors: P A Martínez-carpio, M E Fernández-montolí, J M Ramon, P Rosel, M A NavarroAbstract:MDA-MB-231 is a breast cancer Cell Line which possesses large quantities of epidermal growth factor (EGF) receptors and specific high-affinity transforming growth factor-beta1 (TGF-beta1) receptors. We have established that these Cells secrete constitutively measurable levels of EGF and TGF-beta1 in conditioned medium. The constitutive secretion of EGF decreased over time in culture (42 h), while the constitutive secretion of TGF-beta1 remained constant. TGF-beta1 secretion in EGF-treated Cells was lower than in controls (P < 0.0001), but EGF concentrations were not modified after TGF-beta1 supplement. We postulate that in MDA-MB-231 Cell Line there is a dual regulation between both growth factors.
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Secretion and dual regulation between epidermal growth factor and transforming growth factor-β1 in MDA-MB-231 Cell Line in 42-hour-long cultures
Cancer Letters, 1999Co-Authors: Pere A Martı́nez-carpio, P Rosel, María E. Fernández-montolí, Josep Ma Ramon, M A NavarroAbstract:MDA-MB-231 is a breast cancer Cell Line which possesses large quantities of epidermal growth factor (EGF) receptors and specific high-affinity transforming growth factor-β1 (TGF-β1) receptors. We have established that these Cells secrete constitutively measurable levels of EGF and TGF-β1 in conditioned medium. The constitutive secretion of EGF decreased over time in culture (42 h), while the constitutive secretion of TGF-β1 remained constant. TGF-β1 secretion in EGF-treated Cells was lower than in controls (P
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Growth of MDA-MB-231 Cell Line: different effects of TGF-beta(1), EGF and estradiol depending on the length of exposure.
Cell Biology International, 1998Co-Authors: Pere A Martı́nez-carpio, P Rosel, María E. Fernández-montolí, Josep Ma Ramon, M A NavarroAbstract:Abstract The human Cell Line MDA-MB-231 is a prototype for the study of hormone-independent breast cancer. Modification of Cell growth behaviour has been observed after treating these Cells with growth factors. EGF is a typical stimulatory growth factor for many Cell types, whereas transforming growth factor β1(TGF-β1) acts with inhibitory character. Here we observed Cell growth inhibition after EGF as well as after TGF-β1treatments. Nevertheless, in the 42-h experiments, EGF-treated cultures grew before (18 hours) respect to the TGF-β1and E2-treated cultures (24 h), and in the 11-day experiments, EGF-treated cultures started growing (7 days) after TGF-β1-treated cultures (5 days). Estradiol inhibited the proliferation of these Cells only after several days of treatment.
Kwangho Song - One of the best experts on this subject based on the ideXlab platform.
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annona muricata leaf extract triggered intrinsic apoptotic pathway to attenuate cancerous features of triple negative breast cancer mda mb 231 Cells
Evidence-based Complementary and Alternative Medicine, 2018Co-Authors: Kwangho Song, Sait Byul Park, Hyeri Jang, Min Kyoung ParkAbstract:Annona muricata L., known as graviola, is an evergreen plant of the tropical regions and is a rich source of natural products. Graviola has various biological activities, and it is best known for its anticancer activity. This study aimed to investigate the effects of crude graviola extract in vitro on breast cancer Cells; in particular, we aimed to identify an agent against triple negative breast cancer (TNBC). We used the TNBC MDA-MB-231 Cell Line as the experimental model and the ER(+) non-TNBC MCF-7 breast cancer Cell Line as the control. We identified annonaceous acetogenins, including annonacin isomers, characteristic to this plant by using liquid chromatography tandem mass spectrometry (LC/MS/MS). We observed a significant decrease in the Cell viability in both Cell Lines within 48 h, whereas impaired Cell motility and invasiveness were observed only in the MDA-MB-231 Cell Line. While the MCF-7 Cells showed an ER-dependent mechanism of apoptosis, the apoptosis of MDA-MB-231 Cells was governed by an intrinsic apoptotic pathway triggered by graviola leaf extract (GLE).
Xin-wei Zhang - One of the best experts on this subject based on the ideXlab platform.
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Establishment of a bioluminescent MDA-MB-231 Cell Line for human triple-negative breast cancer research.
Oncology Reports, 2012Co-Authors: Ke Wang, Xin-wei Zhang, Jianjun HeAbstract:The aim of this study was to establish a bioluminescent MDA-MB-231 Cell Line stably expressing luciferase and green fluorescent protein for the generation of a xenografted model of human triple-negative breast cancer (TNBC) in nude mice. Lentivirus vectors carrying eGFP, firefly luc2 and neo fusion genes were used to transduce the MDA-MB-231 human TNBC Cells in vitro. After 8 weeks of G418 selection, eGFP and luc2 expression was determined using a fluorescence microscope and a Xenogen IVIS200 bioluminescent imaging system, respectively. The MTT, transwell invasion and wound healing assays were performed to confirm whether Cellular proliferation, invasion and migration were altered by lentiviral infection. Cells were orthotopically implanted into female BALB/c nude mice to test the sensitivity and stability of reporter gene expression. Growth of the tumors was monitored with the in vivo imaging system once a week until they were large enough for experiments. The tumor tissues were resected for histology, and cancer Cells were harvested for culture. The lentivirus-transduced MDA-MB-231 Cells could stably express luc2 and eGFP, and the luciferase activity reached 9689 photons/sec/Cell. Meanwhile, no significant difference in biological activities was observed between the lentivirus-transduced MDA-MB-231 Cells and parental Cells. An orthotopically implanted tumor model of human TNBCs was successfully established in BALB/c nude mice. Lentiviruses may be ideal carriers for luciferase genes due to their highly efficient infectivity and stable transgene expression. The modified MDA-MB-231 Cell Line stably expressing luciferase could be detected, allowing for immediate and sensitive detection of metastasis sites in nude mice. As the eGFP and luc2 combination are superior to single reporter genes in their ability to mark Cells in vivo and in vitro, these Cells may provide a visualizable, convenient and sensitive platform for research on the mechanisms of metastasis and the development of new antitumor drugs for human TNBC.
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Establishment of a bioluminescent MDA-MB-231 Cell Line for in vivo imaging of human triple-negative breast cancer xenograft
Journal of Southern Medical University, 2011Co-Authors: Ke Wang, Jianjun He, Xin-wei ZhangAbstract:OBJECTIVE: To establish a bioluminescent MDA-MB-231 Cell Line which can stably express luciferase and green fluorescent protein to allow bioluminescent imaging in nude mouse models bearing human triple-negative breast cancer xenografts. METHODS: The lentivirus carrying luc2, eGFP and neo fusion genes were packaged in 293T Cells via calcium phosphate co-precipitation. Human triple-negative breast cancer Cell Line MDA-MB-231 was infected by the lentivirus, and the positive Cell clones were tested for eGFP and luc2 expressions by fluorescence microscopy and Xenogen IVIS200 bioluminescent imaging system, respectively. MTT assay, transwell invasion assay and wound healing assay were performed to evaluate the changes in the proliferation, invasion and migration abilities of the infected Cells. The Cells were then orthotopically implanted into the right second mammary fat pat of female BALB/c nude mice. The tumor growth was monitored by the in vivo imaging system every week, and the tumor tissues were harvested to evaluate the in vivo stability and tumorigenicity of the modified Cells using cryosection and HE staining. RESULTS: The lentivirus-infected MDA-MB-231Cells could stably express luc2 and eGFP, and the luciferase activity reached 9689 phontons/s/per Cell. No significant changes occurred in the biological activities of the lentivirus-infected MDA-MB-231 Cells. We successfully established the nude mouse model bearing orthotopically implanted human triple-negative breast cancer Cells. CONCLUSION: The modified MDA-MB-231 Cell Line can be detected sensitively at the primary implantation site and distant metastasis site in nude mice, which provides a convenient and sensitive platform for the research of metastatic mechanism and new antitumor drugs of human triple-negative breast cancer. The combination of eGFP and luc2 is superior to single reporter gene.
Jianjun He - One of the best experts on this subject based on the ideXlab platform.
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Establishment of a bioluminescent MDA-MB-231 Cell Line for human triple-negative breast cancer research.
Oncology Reports, 2012Co-Authors: Ke Wang, Xin-wei Zhang, Jianjun HeAbstract:The aim of this study was to establish a bioluminescent MDA-MB-231 Cell Line stably expressing luciferase and green fluorescent protein for the generation of a xenografted model of human triple-negative breast cancer (TNBC) in nude mice. Lentivirus vectors carrying eGFP, firefly luc2 and neo fusion genes were used to transduce the MDA-MB-231 human TNBC Cells in vitro. After 8 weeks of G418 selection, eGFP and luc2 expression was determined using a fluorescence microscope and a Xenogen IVIS200 bioluminescent imaging system, respectively. The MTT, transwell invasion and wound healing assays were performed to confirm whether Cellular proliferation, invasion and migration were altered by lentiviral infection. Cells were orthotopically implanted into female BALB/c nude mice to test the sensitivity and stability of reporter gene expression. Growth of the tumors was monitored with the in vivo imaging system once a week until they were large enough for experiments. The tumor tissues were resected for histology, and cancer Cells were harvested for culture. The lentivirus-transduced MDA-MB-231 Cells could stably express luc2 and eGFP, and the luciferase activity reached 9689 photons/sec/Cell. Meanwhile, no significant difference in biological activities was observed between the lentivirus-transduced MDA-MB-231 Cells and parental Cells. An orthotopically implanted tumor model of human TNBCs was successfully established in BALB/c nude mice. Lentiviruses may be ideal carriers for luciferase genes due to their highly efficient infectivity and stable transgene expression. The modified MDA-MB-231 Cell Line stably expressing luciferase could be detected, allowing for immediate and sensitive detection of metastasis sites in nude mice. As the eGFP and luc2 combination are superior to single reporter genes in their ability to mark Cells in vivo and in vitro, these Cells may provide a visualizable, convenient and sensitive platform for research on the mechanisms of metastasis and the development of new antitumor drugs for human TNBC.
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Establishment of a bioluminescent MDA-MB-231 Cell Line for in vivo imaging of human triple-negative breast cancer xenograft
Journal of Southern Medical University, 2011Co-Authors: Ke Wang, Jianjun He, Xin-wei ZhangAbstract:OBJECTIVE: To establish a bioluminescent MDA-MB-231 Cell Line which can stably express luciferase and green fluorescent protein to allow bioluminescent imaging in nude mouse models bearing human triple-negative breast cancer xenografts. METHODS: The lentivirus carrying luc2, eGFP and neo fusion genes were packaged in 293T Cells via calcium phosphate co-precipitation. Human triple-negative breast cancer Cell Line MDA-MB-231 was infected by the lentivirus, and the positive Cell clones were tested for eGFP and luc2 expressions by fluorescence microscopy and Xenogen IVIS200 bioluminescent imaging system, respectively. MTT assay, transwell invasion assay and wound healing assay were performed to evaluate the changes in the proliferation, invasion and migration abilities of the infected Cells. The Cells were then orthotopically implanted into the right second mammary fat pat of female BALB/c nude mice. The tumor growth was monitored by the in vivo imaging system every week, and the tumor tissues were harvested to evaluate the in vivo stability and tumorigenicity of the modified Cells using cryosection and HE staining. RESULTS: The lentivirus-infected MDA-MB-231Cells could stably express luc2 and eGFP, and the luciferase activity reached 9689 phontons/s/per Cell. No significant changes occurred in the biological activities of the lentivirus-infected MDA-MB-231 Cells. We successfully established the nude mouse model bearing orthotopically implanted human triple-negative breast cancer Cells. CONCLUSION: The modified MDA-MB-231 Cell Line can be detected sensitively at the primary implantation site and distant metastasis site in nude mice, which provides a convenient and sensitive platform for the research of metastatic mechanism and new antitumor drugs of human triple-negative breast cancer. The combination of eGFP and luc2 is superior to single reporter gene.