The Experts below are selected from a list of 21288 Experts worldwide ranked by ideXlab platform
Christoph Westerhausen - One of the best experts on this subject based on the ideXlab platform.
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acoustotaxis in vitro stimulation in a Wound Healing Assay employing surface acoustic waves
Biomaterials Science, 2016Co-Authors: Manuel S. Brugger, Achim Wixforth, Christoph Westerhausen, Melanie StampAbstract:A novel, ultrasound based approach for the dynamic stimulation and promotion of tissue Healing processes employing surface acoustic waves (SAW) on a chip is presented for the example of osteoblast-like SaOs-2 cells. In our investigations, we directly irradiate cells with SAW on a SiO2 covered piezoelectric LiNbO3 substrate. Observing the temporal evolution of cell growth and migration and comparing non-irradiated to irradiated areas on the chip, we find that the SAW–treated cells exhibit a significantly increased migration as compared to the control samples. Apart from quantifying our experimental findings on the cell migration stimulation, we also demonstrate the full bio compatibility and bio functionality of our SAW technique by using LDH Assays. We safely exclude parasitic side effects such as a SAW related increased substrate temperature or nutrient flow by thoroughly monitoring the temperature and the flow field using infrared microscopy and micro particle image velocimetry. Our results show that the SAW induced dynamic mechanical and electrical stimulation obviously directly promotes the cell growth. We conclude that this stimulation method offers a powerful platform for future medical treatment, e.g. being implemented as a implantable biochip with wireless extra-corporal power supply to treat deeper tissue.
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Acoustotaxis – in vitro stimulation in a Wound Healing Assay employing surface acoustic waves
Biomaterials science, 2016Co-Authors: Melanie E. M. Stamp, Manuel S. Brugger, Achim Wixforth, Christoph WesterhausenAbstract:A novel, ultrasound based approach for the dynamic stimulation and promotion of tissue Healing processes employing surface acoustic waves (SAW) on a chip is presented for the example of osteoblast-like SaOs-2 cells. In our investigations, we directly irradiate cells with SAW on a SiO2 covered piezoelectric LiNbO3 substrate. Observing the temporal evolution of cell growth and migration and comparing non-irradiated to irradiated areas on the chip, we find that the SAW–treated cells exhibit a significantly increased migration as compared to the control samples. Apart from quantifying our experimental findings on the cell migration stimulation, we also demonstrate the full bio compatibility and bio functionality of our SAW technique by using LDH Assays. We safely exclude parasitic side effects such as a SAW related increased substrate temperature or nutrient flow by thoroughly monitoring the temperature and the flow field using infrared microscopy and micro particle image velocimetry. Our results show that the SAW induced dynamic mechanical and electrical stimulation obviously directly promotes the cell growth. We conclude that this stimulation method offers a powerful platform for future medical treatment, e.g. being implemented as a implantable biochip with wireless extra-corporal power supply to treat deeper tissue.
Achim Wixforth - One of the best experts on this subject based on the ideXlab platform.
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acoustotaxis in vitro stimulation in a Wound Healing Assay employing surface acoustic waves
Biomaterials Science, 2016Co-Authors: Manuel S. Brugger, Achim Wixforth, Christoph Westerhausen, Melanie StampAbstract:A novel, ultrasound based approach for the dynamic stimulation and promotion of tissue Healing processes employing surface acoustic waves (SAW) on a chip is presented for the example of osteoblast-like SaOs-2 cells. In our investigations, we directly irradiate cells with SAW on a SiO2 covered piezoelectric LiNbO3 substrate. Observing the temporal evolution of cell growth and migration and comparing non-irradiated to irradiated areas on the chip, we find that the SAW–treated cells exhibit a significantly increased migration as compared to the control samples. Apart from quantifying our experimental findings on the cell migration stimulation, we also demonstrate the full bio compatibility and bio functionality of our SAW technique by using LDH Assays. We safely exclude parasitic side effects such as a SAW related increased substrate temperature or nutrient flow by thoroughly monitoring the temperature and the flow field using infrared microscopy and micro particle image velocimetry. Our results show that the SAW induced dynamic mechanical and electrical stimulation obviously directly promotes the cell growth. We conclude that this stimulation method offers a powerful platform for future medical treatment, e.g. being implemented as a implantable biochip with wireless extra-corporal power supply to treat deeper tissue.
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Acoustotaxis – in vitro stimulation in a Wound Healing Assay employing surface acoustic waves
Biomaterials science, 2016Co-Authors: Melanie E. M. Stamp, Manuel S. Brugger, Achim Wixforth, Christoph WesterhausenAbstract:A novel, ultrasound based approach for the dynamic stimulation and promotion of tissue Healing processes employing surface acoustic waves (SAW) on a chip is presented for the example of osteoblast-like SaOs-2 cells. In our investigations, we directly irradiate cells with SAW on a SiO2 covered piezoelectric LiNbO3 substrate. Observing the temporal evolution of cell growth and migration and comparing non-irradiated to irradiated areas on the chip, we find that the SAW–treated cells exhibit a significantly increased migration as compared to the control samples. Apart from quantifying our experimental findings on the cell migration stimulation, we also demonstrate the full bio compatibility and bio functionality of our SAW technique by using LDH Assays. We safely exclude parasitic side effects such as a SAW related increased substrate temperature or nutrient flow by thoroughly monitoring the temperature and the flow field using infrared microscopy and micro particle image velocimetry. Our results show that the SAW induced dynamic mechanical and electrical stimulation obviously directly promotes the cell growth. We conclude that this stimulation method offers a powerful platform for future medical treatment, e.g. being implemented as a implantable biochip with wireless extra-corporal power supply to treat deeper tissue.
Martin Oberringer - One of the best experts on this subject based on the ideXlab platform.
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bromelain down regulates myofibroblast differentiation in an in vitro Wound Healing Assay
Naunyn-schmiedebergs Archives of Pharmacology, 2013Co-Authors: Kathrin Aichele, Monika Bubel, Gunther Deubel, Tim Pohlemann, Martin OberringerAbstract:Bromelain, a pineapple-derived enzyme mixture, is a widely used drug to improve tissue regeneration. Clinical and experimental data indicate a better outcome of soft tissue Healing under the influence of bromelain. Proteolytic, anti-bacterial, anti-inflammatory, and anti-oedematogenic effects account for this improvement on the systemic level. It remains unknown, whether involved tissue cells are directly influenced by bromelain. In order to gain more insight into those mechanisms by which bromelain modulates tissue regeneration at the cellular level, we applied a well-established in vitro Wound Healing Assay. Two main players of soft tissue Healing—fibroblasts and microvascular endothelial cells—were used as mono- and co-cultures. Cell migration, proliferation, apoptosis, and the differentiation of fibroblasts to myofibroblasts as well as interleukin-6 were quantified in response to bromelain (36 × 10−3 IU/ml) under normoxia and hypoxia. Bromelain attenuated endothelial cell and fibroblast proliferation in a moderate way. This proliferation decrease was not caused by apoptosis, rather, by driving cells into the resting state G0 of the cell cycle. Endothelial cell migration was not influenced by bromelain, whereas fibroblast migration was clearly slowed down, especially under hypoxia. Bromelain led to a significant decrease of myofibroblasts under both normoxic (from 19 to 12 %) and hypoxic conditions (from 22 to 15 %), coincident with higher levels of interleukin-6. Myofibroblast differentiation, a clear sign of fibrotic development, can be attenuated by the application of bromelain in vitro. Usage of bromelain as a therapeutic drug for chronic human Wounds thus remains a very promising concept for the future.
Jian Chen - One of the best experts on this subject based on the ideXlab platform.
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A microfabricated 96-well Wound-Healing Assay.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2017Co-Authors: Shaoliang Luan, Rui Hao, Yuanchen Wei, Deyong Chen, Beiyuan Fan, Fengliang Dong, Wei Guo, Junbo Wang, Jian ChenAbstract:This article presents a microfabricated 96-well Wound-Healing Assay enabling high-throughput measurement of cellular migration capabilities. Within each well, the middle area is the Wound region, made of microfabricated gold surface with self-assembled PEG repellent for cell seeding. After the formation of a cellular confluent monolayer around the Wound region, collagen solution was applied to form three-dimensional matrix to cover the PEG surface, initiating the Wound-Healing process. By interpreting the numbers of migrated cells into the Wound regions as a function of specific stimuli with different concentrations, EC50 (half-maximal effective concentration) was obtained. Using H1299 as a model, values of EC50 were quantified as 8% and 160 ng/ml for fetal bovine serum and CXCL12, respectively. In addition, the values of EC50 were demonstrated not to be affected by variations in compositions of extracellular matrix and geometries of Wounds, which can thus be regarded as an intrinsic marker. Furthermore, the migration capabilities of a second cell type (HeLa) were characterized by the developed Wound-Healing Assay, producing EC50 of 2% when fetal bovine serum was used as the stimuli. These results validated the proposed high-throughput Wound-Healing Assay, which may function as an enabling tool in studying cellular capabilities of migration and invasion. © 2017 International Society for Advancement of Cytometry.
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A Tubing-Free Microfluidic Wound Healing Assay Enabling the Quantification of Vascular Smooth Muscle Cell Migration.
Scientific reports, 2015Co-Authors: Yuanchen Wei, Deyong Chen, Wei Guo, Junbo Wang, F. Chen, T. Zhang, Xin Jia, Jian ChenAbstract:This paper presents a tubing-free microfluidic Wound Healing Assay to quantify the migration of vascular smooth muscle cells (VSMCs), where gravity was used to generate a laminar flow within microfluidic channels, enabling cell seeding, culture, and Wound generation. As the first systemic study to quantify the migration of VSMCs within microfluidic environments, the effects of channel geometries, surface modifications and chemokines on cellular migration were investigated, revealing that 1) height of the micro channels had a significant impact on cell migration; 2) the surface coating of collagen induced more migration of VSMCs than fibronectin coated surfaces and 3) platelet derived growth factor resulted in maximal cell migration compared to tumor necrosis factor alpha and fetal bovine serum. Furthermore, migrations of five types of VSMCs (e.g., the human vascular smooth muscle cell line, two types of primary vascular smooth cells, and VSMCs isolated from two human samples) were quantified, finding that VSMCs from the cell line and human samples demonstrated comparable migration distances, which were significantly lower than the migration distances of two primary cell types. As a platform technology, this Wound Healing Assay may function as a new model to study migration of VSMCs within microfluidic environments.
Melanie Stamp - One of the best experts on this subject based on the ideXlab platform.
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acoustotaxis in vitro stimulation in a Wound Healing Assay employing surface acoustic waves
Biomaterials Science, 2016Co-Authors: Manuel S. Brugger, Achim Wixforth, Christoph Westerhausen, Melanie StampAbstract:A novel, ultrasound based approach for the dynamic stimulation and promotion of tissue Healing processes employing surface acoustic waves (SAW) on a chip is presented for the example of osteoblast-like SaOs-2 cells. In our investigations, we directly irradiate cells with SAW on a SiO2 covered piezoelectric LiNbO3 substrate. Observing the temporal evolution of cell growth and migration and comparing non-irradiated to irradiated areas on the chip, we find that the SAW–treated cells exhibit a significantly increased migration as compared to the control samples. Apart from quantifying our experimental findings on the cell migration stimulation, we also demonstrate the full bio compatibility and bio functionality of our SAW technique by using LDH Assays. We safely exclude parasitic side effects such as a SAW related increased substrate temperature or nutrient flow by thoroughly monitoring the temperature and the flow field using infrared microscopy and micro particle image velocimetry. Our results show that the SAW induced dynamic mechanical and electrical stimulation obviously directly promotes the cell growth. We conclude that this stimulation method offers a powerful platform for future medical treatment, e.g. being implemented as a implantable biochip with wireless extra-corporal power supply to treat deeper tissue.
