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Jenny Carlsson - One of the best experts on this subject based on the ideXlab platform.

  • Separation of Meat juice from various animals using a lectin panel and ellipsometry
    2013
    Co-Authors: Jenny Carlsson, Fredrik Winquist, Bengt Danielsson
    Abstract:

    In this thesis lectin panels on gold wafers were utilized to investigate sera from different mammals and Meat Juices from various species of animals. Lectins, which are a group of diverse, natural proteins recognize and bind to different carbohydrate structures present on the sera proteins and on the proteins in the different Meat Juices. The binding patterns from the relatively unspecific interactions between lectins and carbohydrate structures were evaluated with techniques based on ellipsometry. The resulting data was treated with multivariate data analysis (MVDA) techniques, especially principal component analysis (PCA), to identify separation or grouping of data.It was shown that lectin panels combined with ellipsometric techniques and MVDA could be used to separate sera from different mammals and also a possible relation between species could be seen. Meat Juices from the different species evaluated were also possible to separate and using a simple model based on an artificial neuronal net, it was also possible to classify Meat Juices from the mammals investigated. In spite of its great analytical potential, not much work has yet been done utilizing MVDA together with bioanalytical methods. The results in this thesis however, show great potential for combining lectin panels with MVDA.This work, regarding lectin panels, was incorporated as a part of a EU-project, Nanocell. The aim of the Nanocell-project was to develop a biosensor consisting of a single biomolecule electrically interfaced to nanoelectrodes, which is sensed electronically/optically.

  • Biosensor discrimination of Meat juice from various animals using a lectin panel and ellipsometry
    Analytica Chimica Acta, 2005
    Co-Authors: Jenny Carlsson, Fredrik Winquist, Bengt Danielsson, Ingemar Lundström
    Abstract:

    In this work, simple microcontact printed gold-wafers were used to make a lectin panel for investigation and discrimination of different Meat Juices from fresh Meat of cattle, chicken, pig, cod, turkey and lamb. Seven different lectins were thus attached to gold surfaces using the streptavidin–biotin method. Lectins recognize and bind specifically to carbohydrate structures present on different proteins. The biorecognition was evaluated with null ellipsometry and the data obtained was related to an internal standard of lactoferrin. The data was evaluated with multivariate data analysis techniques to identify possible discrimination or grouping of data. Scanning ellipsometry was used for visualization of the binding pattern of the lectins and the Meat juice proteins. The two-dimensional images obtained could be used to visualize the protein distribution, furthermore, to exclude anomalies. The results showed that the different Meat Juices from the six different species: cattle, chicken, pig, cod, turkey and lamb could be discriminated from each other. The results showed to be more repetitive for the mammalian Meat Juices. Using a simple model based on an artificial neuronal net, it was also possible to classify Meat Juices from the mammals investigated.

  • Surface analytical methods and multivariate data analysis applied to lectin panels
    2004
    Co-Authors: Jenny Carlsson
    Abstract:

    In this thesis lectin panels on gold wafers were utilized to investigate sera from different mammals and Meat Juices from various species of animals. Lectins, which are a group of diverse, natural ...

Stéphane Bretagne - One of the best experts on this subject based on the ideXlab platform.

  • Seroprevalence of Toxoplasma gondii and direct genotyping using minisequencing in free-range pigs in Burkina Faso
    International Journal of Food Microbiology, 2016
    Co-Authors: Sanata Bamba, Lénaïg Halos, Zékiba Tarnagda, Alexandre Alanio, Pauline Macé, Sandrine Moukoury, Ibrahim Sangaré, Robert Guiguemdé, Jean-marc Costa, Stéphane Bretagne
    Abstract:

    BACKGROUND: Swine are a major source of Meat for humans. As such, they can play an important role in the epidemiology of human toxoplasmosis. Therefore, we performed an epidemiological study to determine the prevalence and genotypes of Toxoplasma gondii in Burkina Fasan swine. METHODS: The prevalence of T. gondii infection was evaluated in a 3-month prospective study at the slaughterhouse of Bobo-Dioulasso, Burkina Faso. Anti-Toxoplasma IgG titers were determined on Meat Juices from pig diaphragms using a commercially available ELISA assay. The DNA was extracted from 25mg of heart biopsies of seropositive animals (IgG ≥50% of the control) and the presence of T. gondii DNA was detected using a quantitative PCR assay. Genotyping was performed directly on DNA from PCR-positive biopsies using high-resolution melting and minisequencing analyses of the repeated B1 gene. RESULTS: The prevalence of carcasses positive for anti-Toxoplasma IgG was 29% (87/300) with no difference according to sex and age in contrast to the village of origin (p=0.018). Of the 87 seropositive animals, two were PCR positive (parasitic load at 64 and 128 parasites/mg of heart biopsy). Two new genotypes belonging to Type II and Type III and different from the genotypes previously described using minisequencing were identified. CONCLUSION: Our study provides the first T. gondii seroprevalence data in Burkina Fasan swine. In addition, this direct typing method suggests diversity of the T. gondii genotypes circulating in domestic animals in Burkina Faso. This needs to be confirmed on a wider sampling of subjects.

Bengt Danielsson - One of the best experts on this subject based on the ideXlab platform.

  • Separation of Meat juice from various animals using a lectin panel and ellipsometry
    2013
    Co-Authors: Jenny Carlsson, Fredrik Winquist, Bengt Danielsson
    Abstract:

    In this thesis lectin panels on gold wafers were utilized to investigate sera from different mammals and Meat Juices from various species of animals. Lectins, which are a group of diverse, natural proteins recognize and bind to different carbohydrate structures present on the sera proteins and on the proteins in the different Meat Juices. The binding patterns from the relatively unspecific interactions between lectins and carbohydrate structures were evaluated with techniques based on ellipsometry. The resulting data was treated with multivariate data analysis (MVDA) techniques, especially principal component analysis (PCA), to identify separation or grouping of data.It was shown that lectin panels combined with ellipsometric techniques and MVDA could be used to separate sera from different mammals and also a possible relation between species could be seen. Meat Juices from the different species evaluated were also possible to separate and using a simple model based on an artificial neuronal net, it was also possible to classify Meat Juices from the mammals investigated. In spite of its great analytical potential, not much work has yet been done utilizing MVDA together with bioanalytical methods. The results in this thesis however, show great potential for combining lectin panels with MVDA.This work, regarding lectin panels, was incorporated as a part of a EU-project, Nanocell. The aim of the Nanocell-project was to develop a biosensor consisting of a single biomolecule electrically interfaced to nanoelectrodes, which is sensed electronically/optically.

  • Biosensor discrimination of Meat juice from various animals using a lectin panel and ellipsometry
    Analytica Chimica Acta, 2005
    Co-Authors: Jenny Carlsson, Fredrik Winquist, Bengt Danielsson, Ingemar Lundström
    Abstract:

    In this work, simple microcontact printed gold-wafers were used to make a lectin panel for investigation and discrimination of different Meat Juices from fresh Meat of cattle, chicken, pig, cod, turkey and lamb. Seven different lectins were thus attached to gold surfaces using the streptavidin–biotin method. Lectins recognize and bind specifically to carbohydrate structures present on different proteins. The biorecognition was evaluated with null ellipsometry and the data obtained was related to an internal standard of lactoferrin. The data was evaluated with multivariate data analysis techniques to identify possible discrimination or grouping of data. Scanning ellipsometry was used for visualization of the binding pattern of the lectins and the Meat juice proteins. The two-dimensional images obtained could be used to visualize the protein distribution, furthermore, to exclude anomalies. The results showed that the different Meat Juices from the six different species: cattle, chicken, pig, cod, turkey and lamb could be discriminated from each other. The results showed to be more repetitive for the mammalian Meat Juices. Using a simple model based on an artificial neuronal net, it was also possible to classify Meat Juices from the mammals investigated.

Sanata Bamba - One of the best experts on this subject based on the ideXlab platform.

  • Seroprevalence of Toxoplasma gondii and direct genotyping using minisequencing in free-range pigs in Burkina Faso
    International Journal of Food Microbiology, 2016
    Co-Authors: Sanata Bamba, Lénaïg Halos, Zékiba Tarnagda, Alexandre Alanio, Pauline Macé, Sandrine Moukoury, Ibrahim Sangaré, Robert Guiguemdé, Jean-marc Costa, Stéphane Bretagne
    Abstract:

    BACKGROUND: Swine are a major source of Meat for humans. As such, they can play an important role in the epidemiology of human toxoplasmosis. Therefore, we performed an epidemiological study to determine the prevalence and genotypes of Toxoplasma gondii in Burkina Fasan swine. METHODS: The prevalence of T. gondii infection was evaluated in a 3-month prospective study at the slaughterhouse of Bobo-Dioulasso, Burkina Faso. Anti-Toxoplasma IgG titers were determined on Meat Juices from pig diaphragms using a commercially available ELISA assay. The DNA was extracted from 25mg of heart biopsies of seropositive animals (IgG ≥50% of the control) and the presence of T. gondii DNA was detected using a quantitative PCR assay. Genotyping was performed directly on DNA from PCR-positive biopsies using high-resolution melting and minisequencing analyses of the repeated B1 gene. RESULTS: The prevalence of carcasses positive for anti-Toxoplasma IgG was 29% (87/300) with no difference according to sex and age in contrast to the village of origin (p=0.018). Of the 87 seropositive animals, two were PCR positive (parasitic load at 64 and 128 parasites/mg of heart biopsy). Two new genotypes belonging to Type II and Type III and different from the genotypes previously described using minisequencing were identified. CONCLUSION: Our study provides the first T. gondii seroprevalence data in Burkina Fasan swine. In addition, this direct typing method suggests diversity of the T. gondii genotypes circulating in domestic animals in Burkina Faso. This needs to be confirmed on a wider sampling of subjects.

Laurent Debrauwer - One of the best experts on this subject based on the ideXlab platform.

  • Formation of heterocyclic amines in slices of longissimus thoracis beef muscle subjected to jets of superheated steam
    Food Chemistry, 2010
    Co-Authors: Alain Kondjoyan, Sylvie Chevolleau-mege, Eléonore Greve, Philippe Gatellier, Veronique Sante-lhoutellier, Sandrine Bruel, Catherine Touzet, Stéphane Portanguen, Laurent Debrauwer
    Abstract:

    The kinetics of the formation of heterocyclic amines (HA) were measured on slices of Longissimus thoracis (LT) muscle subjected to impinging jets of superheated steam. Product temperature was either 170 or 200 degrees C and treatment duration ranged from 1 to 20 min. The concentrations of lQx, MelQx, 4,8-DiMelQx and PhIP followed regular kinetic patterns. HA formation increased significantly between 170 and 200 degrees C. The quantities of lQx and 4,8-DiMelQx formed in LT slices were 3 to 4-fold smaller than those formed in Meat Juices, while quantities of MelQx and PhIP remained comparable. A first-order kinetic model taken from the literature was adapted to describe the results taking into account product temperature variations over the course of the experiment.