The Experts below are selected from a list of 18 Experts worldwide ranked by ideXlab platform
Hongfeng Yin - One of the best experts on this subject based on the ideXlab platform.
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improved lc ms methods for the analysis of metal sensitive analytes using Medronic Acid as a mobile phase additive
Analytical Chemistry, 2018Co-Authors: Jordy J Hsiao, Oscar G Potter, Te Wei Chu, Hongfeng YinAbstract:Phosphorylated compounds and organic Acids with multiple carboxylate groups are commonly observed to have poor peak shapes and signal in LC/MS experiments. The poor peak shape is caused by the presence of trace metals, particularly iron, contributed from a variety of sources within the chromatographic system. To ameliorate this problem, different solvent additives were investigated to reduce the amount of metal in the flow path to achieve better analytical performance for these metal-sensitive compounds. Here, we introduce the use of a solvent additive that can significantly improve the peak shapes and signal of metal-sensitive metabolites for LC/MS analysis. Moreover, the additive is shown to be amenable for other metal-sensitive applications, such as the analysis of phosphopeptides and polar phosphorylated pesticides, where the instruments could be used in either positive or negative analysis mode.
Jordy J Hsiao - One of the best experts on this subject based on the ideXlab platform.
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improved lc ms methods for the analysis of metal sensitive analytes using Medronic Acid as a mobile phase additive
Analytical Chemistry, 2018Co-Authors: Jordy J Hsiao, Oscar G Potter, Te Wei Chu, Hongfeng YinAbstract:Phosphorylated compounds and organic Acids with multiple carboxylate groups are commonly observed to have poor peak shapes and signal in LC/MS experiments. The poor peak shape is caused by the presence of trace metals, particularly iron, contributed from a variety of sources within the chromatographic system. To ameliorate this problem, different solvent additives were investigated to reduce the amount of metal in the flow path to achieve better analytical performance for these metal-sensitive compounds. Here, we introduce the use of a solvent additive that can significantly improve the peak shapes and signal of metal-sensitive metabolites for LC/MS analysis. Moreover, the additive is shown to be amenable for other metal-sensitive applications, such as the analysis of phosphopeptides and polar phosphorylated pesticides, where the instruments could be used in either positive or negative analysis mode.
Petri A Turhanen - One of the best experts on this subject based on the ideXlab platform.
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synthesis of Medronic Acid monoesters and their purification by high performance countercurrent chromatography or by hydroxyapatite
Beilstein Journal of Organic Chemistry, 2016Co-Authors: Elina Puljula, Jouko Vepsalainen, Petri A TurhanenAbstract:We achieved the synthesis of important Medronic Acid monoalkyl esters via the dealkylation of mixed trimethyl monoalkyl esters of Medronic Acid. Two methods were developed for the purification of Medronic Acid monoesters: 1) small scale (10–20 mg) purification by using hydroxyapatite and 2) large scale (tested up to 140 mg) purification by high-performance countercurrent chromatography (HPCCC).
Te Wei Chu - One of the best experts on this subject based on the ideXlab platform.
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improved lc ms methods for the analysis of metal sensitive analytes using Medronic Acid as a mobile phase additive
Analytical Chemistry, 2018Co-Authors: Jordy J Hsiao, Oscar G Potter, Te Wei Chu, Hongfeng YinAbstract:Phosphorylated compounds and organic Acids with multiple carboxylate groups are commonly observed to have poor peak shapes and signal in LC/MS experiments. The poor peak shape is caused by the presence of trace metals, particularly iron, contributed from a variety of sources within the chromatographic system. To ameliorate this problem, different solvent additives were investigated to reduce the amount of metal in the flow path to achieve better analytical performance for these metal-sensitive compounds. Here, we introduce the use of a solvent additive that can significantly improve the peak shapes and signal of metal-sensitive metabolites for LC/MS analysis. Moreover, the additive is shown to be amenable for other metal-sensitive applications, such as the analysis of phosphopeptides and polar phosphorylated pesticides, where the instruments could be used in either positive or negative analysis mode.
Oscar G Potter - One of the best experts on this subject based on the ideXlab platform.
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improved lc ms methods for the analysis of metal sensitive analytes using Medronic Acid as a mobile phase additive
Analytical Chemistry, 2018Co-Authors: Jordy J Hsiao, Oscar G Potter, Te Wei Chu, Hongfeng YinAbstract:Phosphorylated compounds and organic Acids with multiple carboxylate groups are commonly observed to have poor peak shapes and signal in LC/MS experiments. The poor peak shape is caused by the presence of trace metals, particularly iron, contributed from a variety of sources within the chromatographic system. To ameliorate this problem, different solvent additives were investigated to reduce the amount of metal in the flow path to achieve better analytical performance for these metal-sensitive compounds. Here, we introduce the use of a solvent additive that can significantly improve the peak shapes and signal of metal-sensitive metabolites for LC/MS analysis. Moreover, the additive is shown to be amenable for other metal-sensitive applications, such as the analysis of phosphopeptides and polar phosphorylated pesticides, where the instruments could be used in either positive or negative analysis mode.