The Experts below are selected from a list of 2409 Experts worldwide ranked by ideXlab platform
Simona Collina - One of the best experts on this subject based on the ideXlab platform.
-
microwave assisted extraction of coumarin and related compounds from Melilotus officinalis l pallas as an alternative to soxhlet and ultrasound assisted extraction
Journal of Chromatography A, 2006Co-Authors: Emanuela Martino, Ilaria Ramaiola, Mariangela Urbano, Francesco Bracco, Simona CollinaAbstract:Soxhlet extraction, ultrasound-assisted extraction (USAE) and microwaves-assisted extraction (MAE) in closed system have been investigated to determine the content of coumarin, o-coumaric and melilotic acids in flowering tops of Melilotus officinalis. The extracts were analyzed with an appropriate HPLC procedure. The reproducibility of extraction and of chromatographic analysis was proved. Taking into account the extraction yield, the cost and the time, we studied the effects of extraction variables on the yield of the above-mentioned compounds. Better results were obtained with MAE (50% v/v aqueous ethanol, two heating cycles of 5 min, 50 °C). On the basis of the ratio extraction yield/extraction time, we therefore propose MAE as the most efficient method.
-
microwave assisted extraction of coumarin and related compounds from Melilotus officinalis l pallas as an alternative to soxhlet and ultrasound assisted extraction
Journal of Chromatography A, 2006Co-Authors: Emanuela Martino, Ilaria Ramaiola, Mariangela Urbano, Francesco Bracco, Simona CollinaAbstract:Soxhlet extraction, ultrasound-assisted extraction (USAE) and microwaves-assisted extraction (MAE) in closed system have been investigated to determine the content of coumarin, o-coumaric and melilotic acids in flowering tops of Melilotus officinalis. The extracts were analyzed with an appropriate HPLC procedure. The reproducibility of extraction and of chromatographic analysis was proved. Taking into account the extraction yield, the cost and the time, we studied the effects of extraction variables on the yield of the above-mentioned compounds. Better results were obtained with MAE (50% v/v aqueous ethanol, two heating cycles of 5 min, 50 degrees C). On the basis of the ratio extraction yield/extraction time, we therefore propose MAE as the most efficient method.
Jiyu Zhang - One of the best experts on this subject based on the ideXlab platform.
-
genetic diversity phylogenetic structure and development of core collections in Melilotus accessions from a chinese gene bank
Scientific Reports, 2019Co-Authors: Hongxiang Zhang, Fan Wu, Yufei Zhang, Jiyu ZhangAbstract:Melilotus is an important forage legume, with high values as feed and medicine, and widely used as green manure, honey plant, and wildlife habitat enhancer. The genetic diversity, structure and subdivision of this forage crop remain unclear, and plant genetic resources are the basis of biodiversity and ecosystem diversity and have attracted increasing attention. In this study, the whole collection of 573 accessions from the National Gene Bank of Forage Germplasm (NGBFG, China) and 48 accessions from the National Plant Germplasm System (NPGS, USA) in genus Melilotus were measured with respect to five seed characters: seed length, width, width-to-length ratio, circumference and 100-seed weight. Shannon’ genetic diversity index (H’) and phenotypic differentiation (Pst) were calculated to better describe the genetic diversity. The ITS and matK sequences were used to construct phylogenetic trees and study the genetic relationships within genus Melilotu. Based on seed morphology and molecular marker data, we preliminarily developed core collections and the sampling rates of M. albus and M. officinalis were determined to be 15% and 25%, respectively. The results obtained here provide preliminary sorting and supplemental information for the Melilotus collections in NGBFG, China, and establish a reference for further genetic breeding and other related projects.
-
coumarin content morphological variation and molecular phylogenetics of Melilotus
Molecules, 2018Co-Authors: Jiyu Zhang, Zhuanzhuan Yan, Kai Luo, Zhen Duan, Zulfi Jahufer, Alan Stewart, Yanrong WangAbstract:Melilotus albus and Melilotus officinalis are widely used in forage production and herbal medicine due to the biological activity of their coumarins, which have many biological and pharmacological activities, including anti-HIV and anti-tumor effects. To comprehensively evaluate M. albus and M. officinalis coumarin content (Cou), morphological variation, and molecular phylogeny, we examined the Cou, five morphological traits and the molecular characterization based on the trnL-F spacer and internal transcribed spacer (ITS) regions of 93 accessions. Significant (p < 0.05) variation was observed in the Cou and all five morphological traits in both species. Analysis of population differentiation (Pst) of the phenotypic traits showed that powdery mildew resistance (PMR) had the greatest Pst, meaning that this trait demonstrated the largest genetic differentiation among the accessions. The Pst values of dry matter yield (DMY) and Cou were relatively high. Biplot analysis identified accessions with higher DMY and higher and lower Cou. Analysis of molecular sequence variation identified seven haplotypes of the trnL-F spacer and 13 haplotypes of the ITS region. Based on haplotype and sequence analyses, the genetic variation of M. officinalis was higher than that of M. albus. Additionally, ITS sequence analysis showed that the variation among accessions was larger than that among species across three geographical areas: Asia, Europe, and North America. Similarly, variation among accessions for both the trnL-F and ITS sequences were larger than the differences between the geographical areas. Our results indicate that there has been considerable gene flow between the two Melilotus species. Our characterization of Cou and the morphological and genetic variations of these two Melilotus species may provide useful insights into germplasm improvement to enhance DMY and Cou.
-
genetic variation and diversity in 199 Melilotus accessions based on a combination of 5 dna sequences
PLOS ONE, 2018Co-Authors: Hongxiang Zhang, Yufei Zhang, Zhuanzhuan Yan, Qi Yan, Blaise Pascal Muvunyi, Wenli Guo, Rong Bai, Jiyu ZhangAbstract:Melilotus is an important genus of legume plants and an herbage with excellent nitrogen fixation; it can tolerate extreme environmental conditions and possesses important medicinal value. However, there is limited genetic information about the genus; thus, we analysed four chloroplast loci (rbcL, matK, psbA-trnH and trnL-F) and one nuclear region (ITS) to determine the genetic diversity of 199 accessions from 18 Melilotus species. The rbcL and matK sequences were highly conserved, whereas the trnL-F and ITS sequences contained variable and parsimony-informative sites. In our analyses of the single and combined regions, we calculated the pairwise distance, haplotype and nucleotide diversity and gaps and then constructed phylogenetic trees to assess the genetic diversity, and our results revealed significant variations among the different accessions. The genetic distance values were between zero and nine, and based on the combined regions, the highest frequency value was approximately four. Melilotus showed high haplotype and nucleotide diversity, particularly in the ITS sequences, with values of 0.86 and 0.0087, respectively. The single ITS sequence, psbA-trnH, and the combined matK+rbcL+trnL-F (MRT) and matK+rbcL+psbA-trnH+trnL-F+ITS (MRPTI) regions showed interspecific variation in the gap analysis. Phylogenetic trees calculated using ITS, psbA-trnH and MRPTI sequences indicated distinct genetic relationship in 18 species, and these species could be divided into two groups. By determining the genetic diversity of plants, we can evaluate the genetic relationships among species and accessions, providing a basis for preserving and utilizing the genetic resources of Melilotus.
-
cross species transferability of est ssr markers developed from the transcriptome of Melilotus and their application to population genetics research
Scientific Reports, 2017Co-Authors: Zhuanzhuan Yan, Yufei Zhang, Kai Luo, Yufeng Zhao, Qi Yan, Yanrong Wang, Jiyu ZhangAbstract:Melilotus is one of the most important legume forages, but the lack of molecular markers has limited the development and utilization of Melilotus germplasm resources. In the present study, 151 M clean reads were generated from various genotypes of Melilotus albus using Illumina sequencing. A total of 19,263 potential EST-SSRs were identified from 104,358 unigene sequences. Moreover, 18,182 primer pairs were successfully designed, and 550 primer pairs were selected using criteria of base repeat type, fragment length and annealing temperature. In addition, 550 primer pairs were screened by using PCR amplification products and used to assess polymorphisms in 15 M. albus accessions. A total of 114 primer pairs were detected as being highly polymorphic, and the average polymorphism information content (PIC) value was 0.79. Furthermore, those 114 polymorphic primer pairs were used to evaluate the transferability to 18 species of the genus Melilotus, and 70 EST-SSR markers were found to be transferable among the 18 Melilotus species. According to the UPGMA dendrogram and STRUCTURE analysis, the 18 Melilotus species were classified into three clusters. This study offers a valuable resource for the genetic diversity and molecular assisted breeding of germplasm resources in the genus Melilotus.
-
potential dna barcodes for Melilotus species based on five single loci and their combinations
PLOS ONE, 2017Co-Authors: Yuqin Meng, Kai Luo, Yanrong Wang, Daiyu Zhang, Blaise Pascal Muvunyi, Wenli Guo, Baochang Feng, Jiyu ZhangAbstract:Melilotus, an annual or biennial herb, belongs to the tribe Trifolieae (Leguminosae) and consists of 19 species. As an important green manure crop, diverse Melilotus species have different values as feed and medicine. To identify different Melilotus species, we examined the efficiency of five candidate regions as barcodes, including the internal transcribed spacer (ITS) and two chloroplast loci, rbcL and matK, and two non-coding loci, trnH-psbA and trnL-F. In total, 198 individuals from 98 accessions representing 18 Melilotus species were sequenced for these five potential barcodes. Based on inter-specific divergence, we analysed sequences and confirmed that each candidate barcode was able to identify some of the 18 species. The resolution of a single barcode and its combinations ranged from 33.33% to 88.89%. Analysis of pairwise distances showed that matK+rbcL+trnL-F+trnH-psbA+ITS (MRTPI) had the greatest value and rbcL the least. Barcode gap values and similarity value analyses confirmed these trends. The results indicated that an ITS region, successfully identifying 13 of 18 species, was the most appropriate single barcode and that the combination of all five potential barcodes identified 16 of the 18 species. We conclude that MRTPI is the most effective tool for Melilotus species identification. Taking full advantage of the barcode system, a clear taxonomic relationship can be applied to identify Melilotus species and enhance their practical production.
Zi-qiang Yuan - One of the best experts on this subject based on the ideXlab platform.
-
medicago sativa improves soil carbon sequestration following revegetation of degraded arable land in a semi arid environment on the loess plateau china
Agriculture Ecosystems & Environment, 2016Co-Authors: Zi-qiang Yuan, Chao Fang, Kailiang Yu, Xiaokang Guan, Ming Li, Fengmin LiAbstract:Abstract Revegetation of degraded arable land with legume pasture has been proposed as an effective approach to improve vegetation conditions and control soil erosion on the Loess Plateau, China. This paper investigated the effects of revegetation on soil carbon pool and its fractions. Three treatments (natural revegetation, Medicago sativa L. introduction and Melilotus suaveolens L. introduction) were applied over 11 years from 2003 to 2013 on the semi-arid Loess Plateau; both aboveground biomass and soil organic carbon (SOC) stocks as well as its fractions (i.e., the light-fraction organic carbon (LFOC) and heavy-fraction organic carbon (HFOC)) were measured. We found that plant aboveground biomass was greater under Melilotus treatment than under Medicago treatment and natural revegetation in the first two years. Afterward, the aboveground biomass was greatest under Medicago treatment. Medicago treatment yielded higher SOC and HFOC stocks than the other two treatments during the experimental period. After revegetation for 10 years, the SOC stocks at the 0–1.5 m soil layer under Medicago treatment were 52.8 and 44.8 Mg ha−1 higher than those under natural revegetation and Melilotus treatments, respectively. The mean rates of SOC and HFOC sequestration in the top 20 cm of the soil layer under Medicago treatment were 0.27 and 0.23 Mg ha−1 year−1 in year 11, which were greater than the other two treatments (0.22, 0.15 and −0.07, −0.03 under the two treatments, respectively). Revegetation through introduction of Medicago to degraded arable land can potentially promote SOC accumulation by enhancing the transformation from LFOC to HFOC on the semi-arid Loess Plateau.
-
Effects of legume species introduction on vegetation and soil nutrient development on abandoned croplands in a semi-arid environment on the Loess Plateau, China.
Science of The Total Environment, 2016Co-Authors: Zi-qiang Yuan, Howard E. Epstein, Chao Fang, Qian-qian Liu, Xue-wei Liu, Wen-juan GaoAbstract:Revegetation facilitated by legume species introduction has been used for soil erosion control on the Loess Plateau, China. However, it is still unclear how vegetation and soil resources develop during this restoration process, especially over the longer term. In this study, we investigated the changes of plant aboveground biomass, vegetation cover, species richness and density of all individuals, and soil total nitrogen, mineral nitrogen, total phosphorus and available phosphorus over 11 years from 2003 to 2013 in three treatments (natural revegetation, Medicago sativa L. introduction and Melilotus suaveolens L. introduction) on the semi-arid Loess Plateau. Medicago significantly increased aboveground biomass and vegetation cover, and soil total nitrogen and mineral nitrogen contents. The Medicago treatment had lower species richness and density of all individuals, lower soil moisture in the deep soil (i.e., 1.4-5m), and lower soil available phosphorus. Melilotus introduction significantly increased aboveground biomass in only the first two years, and it was not an effective approach to improve vegetation biomass and cover, and soil nutrients, especially in later stages of revegetation. Overall, our study suggests that M. sativa can be the preferred plant species for revegetation of degraded ecosystems on the Loess Plateau, although phosphorus fertilizer should be applied for the sustainability of the revegetation.
Jihua Dong - One of the best experts on this subject based on the ideXlab platform.
-
inner anti inflammatory mechanisms of petroleum ether extract from Melilotus suaveolens ledeb
Inflammation, 2007Co-Authors: Lei Zhao, Ran Pang, Shuling Zhang, Jihua DongAbstract:Summary Melilotus suaveolens Ledeb is a species of traditional medical plant for treating inflammation-related disease. This study is to explore the inner anti-inflammatory mechanism on petroleum ether extract from Melilotus suaveolens Ledeb.
-
inner anti inflammatory mechanisms of petroleum ether extract from Melilotus suaveolens ledeb
Inflammation, 2007Co-Authors: Lei Zhao, Jihua Dong, Shuling Zhang, Ran Pang, Junyan Tao, Feng Jin, Yuanjin GuoAbstract:Melilotus suaveolens Ledeb is a species of traditional medical plant for treating inflammation-related disease. This study is to explore the inner anti-inflammatory mechanism on petroleum ether extract from Melilotus suaveolens Ledeb. Inflammatory cellular model was founded by intervention of lipopolysaccharide (LPS) on RAW264.7 cell line. Secretion of TNF-α, IL-1β, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-α, COX-2, iNOS and HO-1, protein expression of COX-2 and HO-1, activation of NF-κB and ingredients in the extract were assayed. The extract could not only reduce production of pro-inflammatory mediators by blocking NF-κB activation but promote release of anti-inflammatory mediator HO-1 significantly. The only active ingredient in the extract was coumarin and the concentration of coumarin in each 1 g extract was 0.27822 mg. Compared to Dexamethasone, the extract not only has similar effects on antagonizing pro-inflammatory mediators and cytokines but has effects on promoting production of anti-inflammatory mediators.
Emanuela Martino - One of the best experts on this subject based on the ideXlab platform.
-
microwave assisted extraction of coumarin and related compounds from Melilotus officinalis l pallas as an alternative to soxhlet and ultrasound assisted extraction
Journal of Chromatography A, 2006Co-Authors: Emanuela Martino, Ilaria Ramaiola, Mariangela Urbano, Francesco Bracco, Simona CollinaAbstract:Soxhlet extraction, ultrasound-assisted extraction (USAE) and microwaves-assisted extraction (MAE) in closed system have been investigated to determine the content of coumarin, o-coumaric and melilotic acids in flowering tops of Melilotus officinalis. The extracts were analyzed with an appropriate HPLC procedure. The reproducibility of extraction and of chromatographic analysis was proved. Taking into account the extraction yield, the cost and the time, we studied the effects of extraction variables on the yield of the above-mentioned compounds. Better results were obtained with MAE (50% v/v aqueous ethanol, two heating cycles of 5 min, 50 °C). On the basis of the ratio extraction yield/extraction time, we therefore propose MAE as the most efficient method.
-
microwave assisted extraction of coumarin and related compounds from Melilotus officinalis l pallas as an alternative to soxhlet and ultrasound assisted extraction
Journal of Chromatography A, 2006Co-Authors: Emanuela Martino, Ilaria Ramaiola, Mariangela Urbano, Francesco Bracco, Simona CollinaAbstract:Soxhlet extraction, ultrasound-assisted extraction (USAE) and microwaves-assisted extraction (MAE) in closed system have been investigated to determine the content of coumarin, o-coumaric and melilotic acids in flowering tops of Melilotus officinalis. The extracts were analyzed with an appropriate HPLC procedure. The reproducibility of extraction and of chromatographic analysis was proved. Taking into account the extraction yield, the cost and the time, we studied the effects of extraction variables on the yield of the above-mentioned compounds. Better results were obtained with MAE (50% v/v aqueous ethanol, two heating cycles of 5 min, 50 degrees C). On the basis of the ratio extraction yield/extraction time, we therefore propose MAE as the most efficient method.