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C. R. Maity - One of the best experts on this subject based on the ideXlab platform.

A. K. Mukherjee - One of the best experts on this subject based on the ideXlab platform.

Jun Miyake - One of the best experts on this subject based on the ideXlab platform.

  • Stabilization of liposomal Membranes by carotenoids: Zeaxanthin, zeaxanthin glucoside and thermozeaxanthin
    Materials Science and Engineering: C, 2008
    Co-Authors: Masayuki Hara, Yumiko Yamano, Yoshitsugu Sakai, Eri Kodama, Takayuki Hoshino, Masayoshi Ito, Jun Miyake
    Abstract:

    We investigated the Membrane-Stabilizing Effect of three synthesized polar carotenoids, thermozeaxanthin (TZ), zeaxanthin glucoside (ZG) and zeaxanthin (Z), using the fluorescent calcein-leakage measurement from the calcein-entrapped liposomes composed of dipalmitoylphosphatidylcholine (DPPC). The addition of TZ stabilized the liposomal Membranes composed of DPPC at pH values ranging from 4.0 to 10.0. The addition of three carotenoids, TZ, ZG and Z, stabilized the Membrane at acidic and neutral pH values ranging from 4.0 to 7.5. The values of leakage were lower at 30 °C and highest at 40 °C, and subsequently, gradually decreased at the higher temperature. The addition of TZ and Z stabilized the Membranes, whereas, ZG destabilized the Membranes at a temperature higher than 50 °C. In addition, the Membrane-Stabilizing Effect of the carotenoids with the calcein-entrapped liposomes composed of lipids extracted from Thermus thermophilus were investigated. The addition of TZ resulted in stabilization of the Membrane at all ranges of pH values. However, the addition of Z and ZG destabilized the Membrane.

S. K. Ghosal - One of the best experts on this subject based on the ideXlab platform.

G. V. Rajamanickam - One of the best experts on this subject based on the ideXlab platform.

  • Antilipoperoxidative and Membrane Stabilizing Effect of diosgenin, in experimentally induced myocardial infarction
    Molecular and Cellular Biochemistry, 2009
    Co-Authors: K. S. Jayachandran, Hannah R. Vasanthi, G. V. Rajamanickam
    Abstract:

    Altered Membrane integrity has been suggested as a major factor in the development of cellular injury during myocardial necrosis. The present study was designed to investigate the Effect of diosgenin on lysosomal hydrolases, Membrane-bound enzymes, and electrolytes during isoproterenol (ISO)-induced myocardial necrosis in rats. Animals were pretreated with DIOS (80 mg/kg) for a period of 35 days. Myocardial infarction was experimentally induced with ISO (85 mg/kg) twice at 24 h interval. Experimental myocardial infarction was evidenced with marked elevation of creatine kinase-MB (CK-MB) in serum with concomitant increase in lipid peroxidation (plasma thiobarbituric acid reactive substances (TBARS) and hydroperoxides (HP)). Activity of lysosomal hydrolases (β-glucuronidase, β- N -acetyl glucosaminidase, β- d -galactosidase, cathepsin D, and acid phosphatase) was found to be increased in serum and heart tissue of ISO-alone treated animals. DIOS (80 mg/kg) pretreated groups showed significant decrease in CK-MB, lipid peroxidation, and lysosomal hydrolases activity. The Membrane-bound enzymes such as Ca^2+-ATPase and Mg^2+-ATPase activity was increased and Na^+/K^+-ATPase activity was decreased in the heart tissues of ISO-alone treated animals. These enzyme alterations lead to the change in the electrolytes content such as sodium, potassium, and calcium in the heart tissue. However, DIOS (80 mg/kg) pretreatment reversed the Membrane-bound enzymes activity and thereby maintained the normal electrolyte concentration. These results suggest the protective action of diosgenin in ISO-induced myocardial infarction. The salubrious Effect observed in this study might be due to the antioxidant and Membrane Stabilizing potential of diosgenin.

  • Antilipoperoxidative and Membrane Stabilizing Effect of diosgenin, in experimentally induced myocardial infarction.
    Molecular and cellular biochemistry, 2009
    Co-Authors: K. S. Jayachandran, Hannah R. Vasanthi, G. V. Rajamanickam
    Abstract:

    Altered Membrane integrity has been suggested as a major factor in the development of cellular injury during myocardial necrosis. The present study was designed to investigate the Effect of diosgenin on lysosomal hydrolases, Membrane-bound enzymes, and electrolytes during isoproterenol (ISO)-induced myocardial necrosis in rats. Animals were pretreated with DIOS (80 mg/kg) for a period of 35 days. Myocardial infarction was experimentally induced with ISO (85 mg/kg) twice at 24 h interval. Experimental myocardial infarction was evidenced with marked elevation of creatine kinase-MB (CK-MB) in serum with concomitant increase in lipid peroxidation (plasma thiobarbituric acid reactive substances (TBARS) and hydroperoxides (HP)). Activity of lysosomal hydrolases (beta-glucuronidase, beta-N-acetyl glucosaminidase, beta-D-galactosidase, cathepsin D, and acid phosphatase) was found to be increased in serum and heart tissue of ISO-alone treated animals. DIOS (80 mg/kg) pretreated groups showed significant decrease in CK-MB, lipid peroxidation, and lysosomal hydrolases activity. The Membrane-bound enzymes such as Ca2+-ATPase and Mg2+-ATPase activity was increased and Na+/K+-ATPase activity was decreased in the heart tissues of ISO-alone treated animals. These enzyme alterations lead to the change in the electrolytes content such as sodium, potassium, and calcium in the heart tissue. However, DIOS (80 mg/kg) pretreatment reversed the Membrane-bound enzymes activity and thereby maintained the normal electrolyte concentration. These results suggest the protective action of diosgenin in ISO-induced myocardial infarction. The salubrious Effect observed in this study might be due to the antioxidant and Membrane Stabilizing potential of diosgenin.