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Matthew S. Yorek - One of the best experts on this subject based on the ideXlab platform.
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effect of dietary content of Menhaden oil with or without salsalate on neuropathic endpoints in high fat fed low dose streptozotocin treated sprague dawley rats
Experimental Diabetes Research, 2018Co-Authors: Eric P. Davidson, Matthew S. Yorek, Lawrence J. Coppey, Hanna Shevalye, Alexander ObrosovAbstract:In this study, we wanted to extend our investigation of the efficacy of fish oil with or without salsalate on vascular and neural complications using a type 2 diabetic rat model. Four weeks after the onset of hyperglycemia, diabetic rats were treated via the diet with 3 different amounts of Menhaden oil with or without salsalate for 12 weeks. Afterwards, vascular reactivity of epineurial arterioles and neuropathy-related endpoints were examined. The addition of salsalate to high-fat diets enriched with 10% or 25% kcal of Menhaden oil protected vascular reactivity to acetylcholine and calcium gene-related peptide, motor and sensory nerve conduction velocity, thermal nociception, intraepidermal nerve fiber density, and cornea sensitivity to a greater extent than 10% or 25% Menhaden oil alone. Vascular and neural function was maximally protected with diet containing 45% kcal as Menhaden oil, and adding salsalate did not provide any additional benefit. Salsalate alone in the high-fat diet of diabetic rats provided minimal protection/improvement of vascular and neural dysfunction. These studies imply that dietary salsalate in combination with lower amounts of Menhaden oil can provide greater benefit toward diabetes-induced vascular and neural impairment than Menhaden oil alone.
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effect of treatment with salsalate Menhaden oil combination of salsalate and Menhaden oil or resolvin d1 of c57bl 6j type 1 diabetic mouse on neuropathic endpoints
Journal of Nutrition and Metabolism, 2016Co-Authors: Hanna Shevalye, Lawrence J. Coppey, Alexander Obrosov, Randy H. Kardon, Matthew S. YorekAbstract:Aims. In this study a streptozotocin induced type 1 diabetes mouse model was used to assess the effectiveness of salsalate, Menhaden oil, the combination of salsalate and Menhaden oil, or resolvin D1 on neuropathic endpoints. Materials and Methods. Changes in body weight, blood glucose, serum markers for triglycerides, free fatty acids, cholesterol, and resolvin D1, motor and sensory nerve conduction velocities and thermal sensitivity were assessed, as well as performing in vivo confocal microscopy of subepithelial corneal nerves and immunohistochemistry of nerves in the cornea and foot pad. Results. Diabetic animals failed to gain weight and had elevated blood glucose levels. Diabetic mice had slowed nerve conduction velocity, reduced innervation of the foot pad and cornea subepithelial and epithelial layers, and reduced thermal sensitivity. Monotherapy treatment with salsalate, Menhaden oil, and resolvin D1 reduced the pathological signs of diabetic neuropathy. The combination of salsalate and Menhaden oil also reduced signs of pathology and generated elevated plasma levels of resolvin D1 compared to other groups. Conclusions. Additional studies are needed to determine whether the combination of salsalate and Menhaden oil may be more efficacious than monotherapy alone for the treatment of diabetic peripheral neuropathy.
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effect of enriching the diet with Menhaden oil or daily treatment with resolvin d1 on neuropathy in a mouse model of type 2 diabetes
Journal of Neurophysiology, 2015Co-Authors: Hanna Shevalye, Matthew S. Yorek, Lawrence J. Coppey, Amey Holmes, Matthew M Harper, Randy H. KardonAbstract:The purpose of this study was to determine the effect of supplementing the diet of a mouse model of type 2 diabetes with Menhaden (fish) oil or daily treatment with resolvin D1 on diabetic neuropathy. The end points evaluated included motor and sensory nerve conduction velocity, thermal sensitivity, innervation of sensory nerves in the cornea and skin, and the retinal ganglion cell complex thickness. Menhaden oil is a natural source for n-3 polyunsaturated fatty acids, which have been shown to have beneficial effects in other diseases. Resolvin D1 is a metabolite of docosahexaenoic acid and is known to have anti-inflammatory and neuroprotective properties. To model type 2 diabetes, mice were fed a high-fat diet for 8 wk followed by a low dosage of streptozotocin. After 8 wk of hyperglycemia, mice in experimental groups were treated for 6 wk with Menhaden oil in the diet or daily injections of 1 ng/g body wt resolvin D1. Our findings show that Menhaden oil or resolvin D1 did not improve elevated blood glucose, HbA1C, or glucose utilization. Untreated diabetic mice were thermal hypoalgesic, had reduced motor and sensory nerve conduction velocities, had decreased innervation of the cornea and skin, and had thinner retinal ganglion cell complex. These end points were significantly improved with Menhaden oil or resolvin D1 treatment. Exogenously, resolvin D1 stimulated neurite outgrowth from primary cultures of dorsal root ganglion neurons from normal mice. These studies suggest that n-3 polyunsaturated fatty acids derived from fish oil could be an effective treatment for diabetic neuropathy.
Lawrence J. Coppey - One of the best experts on this subject based on the ideXlab platform.
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Effect of Dietary Content of Menhaden Oil with or without Salsalate on Neuropathic Endpoints in High-Fat-Fed/Low-Dose Streptozotocin-Treated Sprague Dawley Rats.
Journal of diabetes research, 2018Co-Authors: Eric P. Davidson, Lawrence J. Coppey, Hanna Shevalye, Alexander ObrosovAbstract:In this study, we wanted to extend our investigation of the efficacy of fish oil with or without salsalate on vascular and neural complications using a type 2 diabetic rat model. Four weeks after the onset of hyperglycemia, diabetic rats were treated via the diet with 3 different amounts of Menhaden oil with or without salsalate for 12 weeks. Afterwards, vascular reactivity of epineurial arterioles and neuropathy-related endpoints were examined. The addition of salsalate to high-fat diets enriched with 10% or 25% kcal of Menhaden oil protected vascular reactivity to acetylcholine and calcium gene-related peptide, motor and sensory nerve conduction velocity, thermal nociception, intraepidermal nerve fiber density, and cornea sensitivity to a greater extent than 10% or 25% Menhaden oil alone. Vascular and neural function was maximally protected with diet containing 45% kcal as Menhaden oil, and adding salsalate did not provide any additional benefit. Salsalate alone in the high-fat diet of diabetic rats provided minimal protection/improvement of vascular and neural dysfunction. These studies imply that dietary salsalate in combination with lower amounts of Menhaden oil can provide greater benefit toward diabetes-induced vascular and neural impairment than Menhaden oil alone.
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effect of dietary content of Menhaden oil with or without salsalate on neuropathic endpoints in high fat fed low dose streptozotocin treated sprague dawley rats
Experimental Diabetes Research, 2018Co-Authors: Eric P. Davidson, Matthew S. Yorek, Lawrence J. Coppey, Hanna Shevalye, Alexander ObrosovAbstract:In this study, we wanted to extend our investigation of the efficacy of fish oil with or without salsalate on vascular and neural complications using a type 2 diabetic rat model. Four weeks after the onset of hyperglycemia, diabetic rats were treated via the diet with 3 different amounts of Menhaden oil with or without salsalate for 12 weeks. Afterwards, vascular reactivity of epineurial arterioles and neuropathy-related endpoints were examined. The addition of salsalate to high-fat diets enriched with 10% or 25% kcal of Menhaden oil protected vascular reactivity to acetylcholine and calcium gene-related peptide, motor and sensory nerve conduction velocity, thermal nociception, intraepidermal nerve fiber density, and cornea sensitivity to a greater extent than 10% or 25% Menhaden oil alone. Vascular and neural function was maximally protected with diet containing 45% kcal as Menhaden oil, and adding salsalate did not provide any additional benefit. Salsalate alone in the high-fat diet of diabetic rats provided minimal protection/improvement of vascular and neural dysfunction. These studies imply that dietary salsalate in combination with lower amounts of Menhaden oil can provide greater benefit toward diabetes-induced vascular and neural impairment than Menhaden oil alone.
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effect of treatment with salsalate Menhaden oil combination of salsalate and Menhaden oil or resolvin d1 of c57bl 6j type 1 diabetic mouse on neuropathic endpoints
Journal of Nutrition and Metabolism, 2016Co-Authors: Hanna Shevalye, Lawrence J. Coppey, Alexander Obrosov, Randy H. Kardon, Matthew S. YorekAbstract:Aims. In this study a streptozotocin induced type 1 diabetes mouse model was used to assess the effectiveness of salsalate, Menhaden oil, the combination of salsalate and Menhaden oil, or resolvin D1 on neuropathic endpoints. Materials and Methods. Changes in body weight, blood glucose, serum markers for triglycerides, free fatty acids, cholesterol, and resolvin D1, motor and sensory nerve conduction velocities and thermal sensitivity were assessed, as well as performing in vivo confocal microscopy of subepithelial corneal nerves and immunohistochemistry of nerves in the cornea and foot pad. Results. Diabetic animals failed to gain weight and had elevated blood glucose levels. Diabetic mice had slowed nerve conduction velocity, reduced innervation of the foot pad and cornea subepithelial and epithelial layers, and reduced thermal sensitivity. Monotherapy treatment with salsalate, Menhaden oil, and resolvin D1 reduced the pathological signs of diabetic neuropathy. The combination of salsalate and Menhaden oil also reduced signs of pathology and generated elevated plasma levels of resolvin D1 compared to other groups. Conclusions. Additional studies are needed to determine whether the combination of salsalate and Menhaden oil may be more efficacious than monotherapy alone for the treatment of diabetic peripheral neuropathy.
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effect of enriching the diet with Menhaden oil or daily treatment with resolvin d1 on neuropathy in a mouse model of type 2 diabetes
Journal of Neurophysiology, 2015Co-Authors: Hanna Shevalye, Matthew S. Yorek, Lawrence J. Coppey, Amey Holmes, Matthew M Harper, Randy H. KardonAbstract:The purpose of this study was to determine the effect of supplementing the diet of a mouse model of type 2 diabetes with Menhaden (fish) oil or daily treatment with resolvin D1 on diabetic neuropathy. The end points evaluated included motor and sensory nerve conduction velocity, thermal sensitivity, innervation of sensory nerves in the cornea and skin, and the retinal ganglion cell complex thickness. Menhaden oil is a natural source for n-3 polyunsaturated fatty acids, which have been shown to have beneficial effects in other diseases. Resolvin D1 is a metabolite of docosahexaenoic acid and is known to have anti-inflammatory and neuroprotective properties. To model type 2 diabetes, mice were fed a high-fat diet for 8 wk followed by a low dosage of streptozotocin. After 8 wk of hyperglycemia, mice in experimental groups were treated for 6 wk with Menhaden oil in the diet or daily injections of 1 ng/g body wt resolvin D1. Our findings show that Menhaden oil or resolvin D1 did not improve elevated blood glucose, HbA1C, or glucose utilization. Untreated diabetic mice were thermal hypoalgesic, had reduced motor and sensory nerve conduction velocities, had decreased innervation of the cornea and skin, and had thinner retinal ganglion cell complex. These end points were significantly improved with Menhaden oil or resolvin D1 treatment. Exogenously, resolvin D1 stimulated neurite outgrowth from primary cultures of dorsal root ganglion neurons from normal mice. These studies suggest that n-3 polyunsaturated fatty acids derived from fish oil could be an effective treatment for diabetic neuropathy.
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Enriching the diet with Menhaden oil improves peripheral neuropathy in streptozotocin-induced type 1 diabetic rats
Journal of neurophysiology, 2014Co-Authors: Lawrence J. Coppey, Eric P. Davidson, Alexander ObrosovAbstract:The purpose of this study was to determine the effect of supplementing the diet of type 1 diabetic rats with Menhaden oil on diabetic neuropathy. Menhaden oil is a natural source for n-3 fatty acids, which have been shown to have beneficial effects in cardiovascular disease and other morbidities. Streptozotocin-induced diabetic rats were used to examine the influence of supplementing their diet with 25% Menhaden oil on diabetic neuropathy. Both prevention and intervention protocols were used. Endpoints included motor and sensory nerve conduction velocity, thermal and mechanical sensitivity, and innervation and sensitivity of the cornea and hindpaw. Diabetic neuropathy as evaluated by the stated endpoints was found to be progressive. Menhaden oil did not improve elevated HbA1C levels or serum lipid levels. Diabetic rats at 16-wk duration were thermal hypoalgesic and had reduced motor and sensory nerve conduction velocities, and innervation and sensitivity of the cornea and skin were impaired. These endpoints were significantly improved with Menhaden oil treatment following the prevention or intervention protocol. We found that supplementing the diet of type 1 diabetic rats with Menhaden oil improved a variety of endpoints associated with diabetic neuropathy. These results suggest that enriching the diet with n-3 fatty acids may be a good treatment strategy for diabetic neuropathy.
Alexander Obrosov - One of the best experts on this subject based on the ideXlab platform.
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Effect of Dietary Content of Menhaden Oil with or without Salsalate on Neuropathic Endpoints in High-Fat-Fed/Low-Dose Streptozotocin-Treated Sprague Dawley Rats.
Journal of diabetes research, 2018Co-Authors: Eric P. Davidson, Lawrence J. Coppey, Hanna Shevalye, Alexander ObrosovAbstract:In this study, we wanted to extend our investigation of the efficacy of fish oil with or without salsalate on vascular and neural complications using a type 2 diabetic rat model. Four weeks after the onset of hyperglycemia, diabetic rats were treated via the diet with 3 different amounts of Menhaden oil with or without salsalate for 12 weeks. Afterwards, vascular reactivity of epineurial arterioles and neuropathy-related endpoints were examined. The addition of salsalate to high-fat diets enriched with 10% or 25% kcal of Menhaden oil protected vascular reactivity to acetylcholine and calcium gene-related peptide, motor and sensory nerve conduction velocity, thermal nociception, intraepidermal nerve fiber density, and cornea sensitivity to a greater extent than 10% or 25% Menhaden oil alone. Vascular and neural function was maximally protected with diet containing 45% kcal as Menhaden oil, and adding salsalate did not provide any additional benefit. Salsalate alone in the high-fat diet of diabetic rats provided minimal protection/improvement of vascular and neural dysfunction. These studies imply that dietary salsalate in combination with lower amounts of Menhaden oil can provide greater benefit toward diabetes-induced vascular and neural impairment than Menhaden oil alone.
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effect of dietary content of Menhaden oil with or without salsalate on neuropathic endpoints in high fat fed low dose streptozotocin treated sprague dawley rats
Experimental Diabetes Research, 2018Co-Authors: Eric P. Davidson, Matthew S. Yorek, Lawrence J. Coppey, Hanna Shevalye, Alexander ObrosovAbstract:In this study, we wanted to extend our investigation of the efficacy of fish oil with or without salsalate on vascular and neural complications using a type 2 diabetic rat model. Four weeks after the onset of hyperglycemia, diabetic rats were treated via the diet with 3 different amounts of Menhaden oil with or without salsalate for 12 weeks. Afterwards, vascular reactivity of epineurial arterioles and neuropathy-related endpoints were examined. The addition of salsalate to high-fat diets enriched with 10% or 25% kcal of Menhaden oil protected vascular reactivity to acetylcholine and calcium gene-related peptide, motor and sensory nerve conduction velocity, thermal nociception, intraepidermal nerve fiber density, and cornea sensitivity to a greater extent than 10% or 25% Menhaden oil alone. Vascular and neural function was maximally protected with diet containing 45% kcal as Menhaden oil, and adding salsalate did not provide any additional benefit. Salsalate alone in the high-fat diet of diabetic rats provided minimal protection/improvement of vascular and neural dysfunction. These studies imply that dietary salsalate in combination with lower amounts of Menhaden oil can provide greater benefit toward diabetes-induced vascular and neural impairment than Menhaden oil alone.
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effect of treatment with salsalate Menhaden oil combination of salsalate and Menhaden oil or resolvin d1 of c57bl 6j type 1 diabetic mouse on neuropathic endpoints
Journal of Nutrition and Metabolism, 2016Co-Authors: Hanna Shevalye, Lawrence J. Coppey, Alexander Obrosov, Randy H. Kardon, Matthew S. YorekAbstract:Aims. In this study a streptozotocin induced type 1 diabetes mouse model was used to assess the effectiveness of salsalate, Menhaden oil, the combination of salsalate and Menhaden oil, or resolvin D1 on neuropathic endpoints. Materials and Methods. Changes in body weight, blood glucose, serum markers for triglycerides, free fatty acids, cholesterol, and resolvin D1, motor and sensory nerve conduction velocities and thermal sensitivity were assessed, as well as performing in vivo confocal microscopy of subepithelial corneal nerves and immunohistochemistry of nerves in the cornea and foot pad. Results. Diabetic animals failed to gain weight and had elevated blood glucose levels. Diabetic mice had slowed nerve conduction velocity, reduced innervation of the foot pad and cornea subepithelial and epithelial layers, and reduced thermal sensitivity. Monotherapy treatment with salsalate, Menhaden oil, and resolvin D1 reduced the pathological signs of diabetic neuropathy. The combination of salsalate and Menhaden oil also reduced signs of pathology and generated elevated plasma levels of resolvin D1 compared to other groups. Conclusions. Additional studies are needed to determine whether the combination of salsalate and Menhaden oil may be more efficacious than monotherapy alone for the treatment of diabetic peripheral neuropathy.
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Enriching the diet with Menhaden oil improves peripheral neuropathy in streptozotocin-induced type 1 diabetic rats
Journal of neurophysiology, 2014Co-Authors: Lawrence J. Coppey, Eric P. Davidson, Alexander ObrosovAbstract:The purpose of this study was to determine the effect of supplementing the diet of type 1 diabetic rats with Menhaden oil on diabetic neuropathy. Menhaden oil is a natural source for n-3 fatty acids, which have been shown to have beneficial effects in cardiovascular disease and other morbidities. Streptozotocin-induced diabetic rats were used to examine the influence of supplementing their diet with 25% Menhaden oil on diabetic neuropathy. Both prevention and intervention protocols were used. Endpoints included motor and sensory nerve conduction velocity, thermal and mechanical sensitivity, and innervation and sensitivity of the cornea and hindpaw. Diabetic neuropathy as evaluated by the stated endpoints was found to be progressive. Menhaden oil did not improve elevated HbA1C levels or serum lipid levels. Diabetic rats at 16-wk duration were thermal hypoalgesic and had reduced motor and sensory nerve conduction velocities, and innervation and sensitivity of the cornea and skin were impaired. These endpoints were significantly improved with Menhaden oil treatment following the prevention or intervention protocol. We found that supplementing the diet of type 1 diabetic rats with Menhaden oil improved a variety of endpoints associated with diabetic neuropathy. These results suggest that enriching the diet with n-3 fatty acids may be a good treatment strategy for diabetic neuropathy.
Eric P. Davidson - One of the best experts on this subject based on the ideXlab platform.
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Effect of Dietary Content of Menhaden Oil with or without Salsalate on Neuropathic Endpoints in High-Fat-Fed/Low-Dose Streptozotocin-Treated Sprague Dawley Rats.
Journal of diabetes research, 2018Co-Authors: Eric P. Davidson, Lawrence J. Coppey, Hanna Shevalye, Alexander ObrosovAbstract:In this study, we wanted to extend our investigation of the efficacy of fish oil with or without salsalate on vascular and neural complications using a type 2 diabetic rat model. Four weeks after the onset of hyperglycemia, diabetic rats were treated via the diet with 3 different amounts of Menhaden oil with or without salsalate for 12 weeks. Afterwards, vascular reactivity of epineurial arterioles and neuropathy-related endpoints were examined. The addition of salsalate to high-fat diets enriched with 10% or 25% kcal of Menhaden oil protected vascular reactivity to acetylcholine and calcium gene-related peptide, motor and sensory nerve conduction velocity, thermal nociception, intraepidermal nerve fiber density, and cornea sensitivity to a greater extent than 10% or 25% Menhaden oil alone. Vascular and neural function was maximally protected with diet containing 45% kcal as Menhaden oil, and adding salsalate did not provide any additional benefit. Salsalate alone in the high-fat diet of diabetic rats provided minimal protection/improvement of vascular and neural dysfunction. These studies imply that dietary salsalate in combination with lower amounts of Menhaden oil can provide greater benefit toward diabetes-induced vascular and neural impairment than Menhaden oil alone.
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effect of dietary content of Menhaden oil with or without salsalate on neuropathic endpoints in high fat fed low dose streptozotocin treated sprague dawley rats
Experimental Diabetes Research, 2018Co-Authors: Eric P. Davidson, Matthew S. Yorek, Lawrence J. Coppey, Hanna Shevalye, Alexander ObrosovAbstract:In this study, we wanted to extend our investigation of the efficacy of fish oil with or without salsalate on vascular and neural complications using a type 2 diabetic rat model. Four weeks after the onset of hyperglycemia, diabetic rats were treated via the diet with 3 different amounts of Menhaden oil with or without salsalate for 12 weeks. Afterwards, vascular reactivity of epineurial arterioles and neuropathy-related endpoints were examined. The addition of salsalate to high-fat diets enriched with 10% or 25% kcal of Menhaden oil protected vascular reactivity to acetylcholine and calcium gene-related peptide, motor and sensory nerve conduction velocity, thermal nociception, intraepidermal nerve fiber density, and cornea sensitivity to a greater extent than 10% or 25% Menhaden oil alone. Vascular and neural function was maximally protected with diet containing 45% kcal as Menhaden oil, and adding salsalate did not provide any additional benefit. Salsalate alone in the high-fat diet of diabetic rats provided minimal protection/improvement of vascular and neural dysfunction. These studies imply that dietary salsalate in combination with lower amounts of Menhaden oil can provide greater benefit toward diabetes-induced vascular and neural impairment than Menhaden oil alone.
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Enriching the diet with Menhaden oil improves peripheral neuropathy in streptozotocin-induced type 1 diabetic rats
Journal of neurophysiology, 2014Co-Authors: Lawrence J. Coppey, Eric P. Davidson, Alexander ObrosovAbstract:The purpose of this study was to determine the effect of supplementing the diet of type 1 diabetic rats with Menhaden oil on diabetic neuropathy. Menhaden oil is a natural source for n-3 fatty acids, which have been shown to have beneficial effects in cardiovascular disease and other morbidities. Streptozotocin-induced diabetic rats were used to examine the influence of supplementing their diet with 25% Menhaden oil on diabetic neuropathy. Both prevention and intervention protocols were used. Endpoints included motor and sensory nerve conduction velocity, thermal and mechanical sensitivity, and innervation and sensitivity of the cornea and hindpaw. Diabetic neuropathy as evaluated by the stated endpoints was found to be progressive. Menhaden oil did not improve elevated HbA1C levels or serum lipid levels. Diabetic rats at 16-wk duration were thermal hypoalgesic and had reduced motor and sensory nerve conduction velocities, and innervation and sensitivity of the cornea and skin were impaired. These endpoints were significantly improved with Menhaden oil treatment following the prevention or intervention protocol. We found that supplementing the diet of type 1 diabetic rats with Menhaden oil improved a variety of endpoints associated with diabetic neuropathy. These results suggest that enriching the diet with n-3 fatty acids may be a good treatment strategy for diabetic neuropathy.
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Partial Replacement with Menhaden Oil Improves Peripheral Neuropathy in High-Fat-Fed Low-Dose Streptozotocin Type 2 Diabetic Rat
Journal of nutrition and metabolism, 2012Co-Authors: Lawrence J. Coppey, Amey Holmes, Eric P. DavidsonAbstract:Aims. To determine the effect of partial replacement of a high-fat diet with Menhaden oil on diabetic neuropathy in an animal model of type 2 diabetes. Materials and Methods. High-fat/low-dose streptozotocin diabetic rats were used to examine the influence of replacing 50% of the source of the high-fat diet (lard) with Menhaden oil, a natural source of n-3 fatty acids, on diabetic neuropathy. Endpoints included analyses of glucose tolerance, fatty liver disease, serum and liver fatty acid composition, serum lipid and adiponectin levels, motor and sensory nerve conduction velocity, thermal sensitivity and innervation of the hindpaw. Results. Diabetic rats were insulin resistant and Menhaden oil did not improve whole animal glucose utilization. Menhaden oil did not improve elevated HbA1C levels or serum lipid levels but serum levels of adiponectin were significantly increased and hepatic steatosis was significantly improved. Diabetic rats were thermal hypoalgesic, had reduced motor and sensory nerve conduction velocities and intraepidermal nerve fiber profiles were decreased in the hindpaw and these endpoints were significantly improved with Menhaden oil. Conclusions. We found that enrichment of a high-fat diet with Menhaden oil improved a number of endpoints associated with diabetic neuropathy.
Hanna Shevalye - One of the best experts on this subject based on the ideXlab platform.
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effect of dietary content of Menhaden oil with or without salsalate on neuropathic endpoints in high fat fed low dose streptozotocin treated sprague dawley rats
Experimental Diabetes Research, 2018Co-Authors: Eric P. Davidson, Matthew S. Yorek, Lawrence J. Coppey, Hanna Shevalye, Alexander ObrosovAbstract:In this study, we wanted to extend our investigation of the efficacy of fish oil with or without salsalate on vascular and neural complications using a type 2 diabetic rat model. Four weeks after the onset of hyperglycemia, diabetic rats were treated via the diet with 3 different amounts of Menhaden oil with or without salsalate for 12 weeks. Afterwards, vascular reactivity of epineurial arterioles and neuropathy-related endpoints were examined. The addition of salsalate to high-fat diets enriched with 10% or 25% kcal of Menhaden oil protected vascular reactivity to acetylcholine and calcium gene-related peptide, motor and sensory nerve conduction velocity, thermal nociception, intraepidermal nerve fiber density, and cornea sensitivity to a greater extent than 10% or 25% Menhaden oil alone. Vascular and neural function was maximally protected with diet containing 45% kcal as Menhaden oil, and adding salsalate did not provide any additional benefit. Salsalate alone in the high-fat diet of diabetic rats provided minimal protection/improvement of vascular and neural dysfunction. These studies imply that dietary salsalate in combination with lower amounts of Menhaden oil can provide greater benefit toward diabetes-induced vascular and neural impairment than Menhaden oil alone.
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Effect of Dietary Content of Menhaden Oil with or without Salsalate on Neuropathic Endpoints in High-Fat-Fed/Low-Dose Streptozotocin-Treated Sprague Dawley Rats.
Journal of diabetes research, 2018Co-Authors: Eric P. Davidson, Lawrence J. Coppey, Hanna Shevalye, Alexander ObrosovAbstract:In this study, we wanted to extend our investigation of the efficacy of fish oil with or without salsalate on vascular and neural complications using a type 2 diabetic rat model. Four weeks after the onset of hyperglycemia, diabetic rats were treated via the diet with 3 different amounts of Menhaden oil with or without salsalate for 12 weeks. Afterwards, vascular reactivity of epineurial arterioles and neuropathy-related endpoints were examined. The addition of salsalate to high-fat diets enriched with 10% or 25% kcal of Menhaden oil protected vascular reactivity to acetylcholine and calcium gene-related peptide, motor and sensory nerve conduction velocity, thermal nociception, intraepidermal nerve fiber density, and cornea sensitivity to a greater extent than 10% or 25% Menhaden oil alone. Vascular and neural function was maximally protected with diet containing 45% kcal as Menhaden oil, and adding salsalate did not provide any additional benefit. Salsalate alone in the high-fat diet of diabetic rats provided minimal protection/improvement of vascular and neural dysfunction. These studies imply that dietary salsalate in combination with lower amounts of Menhaden oil can provide greater benefit toward diabetes-induced vascular and neural impairment than Menhaden oil alone.
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effect of treatment with salsalate Menhaden oil combination of salsalate and Menhaden oil or resolvin d1 of c57bl 6j type 1 diabetic mouse on neuropathic endpoints
Journal of Nutrition and Metabolism, 2016Co-Authors: Hanna Shevalye, Lawrence J. Coppey, Alexander Obrosov, Randy H. Kardon, Matthew S. YorekAbstract:Aims. In this study a streptozotocin induced type 1 diabetes mouse model was used to assess the effectiveness of salsalate, Menhaden oil, the combination of salsalate and Menhaden oil, or resolvin D1 on neuropathic endpoints. Materials and Methods. Changes in body weight, blood glucose, serum markers for triglycerides, free fatty acids, cholesterol, and resolvin D1, motor and sensory nerve conduction velocities and thermal sensitivity were assessed, as well as performing in vivo confocal microscopy of subepithelial corneal nerves and immunohistochemistry of nerves in the cornea and foot pad. Results. Diabetic animals failed to gain weight and had elevated blood glucose levels. Diabetic mice had slowed nerve conduction velocity, reduced innervation of the foot pad and cornea subepithelial and epithelial layers, and reduced thermal sensitivity. Monotherapy treatment with salsalate, Menhaden oil, and resolvin D1 reduced the pathological signs of diabetic neuropathy. The combination of salsalate and Menhaden oil also reduced signs of pathology and generated elevated plasma levels of resolvin D1 compared to other groups. Conclusions. Additional studies are needed to determine whether the combination of salsalate and Menhaden oil may be more efficacious than monotherapy alone for the treatment of diabetic peripheral neuropathy.
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effect of enriching the diet with Menhaden oil or daily treatment with resolvin d1 on neuropathy in a mouse model of type 2 diabetes
Journal of Neurophysiology, 2015Co-Authors: Hanna Shevalye, Matthew S. Yorek, Lawrence J. Coppey, Amey Holmes, Matthew M Harper, Randy H. KardonAbstract:The purpose of this study was to determine the effect of supplementing the diet of a mouse model of type 2 diabetes with Menhaden (fish) oil or daily treatment with resolvin D1 on diabetic neuropathy. The end points evaluated included motor and sensory nerve conduction velocity, thermal sensitivity, innervation of sensory nerves in the cornea and skin, and the retinal ganglion cell complex thickness. Menhaden oil is a natural source for n-3 polyunsaturated fatty acids, which have been shown to have beneficial effects in other diseases. Resolvin D1 is a metabolite of docosahexaenoic acid and is known to have anti-inflammatory and neuroprotective properties. To model type 2 diabetes, mice were fed a high-fat diet for 8 wk followed by a low dosage of streptozotocin. After 8 wk of hyperglycemia, mice in experimental groups were treated for 6 wk with Menhaden oil in the diet or daily injections of 1 ng/g body wt resolvin D1. Our findings show that Menhaden oil or resolvin D1 did not improve elevated blood glucose, HbA1C, or glucose utilization. Untreated diabetic mice were thermal hypoalgesic, had reduced motor and sensory nerve conduction velocities, had decreased innervation of the cornea and skin, and had thinner retinal ganglion cell complex. These end points were significantly improved with Menhaden oil or resolvin D1 treatment. Exogenously, resolvin D1 stimulated neurite outgrowth from primary cultures of dorsal root ganglion neurons from normal mice. These studies suggest that n-3 polyunsaturated fatty acids derived from fish oil could be an effective treatment for diabetic neuropathy.