The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Emilio Agostoni - One of the best experts on this subject based on the ideXlab platform.
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pleural Mesothelium lubrication after hyaluronidase neuraminidase or pronase treatment
Respiratory Physiology & Neurobiology, 2013Co-Authors: Chiara Sironi, Francesca Bodega, Cristina Porta, Emilio AgostoniAbstract:Abstract Coefficient of kinetic friction ( μ ) of pleural Mesothelium has been found to increase markedly after mesothelial blotting and rewetting. This increase disappeared after addition of a solution with hyaluronan or sialomucin, though previous morphological studies showed that only sialomucin occurs in mesothelial glycocalyx. In this research we investigated whether μ of rabbit pleural Mesothelium increased after hyaluronidase, neuraminidase or pronase treatment. Hyaluronidase and neuraminidase did not increase μ , though neuraminidase cleaved sialic acid from mesothelial glycocalyx of diaphragm specimens, and removed hystochemical stain of sialic acid from glycocalyx. Sialomucin treated with neuraminidase lowered μ of blotted Mesothelium, though less than untreated sialomucin; this feature plus lubrication provided by other molecules could explain why μ did not increase after neuraminidase. Short pronase treatment (in order to affect only glycocalyx proteins) increased μ ; this increase was removed by hyaluronan or sialomucin. After pronase treatment μ decreased with increase in sliding velocity, indicating a regime of mixed lubrication, as in blotted Mesothelium.
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β2-Adrenergic receptors and G-protein-coupled receptor kinase 2 in rabbit pleural Mesothelium.
Respiratory physiology & neurobiology, 2010Co-Authors: Chiara Sironi, Francesca Bodega, Luciano Zocchi, Cristina Porta, Marta Armilli, Emilio AgostoniAbstract:Former studies on net rate of liquid absorption from small Ringer or 1% albumin-Ringer hydrothoraces in rabbits indicated that Na+ transport and solute-coupled liquid absorption by Mesothelium is increased by pleural liquid dilution, and stimulation of β2-adrenoreceptors (β2AR). In this research we tried to provide molecular evidence for β2AR in visceral and parietal Mesothelium of rabbit pleura. Moreover, because prolonged stimulation of β2AR may lead to desensitization mediated by G-protein-coupled receptor kinase 2 (GRK2), we also checked whether GRK2 is expressed in pleural Mesothelium. To this end we performed immunoblot assays on total protein extracts from scraped visceral and parietal Mesothelium, and from cultured pleural mesothelial cells of rabbits. All three samples showed β2AR and GRK2 specific bands.
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na glucose cotransporter is also expressed in Mesothelium of species with thick visceral pleura
Respiratory Physiology & Neurobiology, 2008Co-Authors: Chiara Sironi, Francesca Odega, Ario Monaco, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Molecular evidence for Na+–glucose cotransporter (SGLT1) in rabbit pleural Mesothelium has been recently provided, confirming earlier functional findings on solute-coupled liquid absorption from rabbit pleural space. In this research we checked whether SGLT1 is also expressed in pleural Mesothelium of species with thick visceral pleura, which receives blood from systemic circulation, but drains it into pulmonary veins. To this end immunoblot assays were performed on total protein extract of scraped visceral and parietal Mesothelium of lambs and adult sheep, and of a human mesothelial cell line. All of them showed SGLT1 specific bands. Moreover, confocal immunofluorescence images of lamb pleural Mesothelium showed that SGLT1 is located in apical membrane. Therefore, a solute-coupled liquid absorption should also occur from pleural space of species with thick visceral pleura. Because of this protein-free liquid entering interstitium between visceral Mesothelium and capillaries, inherent Starling forces should be different than hitherto considered, and visceral pleura capillaries could absorb liquid even in these species.
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Na+-glucose cotransporter is also expressed in Mesothelium of species with thick visceral pleura.
Respiratory physiology & neurobiology, 2008Co-Authors: Chiara Sironi, Francesca Bodega, Ario Monaco, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Molecular evidence for Na+–glucose cotransporter (SGLT1) in rabbit pleural Mesothelium has been recently provided, confirming earlier functional findings on solute-coupled liquid absorption from rabbit pleural space. In this research we checked whether SGLT1 is also expressed in pleural Mesothelium of species with thick visceral pleura, which receives blood from systemic circulation, but drains it into pulmonary veins. To this end immunoblot assays were performed on total protein extract of scraped visceral and parietal Mesothelium of lambs and adult sheep, and of a human mesothelial cell line. All of them showed SGLT1 specific bands. Moreover, confocal immunofluorescence images of lamb pleural Mesothelium showed that SGLT1 is located in apical membrane. Therefore, a solute-coupled liquid absorption should also occur from pleural space of species with thick visceral pleura. Because of this protein-free liquid entering interstitium between visceral Mesothelium and capillaries, inherent Starling forces should be different than hitherto considered, and visceral pleura capillaries could absorb liquid even in these species.
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expression of na glucose cotransporter sglt1 in visceral and parietal Mesothelium of rabbit pleura
Respiratory Physiology & Neurobiology, 2007Co-Authors: Chiara Sironi, Francesca Odega, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Indirect evidence for a solute-coupled liquid absorption from rabbit pleural space indicated that it should be caused by a Na + /H + –Cl − /HCO 3 − double exchanger and a Na + –glucose cotransporter [Agostoni, E., Zocchi, L., 1998. Mechanical coupling and liquid exchanges in the pleural space. In: Antony, V.B. (Ed.), Clinics in Chest Medicine: Diseases of the Pleura, vol. 19. Saunders, Philadelphia, pp. 241–260]. In this research we tried to obtain molecular evidence for Na + –glucose cotransporter (SGLT1) in visceral and parietal Mesothelium of rabbit pleura. To this end we performed immunoblot assays on total protein extracts of scraped visceral or parietal Mesothelium of rabbits. These showed two bands: one at 72 kDa (m.w. of SGLT1), and one at 55 kDa (which should also provide Na + –glucose cotransport). Both bands disappeared in assays in which SGLT1 antibody was preadsorbed with specific antigen. Molecular evidence for Na + /K + ATPase (α1 subunit) was also provided. Immunoblot assays for SGLT1 on cultured mesothelial cells of rabbit pleura showed a band at 72 kDa, and in some cases also at 55 kDa, irrespectively of treatment with a differentiating agent. Solute-coupled liquid absorption hinders liquid filtration through parietal Mesothelium caused by Starling forces, and favours liquid absorption through visceral Mesothelium caused by these forces.
Chiara Sironi - One of the best experts on this subject based on the ideXlab platform.
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pleural Mesothelium lubrication after hyaluronidase neuraminidase or pronase treatment
Respiratory Physiology & Neurobiology, 2013Co-Authors: Chiara Sironi, Francesca Bodega, Cristina Porta, Emilio AgostoniAbstract:Abstract Coefficient of kinetic friction ( μ ) of pleural Mesothelium has been found to increase markedly after mesothelial blotting and rewetting. This increase disappeared after addition of a solution with hyaluronan or sialomucin, though previous morphological studies showed that only sialomucin occurs in mesothelial glycocalyx. In this research we investigated whether μ of rabbit pleural Mesothelium increased after hyaluronidase, neuraminidase or pronase treatment. Hyaluronidase and neuraminidase did not increase μ , though neuraminidase cleaved sialic acid from mesothelial glycocalyx of diaphragm specimens, and removed hystochemical stain of sialic acid from glycocalyx. Sialomucin treated with neuraminidase lowered μ of blotted Mesothelium, though less than untreated sialomucin; this feature plus lubrication provided by other molecules could explain why μ did not increase after neuraminidase. Short pronase treatment (in order to affect only glycocalyx proteins) increased μ ; this increase was removed by hyaluronan or sialomucin. After pronase treatment μ decreased with increase in sliding velocity, indicating a regime of mixed lubrication, as in blotted Mesothelium.
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β2-Adrenergic receptors and G-protein-coupled receptor kinase 2 in rabbit pleural Mesothelium.
Respiratory physiology & neurobiology, 2010Co-Authors: Chiara Sironi, Francesca Bodega, Luciano Zocchi, Cristina Porta, Marta Armilli, Emilio AgostoniAbstract:Former studies on net rate of liquid absorption from small Ringer or 1% albumin-Ringer hydrothoraces in rabbits indicated that Na+ transport and solute-coupled liquid absorption by Mesothelium is increased by pleural liquid dilution, and stimulation of β2-adrenoreceptors (β2AR). In this research we tried to provide molecular evidence for β2AR in visceral and parietal Mesothelium of rabbit pleura. Moreover, because prolonged stimulation of β2AR may lead to desensitization mediated by G-protein-coupled receptor kinase 2 (GRK2), we also checked whether GRK2 is expressed in pleural Mesothelium. To this end we performed immunoblot assays on total protein extracts from scraped visceral and parietal Mesothelium, and from cultured pleural mesothelial cells of rabbits. All three samples showed β2AR and GRK2 specific bands.
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na glucose cotransporter is also expressed in Mesothelium of species with thick visceral pleura
Respiratory Physiology & Neurobiology, 2008Co-Authors: Chiara Sironi, Francesca Odega, Ario Monaco, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Molecular evidence for Na+–glucose cotransporter (SGLT1) in rabbit pleural Mesothelium has been recently provided, confirming earlier functional findings on solute-coupled liquid absorption from rabbit pleural space. In this research we checked whether SGLT1 is also expressed in pleural Mesothelium of species with thick visceral pleura, which receives blood from systemic circulation, but drains it into pulmonary veins. To this end immunoblot assays were performed on total protein extract of scraped visceral and parietal Mesothelium of lambs and adult sheep, and of a human mesothelial cell line. All of them showed SGLT1 specific bands. Moreover, confocal immunofluorescence images of lamb pleural Mesothelium showed that SGLT1 is located in apical membrane. Therefore, a solute-coupled liquid absorption should also occur from pleural space of species with thick visceral pleura. Because of this protein-free liquid entering interstitium between visceral Mesothelium and capillaries, inherent Starling forces should be different than hitherto considered, and visceral pleura capillaries could absorb liquid even in these species.
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Na+-glucose cotransporter is also expressed in Mesothelium of species with thick visceral pleura.
Respiratory physiology & neurobiology, 2008Co-Authors: Chiara Sironi, Francesca Bodega, Ario Monaco, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Molecular evidence for Na+–glucose cotransporter (SGLT1) in rabbit pleural Mesothelium has been recently provided, confirming earlier functional findings on solute-coupled liquid absorption from rabbit pleural space. In this research we checked whether SGLT1 is also expressed in pleural Mesothelium of species with thick visceral pleura, which receives blood from systemic circulation, but drains it into pulmonary veins. To this end immunoblot assays were performed on total protein extract of scraped visceral and parietal Mesothelium of lambs and adult sheep, and of a human mesothelial cell line. All of them showed SGLT1 specific bands. Moreover, confocal immunofluorescence images of lamb pleural Mesothelium showed that SGLT1 is located in apical membrane. Therefore, a solute-coupled liquid absorption should also occur from pleural space of species with thick visceral pleura. Because of this protein-free liquid entering interstitium between visceral Mesothelium and capillaries, inherent Starling forces should be different than hitherto considered, and visceral pleura capillaries could absorb liquid even in these species.
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expression of na glucose cotransporter sglt1 in visceral and parietal Mesothelium of rabbit pleura
Respiratory Physiology & Neurobiology, 2007Co-Authors: Chiara Sironi, Francesca Odega, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Indirect evidence for a solute-coupled liquid absorption from rabbit pleural space indicated that it should be caused by a Na + /H + –Cl − /HCO 3 − double exchanger and a Na + –glucose cotransporter [Agostoni, E., Zocchi, L., 1998. Mechanical coupling and liquid exchanges in the pleural space. In: Antony, V.B. (Ed.), Clinics in Chest Medicine: Diseases of the Pleura, vol. 19. Saunders, Philadelphia, pp. 241–260]. In this research we tried to obtain molecular evidence for Na + –glucose cotransporter (SGLT1) in visceral and parietal Mesothelium of rabbit pleura. To this end we performed immunoblot assays on total protein extracts of scraped visceral or parietal Mesothelium of rabbits. These showed two bands: one at 72 kDa (m.w. of SGLT1), and one at 55 kDa (which should also provide Na + –glucose cotransport). Both bands disappeared in assays in which SGLT1 antibody was preadsorbed with specific antigen. Molecular evidence for Na + /K + ATPase (α1 subunit) was also provided. Immunoblot assays for SGLT1 on cultured mesothelial cells of rabbit pleura showed a band at 72 kDa, and in some cases also at 55 kDa, irrespectively of treatment with a differentiating agent. Solute-coupled liquid absorption hinders liquid filtration through parietal Mesothelium caused by Starling forces, and favours liquid absorption through visceral Mesothelium caused by these forces.
Cristina Porta - One of the best experts on this subject based on the ideXlab platform.
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pleural Mesothelium lubrication after hyaluronidase neuraminidase or pronase treatment
Respiratory Physiology & Neurobiology, 2013Co-Authors: Chiara Sironi, Francesca Bodega, Cristina Porta, Emilio AgostoniAbstract:Abstract Coefficient of kinetic friction ( μ ) of pleural Mesothelium has been found to increase markedly after mesothelial blotting and rewetting. This increase disappeared after addition of a solution with hyaluronan or sialomucin, though previous morphological studies showed that only sialomucin occurs in mesothelial glycocalyx. In this research we investigated whether μ of rabbit pleural Mesothelium increased after hyaluronidase, neuraminidase or pronase treatment. Hyaluronidase and neuraminidase did not increase μ , though neuraminidase cleaved sialic acid from mesothelial glycocalyx of diaphragm specimens, and removed hystochemical stain of sialic acid from glycocalyx. Sialomucin treated with neuraminidase lowered μ of blotted Mesothelium, though less than untreated sialomucin; this feature plus lubrication provided by other molecules could explain why μ did not increase after neuraminidase. Short pronase treatment (in order to affect only glycocalyx proteins) increased μ ; this increase was removed by hyaluronan or sialomucin. After pronase treatment μ decreased with increase in sliding velocity, indicating a regime of mixed lubrication, as in blotted Mesothelium.
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β2-Adrenergic receptors and G-protein-coupled receptor kinase 2 in rabbit pleural Mesothelium.
Respiratory physiology & neurobiology, 2010Co-Authors: Chiara Sironi, Francesca Bodega, Luciano Zocchi, Cristina Porta, Marta Armilli, Emilio AgostoniAbstract:Former studies on net rate of liquid absorption from small Ringer or 1% albumin-Ringer hydrothoraces in rabbits indicated that Na+ transport and solute-coupled liquid absorption by Mesothelium is increased by pleural liquid dilution, and stimulation of β2-adrenoreceptors (β2AR). In this research we tried to provide molecular evidence for β2AR in visceral and parietal Mesothelium of rabbit pleura. Moreover, because prolonged stimulation of β2AR may lead to desensitization mediated by G-protein-coupled receptor kinase 2 (GRK2), we also checked whether GRK2 is expressed in pleural Mesothelium. To this end we performed immunoblot assays on total protein extracts from scraped visceral and parietal Mesothelium, and from cultured pleural mesothelial cells of rabbits. All three samples showed β2AR and GRK2 specific bands.
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na glucose cotransporter is also expressed in Mesothelium of species with thick visceral pleura
Respiratory Physiology & Neurobiology, 2008Co-Authors: Chiara Sironi, Francesca Odega, Ario Monaco, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Molecular evidence for Na+–glucose cotransporter (SGLT1) in rabbit pleural Mesothelium has been recently provided, confirming earlier functional findings on solute-coupled liquid absorption from rabbit pleural space. In this research we checked whether SGLT1 is also expressed in pleural Mesothelium of species with thick visceral pleura, which receives blood from systemic circulation, but drains it into pulmonary veins. To this end immunoblot assays were performed on total protein extract of scraped visceral and parietal Mesothelium of lambs and adult sheep, and of a human mesothelial cell line. All of them showed SGLT1 specific bands. Moreover, confocal immunofluorescence images of lamb pleural Mesothelium showed that SGLT1 is located in apical membrane. Therefore, a solute-coupled liquid absorption should also occur from pleural space of species with thick visceral pleura. Because of this protein-free liquid entering interstitium between visceral Mesothelium and capillaries, inherent Starling forces should be different than hitherto considered, and visceral pleura capillaries could absorb liquid even in these species.
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Na+-glucose cotransporter is also expressed in Mesothelium of species with thick visceral pleura.
Respiratory physiology & neurobiology, 2008Co-Authors: Chiara Sironi, Francesca Bodega, Ario Monaco, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Molecular evidence for Na+–glucose cotransporter (SGLT1) in rabbit pleural Mesothelium has been recently provided, confirming earlier functional findings on solute-coupled liquid absorption from rabbit pleural space. In this research we checked whether SGLT1 is also expressed in pleural Mesothelium of species with thick visceral pleura, which receives blood from systemic circulation, but drains it into pulmonary veins. To this end immunoblot assays were performed on total protein extract of scraped visceral and parietal Mesothelium of lambs and adult sheep, and of a human mesothelial cell line. All of them showed SGLT1 specific bands. Moreover, confocal immunofluorescence images of lamb pleural Mesothelium showed that SGLT1 is located in apical membrane. Therefore, a solute-coupled liquid absorption should also occur from pleural space of species with thick visceral pleura. Because of this protein-free liquid entering interstitium between visceral Mesothelium and capillaries, inherent Starling forces should be different than hitherto considered, and visceral pleura capillaries could absorb liquid even in these species.
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expression of na glucose cotransporter sglt1 in visceral and parietal Mesothelium of rabbit pleura
Respiratory Physiology & Neurobiology, 2007Co-Authors: Chiara Sironi, Francesca Odega, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Indirect evidence for a solute-coupled liquid absorption from rabbit pleural space indicated that it should be caused by a Na + /H + –Cl − /HCO 3 − double exchanger and a Na + –glucose cotransporter [Agostoni, E., Zocchi, L., 1998. Mechanical coupling and liquid exchanges in the pleural space. In: Antony, V.B. (Ed.), Clinics in Chest Medicine: Diseases of the Pleura, vol. 19. Saunders, Philadelphia, pp. 241–260]. In this research we tried to obtain molecular evidence for Na + –glucose cotransporter (SGLT1) in visceral and parietal Mesothelium of rabbit pleura. To this end we performed immunoblot assays on total protein extracts of scraped visceral or parietal Mesothelium of rabbits. These showed two bands: one at 72 kDa (m.w. of SGLT1), and one at 55 kDa (which should also provide Na + –glucose cotransport). Both bands disappeared in assays in which SGLT1 antibody was preadsorbed with specific antigen. Molecular evidence for Na + /K + ATPase (α1 subunit) was also provided. Immunoblot assays for SGLT1 on cultured mesothelial cells of rabbit pleura showed a band at 72 kDa, and in some cases also at 55 kDa, irrespectively of treatment with a differentiating agent. Solute-coupled liquid absorption hinders liquid filtration through parietal Mesothelium caused by Starling forces, and favours liquid absorption through visceral Mesothelium caused by these forces.
David M. Bader - One of the best experts on this subject based on the ideXlab platform.
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Investigating the Effect of Mechanical Stimuli on Omental Mesothelium Differentiation
Volume 1A: Abdominal Aortic Aneurysms; Active and Reactive Soft Matter; Atherosclerosis; BioFluid Mechanics; Education; Biotransport Phenomena; Bone J, 2013Co-Authors: Lucas H. Hofmeister, Todd P. Lagus, Elaine L. Shelton, Jon F. Edd, David M. Bader, Hak-joon SungAbstract:Cell sourcing for tissue engineered approaches to vascular repair is a serious issue confronting the field of cardiovascular tissue engineering. Omental Mesothelium is a promising autologous cell source for vascular repair and has been used for numerous other therapies [1]. Until recently, omental Mesothelium was only thought to play a paracrine role in wound healing but there is increasing evidence that omental Mesothelium can undergo divergent terminal differentiation to reparative vasculogenic cell types including: endothelial cells, fibroblasts, or vascular smooth muscle cells.© 2013 ASME
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Development of the Serosal Mesothelium
Journal of Developmental Biology, 2013Co-Authors: Nichelle I. Winters, David M. BaderAbstract:Mesothelia in the adult vertebrate are the simple squamous epithelia covering all coelomic organs and body cavities. Until recently, analysis of the generation and differentiative potential of mesothelia in organogenesis has largely focused on development of visceral Mesothelium of the heart; the epicardium and its progenitor, the proepicardium. Here, we review emerging data on the development and differentiation of serosal Mesothelium, the covering of the gastrointestinal tract. This literature demonstrates that serosal Mesothelium is generated through a completely different mechanism than that seen in the heart suggesting that commitment of progenitors to this cell lineage does not follow a common pathway. The differentiative potential of serosal Mesothelium is also discussed in comparison to that observed for progeny of the proepicardium/epicardium. In our review of the literature, we point out gaps in our understanding of serosal mesothelial development and that of mesothelial development as a whole.
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Mesothelium contributes to vascular smooth muscle and mesenchyme during lung development
Proceedings of the National Academy of Sciences of the United States of America, 2008Co-Authors: Jianwen Que, David M. Bader, Bettina Wilm, Hiroshi Hasegawa, Fan Wang, Brigid L.m. HoganAbstract:During mouse development, the sophisticated vascular network of the lung is established from embryonic day (E) approximately 10.5 and continues to develop postnatally. This network is composed of endothelial cells enclosed by vascular smooth muscle, pericytes, and other mesenchymal cells. Recent in vivo lineage labeling studies in the developing heart and intestine suggest that some of the vascular smooth muscle cells arise from the surface Mesothelium. In the developing lung, the Wilm's tumor 1 gene (Wt1) is expressed only in the mesothelial cells. Therefore, we lineage-labeled the Mesothelium in vivo by using a Wt1-Cre transgene in combination with either Rosa26R(lacZ), Rosa26R(CAG-hPLAP), or Rosa26R(EYFP) reporter alleles. In all three cases, cells derived from lineage-labeled Mesothelium are found inside the lung and as smooth muscle actin (SMA) and PDGF receptor-beta positive cells in the walls of pulmonary blood vessels. To corroborate this finding, we used 5-(and-6)-carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester "mixed isomers" (CCFSE) dye to label mesothelial cells on the surface of the embryonic lung. Over the course of 72-h culture, dye-labeled cells also appear within the lung mesenchyme. Together, our data provide evidence that mesothelial cells serve as a source of vascular smooth muscle cells in the developing lung and suggest that a conserved mechanism applies to the development of blood vessels in all coelomic organs.
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The serosal Mesothelium is a major source of smooth muscle cells of the gut vasculature.
Development (Cambridge England), 2005Co-Authors: Bettina Wilm, Annemieke Ipenberg, Nicholas D. Hastie, John B. E. Burch, David M. BaderAbstract:Most internal organs are situated in a coelomic cavity and are covered by a Mesothelium. During heart development, epicardial cells (a Mesothelium) move to and over the heart, undergo epithelial-mesenchymal transition (EMT), and subsequently differentiate into endothelial and vascular smooth muscle cells. This is thought to be a unique process in blood vessel formation. Still, structural and developmental similarities between the heart and gut led us to test the hypothesis that a conserved or related mechanism may regulate blood vessel development to the gut, which, similar to the heart, is housed in a coelomic cavity. By using a combination of molecular genetics, vital dye fate mapping, organ culture and immunohistochemistry, we demonstrate that the serosal Mesothelium is the major source of vasculogenic cells in developing mouse gut. Our studies show that the gut is initially devoid of a Mesothelium but that serosal mesothelial cells expressing the Wilm's tumor protein (Wt1) move to and over the gut. Subsequently, a subset of these cells undergoes EMT and migrates throughout the gut. Using Wt1-Cre genetic lineage marking of serosal cells and their progeny, we demonstrate that these cells differentiate to smooth muscle of all major blood vessels in the mesenteries and gut. Our data reveal a conserved mechanism in blood vessel formation to coelomic organs, and have major implications for our understanding of vertebrate organogenesis and vascular deficiencies of the gut.
Luciano Zocchi - One of the best experts on this subject based on the ideXlab platform.
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β2-Adrenergic receptors and G-protein-coupled receptor kinase 2 in rabbit pleural Mesothelium.
Respiratory physiology & neurobiology, 2010Co-Authors: Chiara Sironi, Francesca Bodega, Luciano Zocchi, Cristina Porta, Marta Armilli, Emilio AgostoniAbstract:Former studies on net rate of liquid absorption from small Ringer or 1% albumin-Ringer hydrothoraces in rabbits indicated that Na+ transport and solute-coupled liquid absorption by Mesothelium is increased by pleural liquid dilution, and stimulation of β2-adrenoreceptors (β2AR). In this research we tried to provide molecular evidence for β2AR in visceral and parietal Mesothelium of rabbit pleura. Moreover, because prolonged stimulation of β2AR may lead to desensitization mediated by G-protein-coupled receptor kinase 2 (GRK2), we also checked whether GRK2 is expressed in pleural Mesothelium. To this end we performed immunoblot assays on total protein extracts from scraped visceral and parietal Mesothelium, and from cultured pleural mesothelial cells of rabbits. All three samples showed β2AR and GRK2 specific bands.
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na glucose cotransporter is also expressed in Mesothelium of species with thick visceral pleura
Respiratory Physiology & Neurobiology, 2008Co-Authors: Chiara Sironi, Francesca Odega, Ario Monaco, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Molecular evidence for Na+–glucose cotransporter (SGLT1) in rabbit pleural Mesothelium has been recently provided, confirming earlier functional findings on solute-coupled liquid absorption from rabbit pleural space. In this research we checked whether SGLT1 is also expressed in pleural Mesothelium of species with thick visceral pleura, which receives blood from systemic circulation, but drains it into pulmonary veins. To this end immunoblot assays were performed on total protein extract of scraped visceral and parietal Mesothelium of lambs and adult sheep, and of a human mesothelial cell line. All of them showed SGLT1 specific bands. Moreover, confocal immunofluorescence images of lamb pleural Mesothelium showed that SGLT1 is located in apical membrane. Therefore, a solute-coupled liquid absorption should also occur from pleural space of species with thick visceral pleura. Because of this protein-free liquid entering interstitium between visceral Mesothelium and capillaries, inherent Starling forces should be different than hitherto considered, and visceral pleura capillaries could absorb liquid even in these species.
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Na+-glucose cotransporter is also expressed in Mesothelium of species with thick visceral pleura.
Respiratory physiology & neurobiology, 2008Co-Authors: Chiara Sironi, Francesca Bodega, Ario Monaco, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Molecular evidence for Na+–glucose cotransporter (SGLT1) in rabbit pleural Mesothelium has been recently provided, confirming earlier functional findings on solute-coupled liquid absorption from rabbit pleural space. In this research we checked whether SGLT1 is also expressed in pleural Mesothelium of species with thick visceral pleura, which receives blood from systemic circulation, but drains it into pulmonary veins. To this end immunoblot assays were performed on total protein extract of scraped visceral and parietal Mesothelium of lambs and adult sheep, and of a human mesothelial cell line. All of them showed SGLT1 specific bands. Moreover, confocal immunofluorescence images of lamb pleural Mesothelium showed that SGLT1 is located in apical membrane. Therefore, a solute-coupled liquid absorption should also occur from pleural space of species with thick visceral pleura. Because of this protein-free liquid entering interstitium between visceral Mesothelium and capillaries, inherent Starling forces should be different than hitherto considered, and visceral pleura capillaries could absorb liquid even in these species.
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expression of na glucose cotransporter sglt1 in visceral and parietal Mesothelium of rabbit pleura
Respiratory Physiology & Neurobiology, 2007Co-Authors: Chiara Sironi, Francesca Odega, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Indirect evidence for a solute-coupled liquid absorption from rabbit pleural space indicated that it should be caused by a Na + /H + –Cl − /HCO 3 − double exchanger and a Na + –glucose cotransporter [Agostoni, E., Zocchi, L., 1998. Mechanical coupling and liquid exchanges in the pleural space. In: Antony, V.B. (Ed.), Clinics in Chest Medicine: Diseases of the Pleura, vol. 19. Saunders, Philadelphia, pp. 241–260]. In this research we tried to obtain molecular evidence for Na + –glucose cotransporter (SGLT1) in visceral and parietal Mesothelium of rabbit pleura. To this end we performed immunoblot assays on total protein extracts of scraped visceral or parietal Mesothelium of rabbits. These showed two bands: one at 72 kDa (m.w. of SGLT1), and one at 55 kDa (which should also provide Na + –glucose cotransport). Both bands disappeared in assays in which SGLT1 antibody was preadsorbed with specific antigen. Molecular evidence for Na + /K + ATPase (α1 subunit) was also provided. Immunoblot assays for SGLT1 on cultured mesothelial cells of rabbit pleura showed a band at 72 kDa, and in some cases also at 55 kDa, irrespectively of treatment with a differentiating agent. Solute-coupled liquid absorption hinders liquid filtration through parietal Mesothelium caused by Starling forces, and favours liquid absorption through visceral Mesothelium caused by these forces.
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Expression of Na+-glucose cotransporter (SGLT1) in visceral and parietal Mesothelium of rabbit pleura.
Respiratory physiology & neurobiology, 2007Co-Authors: Chiara Sironi, Francesca Bodega, Luciano Zocchi, Cristina Porta, Emilio AgostoniAbstract:Abstract Indirect evidence for a solute-coupled liquid absorption from rabbit pleural space indicated that it should be caused by a Na + /H + –Cl − /HCO 3 − double exchanger and a Na + –glucose cotransporter [Agostoni, E., Zocchi, L., 1998. Mechanical coupling and liquid exchanges in the pleural space. In: Antony, V.B. (Ed.), Clinics in Chest Medicine: Diseases of the Pleura, vol. 19. Saunders, Philadelphia, pp. 241–260]. In this research we tried to obtain molecular evidence for Na + –glucose cotransporter (SGLT1) in visceral and parietal Mesothelium of rabbit pleura. To this end we performed immunoblot assays on total protein extracts of scraped visceral or parietal Mesothelium of rabbits. These showed two bands: one at 72 kDa (m.w. of SGLT1), and one at 55 kDa (which should also provide Na + –glucose cotransport). Both bands disappeared in assays in which SGLT1 antibody was preadsorbed with specific antigen. Molecular evidence for Na + /K + ATPase (α1 subunit) was also provided. Immunoblot assays for SGLT1 on cultured mesothelial cells of rabbit pleura showed a band at 72 kDa, and in some cases also at 55 kDa, irrespectively of treatment with a differentiating agent. Solute-coupled liquid absorption hinders liquid filtration through parietal Mesothelium caused by Starling forces, and favours liquid absorption through visceral Mesothelium caused by these forces.