The Experts below are selected from a list of 39 Experts worldwide ranked by ideXlab platform
Jong-yil Chai - One of the best experts on this subject based on the ideXlab platform.
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Decrease of Metagonimus yokogawai endemicity along the Tamjin River basin.
The Korean Journal of Parasitology, 2008Co-Authors: Jin-ju Lee, Hyo-jin Kim, Min Jae Kim, Jo Woon Yi Lee, Bong-kwang Jung, Jiyoun Lee, Eun-hee Shin, Jae-lip Kim, Jong-yil ChaiAbstract:The Tamjin River which flows from Jangheung-gun via Gangjin-gun to the South Sea was reported to be a highly endemic area of Metagonimus yokogawai infection in 1977 and 1985. However, there were no recent studies demonstrating how much change occurred in the endemicity, in terms of prevalence and worm burden, of Metagonimiasis in this river basin. Thus, a small-scale epidemiological survey was carried out on some residents along the Tamjin River basin in order to determine the current status of M. yokogawai infection. A total of 48 fecal samples were collected and examined by the Kato-Katz thick smear and formalin-ether sedimentation techniques. The egg positive rate of all helminths was 50.0%, and that of M. yokogawai was 37.5%, followed by C. sinensis 22.9% and G. seoi 4.2%. To obtain the adult flukes of M. yokogawai, 6 egg positive cases were treated with praziquantel 10 mg/kg in a single dose and purged with magnesium sulfate. A total of 5,225 adult flukes (average 871 specimens per person) of M. yokogawai were collected from their diarrheic stools. Compared with the data reported in 1977 and 1985, the individual worm burdens appeared to have decreased remarkably, although the prevalence did not decrease at all. It is suggested that the endemicity of M. yokogawai infection along the Tamjin River has been reduced. To confirm this suggestion, the status of infection in snail and fish intermediate hosts should be investigated.
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of metagonimus prevalent in the republic of korea
2005Co-Authors: Sang-mee Guk, Jae-lip Kim, Jin Young Park, Min Seot, Euntaek Hant, Jong-yil ChaiAbstract:Susceptibility of inbred mouse strains to Metagonimus yokogawai, Metagonimus miyatai, and Metagonimus taka- hashii infections was studied using BALB/c, ddY, C57BL/6J, C3H/HeN, and A/J mice, with H-2 haplotypes d, s, b, k, and a, respectively. Two hundred metacercariae were orally fed to each mouse, and the worm recovery rates (WRR), worm dimensions, and intrauterine egg numbers were measured at days 3, 7, 14, 21, and 28 postinfection (PI). On day 14 PI, the WRR of M. yokogawai was highest in ddY mice (average, 62.2%); those of M. miyatai and M. takahashii were highest in ddY (19.5%) and BALB/c mice (10.4%), respectively; worm maturation was best in C3H/HeN (M. yokogawai), C57BL/6J (M. miyatai), and ddY mice (M. takahashii). All mouse strains showed higher susceptibility to infection with M. yokogawai than with M. miyatai or M. takahashii. The results show that susceptibility of mice to Metagonimus infection varies according to mouse strain and parasite species but is suggested to be independent of the mouse H-2 haplotype. In the Republic of Korea, Metagonimus yokogawai (Digenea: Heterophyidae) is known as the major species responsible for human Metagonimiasis (Chai and Lee, 2002). This fluke is dis- tributed widely along rivers and streams where the sweetfish, Plecoglossus altivelis, are available (Chai, Han et al., 2000; Chai and Lee, 2002). Two other species causing human meta- gonimiasis in the Republic of Korea are Metagonimus miyatai and Metagonimus takahashii, which produce large-sized eggs and are transmitted by different fish hosts, i.e., the minnow, Zacco platypus, and the crucian carp, Carassius auratus, re-
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Effects of gamma-irradiation on the survival and development of Metagonimus yokogawai metacercariae in rats.
The Korean Journal of Parasitology, 1995Co-Authors: Jong-yil Chai, Sang-joon Kim, Jina KookAbstract:To evaluate the feasibility of irradiation as a control measure for Metagonimiasis, the metacercariae of Metagonimus yokogawai were irradiated with gamma ray, either after isolation from the sweetfish (Plecoglossus altivelis) or in situ of the fish, and their survival and development in rats were observed at 7 days post-infection. The radiation dose varied from 5 to 100 Gy for the metacercaria-irradiation group and from 5 to 500 Gy for fish-irradiation group. The results showed that the worm recovery rate from the irradiation groups decreased as the radiation dose was increased. Higher doses of radiation were required for the fish-irradiation group to obtain the same results as the metacercaria-irradiation group. The LD50 of the metacercaria-irradiation group was 4.5 Gy, whereas that of the fish-irradiation group 6.2 Gy. A few number of worms which survived until 7 days in rats were severely retarded especially in the growth of their reproductive organs, i.e., complete or partial failure in the development of testes and formation of uterine eggs. The present study revealed that irradiation of sweetfish by 200 Gy is effective to control infectivity as well as development of M. yokogawai metacercaria in rats.
Urusa Thaenkham - One of the best experts on this subject based on the ideXlab platform.
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a molecular phylogeny of asian species of the genus metagonimus digenea small intestinal flukes based on representative japanese populations
Parasitology Research, 2016Co-Authors: Siritavee Pornruseetairatn, Takeshi Shimazu, Hideto Kino, Yukifumi Nawa, Tomáš Scholz, Jiraporn Ruangsittichai, Naowarat Tanomsing Saralamba, Urusa ThaenkhamAbstract:Metagonimus Katsurada, 1912 is a genus of small intestinal parasites. The genus comprises eight species, primarily from far-eastern Asia, with two exceptions reported from Europe. Metagonimus yokogawai, the most widespread species, is the main agent responsible for the intestinal disease, Metagonimiasis, in Japan and some other East Asian countries. On the basis of the ratio of the size of the ventral and oral suckers, Metagonimus has traditionally been morphologically divided into two groups; however, the genus has not been extensively studied using molecular data. To reveal phylogenetic relationships within Metagonimus based on molecular data, we analyzed six of the seven species present in Asia using samples collected in central Japan. Maximum likelihood and Bayesian analyses of a combined 28S ribosomal DNA (rDNA), internal transcribed spacer 2 (ITS2), and mitochondrial cox1 gene sequence dataset separated the six species into two well-supported clades. One clade comprised M. yokogawai, M. takahashii, M. miyatai, and M. hakubaensis, whereas the other consisted of M. otsurui and M. katsuradai. Genetic distances calculated from 28S rDNA and ITS2 nucleotide sequences and a comparison of the predicted amino acid sequences of cox1 gene suggested that M. otsurui and M. katsuradai may have diverged recently. None of the four main morphological characters used to delimit species of Metagonimus (i.e., sucker ratio, positions of the uterus and testes, and distribution of vitelline follicles) was consistent with the distribution of species in the molecular tree.
Siritavee Pornruseetairatn - One of the best experts on this subject based on the ideXlab platform.
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a molecular phylogeny of asian species of the genus metagonimus digenea small intestinal flukes based on representative japanese populations
Parasitology Research, 2016Co-Authors: Siritavee Pornruseetairatn, Takeshi Shimazu, Hideto Kino, Yukifumi Nawa, Tomáš Scholz, Jiraporn Ruangsittichai, Naowarat Tanomsing Saralamba, Urusa ThaenkhamAbstract:Metagonimus Katsurada, 1912 is a genus of small intestinal parasites. The genus comprises eight species, primarily from far-eastern Asia, with two exceptions reported from Europe. Metagonimus yokogawai, the most widespread species, is the main agent responsible for the intestinal disease, Metagonimiasis, in Japan and some other East Asian countries. On the basis of the ratio of the size of the ventral and oral suckers, Metagonimus has traditionally been morphologically divided into two groups; however, the genus has not been extensively studied using molecular data. To reveal phylogenetic relationships within Metagonimus based on molecular data, we analyzed six of the seven species present in Asia using samples collected in central Japan. Maximum likelihood and Bayesian analyses of a combined 28S ribosomal DNA (rDNA), internal transcribed spacer 2 (ITS2), and mitochondrial cox1 gene sequence dataset separated the six species into two well-supported clades. One clade comprised M. yokogawai, M. takahashii, M. miyatai, and M. hakubaensis, whereas the other consisted of M. otsurui and M. katsuradai. Genetic distances calculated from 28S rDNA and ITS2 nucleotide sequences and a comparison of the predicted amino acid sequences of cox1 gene suggested that M. otsurui and M. katsuradai may have diverged recently. None of the four main morphological characters used to delimit species of Metagonimus (i.e., sucker ratio, positions of the uterus and testes, and distribution of vitelline follicles) was consistent with the distribution of species in the molecular tree.
Takeshi Shimazu - One of the best experts on this subject based on the ideXlab platform.
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a molecular phylogeny of asian species of the genus metagonimus digenea small intestinal flukes based on representative japanese populations
Parasitology Research, 2016Co-Authors: Siritavee Pornruseetairatn, Takeshi Shimazu, Hideto Kino, Yukifumi Nawa, Tomáš Scholz, Jiraporn Ruangsittichai, Naowarat Tanomsing Saralamba, Urusa ThaenkhamAbstract:Metagonimus Katsurada, 1912 is a genus of small intestinal parasites. The genus comprises eight species, primarily from far-eastern Asia, with two exceptions reported from Europe. Metagonimus yokogawai, the most widespread species, is the main agent responsible for the intestinal disease, Metagonimiasis, in Japan and some other East Asian countries. On the basis of the ratio of the size of the ventral and oral suckers, Metagonimus has traditionally been morphologically divided into two groups; however, the genus has not been extensively studied using molecular data. To reveal phylogenetic relationships within Metagonimus based on molecular data, we analyzed six of the seven species present in Asia using samples collected in central Japan. Maximum likelihood and Bayesian analyses of a combined 28S ribosomal DNA (rDNA), internal transcribed spacer 2 (ITS2), and mitochondrial cox1 gene sequence dataset separated the six species into two well-supported clades. One clade comprised M. yokogawai, M. takahashii, M. miyatai, and M. hakubaensis, whereas the other consisted of M. otsurui and M. katsuradai. Genetic distances calculated from 28S rDNA and ITS2 nucleotide sequences and a comparison of the predicted amino acid sequences of cox1 gene suggested that M. otsurui and M. katsuradai may have diverged recently. None of the four main morphological characters used to delimit species of Metagonimus (i.e., sucker ratio, positions of the uterus and testes, and distribution of vitelline follicles) was consistent with the distribution of species in the molecular tree.
Tomáš Scholz - One of the best experts on this subject based on the ideXlab platform.
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a molecular phylogeny of asian species of the genus metagonimus digenea small intestinal flukes based on representative japanese populations
Parasitology Research, 2016Co-Authors: Siritavee Pornruseetairatn, Takeshi Shimazu, Hideto Kino, Yukifumi Nawa, Tomáš Scholz, Jiraporn Ruangsittichai, Naowarat Tanomsing Saralamba, Urusa ThaenkhamAbstract:Metagonimus Katsurada, 1912 is a genus of small intestinal parasites. The genus comprises eight species, primarily from far-eastern Asia, with two exceptions reported from Europe. Metagonimus yokogawai, the most widespread species, is the main agent responsible for the intestinal disease, Metagonimiasis, in Japan and some other East Asian countries. On the basis of the ratio of the size of the ventral and oral suckers, Metagonimus has traditionally been morphologically divided into two groups; however, the genus has not been extensively studied using molecular data. To reveal phylogenetic relationships within Metagonimus based on molecular data, we analyzed six of the seven species present in Asia using samples collected in central Japan. Maximum likelihood and Bayesian analyses of a combined 28S ribosomal DNA (rDNA), internal transcribed spacer 2 (ITS2), and mitochondrial cox1 gene sequence dataset separated the six species into two well-supported clades. One clade comprised M. yokogawai, M. takahashii, M. miyatai, and M. hakubaensis, whereas the other consisted of M. otsurui and M. katsuradai. Genetic distances calculated from 28S rDNA and ITS2 nucleotide sequences and a comparison of the predicted amino acid sequences of cox1 gene suggested that M. otsurui and M. katsuradai may have diverged recently. None of the four main morphological characters used to delimit species of Metagonimus (i.e., sucker ratio, positions of the uterus and testes, and distribution of vitelline follicles) was consistent with the distribution of species in the molecular tree.