The Experts below are selected from a list of 15 Experts worldwide ranked by ideXlab platform
Jason W. H. Wong - One of the best experts on this subject based on the ideXlab platform.
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Tyrosine nitration moderates the peptidase activity of human Methionyl Aminopeptidase 2.
Biochemical and biophysical research communications, 2013Co-Authors: Joyce Chiu, Philip J. Hogg, Jason W. H. WongAbstract:Methionyl Aminopeptidase 2 (MetAP2) plays an important role in the regulation of angiogenesis. This study examined whether nitration of MetAP2 alters its enzymatic activity in vitro. The activity of unmodified, nitrated and oxidised MetAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in MetAP2. Structural and evolutionary analysis indicate that this is an important residue for MetAP2 activity. Combined, the results show that the activity of MetAP2 is reduced by nitration and raise the possibility that nitration of MetAP2 is a mechanism contributing to endothelial dysfunction.
Joyce Chiu - One of the best experts on this subject based on the ideXlab platform.
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Tyrosine nitration moderates the peptidase activity of human Methionyl Aminopeptidase 2.
Biochemical and biophysical research communications, 2013Co-Authors: Joyce Chiu, Philip J. Hogg, Jason W. H. WongAbstract:Methionyl Aminopeptidase 2 (MetAP2) plays an important role in the regulation of angiogenesis. This study examined whether nitration of MetAP2 alters its enzymatic activity in vitro. The activity of unmodified, nitrated and oxidised MetAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in MetAP2. Structural and evolutionary analysis indicate that this is an important residue for MetAP2 activity. Combined, the results show that the activity of MetAP2 is reduced by nitration and raise the possibility that nitration of MetAP2 is a mechanism contributing to endothelial dysfunction.
Philip J. Hogg - One of the best experts on this subject based on the ideXlab platform.
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Tyrosine nitration moderates the peptidase activity of human Methionyl Aminopeptidase 2.
Biochemical and biophysical research communications, 2013Co-Authors: Joyce Chiu, Philip J. Hogg, Jason W. H. WongAbstract:Methionyl Aminopeptidase 2 (MetAP2) plays an important role in the regulation of angiogenesis. This study examined whether nitration of MetAP2 alters its enzymatic activity in vitro. The activity of unmodified, nitrated and oxidised MetAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in MetAP2. Structural and evolutionary analysis indicate that this is an important residue for MetAP2 activity. Combined, the results show that the activity of MetAP2 is reduced by nitration and raise the possibility that nitration of MetAP2 is a mechanism contributing to endothelial dysfunction.
Rajendra K. Sharma - One of the best experts on this subject based on the ideXlab platform.
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METAP2 (Methionyl Aminopeptidase 2)
Atlas of Genetics and Cytogenetics in Oncology and Haematology, 2011Co-Authors: Ponniah Selvakumar, Rajendra K. SharmaAbstract:Review on METAP2 (Methionyl Aminopeptidase 2), with data on DNA, on the protein encoded, and where the gene is implicated.
Wong, Jason W H - One of the best experts on this subject based on the ideXlab platform.
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Tyrosine nitration moderates the peptidase activity of human Methionyl Aminopeptidase 2
'Elsevier BV', 2013Co-Authors: Ng, John Y., Hogg, Philip J., Chiu Joyce, Wong, Jason W HAbstract:Methionyl Aminopeptidase 2 (MetAP2) plays an important role in the regulation of angiogenesis. This study examined whether nitration of MetAP2 alters its enzymatic activity in vitro. The activity of unmodified, nitrated and oxidised MetAP2 was assessed and it was found that nitration significantly reduced its ability to cleave a chromogenic substrate. Mass spectrometry analysis identified Tyr336 as a nitrated residue in MetAP2. Structural and evolutionary analysis indicate that this is an important residue for MetAP2 activity. Combined, the results show that the activity of MetAP2 is reduced by nitration and raise the possibility that nitration of MetAP2 is a mechanism contributing to endothelial dysfunction. © 2013 Elsevier Inc.Link_to_subscribed_fulltex