The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform

Ashish K Singh - One of the best experts on this subject based on the ideXlab platform.

  • Production of β-galactosidase from streptococcus thermophilus for galactooligosaccharides synthesis
    Journal of Food Science and Technology, 2015
    Co-Authors: Vikas Sangwan, Ram R. B. Singh, Babar Ali, Sudhir Kumar Tomar, Ashish K Singh
    Abstract:

    Efficiency of different Methods for disruption of Streptococcus thermophilus Cells, isolated from different dairy products, to release β-galactosidase and synthesis of GOS by extracted enzyme using whey supplemented with different concentrations of lactose as a substrate was studied. Unlike most other studies on GOS synthesis which used only one Method of Cell disruption and only few microbial strains, we compared five different Cell disruption Methods and used 30 strains of S. thermophilus in order to find out the most effective Method and efficient strain for production of β-galactosidase. Appreciable amount of GOS (53.45 gL−1) was synthesized at a lactose concentration of 30 %, using enzyme (10 U mL−1 of reaction medium), extracted from S. thermophilus within a very short incubation time of 5 h at a temperature of 40 °C and pH 6.8. S. thermophilus is heavily employed in the preparation of fermented dairy products but this study extends the use of this organism for the production of GOS, a potential prebiotic.

  • Production of β-galactosidase from streptococcus thermophilus for galactooligosaccharides synthesis
    Journal of Food Science and Technology, 2015
    Co-Authors: Vikas Sangwan, Ram R. B. Singh, Babar Ali, Sudhir Kumar Tomar, Ashish K Singh
    Abstract:

    Efficiency of different Methods for disruption of Streptococcus thermophilus Cells, isolated from different dairy products, to release β-galactosidase and synthesis of GOS by extracted enzyme using whey supplemented with different concentrations of lactose as a substrate was studied. Unlike most other studies on GOS synthesis which used only one Method of Cell disruption and only few microbial strains, we compared five different Cell disruption Methods and used 30 strains of S. thermophilus in order to find out the most effective Method and efficient strain for production of β-galactosidase. Appreciable amount of GOS (53.45 gL−1) was synthesized at a lactose concentration of 30 %, using enzyme (10 U mL−1 of reaction medium), extracted from S. thermophilus within a very short incubation time of 5 h at a temperature of 40 °C and pH 6.8. S. thermophilus is heavily employed in the preparation of fermented dairy products but this study extends the use of this organism for the production of GOS, a potential prebiotic.

Vikas Sangwan - One of the best experts on this subject based on the ideXlab platform.

  • Production of β-galactosidase from streptococcus thermophilus for galactooligosaccharides synthesis
    Journal of Food Science and Technology, 2015
    Co-Authors: Vikas Sangwan, Ram R. B. Singh, Babar Ali, Sudhir Kumar Tomar, Ashish K Singh
    Abstract:

    Efficiency of different Methods for disruption of Streptococcus thermophilus Cells, isolated from different dairy products, to release β-galactosidase and synthesis of GOS by extracted enzyme using whey supplemented with different concentrations of lactose as a substrate was studied. Unlike most other studies on GOS synthesis which used only one Method of Cell disruption and only few microbial strains, we compared five different Cell disruption Methods and used 30 strains of S. thermophilus in order to find out the most effective Method and efficient strain for production of β-galactosidase. Appreciable amount of GOS (53.45 gL−1) was synthesized at a lactose concentration of 30 %, using enzyme (10 U mL−1 of reaction medium), extracted from S. thermophilus within a very short incubation time of 5 h at a temperature of 40 °C and pH 6.8. S. thermophilus is heavily employed in the preparation of fermented dairy products but this study extends the use of this organism for the production of GOS, a potential prebiotic.

  • Production of β-galactosidase from streptococcus thermophilus for galactooligosaccharides synthesis
    Journal of Food Science and Technology, 2015
    Co-Authors: Vikas Sangwan, Ram R. B. Singh, Babar Ali, Sudhir Kumar Tomar, Ashish K Singh
    Abstract:

    Efficiency of different Methods for disruption of Streptococcus thermophilus Cells, isolated from different dairy products, to release β-galactosidase and synthesis of GOS by extracted enzyme using whey supplemented with different concentrations of lactose as a substrate was studied. Unlike most other studies on GOS synthesis which used only one Method of Cell disruption and only few microbial strains, we compared five different Cell disruption Methods and used 30 strains of S. thermophilus in order to find out the most effective Method and efficient strain for production of β-galactosidase. Appreciable amount of GOS (53.45 gL−1) was synthesized at a lactose concentration of 30 %, using enzyme (10 U mL−1 of reaction medium), extracted from S. thermophilus within a very short incubation time of 5 h at a temperature of 40 °C and pH 6.8. S. thermophilus is heavily employed in the preparation of fermented dairy products but this study extends the use of this organism for the production of GOS, a potential prebiotic.

Arun K Dhar - One of the best experts on this subject based on the ideXlab platform.

  • An improved Method of Cell culture system from eye stalk, hepatopancreas, muscle, ovary, and hemocytes of Penaeus vannamei
    In Vitro Cellular & Developmental Biology - Animal, 2010
    Co-Authors: Sunil K George, Arun K Dhar
    Abstract:

    Improved Methods of Cell culture from eye stalk, hepatopancreas, muscle, ovary, and hemocytes of shrimp ( Penaeus vannamei ) were established using synthetic media and shrimp muscle extract (SME). For hemocytes and ovarian Cell cultures, Grace’s insect medium supplemented with 10% ( v / v ) fetal bovine serum and 10% SME ( v / v ) showed enhanced attachment and proliferation of the Cells. The hemocyte and ovarian Cell cultures could be maintained for 48 and 66 days, respectively, and have been sub-cultured four and six times, respectively. Both ovary and hemocyte Cell cultures contained primarily epithelial-like Cells. Cells derived from ovary tissue grew preferably between 26°C and 28°C with 5% CO_2. Although the temperature preference of hemocyte Cells was the same as ovarian Cells, CO_2 supplementation did not show any difference in the growth of hemocyte Cells. When the shrimp were injected with lipopolysaccharide (8 μg/g of shrimp) and hemolymph was drawn 24 h post-injection, the in vitro multiplicity of hemocytes dramatically improved. The growth of eye stalk, hepatopancreas, and muscle-derived Cells was much less compared to ovarian Cells and hemocytes under the conditions described above. The optimal culture conditions for ovarian Cells and hemocytes were also different from that for eye stalk, hepatopancreas, and muscle Cell culture. The proliferation efficiencies of primary cultures of hepatopancreas, eyestalk, and muscle Cells were about 30, 12, and

  • an improved Method of Cell culture system from eye stalk hepatopancreas muscle ovary and hemocytes of penaeus vannamei
    In Vitro Cellular & Developmental Biology – Animal, 2010
    Co-Authors: Sunil K George, Arun K Dhar
    Abstract:

    Improved Methods of Cell culture from eye stalk, hepatopancreas, muscle, ovary, and hemocytes of shrimp (Penaeus vannamei) were established using synthetic media and shrimp muscle extract (SME). For hemocytes and ovarian Cell cultures, Grace’s insect medium supplemented with 10% (v/v) fetal bovine serum and 10% SME (v/v) showed enhanced attachment and proliferation of the Cells. The hemocyte and ovarian Cell cultures could be maintained for 48 and 66 days, respectively, and have been sub-cultured four and six times, respectively. Both ovary and hemocyte Cell cultures contained primarily epithelial-like Cells. Cells derived from ovary tissue grew preferably between 26°C and 28°C with 5% CO2. Although the temperature preference of hemocyte Cells was the same as ovarian Cells, CO2 supplementation did not show any difference in the growth of hemocyte Cells. When the shrimp were injected with lipopolysaccharide (8 μg/g of shrimp) and hemolymph was drawn 24 h post-injection, the in vitro multiplicity of hemocytes dramatically improved. The growth of eye stalk, hepatopancreas, and muscle-derived Cells was much less compared to ovarian Cells and hemocytes under the conditions described above. The optimal culture conditions for ovarian Cells and hemocytes were also different from that for eye stalk, hepatopancreas, and muscle Cell culture. The proliferation efficiencies of primary cultures of hepatopancreas, eyestalk, and muscle Cells were about 30, 12, and <7 d, respectively. The improved culture conditions described here, particularly for hemocytes and ovary, will be very useful for in vitro studies involving viruses infecting shrimp and in shrimp genomic studies.

Hiroshi Murakami - One of the best experts on this subject based on the ideXlab platform.

  • production of alpha 1 3 galactosyltransferase gene deficient pigs by somatic Cell nuclear transfer a novel selection Method for gal alpha 1 3 gal antigen deficient Cells
    Molecular Reproduction and Development, 2008
    Co-Authors: Tatsuya Fujimura, Yoichi Takahagi, Tamotsu Shigehisa, Hiroshi Nagashima, Shuji Miyagawa, Ryota Shirakura, Hiroshi Murakami
    Abstract:

    The objective of the present study was to isolate alpha 1,3-galactosyltransferase (GalGT)-gene double knockout (DKO) Cells using a novel simple Method of Cell selection Method. To obtain GalGT-DKO Cells, GalGT-gene single knockout (SKO) fetal fibroblast Cells were cultured for three to nine passages and GalGT-null Cells were separated using a biotin-labeled IB4 lectin attached to streptavidin-coated magnetic beads. After 15-17 days of additional cultivation, seven GalGT-DKO Cell colonies were obtained from a total of 2.5 x 10(7) GalGT-SKO Cells. A total of 926 somatic nuclear transferred embryos reconstructed with the DKO Cells were transferred into eight recipient pigs, producing four farrowed, three liveborns, and six stillborns. Absence of GalGT gene in the cloned pigs was confirmed by PCR and Southern blotting. Flow cytometric analysis revealed that alphaGal antigens were not present in the Cells of the cloned DKO pigs.

Ram R. B. Singh - One of the best experts on this subject based on the ideXlab platform.

  • Production of β-galactosidase from streptococcus thermophilus for galactooligosaccharides synthesis
    Journal of Food Science and Technology, 2015
    Co-Authors: Vikas Sangwan, Ram R. B. Singh, Babar Ali, Sudhir Kumar Tomar, Ashish K Singh
    Abstract:

    Efficiency of different Methods for disruption of Streptococcus thermophilus Cells, isolated from different dairy products, to release β-galactosidase and synthesis of GOS by extracted enzyme using whey supplemented with different concentrations of lactose as a substrate was studied. Unlike most other studies on GOS synthesis which used only one Method of Cell disruption and only few microbial strains, we compared five different Cell disruption Methods and used 30 strains of S. thermophilus in order to find out the most effective Method and efficient strain for production of β-galactosidase. Appreciable amount of GOS (53.45 gL−1) was synthesized at a lactose concentration of 30 %, using enzyme (10 U mL−1 of reaction medium), extracted from S. thermophilus within a very short incubation time of 5 h at a temperature of 40 °C and pH 6.8. S. thermophilus is heavily employed in the preparation of fermented dairy products but this study extends the use of this organism for the production of GOS, a potential prebiotic.

  • Production of β-galactosidase from streptococcus thermophilus for galactooligosaccharides synthesis
    Journal of Food Science and Technology, 2015
    Co-Authors: Vikas Sangwan, Ram R. B. Singh, Babar Ali, Sudhir Kumar Tomar, Ashish K Singh
    Abstract:

    Efficiency of different Methods for disruption of Streptococcus thermophilus Cells, isolated from different dairy products, to release β-galactosidase and synthesis of GOS by extracted enzyme using whey supplemented with different concentrations of lactose as a substrate was studied. Unlike most other studies on GOS synthesis which used only one Method of Cell disruption and only few microbial strains, we compared five different Cell disruption Methods and used 30 strains of S. thermophilus in order to find out the most effective Method and efficient strain for production of β-galactosidase. Appreciable amount of GOS (53.45 gL−1) was synthesized at a lactose concentration of 30 %, using enzyme (10 U mL−1 of reaction medium), extracted from S. thermophilus within a very short incubation time of 5 h at a temperature of 40 °C and pH 6.8. S. thermophilus is heavily employed in the preparation of fermented dairy products but this study extends the use of this organism for the production of GOS, a potential prebiotic.