The Experts below are selected from a list of 237 Experts worldwide ranked by ideXlab platform
Justine Clark - One of the best experts on this subject based on the ideXlab platform.
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the genome of deep sea vent chemolithoautotroph thiomicrospira crunogena xcl 2
PLOS Biology, 2006Co-Authors: Kathleen M Scott, Fereniki N Abril, Lois A Ball, Chantell J Barrett, Rodrigo A Blake, Amanda J Boller, Patrick S G Chain, Stefan M Sievert, Justine ClarkAbstract:Presented here is the complete genome sequence of Thiomicrospira crunogena XCL-2, representative of ubiquitous chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 base pairs), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. It has 14 Methyl-Accepting Chemotaxis Protein genes, including four that may assist in positioning it in the redoxcline. A relative abundance of coding sequences (CDSs) encoding regulatory Proteins likely control the expression of genes encoding carboxysomes, multiple dissolved inorganic nitrogen and phosphate transporters, as well as a phosphonate operon, which provide this species with a variety of options for acquiring these substrates from the environment. Thiom. crunogena XCL-2 is unusual among obligate sulfur-oxidizing bacteria in relying on the Sox system for the oxidation of reduced sulfur compounds. The genome has characteristics consistent with an obligately chemolithoautotrophic lifestyle, including few transporters predicted to have organic allocrits, and Calvin-Benson-Bassham cycle CDSs scattered throughout the genome.
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the genome of deep sea vent chemolithoautotroph thiomicrospira crunogena xcl 2
Lawrence Berkeley National Laboratory, 2006Co-Authors: Kathleen M Scott, Fereniki N Abril, Lois A Ball, Chantell J Barrett, Rodrigo A Blake, Amanda J Boller, Patrick S G Chain, Stefan M Sievert, Justine ClarkAbstract:Presented here is the complete genome sequence of Thiomicrospira crunogena XCL-2, representative of ubiquitous chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 bp), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. It has 14 Methyl-Accepting Chemotaxis Protein genes, including four that may assist in positioning it in the redoxcline. A relative abundance of CDSs encoding regulatory Proteins likely control the expression of genes encoding carboxysomes, multiple dissolved inorganic nitrogen and phosphate transporters, as well as a phosphonate operon, which provide this species with a variety of options for acquiring these substrates from the environment. T. crunogena XCL-2 is unusual among obligate sulfur oxidizing bacteria in relying on the Sox system for the oxidation of reduced sulfur compounds. A 38 kb prophage is present, and a high level of prophage induction was observed, which may play a role in keeping competing populations of close relatives in check. The genome has characteristics consistent with an obligately chemolithoautotrophic lifestyle, including few transporters predicted to have organic allocrits, and Calvin-Benson-Bassham cycle CDSs scattered throughout the genome.
Patrick S G Chain - One of the best experts on this subject based on the ideXlab platform.
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the genome of deep sea vent chemolithoautotroph thiomicrospira crunogena xcl 2
PLOS Biology, 2006Co-Authors: Kathleen M Scott, Fereniki N Abril, Lois A Ball, Chantell J Barrett, Rodrigo A Blake, Amanda J Boller, Patrick S G Chain, Stefan M Sievert, Justine ClarkAbstract:Presented here is the complete genome sequence of Thiomicrospira crunogena XCL-2, representative of ubiquitous chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 base pairs), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. It has 14 Methyl-Accepting Chemotaxis Protein genes, including four that may assist in positioning it in the redoxcline. A relative abundance of coding sequences (CDSs) encoding regulatory Proteins likely control the expression of genes encoding carboxysomes, multiple dissolved inorganic nitrogen and phosphate transporters, as well as a phosphonate operon, which provide this species with a variety of options for acquiring these substrates from the environment. Thiom. crunogena XCL-2 is unusual among obligate sulfur-oxidizing bacteria in relying on the Sox system for the oxidation of reduced sulfur compounds. The genome has characteristics consistent with an obligately chemolithoautotrophic lifestyle, including few transporters predicted to have organic allocrits, and Calvin-Benson-Bassham cycle CDSs scattered throughout the genome.
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the genome of deep sea vent chemolithoautotroph thiomicrospira crunogena xcl 2
Lawrence Berkeley National Laboratory, 2006Co-Authors: Kathleen M Scott, Fereniki N Abril, Lois A Ball, Chantell J Barrett, Rodrigo A Blake, Amanda J Boller, Patrick S G Chain, Stefan M Sievert, Justine ClarkAbstract:Presented here is the complete genome sequence of Thiomicrospira crunogena XCL-2, representative of ubiquitous chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 bp), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. It has 14 Methyl-Accepting Chemotaxis Protein genes, including four that may assist in positioning it in the redoxcline. A relative abundance of CDSs encoding regulatory Proteins likely control the expression of genes encoding carboxysomes, multiple dissolved inorganic nitrogen and phosphate transporters, as well as a phosphonate operon, which provide this species with a variety of options for acquiring these substrates from the environment. T. crunogena XCL-2 is unusual among obligate sulfur oxidizing bacteria in relying on the Sox system for the oxidation of reduced sulfur compounds. A 38 kb prophage is present, and a high level of prophage induction was observed, which may play a role in keeping competing populations of close relatives in check. The genome has characteristics consistent with an obligately chemolithoautotrophic lifestyle, including few transporters predicted to have organic allocrits, and Calvin-Benson-Bassham cycle CDSs scattered throughout the genome.
Kazuhiro Toyoda - One of the best experts on this subject based on the ideXlab platform.
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Cluster II che genes of Pseudomonas syringae pv. tabaci 6605, orthologs of cluster I in Pseudomonas aeruginosa, are required for Chemotaxis and virulence
Molecular Genetics and Genomics, 2021Co-Authors: Stephany Angelia Tumewu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Fumiko Taguchi, Yujiro Ogawa, Takumi Okamoto, Yuka Sugihara, Hajime Yamada, Kazuhiro ToyodaAbstract:Pseudomonas syringae pv. tabaci 6605 ( Pta 6605) is a causal agent of wildfire disease in host tobacco plants and is highly motile. Pta 6605 has multiple clusters of Chemotaxis genes including cheA , a gene encoding a histidine kinase, cheY , a gene encoding a response regulator, mcp, a gene for a Methyl-Accepting Chemotaxis Protein, as well as flagellar and pili biogenesis genes. However, only two major Chemotaxis gene clusters, cluster I and cluster II, possess cheA and cheY . Deletion mutants of cheA or cheY were constructed to evaluate their possible role in Pta 6605 Chemotaxis and virulence. Motility tests and a Chemotaxis assay to known attractant demonstrated that cheA2 and cheY2 mutants were unable to swarm and to perform Chemotaxis, whereas cheA1 and cheY1 mutants retained Chemotaxis ability almost equal to that of the wild-type (WT) strain. Although WT and cheY1 mutants of Pta 6605 caused severe disease symptoms on host tobacco leaves, the cheA2 and cheY2 mutants did not, and symptom development with cheA1 depended on the inoculation method. These results indicate that Chemotaxis genes located in cluster II are required for optimal Chemotaxis and host plant infection by Pta 6605 and that cluster I may partially contribute to these phenotypes.
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Vfr targets promoter of genes encoding Methyl-Accepting Chemotaxis Protein in Pseudomonas syringae pv. tabaci 6605.
Biochemistry and biophysics reports, 2021Co-Authors: Keisuke Ogura, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Fumiko Taguchi, Yuki IchinoseAbstract:Virulence factor regulator (Vfr) is an indispensable transcription factor in the expression of virulence in the phytopathogenic bacteria Pseudomonas syringae. However, the function of Vfr is not known so far. The deletion of vfr resulted in the loss of surface swarming motility and reduced the virulence in P. syringae pv. tabaci (Pta) 6605. In order to identify the target genes of Vfr, we screened the sequences that bind to Vfr by chromatin immune precipitation (ChIP) and sequencing methods using the closely related bacterium P. syringae pv. syringae (Pss) B728a. For this purpose we first generated a strain that possesses the recombinant gene vfr::FLAG in Pss B728a, and performed ChIP using an anti-FLAG antibody. Immunoprecipitated DNA was purified and sequenced with Illumina HiSeq. The Vfr::FLAG-specific peaks were further subjected to an electrophoresis mobility-shift assay, and the promoter regions of locus tag for Psyr_0578 , Psyr_1776, and Psyr_2237 were identified as putative target genes of Vfr. These genes encode plant pathogen-specific Methyl-Accepting Chemotaxis Proteins (Mcp). These mcp genes seem to be involved in the Vfr-regulated expression of virulence.
Judith P. Armitage - One of the best experts on this subject based on the ideXlab platform.
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Identification and localization of a methyl‐accepting Chemotaxis Protein in Rhodobacter sphaeroides
Molecular microbiology, 2002Co-Authors: George H. Wadhams, Angela Martin, Judith P. ArmitageAbstract:Genes coding for a classical membrane spanning chemoreceptor (mcpG) and a response regulator (cheY4) were identified in a region of Rhodobacter sphaeroides DNA unlinked to either of the two previously identified chemosensory operons. Immunogold electron microscopy had shown that the expression of chemoreceptors in R. sphaeroides varies with growth conditions. Using GFP fused to the newly identified McpG, we examined the targeting of this single Methyl-Accepting Chemotaxis Protein (MCP) under different growth conditions. The gene encoding the C-terminal McpG-GFP fusion was introduced by homologous recombination into the chromosome, replacing the wild-type gene. The resultant Protein localized to the poles of the cell under aerobic, photoheterotrophic and anaerobic dark conditions, demonstrating that this MCP is expressed under all three growth conditions. More Protein was always found at one pole than the other. The polar fluorescence increased during the cell cycle, with Protein becoming evident at the second pole around the time of septation. At division, each daughter cell had a label at one pole, but the intensity of fluorescence was higher in the daughter cell containing the original labelled pole. McpG localization was not altered in a che Operon 1 deletion strain, lacking CheW1 and CheA1, but a che Operon 2 deletion strain, lacking CheW2, CheW3 and CheA2, showed significantly reduced polar localization. This observation indicates that polar localization of McpG depends on Che Proteins encoded by Operon 2, but not homologues encoded by Operon 1.
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Identification of a Methyl-Accepting Chemotaxis Protein in Rhodobacter sphaeroides
Molecular microbiology, 1995Co-Authors: Mandy J. Ward, David M. Harrison, Martin J. Ebner, Judith P. ArmitageAbstract:Analysis of the DNA sequence directly upstream of the Chemotaxis operon of Rhodobacter sphaeroides identified a single gene whose product has strong similarity to the Methyl-Accepting Chemotaxis Proteins (MCPs) found in enteric bacteria. The deduced Protein had a highly conserved signalling sequence and only one very hydrophobic region at the N-terminus, in contrast to enteric MCPs. A possible cytoplasmic location of the majority of the Protein was supported by Western blotting. The mcpA gene was insertionally inactivated and the resulting phenotype examined using swarm plate assays. The mutant lacking McpA lost Chemotaxis to a wide range of attractant stimuli but only under aerobic conditions; it retained almost normal Chemotaxis under anaerobic/photosynthetic conditions. The identification of a sensory Protein which is active only under one set of growth conditions suggests that R. sphaeroides probably has several MCPs, which co-ordinately respond to changes in environmental conditions. Southern hybridization at relaxed stringency to the conserved sequence of the R. sphaeroides and Caulobacter crescentus mcp genes identified three possible additional mcp genes.
Kathleen M Scott - One of the best experts on this subject based on the ideXlab platform.
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the genome of deep sea vent chemolithoautotroph thiomicrospira crunogena xcl 2
PLOS Biology, 2006Co-Authors: Kathleen M Scott, Fereniki N Abril, Lois A Ball, Chantell J Barrett, Rodrigo A Blake, Amanda J Boller, Patrick S G Chain, Stefan M Sievert, Justine ClarkAbstract:Presented here is the complete genome sequence of Thiomicrospira crunogena XCL-2, representative of ubiquitous chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 base pairs), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. It has 14 Methyl-Accepting Chemotaxis Protein genes, including four that may assist in positioning it in the redoxcline. A relative abundance of coding sequences (CDSs) encoding regulatory Proteins likely control the expression of genes encoding carboxysomes, multiple dissolved inorganic nitrogen and phosphate transporters, as well as a phosphonate operon, which provide this species with a variety of options for acquiring these substrates from the environment. Thiom. crunogena XCL-2 is unusual among obligate sulfur-oxidizing bacteria in relying on the Sox system for the oxidation of reduced sulfur compounds. The genome has characteristics consistent with an obligately chemolithoautotrophic lifestyle, including few transporters predicted to have organic allocrits, and Calvin-Benson-Bassham cycle CDSs scattered throughout the genome.
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the genome of deep sea vent chemolithoautotroph thiomicrospira crunogena xcl 2
Lawrence Berkeley National Laboratory, 2006Co-Authors: Kathleen M Scott, Fereniki N Abril, Lois A Ball, Chantell J Barrett, Rodrigo A Blake, Amanda J Boller, Patrick S G Chain, Stefan M Sievert, Justine ClarkAbstract:Presented here is the complete genome sequence of Thiomicrospira crunogena XCL-2, representative of ubiquitous chemolithoautotrophic sulfur-oxidizing bacteria isolated from deep-sea hydrothermal vents. This gammaproteobacterium has a single chromosome (2,427,734 bp), and its genome illustrates many of the adaptations that have enabled it to thrive at vents globally. It has 14 Methyl-Accepting Chemotaxis Protein genes, including four that may assist in positioning it in the redoxcline. A relative abundance of CDSs encoding regulatory Proteins likely control the expression of genes encoding carboxysomes, multiple dissolved inorganic nitrogen and phosphate transporters, as well as a phosphonate operon, which provide this species with a variety of options for acquiring these substrates from the environment. T. crunogena XCL-2 is unusual among obligate sulfur oxidizing bacteria in relying on the Sox system for the oxidation of reduced sulfur compounds. A 38 kb prophage is present, and a high level of prophage induction was observed, which may play a role in keeping competing populations of close relatives in check. The genome has characteristics consistent with an obligately chemolithoautotrophic lifestyle, including few transporters predicted to have organic allocrits, and Calvin-Benson-Bassham cycle CDSs scattered throughout the genome.