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Jinxin Li - One of the best experts on this subject based on the ideXlab platform.
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lsp1 a responsive protein from Meyerozyma guilliermondii elicits defence response and improves glycyrrhizic acid biosynthesis in glycyrrhiza uralensis fisch adventitious roots
Journal of Cellular Physiology, 2017Co-Authors: Juan Wang, Jianli Li, Jing Li, Jinxin LiAbstract:This research explored the effects of protein and polysaccharide in Meyerozyma guilliermondii on active compounds in Glycyrrhiza uralensis Fisch adventitious roots. In this study, a responsive protein LSP1 was purified from the Meyerozyma guilliermondii since the excellent induction. The contents of total flavonoids (3.46 mg · g−1), glycyrrhizic acid (0.41 mg · g−1), glycyrrhetinic acid (0.41 mg · g−1) and polysaccharide (94.49 mg · g−1) in adventitious root peaked at LSP1 group, which were 1.6, 3.4, 2.4, 2.0-fold that of control, respectively. Besides, the responsive protein LSP1 significantly activated the defense signaling, mitogen-activated protein kinases and extremely up-regulated the expression of defense-related genes and functional genes involved in glycyrrhizic acid biosynthesis. This article is protected by copyright. All rights reserved
Zhen-ming Chi - One of the best experts on this subject based on the ideXlab platform.
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Enhanced citric acid production by a yeast Yarrowia lipolytica over-expressing a pyruvate carboxylase gene.
Bioprocess and biosystems engineering, 2016Co-Authors: Mei-juan Tan, Guang-lei Liu, Xi Chen, Yu-kuan Wang, Zhen-ming ChiAbstract:In this study, after the expression of a pyruvate carboxylase gene (PYC) cloned from Meyerozyma guilliermondii in a marine-derived yeast Yarrowia lipolytica SWJ-1b, a transformant PG86 obtained had much higher PYC activity than Y. lipolytica SWJ-1b. At the same time, the PYC gene expression and citric acid (CA) production by the transformant PG86 were also greatly enhanced. When glucose concentration in the medium was 60.0 g L−1, CA concentration formed by the transformant PG86 was 34.02 g L−1, leading to a CA yield of 0.57 g g−1 of glucose. During a 10-L fed-batch fermentation, the final concentration of CA was 101.0 ± 1.3 g L−1, the yield was 0.89 g g−1 of glucose, the productivity was 0.42 g L−1 h−1 and only 5.93 g L−1 reducing sugar was left in the fermented medium within 240 h of the fed-batch fermentation. HPLC analysis showed that most of the fermentation products were CA.
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Enhanced expression of the codon-optimized exo-inulinase gene from the yeast Meyerozyma guilliermondii in Saccharomyces sp. W0 and bioethanol production from inulin.
Applied microbiology and biotechnology, 2014Co-Authors: Guang-lei Liu, Zhe Chi, Zhen-ming ChiAbstract:In the present study, after the exo-inulinase gene INU1 from Meyerozyma guilliermondii was optimized according to the codon usage bias of Saccharomyces cerevisiae, both the optimized gene INU1Y and the native gene INU1 were ligated into the homologous integration expression vector pMIRSC11 and expressed in Saccharomyces sp. W0. It was determined that the inulinase activity of the recombinant yeast Y13 with the optimized gene INU1Y was 43.84 U/mL, which was obviously higher than that (31.39 U/mL) produced by the recombinant yeast EX3 with the native gene INU1. Moreover, it was indicated that the recombinant yeast Y13 could produce 126.30 mg/mL ethanol from 300.0 g/L inulin while the recombinant yeast EX3 and Saccharomyces sp. W0 produced 122.75 mg/mL and 114.15 mg/mL ethanol, respectively, under the same conditions. In addition, the ethanol productivity of the recombinant yeast Y13 was 2.25 mg/mL/h within 48 h of the fermentation, which was obviously higher than that of the recombinant yeast EX3 (1.97 mg/mL/h) and Saccharomyces sp. W0 (1.77 mg/mL/h) within the same period. The results demonstrated that the recombinant yeast Y13 had higher ethanol production and productivity than the recombinant yeast EX3 and Saccharomyces sp. W0. Therefore, it was concluded that the codon optimization of the exo-inulinase gene from M. guilliermondii effectively enhanced inulinase activity and improved ethanol production from inulin by Saccharomyces sp. W0 carrying the optimized inulinase gene.
Hongyin Zhang - One of the best experts on this subject based on the ideXlab platform.
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Protein Expression Profile and Transcriptome Characterization of Penicillium expansum Induced by Meyerozyma guilliermondii
Journal of Food Quality, 2020Co-Authors: Qiya Yang, Dhanasekaran Solairaj, Maurice Tibiru Apaliya, Mandour H. Abdelhai, Marui Zhu, Yuan Yan, Hongyin ZhangAbstract:Antagonistic yeasts can inhibit fungal growth. In our previous research, Meyerozyma guilliermondii, one of the antagonistic yeasts, exhibited antagonistic activity against Penicillium expansum. However, the mechanisms, especially the molecular mechanisms of inhibiting activity of M. guilliermondii, are not clear. In this study, the protein expression profile and transcriptome characterization of P. expansum induced by M. guilliermondii were investigated. In P. expansum induced by M. guilliermondii, 66 proteins were identified as differentially expressed, among them six proteins were upregulated and 60 proteins were downregulated, which were associated with oxidative phosphorylation, ATP synthesis, basal metabolism, and response regulation. Simultaneously, a transcriptomic approach based on RNA-Seq was applied to annotate the genome of P. expansum and then studied the changes of gene expression in P. expansum treated with M. guilliermondii. The results showed that differentially expressed genes such as HEAT, Phosphoesterase, Polyketide synthase, ATPase, and Ras-association were significantly downregulated, in contrast to Cytochromes P450, Phosphatidate cytidylyltransferase, and Glutathione S-transferase, which were significantly upregulated. Interestingly, the downregulated differentially expressed proteins and genes have a corresponding relationship; these results revealed that these proteins and genes were important in the growth of P. expansum treated with M. guilliermondii.
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Investigating the effect of methyl jasmonate on the biocontrol activity of Meyerozyma guilliermondii against blue mold decay of apples and the possible mechanisms involved
Physiological and Molecular Plant Pathology, 2020Co-Authors: Lina Zhao, Mandour H. Abdelhai, Xiangfeng Zheng, Nana Adwoa Serwah Boateng, Xuhua Zhang, Hongyin ZhangAbstract:Abstract Apple is one of the largely grown fruits in China, and it suffers great economic losses caused by Penicillium expansum infection every year. In the present study, we ascertained the efficacy of Meyerozyma guilliermondii enhanced by 200 μmol/L methyl jasmonate (MeJA) in controlling the postharvest blue mold decay of apples and the possible mechanisms involved. The results demonstrated that the biological control ability of M. guilliermondii induced by MeJA in restraining P. expansum infection in apples. The efficacy of M. guilliermondii induced by MeJA significantly decreased the decay incidence and lesion diameter of apples. When treated with M. guilliermondii induced by MeJA, the decay incidence of apples was only 21.6%, while apples treated with M. guilliermondii was 42.4%. M. guilliermondii induced by MeJA also reduced the germ tube length, spore germination rate of P. expansum in PDB and colony diameter of P. expansum in PDA. The results also showed that antagonistic yeast induced by MeJA proliferated better in apple wounds and surface at 4 °C or 20 °C. Meanwhile, the activities of resistance-related peroxidase (POD), polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), Catalase (CAT), and the antibacterial substances of flavonoid contents, total phenolic content were all increased. The results manifested that M. guilliermondii induced by MeJA increased the expression levels of defense-related enzymes in apples.
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Transcriptome characterization and expression profile of defense-related genes in pear induced by Meyerozyma guilliermondii
Postharvest Biology and Technology, 2018Co-Authors: Yuan Yan, Maurice Tibiru Apaliya, Xiangfeng Zheng, Hongjuan Yang, Hongyin ZhangAbstract:Abstract Meyerozyma guilliermondii significantly inhibited natural decay of stored pears without adverse effects on storage qualities and induced resistance of pears. It was noticed that M. guilliermondii had a significant effect on the induction of several defense-related genes compared to the control, such as genes coding for phenylalanine ammonia lyase (PAL), peroxidase(POD)and β-1, 3 glucannase (GLU). Furthermore, the expression level of GLU in pears treated with M. guilliermondii increased 105-fold compared to the control at 0 d. These findings indicated that M. guilliermondii enhanced the defense-related mechanism of pears. The transcriptome of pears treated with M. guilliermondii and the control were explored after 3 days, the result showed that 144 genes (Log2 fold change ≥ 2, FDR ＜ 0.05) were significantly up-regulated by the induction of M. guilliermondii, some of which include genes of defense-related enzymes such as G-protein coupled receptor 1-like, cationic peroxidase 1-like and beta-glucosidase 12-like were induced corresponding to PAL, POD and GLU respectively. Defense-related transcription factors such as WRKY9, WRKY31 and some other pathogenesis-related genes like Major allergen Pyr c 1, major allergen Pru ar 1-like and major allergen Pru av 1-like were also induced. These results provided a new insight into the biocontrol mechanism of the antagonist yeast in pears.
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Control of postharvest blue mold decay in pears by Meyerozyma guilliermondii and it’s effects on the protein expression profile of pears
Postharvest Biology and Technology, 2018Co-Authors: Yuan Yan, Qiya Yang, Maurice Tibiru Apaliya, Xiangfeng Zheng, Xiaoyun Zhang, Lina Zhao, Hongyin ZhangAbstract:Abstract This study assessed the biocontrol efficacy of Meyerozyma guilliermondii against blue mold decay caused by Penicillium expansum in pears and the possible mechanisms involved. The results indicated that M. guilliermondii significantly inhibited the blue mold decay caused by P. expansum without affecting the quality of the pears. M. guilliermondii rapidly colonized the wounds and surfaces of the pears at both 4 °C and 20 °C. The rapid growth in the population of M. guilliermondii in the wounds and surface environments of pears indicated that it has the potential to inhibit pathogens in pears. The activities of antioxidant enzymes (peroxidase and catalase) in the pear were improved after the application of the yeast. Phenylalanine ammonialyase (PAL), a key enzyme involved in lignin biosynthesis and defense related activity, was also markedly enhanced. Generally, the application of yeast induced disease resistance in the pear. The results pear proteomics profile after M. guilliermondii treatment showed that 17 proteins were significantly up-regulated and 13 were down-regulated in response to induction with M. guilliermondii. Most of the proteins were involved in defense and stress responses based on biological process. These results provided a new insight into the biocontrol mechanism of the antagonist yeast in the pear fruit.
Juan Wang - One of the best experts on this subject based on the ideXlab platform.
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Gene expression of glycyrrhizin acid and accumulation of endogenous signaling molecule in Glycyrrhiza uralensis Fisch adventitious roots after Saccharomyces cerevisiae and Meyerozyma guilliermondii applications.
Biotechnology and applied biochemistry, 2017Co-Authors: Shujie Liu, Juan Wang, Wenyuan GaoAbstract:This study reports the best culture conditions for roots growth and accumulation of active components by optimizing the parameters. Glycyrrhiza uralensis adventitious roots metabolites were significantly increased after adding Saccharomyces cerevisiae and Meyerozyma guilliermondii. The highest contents of polysaccharide, glycyrrhizic acid, glycyrrhetinic acid, and total flavonoids were obtained in M. guilliermondii group; the content of glycyrrhizic acid was 5.3-fold higher than the control. In control and treatment groups, 12 compounds were identified by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS), among which some new compounds have been detected in elicitor groups including 5,7-dihydroxyflavanone, glycyrrhisoflavanone, licorice saponin J2, uralsaponin B, (3R)-vestitol, and uralenol. Meyerozyma guilliermondii significantly upregulated the expression of the genes such as 3-hydroxy-3-methylglutaryl coenzyme A reductase, farnesyl diphosphate synthase, geranyl diphosphate synthase, squalene synthase, squalene epoxidase, β-amyrin synthase, and CYP88D6 and CYP72A154. Meanwhile, it increased the biosynthesis of signaling molecules (nitric oxide, salicylic acid, and jasmonic acid) in defense mechanism.
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lsp1 a responsive protein from Meyerozyma guilliermondii elicits defence response and improves glycyrrhizic acid biosynthesis in glycyrrhiza uralensis fisch adventitious roots
Journal of Cellular Physiology, 2017Co-Authors: Juan Wang, Jianli Li, Jing Li, Jinxin LiAbstract:This research explored the effects of protein and polysaccharide in Meyerozyma guilliermondii on active compounds in Glycyrrhiza uralensis Fisch adventitious roots. In this study, a responsive protein LSP1 was purified from the Meyerozyma guilliermondii since the excellent induction. The contents of total flavonoids (3.46 mg · g−1), glycyrrhizic acid (0.41 mg · g−1), glycyrrhetinic acid (0.41 mg · g−1) and polysaccharide (94.49 mg · g−1) in adventitious root peaked at LSP1 group, which were 1.6, 3.4, 2.4, 2.0-fold that of control, respectively. Besides, the responsive protein LSP1 significantly activated the defense signaling, mitogen-activated protein kinases and extremely up-regulated the expression of defense-related genes and functional genes involved in glycyrrhizic acid biosynthesis. This article is protected by copyright. All rights reserved
Siti Nurbaya Oslan - One of the best experts on this subject based on the ideXlab platform.
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A Host-Vector System for the Expression of a Thermostable Bacterial Lipase in a Locally Isolated Meyerozyma guilliermondii SMB.
Microorganisms, 2020Co-Authors: Abu Bakar Salleh, Siti Marha Baharuddin, Raja Noor Zaliha Raja Abd Rahman, Thean Chor Leow, Mahiran Basri, Siti Nurbaya OslanAbstract:Screening for a new yeast as an alternative host is expected to solve the limitations in the present yeast expression system. A yeast sample which was isolated from the traditional food starter ‘ragi’ from Malaysia was identified to contain Meyerozyma guilliermondii strain SMB. This yeast-like fungus strain SMB was characterized to assess its suitability as an expression host. Lipase activity was absent in this host (when assayed at 30 °C and 70 °C) and Hygromycin B (50 μg/mL) was found to be its best selection marker. Then, the hyg gene (Hygromycin B) was used to replace the sh ble gene (Zeocin) expression cassette in a Komagataella phaffii expression vector (designated as pFLDhα). A gene encoding the mature thermostable lipase from Bacillus sp. L2 was cloned into pFLDhα, followed by transformation into strain SMB. The optimal expression of L2 lipase was achieved using YPTM (Yeast Extract-Peptone-Tryptic-Methanol) medium after 48 h with 0.5% (v/v) methanol induction, which was 3 times faster than another K. phaffii expression system. In conclusion, a new host-vector system was established as a platform to express L2 lipase under the regulation of PFLD1. It could also be promising to express other recombinant proteins without inducers.
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Structure Prediction of a Thermostable SR74 α-Amylase from Geobacillus stearothermophilus Expressed in CTG-Clade Yeast Meyerozyma guilliermondii Strain SO
Catalysts, 2020Co-Authors: Si Lim, Abu Bakar Salleh, Noor Dina Muhd Noor, Siti Nurbaya OslanAbstract:α-amylase which catalyzes the hydrolysis of α-1,4-glycosidic bonds in starch have frequently been cloned into various microbial workhorses to yield a higher recombinant titer. A thermostable SR74 α-amylase from Geobacillus stearothermophilus was found to have a huge potential in detergent industries due to its thermostability properties. The gene was cloned into a CTG-clade yeast Meyerozyma guilliermondii strain SO. However, the CUG ambiguity present in the strain SO has possibly altered the amino acid residues in SR74 amylase wild type (WT) encoded by CUG the codon from the leucine to serine. From the multiple sequence alignment, six mutations were found in recombinant SR74 α-amylase (rc). Their effects on SR74 α-amylase structure and function remain unknown. Herein, we predicted the structures of the SR74 amylases (WT and rc) using the template 6ag0.1.A (PDB ID: 6ag0). We sought to decipher the possible effects of CUG ambiguity in strain SO via in silico analysis. They are structurally identical, and the metal triad (CaI–CaIII) might contribute to the thermostability while CaIV was attributed to substrate specificity. Since the pairwise root mean square deviation (RMSD) between the WT and rc SR74 α-amylase was lower than the template, we suggest that the biochemical properties of rc SR74 α-amylase were better deduced from its WT, especially its thermostability.
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Molecular Expression of a Recombinant Thermostable Bacterial Amylase from Geobacillus stearothermophilus SR74 Using Methanol-free Meyerozyma guilliermondii Strain SO Yeast System
Bioresources, 2020Co-Authors: Nurul Syazwani Mohamad Nasir, Abu Bakar Salleh, Chor Thean Leow, Siti Nur Hazwani Oslan, Siti Nurbaya OslanAbstract:α-Amylase, which was isolated from Geobacillus stearothermophilus SR74, has shown its potential to be used in industrial applications. However, its expression in the Pichia pastoris expression system with the alcohol oxidase 1 promoter (PAOX1) requires high methanol consumption and is time-consuming. This study aimed to express SR74 α-amylase in an alternative yeast system, using Meyerozyma guilliermondii strain SO, which was isolated from a spoiled orange (SO) under the regulation of a formaldehyde dehydrogenase promoter (PFLD). Qualitative screening showed that strain SO possessed a native amylase grown on YPD-starch plate at 30 °C. The recombinant SR74 α-amylase was further quantified and validated using the Western blot test. It was confirmed that SR74 α-amylase was expressed by strain SO extracellularly with a size of 59 kDa. Optimization in a shake flask showed that the recombinant SR74 α-amylase, which was regulated by PFLD, was successfully produced (26 U/mL) without any external inducer in the YPT medium after 24 h of cultivation. In conclusion, strain SO was able to produce SR74 amylase without methanol in one-fifth the fermentation time of P. pastoris. Further optimization of the expression may be done to improve the yield, as this methanol-free host is still underexplored.