The Experts below are selected from a list of 23442 Experts worldwide ranked by ideXlab platform

H Horn - One of the best experts on this subject based on the ideXlab platform.

  • structure and shear strength of Microbial Biofilms as determined with confocal laser scanning microscopy and fluid dynamic gauging using a novel rotating disc biofilm reactor
    Biotechnology and Bioengineering, 2007
    Co-Authors: Roland B Mohle, Timo Langemann, M Haesner, Wolfgang Augustin, Stephan Scholl, Dietmar C Hempel, H Horn
    Abstract:

    The cohesive strength of Microbial Biofilms cultivated on a rotating disc has been measured using fluid dynamic gauging (FDG). The thickness of heterotrophic mixed culture Biofilms was found to depend on substrate concentration and shear force at the biofilm surface during the cultivation. For high substrate concentrations and low shear forces the biofilm thickness increased to several 100 µm within 7 days. Low substrate concentration and higher shear forces yielded thin Biofilms of about 100 µm thickness. Independent from cultivation conditions and thickness of the Biofilms their cohesive strength ranged between 6.0 and 7.7 N m−2. The ratio between cohesive strength measured with FDG and shear forces applied during biofilm cultivation have ranged from 200 to 1,100. Higher concentrations of iron in the cultivation media has a positive effect on the stability of the Biofilms cultivated. By using the CLSM technique a stable base biofilm with a high amount of stained EPS glycoconjugates could be visualized after gauging. The thickness of the base biofilm was about 100 µm for all Biofilms cultivated and was not removable under the applied shear conditions used during FDG. Biotechnol. Bioeng. 2007;98: 747–755. © 2007 Wiley Periodicals, Inc.

  • structure and shear strength of Microbial Biofilms as determined with confocal laser scanning microscopy and fluid dynamic gauging using a novel rotating disc biofilm reactor
    Biotechnology and Bioengineering, 2007
    Co-Authors: Roland B Mohle, Timo Langemann, M Haesner, Wolfgang Augustin, Stephan Scholl, Dietmar C Hempel, Thomas R Neu, H Horn
    Abstract:

    The cohesive strength of Microbial Biofilms cultivated on a rotating disc has been measured using fluid dynamic gauging (FDG). The thickness of heterotrophic mixed culture Biofilms was found to depend on substrate concentration and shear force at the biofilm surface during the cultivation. For high substrate concentrations and low shear forces the biofilm thickness increased to several 100 microm within 7 days. Low substrate concentration and higher shear forces yielded thin Biofilms of about 100 microm thickness. Independent from cultivation conditions and thickness of the Biofilms their cohesive strength ranged between 6.0 and 7.7 N m(-2). The ratio between cohesive strength measured with FDG and shear forces applied during biofilm cultivation have ranged from 200 to 1,100. Higher concentrations of iron in the cultivation media has a positive effect on the stability of the Biofilms cultivated. By using the CLSM technique a stable base biofilm with a high amount of stained EPS glycoconjugates could be visualized after gauging. The thickness of the base biofilm was about 100 microm for all Biofilms cultivated and was not removable under the applied shear conditions used during FDG.

M Haesner - One of the best experts on this subject based on the ideXlab platform.

  • structure and shear strength of Microbial Biofilms as determined with confocal laser scanning microscopy and fluid dynamic gauging using a novel rotating disc biofilm reactor
    Biotechnology and Bioengineering, 2007
    Co-Authors: Roland B Mohle, Timo Langemann, M Haesner, Wolfgang Augustin, Stephan Scholl, Dietmar C Hempel, H Horn
    Abstract:

    The cohesive strength of Microbial Biofilms cultivated on a rotating disc has been measured using fluid dynamic gauging (FDG). The thickness of heterotrophic mixed culture Biofilms was found to depend on substrate concentration and shear force at the biofilm surface during the cultivation. For high substrate concentrations and low shear forces the biofilm thickness increased to several 100 µm within 7 days. Low substrate concentration and higher shear forces yielded thin Biofilms of about 100 µm thickness. Independent from cultivation conditions and thickness of the Biofilms their cohesive strength ranged between 6.0 and 7.7 N m−2. The ratio between cohesive strength measured with FDG and shear forces applied during biofilm cultivation have ranged from 200 to 1,100. Higher concentrations of iron in the cultivation media has a positive effect on the stability of the Biofilms cultivated. By using the CLSM technique a stable base biofilm with a high amount of stained EPS glycoconjugates could be visualized after gauging. The thickness of the base biofilm was about 100 µm for all Biofilms cultivated and was not removable under the applied shear conditions used during FDG. Biotechnol. Bioeng. 2007;98: 747–755. © 2007 Wiley Periodicals, Inc.

  • structure and shear strength of Microbial Biofilms as determined with confocal laser scanning microscopy and fluid dynamic gauging using a novel rotating disc biofilm reactor
    Biotechnology and Bioengineering, 2007
    Co-Authors: Roland B Mohle, Timo Langemann, M Haesner, Wolfgang Augustin, Stephan Scholl, Dietmar C Hempel, Thomas R Neu, H Horn
    Abstract:

    The cohesive strength of Microbial Biofilms cultivated on a rotating disc has been measured using fluid dynamic gauging (FDG). The thickness of heterotrophic mixed culture Biofilms was found to depend on substrate concentration and shear force at the biofilm surface during the cultivation. For high substrate concentrations and low shear forces the biofilm thickness increased to several 100 microm within 7 days. Low substrate concentration and higher shear forces yielded thin Biofilms of about 100 microm thickness. Independent from cultivation conditions and thickness of the Biofilms their cohesive strength ranged between 6.0 and 7.7 N m(-2). The ratio between cohesive strength measured with FDG and shear forces applied during biofilm cultivation have ranged from 200 to 1,100. Higher concentrations of iron in the cultivation media has a positive effect on the stability of the Biofilms cultivated. By using the CLSM technique a stable base biofilm with a high amount of stained EPS glycoconjugates could be visualized after gauging. The thickness of the base biofilm was about 100 microm for all Biofilms cultivated and was not removable under the applied shear conditions used during FDG.

Roland B Mohle - One of the best experts on this subject based on the ideXlab platform.

  • structure and shear strength of Microbial Biofilms as determined with confocal laser scanning microscopy and fluid dynamic gauging using a novel rotating disc biofilm reactor
    Biotechnology and Bioengineering, 2007
    Co-Authors: Roland B Mohle, Timo Langemann, M Haesner, Wolfgang Augustin, Stephan Scholl, Dietmar C Hempel, H Horn
    Abstract:

    The cohesive strength of Microbial Biofilms cultivated on a rotating disc has been measured using fluid dynamic gauging (FDG). The thickness of heterotrophic mixed culture Biofilms was found to depend on substrate concentration and shear force at the biofilm surface during the cultivation. For high substrate concentrations and low shear forces the biofilm thickness increased to several 100 µm within 7 days. Low substrate concentration and higher shear forces yielded thin Biofilms of about 100 µm thickness. Independent from cultivation conditions and thickness of the Biofilms their cohesive strength ranged between 6.0 and 7.7 N m−2. The ratio between cohesive strength measured with FDG and shear forces applied during biofilm cultivation have ranged from 200 to 1,100. Higher concentrations of iron in the cultivation media has a positive effect on the stability of the Biofilms cultivated. By using the CLSM technique a stable base biofilm with a high amount of stained EPS glycoconjugates could be visualized after gauging. The thickness of the base biofilm was about 100 µm for all Biofilms cultivated and was not removable under the applied shear conditions used during FDG. Biotechnol. Bioeng. 2007;98: 747–755. © 2007 Wiley Periodicals, Inc.

  • structure and shear strength of Microbial Biofilms as determined with confocal laser scanning microscopy and fluid dynamic gauging using a novel rotating disc biofilm reactor
    Biotechnology and Bioengineering, 2007
    Co-Authors: Roland B Mohle, Timo Langemann, M Haesner, Wolfgang Augustin, Stephan Scholl, Dietmar C Hempel, Thomas R Neu, H Horn
    Abstract:

    The cohesive strength of Microbial Biofilms cultivated on a rotating disc has been measured using fluid dynamic gauging (FDG). The thickness of heterotrophic mixed culture Biofilms was found to depend on substrate concentration and shear force at the biofilm surface during the cultivation. For high substrate concentrations and low shear forces the biofilm thickness increased to several 100 microm within 7 days. Low substrate concentration and higher shear forces yielded thin Biofilms of about 100 microm thickness. Independent from cultivation conditions and thickness of the Biofilms their cohesive strength ranged between 6.0 and 7.7 N m(-2). The ratio between cohesive strength measured with FDG and shear forces applied during biofilm cultivation have ranged from 200 to 1,100. Higher concentrations of iron in the cultivation media has a positive effect on the stability of the Biofilms cultivated. By using the CLSM technique a stable base biofilm with a high amount of stained EPS glycoconjugates could be visualized after gauging. The thickness of the base biofilm was about 100 microm for all Biofilms cultivated and was not removable under the applied shear conditions used during FDG.

Guillermo Diazpulido - One of the best experts on this subject based on the ideXlab platform.

  • coralline algal metabolites induce settlement and mediate the inductive effect of epiphytic microbes on coral larvae
    Scientific Reports, 2018
    Co-Authors: Luis A Gomezlemos, Christopher Doropoulos, Elisa Bayraktarov, Guillermo Diazpulido
    Abstract:

    Settlement of invertebrates is a key process affecting the structure of marine communities and underpins the ability of benthic ecosystems to recover from disturbance. While it is known that specific crustose coralline algae (CCA) are important for settlement of some coral species, the role of algal chemical compounds versus surface Microbial Biofilms has long been ambiguous. Using a model system - a CCA of a genus that has been shown to induce high levels of settlement of Acropora corals (Titanoderma cf. tessellatum) and an abundant coral species (Acropora millepora)- we show that chemical effects of CCA are stronger than those from CCA surface Microbial Biofilms as drivers of coral settlement. Biofilms contributed to some extent to larval settlement via synergistic effects, where Microbial cues were dependent on the CCA primary metabolism (production of dissolved organic carbon). We propose that optimal coral settlement is caused by complex biochemical communications among CCA, their epiphytic Microbial community and coral larvae.

Thomas R Neu - One of the best experts on this subject based on the ideXlab platform.

  • structure and shear strength of Microbial Biofilms as determined with confocal laser scanning microscopy and fluid dynamic gauging using a novel rotating disc biofilm reactor
    Biotechnology and Bioengineering, 2007
    Co-Authors: Roland B Mohle, Timo Langemann, M Haesner, Wolfgang Augustin, Stephan Scholl, Dietmar C Hempel, Thomas R Neu, H Horn
    Abstract:

    The cohesive strength of Microbial Biofilms cultivated on a rotating disc has been measured using fluid dynamic gauging (FDG). The thickness of heterotrophic mixed culture Biofilms was found to depend on substrate concentration and shear force at the biofilm surface during the cultivation. For high substrate concentrations and low shear forces the biofilm thickness increased to several 100 microm within 7 days. Low substrate concentration and higher shear forces yielded thin Biofilms of about 100 microm thickness. Independent from cultivation conditions and thickness of the Biofilms their cohesive strength ranged between 6.0 and 7.7 N m(-2). The ratio between cohesive strength measured with FDG and shear forces applied during biofilm cultivation have ranged from 200 to 1,100. Higher concentrations of iron in the cultivation media has a positive effect on the stability of the Biofilms cultivated. By using the CLSM technique a stable base biofilm with a high amount of stained EPS glycoconjugates could be visualized after gauging. The thickness of the base biofilm was about 100 microm for all Biofilms cultivated and was not removable under the applied shear conditions used during FDG.

  • Microbial extracellular polymeric substances characterization structure and function
    1999
    Co-Authors: Jost Wingender, Thomas R Neu, Hanscurt Flemming
    Abstract:

    Introduction What are Bacterial Extracellular Polymeric Substances?- In Situ Characterization of Extracellular Polymeric Substances (EPS) in Biofilm Systems Extraction of EPS Biofilm Exopolysaccharides Regulation of Matrix Polymer in Biofilm Formation and Dispersion Exopolymers of Sulphate-Reducing Bacteria Analysis and Function of the EPS from the Strong Acidophile Thiobacillus ferrooxidans Physical and Chemical Properties of Extracellular Polysaccharides Associated with Biofilms and Related Systems Chemical Communication Within Microbial Biofilms: Chemotaxis and Quorum Sensing in Bacterial Cells Function of EPS Polysaccharases in Biofilms - Sources - Action - Consequences!- Extracellular Enzymes Within Microbial Biofilms and the Role of the Extracellular Polymer Matrix Interaction Between Extracellular Polysaccharides and Enzymes.

  • Application of multiple parameter imaging for the quantification of algal, bacterial and exopolymer components of Microbial Biofilms
    Journal of Microbiological Methods, 1998
    Co-Authors: John R. Lawrence, Thomas R Neu, George D.w. Swerhone
    Abstract:

    Abstract Techniques are required for the simultaneous or sequential determination of multiple parameters within Microbial Biofilms. Confocal scanning laser microscopy in combination with a range of fluorescent probes and markers offers an approach to quantitatively defining many aspects of biofilm communities. By applying multispectral imaging in conjunction with nucleic acid stains, fluor conjugated lectins, and autofluorescence we have developed a simple approach to evaluate biofilm community composition. Biofilms were treated with the fluorescent nucleic acid stain SYTO 9 to allow quantification of bacterial biomass and fluor conjugated lectins (i.e., Triticum vulgaris lectin) to identify and allow quantification of exopolymeric substances. Far red autofluorescence was imaged to quantify algal biomass. Digital image analysis of the CSLM optical thin sections in each of the channels was used to determine such parameters as biofilm depth, bacterial cell area (biomass), exopolymer area and algal biomass at various depths and locations. In addition, three colour red–green–blue projections of the Biofilms were computed. The method proved simple and effective for determining treatment effects such as grazing by invertebrates.