The Experts below are selected from a list of 51 Experts worldwide ranked by ideXlab platform

Kenneth Hensley - One of the best experts on this subject based on the ideXlab platform.

  • a comparative review of Cell Culture systems for the study of Microglial biology in alzheimer s disease
    Journal of Neuroinflammation, 2012
    Co-Authors: Branden Stansley, J A Post, Kenneth Hensley
    Abstract:

    Over the past two decades, it has become increasingly apparent that Alzheimer’s disease neuropathology is characterized by activated microglia (brain resident macrophages) as well as the classic features of amyloid plaques and neurofibrillary tangles. The intricacy of Microglial biology has also become apparent, leading to a heightened research interest in this particular Cell type. Over the years a number of different Microglial Cell culturing techniques have been developed to study either primary mammalian microglia, or immortalized Cell lines. Each Microglial system has advantages and disadvantages and should be selected for its appropriateness in a particular research context. This review summarizes several of the most common Microglial Cell Culture systems currently being employed in Alzheimer’s research including primary microglia; BV2 and N9 retroviral immortalized microglia; human immortalized microglia (HMO6); and spontaneously immortalized rodent Microglial lines (EOC lines and HAPI Cells). Particularities of Cell Culture requirements and characteristics of Microglial behavior, especially in response to applied inflammogen stimuli, are compared and discussed across these Cell types.

  • A comparative review of Cell Culture systems for the study of Microglial biology in Alzheimer’s disease
    Journal of Neuroinflammation, 2012
    Co-Authors: Branden Stansley, J A Post, Kenneth Hensley
    Abstract:

    Over the past two decades, it has become increasingly apparent that Alzheimer’s disease neuropathology is characterized by activated microglia (brain resident macrophages) as well as the classic features of amyloid plaques and neurofibrillary tangles. The intricacy of Microglial biology has also become apparent, leading to a heightened research interest in this particular Cell type. Over the years a number of different Microglial Cell culturing techniques have been developed to study either primary mammalian microglia, or immortalized Cell lines. Each Microglial system has advantages and disadvantages and should be selected for its appropriateness in a particular research context. This review summarizes several of the most common Microglial Cell Culture systems currently being employed in Alzheimer’s research including primary microglia; BV2 and N9 retroviral immortalized microglia; human immortalized microglia (HMO6); and spontaneously immortalized rodent Microglial lines (EOC lines and HAPI Cells). Particularities of Cell Culture requirements and characteristics of Microglial behavior, especially in response to applied inflammogen stimuli, are compared and discussed across these Cell types.

Branden Stansley - One of the best experts on this subject based on the ideXlab platform.

  • a comparative review of Cell Culture systems for the study of Microglial biology in alzheimer s disease
    Journal of Neuroinflammation, 2012
    Co-Authors: Branden Stansley, J A Post, Kenneth Hensley
    Abstract:

    Over the past two decades, it has become increasingly apparent that Alzheimer’s disease neuropathology is characterized by activated microglia (brain resident macrophages) as well as the classic features of amyloid plaques and neurofibrillary tangles. The intricacy of Microglial biology has also become apparent, leading to a heightened research interest in this particular Cell type. Over the years a number of different Microglial Cell culturing techniques have been developed to study either primary mammalian microglia, or immortalized Cell lines. Each Microglial system has advantages and disadvantages and should be selected for its appropriateness in a particular research context. This review summarizes several of the most common Microglial Cell Culture systems currently being employed in Alzheimer’s research including primary microglia; BV2 and N9 retroviral immortalized microglia; human immortalized microglia (HMO6); and spontaneously immortalized rodent Microglial lines (EOC lines and HAPI Cells). Particularities of Cell Culture requirements and characteristics of Microglial behavior, especially in response to applied inflammogen stimuli, are compared and discussed across these Cell types.

  • A comparative review of Cell Culture systems for the study of Microglial biology in Alzheimer’s disease
    Journal of Neuroinflammation, 2012
    Co-Authors: Branden Stansley, J A Post, Kenneth Hensley
    Abstract:

    Over the past two decades, it has become increasingly apparent that Alzheimer’s disease neuropathology is characterized by activated microglia (brain resident macrophages) as well as the classic features of amyloid plaques and neurofibrillary tangles. The intricacy of Microglial biology has also become apparent, leading to a heightened research interest in this particular Cell type. Over the years a number of different Microglial Cell culturing techniques have been developed to study either primary mammalian microglia, or immortalized Cell lines. Each Microglial system has advantages and disadvantages and should be selected for its appropriateness in a particular research context. This review summarizes several of the most common Microglial Cell Culture systems currently being employed in Alzheimer’s research including primary microglia; BV2 and N9 retroviral immortalized microglia; human immortalized microglia (HMO6); and spontaneously immortalized rodent Microglial lines (EOC lines and HAPI Cells). Particularities of Cell Culture requirements and characteristics of Microglial behavior, especially in response to applied inflammogen stimuli, are compared and discussed across these Cell types.

J A Post - One of the best experts on this subject based on the ideXlab platform.

  • a comparative review of Cell Culture systems for the study of Microglial biology in alzheimer s disease
    Journal of Neuroinflammation, 2012
    Co-Authors: Branden Stansley, J A Post, Kenneth Hensley
    Abstract:

    Over the past two decades, it has become increasingly apparent that Alzheimer’s disease neuropathology is characterized by activated microglia (brain resident macrophages) as well as the classic features of amyloid plaques and neurofibrillary tangles. The intricacy of Microglial biology has also become apparent, leading to a heightened research interest in this particular Cell type. Over the years a number of different Microglial Cell culturing techniques have been developed to study either primary mammalian microglia, or immortalized Cell lines. Each Microglial system has advantages and disadvantages and should be selected for its appropriateness in a particular research context. This review summarizes several of the most common Microglial Cell Culture systems currently being employed in Alzheimer’s research including primary microglia; BV2 and N9 retroviral immortalized microglia; human immortalized microglia (HMO6); and spontaneously immortalized rodent Microglial lines (EOC lines and HAPI Cells). Particularities of Cell Culture requirements and characteristics of Microglial behavior, especially in response to applied inflammogen stimuli, are compared and discussed across these Cell types.

  • A comparative review of Cell Culture systems for the study of Microglial biology in Alzheimer’s disease
    Journal of Neuroinflammation, 2012
    Co-Authors: Branden Stansley, J A Post, Kenneth Hensley
    Abstract:

    Over the past two decades, it has become increasingly apparent that Alzheimer’s disease neuropathology is characterized by activated microglia (brain resident macrophages) as well as the classic features of amyloid plaques and neurofibrillary tangles. The intricacy of Microglial biology has also become apparent, leading to a heightened research interest in this particular Cell type. Over the years a number of different Microglial Cell culturing techniques have been developed to study either primary mammalian microglia, or immortalized Cell lines. Each Microglial system has advantages and disadvantages and should be selected for its appropriateness in a particular research context. This review summarizes several of the most common Microglial Cell Culture systems currently being employed in Alzheimer’s research including primary microglia; BV2 and N9 retroviral immortalized microglia; human immortalized microglia (HMO6); and spontaneously immortalized rodent Microglial lines (EOC lines and HAPI Cells). Particularities of Cell Culture requirements and characteristics of Microglial behavior, especially in response to applied inflammogen stimuli, are compared and discussed across these Cell types.

Shyam S Chaurasia - One of the best experts on this subject based on the ideXlab platform.

  • characterization of a functionally active primary Microglial Cell Culture from the pig retina
    Experimental Eye Research, 2019
    Co-Authors: Dean P Hainsworth, Rajiv R Mohan, Shyam S Chaurasia
    Abstract:

    Abstract Retinal inflammation is an integral component of many retinal diseases including diabetic retinopathy (DR), age-related macular degeneration (AMD) and retinopathy of prematurity (ROP). Inflammation is commonly initiated and perpetuated by myeloid-derived immune Cells. In the retina, Microglial Cells are resident macrophages with myeloid origins, which acts as the first responders involved in the innate immune system. To understand the disease pathogenesis, the use of isolated retinal Cell Culture model is vital for the examination of multiple Cellular responses to injury or trauma. The pig retina resembles human retina in terms of tissue architecture, vasculature, and topography. Additionally, it is a better model than the rodent retina because of the presence of the pseudomacula. In the present study, we sought to establish and characterize pig retinal primary Microglial Cell (pMicroglia) Culture. We used pig eyes from the local abattoir and optimized pMicroglia Cultures using multiple Cell Culture conditions and methods. The best results were obtained by seeding Cells in DMEM-high glucose media for 18 days followed by shaking of the Culture plate. The resulting pMicroglia were characterized by Cellular morphology, phenotype, and immunostaining with Iba-1, CD68, P2Y12, CD163, CD14, and Isolectin GS-IB4. Generated pMicroglia were found functionally active in phagocytosis assay and responsive to lipopolysaccharides (LPS) in dose-dependent production of IL-1β. Furthermore, they showed increased secretion of pro-inflammatory cytokines with LPS treatment. Thus, we report a novel and reproducible method for the isolation of primary Microglial Cells from pig eyes, which may be useful for studying retinal diseases.

Dean P Hainsworth - One of the best experts on this subject based on the ideXlab platform.

  • characterization of a functionally active primary Microglial Cell Culture from the pig retina
    Experimental Eye Research, 2019
    Co-Authors: Dean P Hainsworth, Rajiv R Mohan, Shyam S Chaurasia
    Abstract:

    Abstract Retinal inflammation is an integral component of many retinal diseases including diabetic retinopathy (DR), age-related macular degeneration (AMD) and retinopathy of prematurity (ROP). Inflammation is commonly initiated and perpetuated by myeloid-derived immune Cells. In the retina, Microglial Cells are resident macrophages with myeloid origins, which acts as the first responders involved in the innate immune system. To understand the disease pathogenesis, the use of isolated retinal Cell Culture model is vital for the examination of multiple Cellular responses to injury or trauma. The pig retina resembles human retina in terms of tissue architecture, vasculature, and topography. Additionally, it is a better model than the rodent retina because of the presence of the pseudomacula. In the present study, we sought to establish and characterize pig retinal primary Microglial Cell (pMicroglia) Culture. We used pig eyes from the local abattoir and optimized pMicroglia Cultures using multiple Cell Culture conditions and methods. The best results were obtained by seeding Cells in DMEM-high glucose media for 18 days followed by shaking of the Culture plate. The resulting pMicroglia were characterized by Cellular morphology, phenotype, and immunostaining with Iba-1, CD68, P2Y12, CD163, CD14, and Isolectin GS-IB4. Generated pMicroglia were found functionally active in phagocytosis assay and responsive to lipopolysaccharides (LPS) in dose-dependent production of IL-1β. Furthermore, they showed increased secretion of pro-inflammatory cytokines with LPS treatment. Thus, we report a novel and reproducible method for the isolation of primary Microglial Cells from pig eyes, which may be useful for studying retinal diseases.