The Experts below are selected from a list of 64764 Experts worldwide ranked by ideXlab platform
Chantal M. Boulanger - One of the best experts on this subject based on the ideXlab platform.
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In Vivo Shear Stress Determines Circulating Levels of Endothelial Microparticles in End-Stage Renal Disease
Hypertension (Dallas Tex. : 1979), 2007Co-Authors: Chantal M. Boulanger, Alain Tedgui, Nicolas Amabile, Alain P. Guerin, Bruno Pannier, Aurélie S. Leroyer, Ziad Mallat, Clément Nguyen, Gérard M. LondonAbstract:Shear stress is a major determinant of endothelial apoptosis, but its role in the in vivo release of shed membrane Microparticles by endothelial cells remains unknown. Thus, we sought to evaluate the possible relationship between circulating endothelial microparticle levels and laminar shear stress in end-stage renal disease patients with high cardiovascular risk, whose levels of endothelial Microparticles are elevated. In 34 hemodialyzed patients, we analyzed the relationships between brachial artery and aortic shear stress and circulating Microparticles levels. Only endothelial Microparticles were inversely correlated with laminar shear stress values (P
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Circulating Endothelial Microparticles Are Associated with Vascular Dysfunction in Patients with End-Stage Renal Failure
Journal of the American Society of Nephrology : JASN, 2005Co-Authors: Nicolas Amabile, Alain Tedgui, Alain P. Guerin, Aurélie S. Leroyer, Ziad Mallat, Clément Nguyen, Gérard M. London, Jacques Boddaert, Chantal M. BoulangerAbstract:Endothelial dysfunction and arterial stiffness are major determinants of cardiovascular risk in patients with end-stage renal failure (ESRF). Microparticles are membrane fragments shed from damaged or activated cells. Because Microparticles can affect endothelial cells, this study investigated the relationship between circulating Microparticles and arterial dysfunction in patients with ESRF and identified the cellular origin of Microparticles associated with these alterations. Flow cytometry analysis of platelet-free plasma from 44 patients with ESRF indicated that circulating levels of Annexin V Microparticles were increased compared with 32 healthy subjects, as were levels of Microparticles derived from endothelial cells (three-fold), platelets (16.5-fold), and erythrocytes (1.6-fold). However, when arterial function was evaluated noninvasively in patients with ESRF, only endothelial microparticle levels correlated highly with loss of flow-mediated dilation (r 0.543; P 0.004), increased aortic pulse wave velocity (r 0.642, P < 0.0001), and increased common carotid artery augmentation index (r 0.463, P 0.0017), whereas platelet-derived, erythrocyte-derived, and Annexin V microparticle levels did not. In vitro, Microparticles from patients with ESRF impaired endothelium-dependent relaxations and cyclic guanosine monophosphate generation, whereas Microparticles from healthy subjects did not. Moreover, in vitro endothelial dysfunction correlated with endothelial-derived (r 0.891; P 0.003) but not platelet-derived microparticle concentrations. In fact, endothelial Microparticles alone decreased endothelial nitric oxide release by 59 7% (P 0.025). This study suggests that circulating Microparticles of endothelial origin are tightly associated with endothelial dysfunction and arterial dysfunction in ESRF.
Min-ah Woo - One of the best experts on this subject based on the ideXlab platform.
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Facile preparation of gold-coated polydimethylsiloxane particles by in situ reduction without pre-synthesized seed A Physicochemical and engineering aspects
Colloids and Surfaces, 2017Co-Authors: Min-cheol Lim, Kisang Park, Sae-hyung Kim, Sung-wook Choi, Min-ah WooAbstract:Herein, we develop a facile strategy for the preparation of polydimethylsiloxane (PDMS) Microparticles decorated with gold nanoparticles (AuNPs) by the reduction of gold chloride on the surface of PDMS Microparticles using sodium citrate. To prepare the AuNP-seeded particles, AuNP-seeded PDMS Microparticles were simply produced in situ by the reduction of a PDMS curing agent without the use of any additional reducing agents. The resultant AuNP-seeded PDMS Microparticles were further reacted with sodium citrate and gold chloride to synthesize AuNP clusters on the PDMS microparticle surfaces. The synthesized AuNP-coated PDMS Microparticles were extensively characterized using scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and UV–vis absorption spectroscopy. Notably, the surface coverage of AuNPs on the PDMS Microparticles could be easily controlled using this proposed method.
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Facile preparation of gold-coated polydimethylsiloxane particles by in situ reduction without pre-synthesized seed
Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2017Co-Authors: Min-cheol Lim, Kisang Park, Sae-hyung Kim, Sung-wook Choi, Min-ah WooAbstract:Abstract Herein, we develop a facile strategy for the preparation of polydimethylsiloxane (PDMS) Microparticles decorated with gold nanoparticles (AuNPs) by the reduction of gold chloride on the surface of PDMS Microparticles using sodium citrate. To prepare the AuNP-seeded particles, AuNP-seeded PDMS Microparticles were simply produced in situ by the reduction of a PDMS curing agent without the use of any additional reducing agents. The resultant AuNP-seeded PDMS Microparticles were further reacted with sodium citrate and gold chloride to synthesize AuNP clusters on the PDMS microparticle surfaces. The synthesized AuNP-coated PDMS Microparticles were extensively characterized using scanning electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and UV–vis absorption spectroscopy. Notably, the surface coverage of AuNPs on the PDMS Microparticles could be easily controlled using this proposed method.
Gérard M. London - One of the best experts on this subject based on the ideXlab platform.
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In Vivo Shear Stress Determines Circulating Levels of Endothelial Microparticles in End-Stage Renal Disease
Hypertension (Dallas Tex. : 1979), 2007Co-Authors: Chantal M. Boulanger, Alain Tedgui, Nicolas Amabile, Alain P. Guerin, Bruno Pannier, Aurélie S. Leroyer, Ziad Mallat, Clément Nguyen, Gérard M. LondonAbstract:Shear stress is a major determinant of endothelial apoptosis, but its role in the in vivo release of shed membrane Microparticles by endothelial cells remains unknown. Thus, we sought to evaluate the possible relationship between circulating endothelial microparticle levels and laminar shear stress in end-stage renal disease patients with high cardiovascular risk, whose levels of endothelial Microparticles are elevated. In 34 hemodialyzed patients, we analyzed the relationships between brachial artery and aortic shear stress and circulating Microparticles levels. Only endothelial Microparticles were inversely correlated with laminar shear stress values (P
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Circulating Endothelial Microparticles Are Associated with Vascular Dysfunction in Patients with End-Stage Renal Failure
Journal of the American Society of Nephrology : JASN, 2005Co-Authors: Nicolas Amabile, Alain Tedgui, Alain P. Guerin, Aurélie S. Leroyer, Ziad Mallat, Clément Nguyen, Gérard M. London, Jacques Boddaert, Chantal M. BoulangerAbstract:Endothelial dysfunction and arterial stiffness are major determinants of cardiovascular risk in patients with end-stage renal failure (ESRF). Microparticles are membrane fragments shed from damaged or activated cells. Because Microparticles can affect endothelial cells, this study investigated the relationship between circulating Microparticles and arterial dysfunction in patients with ESRF and identified the cellular origin of Microparticles associated with these alterations. Flow cytometry analysis of platelet-free plasma from 44 patients with ESRF indicated that circulating levels of Annexin V Microparticles were increased compared with 32 healthy subjects, as were levels of Microparticles derived from endothelial cells (three-fold), platelets (16.5-fold), and erythrocytes (1.6-fold). However, when arterial function was evaluated noninvasively in patients with ESRF, only endothelial microparticle levels correlated highly with loss of flow-mediated dilation (r 0.543; P 0.004), increased aortic pulse wave velocity (r 0.642, P < 0.0001), and increased common carotid artery augmentation index (r 0.463, P 0.0017), whereas platelet-derived, erythrocyte-derived, and Annexin V microparticle levels did not. In vitro, Microparticles from patients with ESRF impaired endothelium-dependent relaxations and cyclic guanosine monophosphate generation, whereas Microparticles from healthy subjects did not. Moreover, in vitro endothelial dysfunction correlated with endothelial-derived (r 0.891; P 0.003) but not platelet-derived microparticle concentrations. In fact, endothelial Microparticles alone decreased endothelial nitric oxide release by 59 7% (P 0.025). This study suggests that circulating Microparticles of endothelial origin are tightly associated with endothelial dysfunction and arterial dysfunction in ESRF.
Nicolas Amabile - One of the best experts on this subject based on the ideXlab platform.
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In Vivo Shear Stress Determines Circulating Levels of Endothelial Microparticles in End-Stage Renal Disease
Hypertension (Dallas Tex. : 1979), 2007Co-Authors: Chantal M. Boulanger, Alain Tedgui, Nicolas Amabile, Alain P. Guerin, Bruno Pannier, Aurélie S. Leroyer, Ziad Mallat, Clément Nguyen, Gérard M. LondonAbstract:Shear stress is a major determinant of endothelial apoptosis, but its role in the in vivo release of shed membrane Microparticles by endothelial cells remains unknown. Thus, we sought to evaluate the possible relationship between circulating endothelial microparticle levels and laminar shear stress in end-stage renal disease patients with high cardiovascular risk, whose levels of endothelial Microparticles are elevated. In 34 hemodialyzed patients, we analyzed the relationships between brachial artery and aortic shear stress and circulating Microparticles levels. Only endothelial Microparticles were inversely correlated with laminar shear stress values (P
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Circulating Endothelial Microparticles Are Associated with Vascular Dysfunction in Patients with End-Stage Renal Failure
Journal of the American Society of Nephrology : JASN, 2005Co-Authors: Nicolas Amabile, Alain Tedgui, Alain P. Guerin, Aurélie S. Leroyer, Ziad Mallat, Clément Nguyen, Gérard M. London, Jacques Boddaert, Chantal M. BoulangerAbstract:Endothelial dysfunction and arterial stiffness are major determinants of cardiovascular risk in patients with end-stage renal failure (ESRF). Microparticles are membrane fragments shed from damaged or activated cells. Because Microparticles can affect endothelial cells, this study investigated the relationship between circulating Microparticles and arterial dysfunction in patients with ESRF and identified the cellular origin of Microparticles associated with these alterations. Flow cytometry analysis of platelet-free plasma from 44 patients with ESRF indicated that circulating levels of Annexin V Microparticles were increased compared with 32 healthy subjects, as were levels of Microparticles derived from endothelial cells (three-fold), platelets (16.5-fold), and erythrocytes (1.6-fold). However, when arterial function was evaluated noninvasively in patients with ESRF, only endothelial microparticle levels correlated highly with loss of flow-mediated dilation (r 0.543; P 0.004), increased aortic pulse wave velocity (r 0.642, P < 0.0001), and increased common carotid artery augmentation index (r 0.463, P 0.0017), whereas platelet-derived, erythrocyte-derived, and Annexin V microparticle levels did not. In vitro, Microparticles from patients with ESRF impaired endothelium-dependent relaxations and cyclic guanosine monophosphate generation, whereas Microparticles from healthy subjects did not. Moreover, in vitro endothelial dysfunction correlated with endothelial-derived (r 0.891; P 0.003) but not platelet-derived microparticle concentrations. In fact, endothelial Microparticles alone decreased endothelial nitric oxide release by 59 7% (P 0.025). This study suggests that circulating Microparticles of endothelial origin are tightly associated with endothelial dysfunction and arterial dysfunction in ESRF.
Aurélie S. Leroyer - One of the best experts on this subject based on the ideXlab platform.
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In Vivo Shear Stress Determines Circulating Levels of Endothelial Microparticles in End-Stage Renal Disease
Hypertension (Dallas Tex. : 1979), 2007Co-Authors: Chantal M. Boulanger, Alain Tedgui, Nicolas Amabile, Alain P. Guerin, Bruno Pannier, Aurélie S. Leroyer, Ziad Mallat, Clément Nguyen, Gérard M. LondonAbstract:Shear stress is a major determinant of endothelial apoptosis, but its role in the in vivo release of shed membrane Microparticles by endothelial cells remains unknown. Thus, we sought to evaluate the possible relationship between circulating endothelial microparticle levels and laminar shear stress in end-stage renal disease patients with high cardiovascular risk, whose levels of endothelial Microparticles are elevated. In 34 hemodialyzed patients, we analyzed the relationships between brachial artery and aortic shear stress and circulating Microparticles levels. Only endothelial Microparticles were inversely correlated with laminar shear stress values (P
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Circulating Endothelial Microparticles Are Associated with Vascular Dysfunction in Patients with End-Stage Renal Failure
Journal of the American Society of Nephrology : JASN, 2005Co-Authors: Nicolas Amabile, Alain Tedgui, Alain P. Guerin, Aurélie S. Leroyer, Ziad Mallat, Clément Nguyen, Gérard M. London, Jacques Boddaert, Chantal M. BoulangerAbstract:Endothelial dysfunction and arterial stiffness are major determinants of cardiovascular risk in patients with end-stage renal failure (ESRF). Microparticles are membrane fragments shed from damaged or activated cells. Because Microparticles can affect endothelial cells, this study investigated the relationship between circulating Microparticles and arterial dysfunction in patients with ESRF and identified the cellular origin of Microparticles associated with these alterations. Flow cytometry analysis of platelet-free plasma from 44 patients with ESRF indicated that circulating levels of Annexin V Microparticles were increased compared with 32 healthy subjects, as were levels of Microparticles derived from endothelial cells (three-fold), platelets (16.5-fold), and erythrocytes (1.6-fold). However, when arterial function was evaluated noninvasively in patients with ESRF, only endothelial microparticle levels correlated highly with loss of flow-mediated dilation (r 0.543; P 0.004), increased aortic pulse wave velocity (r 0.642, P < 0.0001), and increased common carotid artery augmentation index (r 0.463, P 0.0017), whereas platelet-derived, erythrocyte-derived, and Annexin V microparticle levels did not. In vitro, Microparticles from patients with ESRF impaired endothelium-dependent relaxations and cyclic guanosine monophosphate generation, whereas Microparticles from healthy subjects did not. Moreover, in vitro endothelial dysfunction correlated with endothelial-derived (r 0.891; P 0.003) but not platelet-derived microparticle concentrations. In fact, endothelial Microparticles alone decreased endothelial nitric oxide release by 59 7% (P 0.025). This study suggests that circulating Microparticles of endothelial origin are tightly associated with endothelial dysfunction and arterial dysfunction in ESRF.
