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Travis C Glenn - One of the best experts on this subject based on the ideXlab platform.

  • significant variance in genetic diversity among populations of schistosoma haematobium detected using Microsatellite DNA loci from a genome wide database
    Parasites & Vectors, 2013
    Co-Authors: Travis C Glenn, Stacey L Lance, Anna M Mckee, Bonnie L Webster, Aidan M Emery, Adhemar Zerlotini, Guilherme Oliveira, David Rollinson
    Abstract:

    Background Urogenital schistosomiasis caused by Schistosoma haematobium is widely distributed across Africa and is increasingly being targeted for control. Genome sequences and population genetic parameters can give insight into the potential for population- or species-level drug resistance. Microsatellite DNA loci are genetic markers in wide use by Schistosoma researchers, but there are few primers available for S. haematobium.

  • isolation and characterization of 14 polymorphic Microsatellite DNA loci for the endangered whooping crane grus americana and their applicability to other crane species
    Conservation Genetics Resources, 2010
    Co-Authors: Kenneth L. Jones, Jessica R Henkel, Jerome J Howard, Stacey L Lance, Chris Hagen, Travis C Glenn
    Abstract:

    Fourteen Microsatellite DNA loci were isolated from the endangered Whooping Crane (Grus americana) and genetic variability assessed from 45 captive reared individuals. Allele numbers detected at each locus ranged from 2 to 6, the highest seen for this species. Mean observed heterozygosity varied from 0.04 to 0.79. These markers were then successfully amplified for two non-migratory populations of Sandhill Crane [Florida (Grus canadensis pratensis) and Missisippi (Grus canadensis pulla)], underscoring their utility for the conservation of threatened crane species.

  • isolating Microsatellite DNA loci
    Methods in Enzymology, 2005
    Co-Authors: Travis C Glenn, Nancy A Schable
    Abstract:

    A series of techniques are presented to construct genomic DNA libraries highly enriched for Microsatellite DNA loci. The individual techniques used here derive from several published protocols but have been optimized and tested in our research laboratories as well as in classroom settings at the University of South Carolina and University of Georgia, with students achieving nearly 100% success. Reducing the number of manipulations involved has been a key to success, decreasing both the failure rate and the time necessary to isolate loci of interest. In our lab during the past 3 years alone, these protocols have been successfully used to isolate Microsatellite DNA loci from at least 55 species representing three kingdoms. These protocols have made it possible to reduce the time to identify candidate loci for primer development from most eukaryotic species to as little as 1 week.

  • characterization of Microsatellite DNA loci for the southern flying squirrel glaucomys volans
    Molecular Ecology Notes, 2003
    Co-Authors: Bobby H Fokidis, Cris Hagen, Nancy A Schable, Travis C Glenn, Thomas S Risch
    Abstract:

    Polymerase chain reaction primers for Microsatellite DNA loci (one dinucleotide, four tetranucleotide and two compound) and the conditions necessary to amplify each are described for the southern flying squirrel ( Glaucomys volans ). These primers were tested on 22 or more individuals from a population at the Savannah River Site in South Carolina. These Microsatellite primers yielded a high allelic diversity (6–22 alleles/locus), and moderate to high observed heterozygosities (0.318–0.826). Primers developed for the northern flying squirrel ( Glaucomys sabrinus ) were also tested for use on G. volans , with only two successful cross amplifications from the seven loci.

  • seven polymorphic Microsatellite DNA loci from the red spotted newt notophthalmus viridescens
    Molecular Ecology Notes, 2003
    Co-Authors: Dean A Croshaw, Travis C Glenn
    Abstract:

    We describe polymerase chain reaction (PCR) primers and amplification conditions for seven Microsatellite DNA loci isolated from the red-spotted newt (Notophthalmus viridescens). Primers were tested on 16 individuals from two populations on the Savannah River Site in Aiken County, South Carolina. We detected six to 10 alleles per locus and an overall observed heterozygosity range of 0.31–0.81. Despite low heterozygosity at two of the seven loci, the high polymorphic information contents (from 0.54 to 0.85) of these markers render them useful for future studies of the behavioural and population ecology of this common salamander.

Nobuhiko Taniguchi - One of the best experts on this subject based on the ideXlab platform.

  • genetic evaluation of interindividual relatedness for broodstock management of the rare species barfin flounder verasper moseri using Microsatellite DNA markers
    Fisheries Science, 2006
    Co-Authors: Maria Del Mar Ortegavillaizan Romo, Masamichi Nakajima, Shigenori Suzuki, Nobuhiko Taniguchi
    Abstract:

    The barfin flounder has been recently declared to be rare, as the number of wild individuals in Japan has greatly reduced since 1975. The Japanese National Center for Stock Enhancement started the stocking program for this species on 1987. In the present study, Microsatellite DNA loci were applied in order to determine the pedigree of the hatchery-produced juveniles for stock enhancement. Additionally, using six polymorphic Microsatellite markers, the accuracy to assign dyads to categories of kinship was estimated using the relatedness estimator (R xy ), similarity indexes (individual genetic identity [GI] and proportion of shared alleles [P s ], identification of kin groups using unweighted pair group method with arithmetic mean (UPGMA) clustering based on interindividual genetic distances (1-R xy, 1-P s, and 1-Gl), a likelihood ratio approach, and partition methods to separate individuals into cohorts. The results were compared with the pedigree relationship previously obtained from parentage analysis. Estimation of the kinship relationship between individuals could be better inferred by means of UPGMA dendrogram clustering based on 1-Gl or 1-P s as interindividual genetic distances, or using computer software to perform partitioning of set of individuals.

  • linkage mapping of aflp and Microsatellite DNA markers with the body color and sex determining loci in the guppy poecilia reticulata
    Zoological Science, 2005
    Co-Authors: Tomohisa Watanabe, Minoru Yoshida, Masamichi Nakajima, Nobuhiko Taniguchi
    Abstract:

    The guppy is an ornamental fish species that exhibits various phenotypic characteristics, such as body color and fin-shape. Although linkage relationships of a limited number of phenotypic traits have already been investigated, the association between phenotypic and molecular markers is still unknown. We constructed a total of 35 linkage groups for the guppy using 186 polymorphic loci of AFLP and Microsatellite DNA. The locus related to the yellow body color was linked with ten markers and the sex-determination locus was linked with five markers.

  • genetic variability and pedigree tracing of a hatchery reared stock of red sea bream pagrus major used for stock enhancement based on Microsatellite DNA markers
    Aquaculture, 1999
    Co-Authors: Ricardo Perezenriquez, Motohiro Takagi, Nobuhiko Taniguchi
    Abstract:

    Abstract Stock enhancement programs that use a small number of breeders for the production of hatchery-reared juveniles to be released to the environment, may have negative effects on the genetic diversity of wild populations due to a reduced genetic variability of the released stock. This study compared the genetic diversity of a hatchery-reared stock of red sea bream ( Pagrus major ) used for stock enhancement with that of their broodstock. Its pedigree was also traced, using four to five Microsatellite DNA markers, to quantify the actual number of reproducing parents. Then, the effective number of contributing parents ( N e ) and the inbreeding coefficient were estimated. It was found that the genetic diversity of the hatchery-reared stock in terms of the mean observed heterozygosity ( H o =0.856), was not significant different ( P >0.05) than that of the broodstock ( H o =0.841). However, significant differences ( P N e was N e =63.7, consequently the estimated inbreeding coefficient was less than 0.8%. The results provide no evidence to consider a loss of genetic variation of the hatchery-reared stock, and a discussion on the possible effects of its release is presented.

  • genetic variability and pedigree tracing of a hatchery reared stock of red sea bream pagrus major used for stock enhancement based on Microsatellite DNA markers
    Aquaculture, 1999
    Co-Authors: Ricardo Perezenriquez, Motohiro Takagi, Nobuhiko Taniguchi
    Abstract:

    Abstract Stock enhancement programs that use a small number of breeders for the production of hatchery-reared juveniles to be released to the environment, may have negative effects on the genetic diversity of wild populations due to a reduced genetic variability of the released stock. This study compared the genetic diversity of a hatchery-reared stock of red sea bream (Pagrus major) used for stock enhancement with that of their broodstock. Its pedigree was also traced, using four to five Microsatellite DNA markers, to quantify the actual number of reproducing parents. Then, the effective number of contributing parents (Ne) and the inbreeding coefficient were estimated. It was found that the genetic diversity of the hatchery-reared stock in terms of the mean observed heterozygosity (Ho=0.856), was not significant different (P>0.05) than that of the broodstock (Ho=0.841). However, significant differences (P

Ricardo Perezenriquez - One of the best experts on this subject based on the ideXlab platform.

  • genetic variability and pedigree tracing of a hatchery reared stock of red sea bream pagrus major used for stock enhancement based on Microsatellite DNA markers
    Aquaculture, 1999
    Co-Authors: Ricardo Perezenriquez, Motohiro Takagi, Nobuhiko Taniguchi
    Abstract:

    Abstract Stock enhancement programs that use a small number of breeders for the production of hatchery-reared juveniles to be released to the environment, may have negative effects on the genetic diversity of wild populations due to a reduced genetic variability of the released stock. This study compared the genetic diversity of a hatchery-reared stock of red sea bream ( Pagrus major ) used for stock enhancement with that of their broodstock. Its pedigree was also traced, using four to five Microsatellite DNA markers, to quantify the actual number of reproducing parents. Then, the effective number of contributing parents ( N e ) and the inbreeding coefficient were estimated. It was found that the genetic diversity of the hatchery-reared stock in terms of the mean observed heterozygosity ( H o =0.856), was not significant different ( P >0.05) than that of the broodstock ( H o =0.841). However, significant differences ( P N e was N e =63.7, consequently the estimated inbreeding coefficient was less than 0.8%. The results provide no evidence to consider a loss of genetic variation of the hatchery-reared stock, and a discussion on the possible effects of its release is presented.

  • genetic variability and pedigree tracing of a hatchery reared stock of red sea bream pagrus major used for stock enhancement based on Microsatellite DNA markers
    Aquaculture, 1999
    Co-Authors: Ricardo Perezenriquez, Motohiro Takagi, Nobuhiko Taniguchi
    Abstract:

    Abstract Stock enhancement programs that use a small number of breeders for the production of hatchery-reared juveniles to be released to the environment, may have negative effects on the genetic diversity of wild populations due to a reduced genetic variability of the released stock. This study compared the genetic diversity of a hatchery-reared stock of red sea bream (Pagrus major) used for stock enhancement with that of their broodstock. Its pedigree was also traced, using four to five Microsatellite DNA markers, to quantify the actual number of reproducing parents. Then, the effective number of contributing parents (Ne) and the inbreeding coefficient were estimated. It was found that the genetic diversity of the hatchery-reared stock in terms of the mean observed heterozygosity (Ho=0.856), was not significant different (P>0.05) than that of the broodstock (Ho=0.841). However, significant differences (P

Dennis Hedgecock - One of the best experts on this subject based on the ideXlab platform.

  • Linkage maps of Microsatellite DNA markers for the pacific oyster Crassostrea gigas
    Genetics, 2004
    Co-Authors: Sophie Hubert, Dennis Hedgecock
    Abstract:

    We constructed male and female consensus linkage maps for the Pacific oyster Crassostrea gigas, using a total of 102 Microsatellite DNA markers typed in 11-day-old larvae from three families. We identified 11 and 12 linkage groups in the male and female consensus maps, respectively. Alignment of these separate maps, however, suggests 10 linkage groups, which agrees with the haploid chromosome number. The male linkage map comprises 88 loci and spans 616.1 cM, while the female map comprises 86 loci and spans 770.5 cM. The male and the female maps share 74 loci; 2 markers remain unlinked. The estimated coverages for the consensus linkage maps are 79% for the male and 70-75% for the female, on the basis of two estimates of genome length. Ninety-five percent of the genome is expected to lie within 16 and 21 cM of markers on the male and female maps, respectively, while 95% of simulated minimum distances to the male and female maps are within 10.1 and 13.6 cM, respectively. Females have significantly more recombination than males, across 118 pairs of linked markers in common to the parents of the three families. Significant differences in recombination and orders of markers are also evident among same-sex parents of different families as well as sibling parents of opposite sex. These observations suggest that polymorphism for chromosomal rearrangements may exist in natural populations, which could have profound implications for interpreting the evolutionary genetics of the oyster. These are the first linkage maps for a bivalve mollusc that use Microsatellite DNA markers, which should enable them to be transferred to other families and to be useful for further genetic analyses such as QTL mapping.

  • characterization of 79 Microsatellite DNA markers in the pacific oyster crassostrea gigas
    Molecular Ecology Notes, 2003
    Co-Authors: Sophie Hubert, Katherine Bucklin, Vanessa Ribes, Dennis Hedgecock
    Abstract:

    We characterized 79 Microsatellite DNA markers, which were obtained from genomic libraries enriched for CA, GA, ATG and TAGA motif repeats, in the Pacific oyster Crassostrea gigas. For eight F1 grandparents or great-grandparents of mapping families, the average heterozygosity, 0.705, and average number of alleles per locus, 5.7, did not vary among motif-repeat or motif-complexity categories. Non-amplifying polymerase chain reaction null alleles, which were confirmed by segregation in the mapping families, were detected at 41 (51.9%) of the 79 loci. Cross-species amplifications from C. angulata, C. sikamea, C. ariakensis and C. virginica showed a precipitous decline with distance from the focal species C. gigas.

Motohiro Takagi - One of the best experts on this subject based on the ideXlab platform.

  • genetic variability and pedigree tracing of a hatchery reared stock of red sea bream pagrus major used for stock enhancement based on Microsatellite DNA markers
    Aquaculture, 1999
    Co-Authors: Ricardo Perezenriquez, Motohiro Takagi, Nobuhiko Taniguchi
    Abstract:

    Abstract Stock enhancement programs that use a small number of breeders for the production of hatchery-reared juveniles to be released to the environment, may have negative effects on the genetic diversity of wild populations due to a reduced genetic variability of the released stock. This study compared the genetic diversity of a hatchery-reared stock of red sea bream ( Pagrus major ) used for stock enhancement with that of their broodstock. Its pedigree was also traced, using four to five Microsatellite DNA markers, to quantify the actual number of reproducing parents. Then, the effective number of contributing parents ( N e ) and the inbreeding coefficient were estimated. It was found that the genetic diversity of the hatchery-reared stock in terms of the mean observed heterozygosity ( H o =0.856), was not significant different ( P >0.05) than that of the broodstock ( H o =0.841). However, significant differences ( P N e was N e =63.7, consequently the estimated inbreeding coefficient was less than 0.8%. The results provide no evidence to consider a loss of genetic variation of the hatchery-reared stock, and a discussion on the possible effects of its release is presented.

  • genetic variability and pedigree tracing of a hatchery reared stock of red sea bream pagrus major used for stock enhancement based on Microsatellite DNA markers
    Aquaculture, 1999
    Co-Authors: Ricardo Perezenriquez, Motohiro Takagi, Nobuhiko Taniguchi
    Abstract:

    Abstract Stock enhancement programs that use a small number of breeders for the production of hatchery-reared juveniles to be released to the environment, may have negative effects on the genetic diversity of wild populations due to a reduced genetic variability of the released stock. This study compared the genetic diversity of a hatchery-reared stock of red sea bream (Pagrus major) used for stock enhancement with that of their broodstock. Its pedigree was also traced, using four to five Microsatellite DNA markers, to quantify the actual number of reproducing parents. Then, the effective number of contributing parents (Ne) and the inbreeding coefficient were estimated. It was found that the genetic diversity of the hatchery-reared stock in terms of the mean observed heterozygosity (Ho=0.856), was not significant different (P>0.05) than that of the broodstock (Ho=0.841). However, significant differences (P