The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Craig Bakeraustin - One of the best experts on this subject based on the ideXlab platform.
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high prevalence of multidrug tolerant bacteria and associated antimicrobial resistance genes isolated from Ornamental Fish and their carriage water
PLOS ONE, 2009Co-Authors: David W Vernerjeffreys, Michelle J Pond, Martin J Woodward, Georgina S E Rimmer, Victoria Morrison, Tamar Schwarz, Timothy J. Welch, E. Roberts, Sarah-jane Haig, Craig BakeraustinAbstract:Background Antimicrobials are used to directly control bacterial infections in pet (Ornamental) Fish and are routinely added to the water these Fish are shipped in to suppress the growth of potential pathogens during transport. Methodology/Principal Findings To assess the potential effects of this sustained selection pressure, 127 Aeromonas spp. isolated from warm and cold water Ornamental Fish species were screened for tolerance to 34 antimicrobials. Representative isolates were also examined for the presence of 54 resistance genes by a combination of miniaturized microarray and conventional PCR. Forty-seven of 94 Aeromonas spp. isolates recovered from tropical Ornamental Fish and their carriage water were tolerant to ≥15 antibiotics, representing seven or more different classes of antimicrobial. The quinolone and fluoroquinolone resistance gene, qnrS2, was detected at high frequency (37% tested recent isolates were positive by PCR). Class 1 integrons, IncA/C broad host range plasmids and a range of other antibiotic resistance genes, including floR, blaTEM−1, tet(A), tet(D), tet(E), qacE2, sul1, and a number of different dihydrofolate reductase and aminoglycoside transferase coding genes were also detected in carriage water samples and bacterial isolates. Conclusions These data suggest that Ornamental Fish and their carriage water act as a reservoir for both multi-resistant bacteria and resistance genes.
Neil Stockenstrom Gardiner - One of the best experts on this subject based on the ideXlab platform.
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isolation and selection of bacillus spp as potential biological agents for enhancement of water quality in culture of Ornamental Fish
Journal of Applied Microbiology, 2007Co-Authors: Rajesh Lalloo, Santosh Ramchuran, D Ramduth, Johann F Gorgens, Neil Stockenstrom GardinerAbstract:Aims: To isolate, select and evaluate Bacillus spp. as potential biological agents for enhancement of water quality in culture of Ornamental Fish. Methods and Results: Natural isolates obtained from mud sediment and Cyprinus carpio were purified and assessed in vitro for efficacy based on the inhibition of growth of pathogenic Aeromonas hydrophila and the decrease in concentrations of ammonium, nitrite, nitrate and phosphate ions. Based on suitability to predefined characteristics, the isolates B001, B002 and B003 were selected and evaluated in vitro in the presence of Aer. hydrophila and in a preliminary in vivo trial with C. carpio. The inhibitory effect on pathogen growth and the decrease in concentrations of waste ions was demonstrated. Based on 16S RNA sequence homology, the isolates were identified as Bacillus subtilis, Bacillus cereus and Bacillus licheniformis, respectively. Isolate B002 did not contain the anthrax virulence plasmids pOX1, pOX2 or the B. cereus enterotoxin. Conclusions: Selected isolates effected synergistic reduction in pathogen load and the concentrations of waste ions in vitro and in vivo and are safe for use in Ornamental aquaculture. Significance and Impact of the Study: A new approach for assessment of biological agents was demonstrated and has yielded putative isolates for development into aquaculture products.
Monika Dolejska - One of the best experts on this subject based on the ideXlab platform.
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Ornamental Fish as a source of plasmid mediated quinolone resistance genes and antibiotic resistance plasmids
Veterinary Microbiology, 2014Co-Authors: Hana Dobiasova, Iva Kutilova, Veronika Piackova, T Vesely, Alois Cizek, Monika DolejskaAbstract:Abstract Growing Ornamental Fish industry is associated with public health concerns including extensive antibiotic use accompanied by increasing antibiotic resistance. The aim of this study was to analyze Aeromonas isolates from imported tropical Ornamental Fish and coldwater koi carps bred in the Czech Republic to assess the potential risk of Ornamental Fish as a source of plasmid-mediated quinolone resistance genes (PMQR) and antibiotic resistance plasmids. A collection of Aeromonas spp. with reduced susceptibility to ciprofloxacin (MIC ≥ 0.05 mg/L) was selected for the detection of PMQR genes. Isolates harbouring PMQR genes were further analyzed for the additional antibiotic resistance, integron content, clonality, biofilm production and transferability of PMQR genes by conjugation and transformation. Comparative analysis of plasmids carrying PMQR genes was performed. Fifteen (19%, n = 80) isolates from koi carps and 18 (24%, n = 76) isolates from imported Ornamental Fish were positive for qnrS2 , aac(6′)-Ib-cr or qnrB17 genes. PMQR-positive isolates from imported Ornamental Fish showed higher MIC levels to quinolones, multiresistance and diverse content of antibiotic resistance genes and integrons compared to the isolates from the carps. Related IncU plasmids harbouring qnrS2 and aac(6′)-Ib-cr genes were found in Aeromonas spp. from imported Ornamental Fish and koi carps from various geographical areas. Ornamental Fish may represent a potential source of multiresistant bacteria and mobile genetic elements for the environment and for humans.
David W Vernerjeffreys - One of the best experts on this subject based on the ideXlab platform.
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high prevalence of multidrug tolerant bacteria and associated antimicrobial resistance genes isolated from Ornamental Fish and their carriage water
PLOS ONE, 2009Co-Authors: David W Vernerjeffreys, Michelle J Pond, Martin J Woodward, Georgina S E Rimmer, Victoria Morrison, Tamar Schwarz, Timothy J. Welch, E. Roberts, Sarah-jane Haig, Craig BakeraustinAbstract:Background Antimicrobials are used to directly control bacterial infections in pet (Ornamental) Fish and are routinely added to the water these Fish are shipped in to suppress the growth of potential pathogens during transport. Methodology/Principal Findings To assess the potential effects of this sustained selection pressure, 127 Aeromonas spp. isolated from warm and cold water Ornamental Fish species were screened for tolerance to 34 antimicrobials. Representative isolates were also examined for the presence of 54 resistance genes by a combination of miniaturized microarray and conventional PCR. Forty-seven of 94 Aeromonas spp. isolates recovered from tropical Ornamental Fish and their carriage water were tolerant to ≥15 antibiotics, representing seven or more different classes of antimicrobial. The quinolone and fluoroquinolone resistance gene, qnrS2, was detected at high frequency (37% tested recent isolates were positive by PCR). Class 1 integrons, IncA/C broad host range plasmids and a range of other antibiotic resistance genes, including floR, blaTEM−1, tet(A), tet(D), tet(E), qacE2, sul1, and a number of different dihydrofolate reductase and aminoglycoside transferase coding genes were also detected in carriage water samples and bacterial isolates. Conclusions These data suggest that Ornamental Fish and their carriage water act as a reservoir for both multi-resistant bacteria and resistance genes.
Alois Cizek - One of the best experts on this subject based on the ideXlab platform.
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characterization of microbiota composition and presence of selected antibiotic resistance genes in carriage water of Ornamental Fish
PLOS ONE, 2014Co-Authors: Lenka Gerzova, Alois Cizek, Petra Videnska, Marcela Faldynova, Karel Sedlar, Ivo Provaznik, Ivan RychlikAbstract:International trade with Ornamental Fish is gradually recognized as an important source of a wide range of different antibiotic resistant bacteria. In this study we therefore characterized the prevalence of selected antibiotic resistance genes in the microbiota found in the carriage water of Ornamental Fish originating from 3 different continents. Real-time PCR quantification showed that the sul1 gene was present in 11 out of 100 bacteria. tet(A) was present in 6 out of 100 bacteria and strA, tet(G), sul2 and aadA were present in 1–2 copies per 100 bacteria. Class I integrons were quite common in carriage water microbiota, however, pyrosequencing showed that only 12 different antibiotic gene cassettes were present in class I integrons. The microbiota characterized by pyrosequencing of the V3/V4 variable region of 16S rRNA genes consisted of Proteobacteria (48%), Bacteroidetes (29.5%), Firmicutes (17.8%), Actinobacteria (2.1%) and Fusobacteria (1.6%). Correlation analysis between antibiotic resistance gene prevalence and microbiota composition verified by bacterial culture showed that major reservoirs of sul1 sul2, tet(A), tet(B) tet(G), cat, cml, bla, strA, aacA, aph and aadA could be found among Alpha-, Beta- and Gammaproteobacteria with representatives of Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae and Comamonadaceae being those most positively associated with the tested antibiotic resistance genes.
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Ornamental Fish as a source of plasmid mediated quinolone resistance genes and antibiotic resistance plasmids
Veterinary Microbiology, 2014Co-Authors: Hana Dobiasova, Iva Kutilova, Veronika Piackova, T Vesely, Alois Cizek, Monika DolejskaAbstract:Abstract Growing Ornamental Fish industry is associated with public health concerns including extensive antibiotic use accompanied by increasing antibiotic resistance. The aim of this study was to analyze Aeromonas isolates from imported tropical Ornamental Fish and coldwater koi carps bred in the Czech Republic to assess the potential risk of Ornamental Fish as a source of plasmid-mediated quinolone resistance genes (PMQR) and antibiotic resistance plasmids. A collection of Aeromonas spp. with reduced susceptibility to ciprofloxacin (MIC ≥ 0.05 mg/L) was selected for the detection of PMQR genes. Isolates harbouring PMQR genes were further analyzed for the additional antibiotic resistance, integron content, clonality, biofilm production and transferability of PMQR genes by conjugation and transformation. Comparative analysis of plasmids carrying PMQR genes was performed. Fifteen (19%, n = 80) isolates from koi carps and 18 (24%, n = 76) isolates from imported Ornamental Fish were positive for qnrS2 , aac(6′)-Ib-cr or qnrB17 genes. PMQR-positive isolates from imported Ornamental Fish showed higher MIC levels to quinolones, multiresistance and diverse content of antibiotic resistance genes and integrons compared to the isolates from the carps. Related IncU plasmids harbouring qnrS2 and aac(6′)-Ib-cr genes were found in Aeromonas spp. from imported Ornamental Fish and koi carps from various geographical areas. Ornamental Fish may represent a potential source of multiresistant bacteria and mobile genetic elements for the environment and for humans.