The Experts below are selected from a list of 219 Experts worldwide ranked by ideXlab platform
Holger Gärtner - One of the best experts on this subject based on the ideXlab platform.
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a new sledge Microtome to combine wood anatomy and tree ring ecology
Iawa Journal, 2015Co-Authors: Holger Gärtner, S Lucchinetti, Fritz H SchweingruberAbstract:Based on the Reichert Om E Microtome a more sophisticated, nevertheless solely mechanically operated Microtome was developed enabling to section specimens of various forms and sizes. The materials used and the special construction of the Microtome resulted in a higher stability of the moving parts whilst simultaneously reducing weight. As a result of the high stability of the sledge guidance, bigger sections can be cut enabling more detailed analyses of anatomical structures within plant stems, roots or branches as well as their variation back in time. Consequently, wood anatomical parameters can be integrated more easily in time-series analyses supporting the aims of a quantitative wood ecology.
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new perspectives for wood anatomical analysis in dendrosciences the gsl1 Microtome
Dendrochronologia, 2014Co-Authors: Holger Gärtner, Sandro Lucchinetti, Fritz H SchweingruberAbstract:a b s t r a c t The variability of wood anatomical features in the rings of trees and shrubs is known to be dependent on multifaceted environmental parameters. The ability to determine anatomical variations over longer time periods as decades or centuries is a step forward in dendroecological and dendroclimatological research. In this regard micro sectioning is still one of the basic requirements but sectioning devices (Microtomes) designed for wood anatomical purposes are rarely available. We present an affordable heavy duty, but light-weight Microtome operated with removable blades. This portable device enables the production of large sections for dendroclimatological reconstructions as well as various types of common sections for wood anatomical studies. © 2013 Elsevier GmbH. All rights reserved.
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The core-Microtome: A new tool for surface preparation on cores and time series analysis of varying cell parameters
Dendrochronologia, 2010Co-Authors: Holger Gärtner, Daniel NievergeltAbstract:A Microtome designed for the surface preparation of entire increment cores allows cutting plane surfaces on cores up to a length of 40 cm. Compared to the common sanding procedure, the wood cells of the annual rings remain open, not filled with swarf, and the cell walls are smooth and hence clearly visible. This article aims at describing the functionality of the Microtome and the procedures needed for an accurate surface preparation to achieve a good contrast for subsequent image analysis. Possible applications for a more detailed analysis of variations in the tracheid structure of conifers and vessel sizes of oak are presented, which can be included in time series analyses. © 2009 Elsevier GmbH. All rights reserved.
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the core Microtome a new tool for surface preparation on cores and time series analysis of varying cell parameters
Dendrochronologia, 2010Co-Authors: Holger Gärtner, Daniel NievergeltAbstract:A Microtome designed for the surface preparation of entire increment cores allows cutting plane surfaces on cores up to a length of 40 cm. Compared to the common sanding procedure, the wood cells of the annual rings remain open, not filled with swarf, and the cell walls are smooth and hence clearly visible. This article aims at describing the functionality of the Microtome and the procedures needed for an accurate surface preparation to achieve a good contrast for subsequent image analysis. Possible applications for a more detailed analysis of variations in the tracheid structure of conifers and vessel sizes of oak are presented, which can be included in time series analyses.
Daniel Nievergelt - One of the best experts on this subject based on the ideXlab platform.
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The core-Microtome: A new tool for surface preparation on cores and time series analysis of varying cell parameters
Dendrochronologia, 2010Co-Authors: Holger Gärtner, Daniel NievergeltAbstract:A Microtome designed for the surface preparation of entire increment cores allows cutting plane surfaces on cores up to a length of 40 cm. Compared to the common sanding procedure, the wood cells of the annual rings remain open, not filled with swarf, and the cell walls are smooth and hence clearly visible. This article aims at describing the functionality of the Microtome and the procedures needed for an accurate surface preparation to achieve a good contrast for subsequent image analysis. Possible applications for a more detailed analysis of variations in the tracheid structure of conifers and vessel sizes of oak are presented, which can be included in time series analyses. © 2009 Elsevier GmbH. All rights reserved.
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the core Microtome a new tool for surface preparation on cores and time series analysis of varying cell parameters
Dendrochronologia, 2010Co-Authors: Holger Gärtner, Daniel NievergeltAbstract:A Microtome designed for the surface preparation of entire increment cores allows cutting plane surfaces on cores up to a length of 40 cm. Compared to the common sanding procedure, the wood cells of the annual rings remain open, not filled with swarf, and the cell walls are smooth and hence clearly visible. This article aims at describing the functionality of the Microtome and the procedures needed for an accurate surface preparation to achieve a good contrast for subsequent image analysis. Possible applications for a more detailed analysis of variations in the tracheid structure of conifers and vessel sizes of oak are presented, which can be included in time series analyses.
Hendrik Falck - One of the best experts on this subject based on the ideXlab platform.
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a sledge Microtome for high resolution subsampling of freeze cores
Journal of Paleolimnology, 2011Co-Authors: Andrew L Macumber, Timothy R Patterson, Lisa A Neville, Hendrik FalckAbstract:We describe a sledge Microtome designed for the high-resolution subsampling of freeze cores. This inexpensive freeze-core Microtome is capable of producing precise subsamples at mm to sub-mm resolution without sediment loss and cross-contamination. Such a subsampling resolution permits recognition of sub-decadal to annual events even in systems with low sedimentation rates. The freeze-core Microtome is particularly useful for obtaining high-resolution subsamples at the environmentally important sediment-water interface due to freeze corers being capable of capturing this boundary with minimal disturbance as compared to other coring methods.
Fritz H Schweingruber - One of the best experts on this subject based on the ideXlab platform.
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a new sledge Microtome to combine wood anatomy and tree ring ecology
Iawa Journal, 2015Co-Authors: Holger Gärtner, S Lucchinetti, Fritz H SchweingruberAbstract:Based on the Reichert Om E Microtome a more sophisticated, nevertheless solely mechanically operated Microtome was developed enabling to section specimens of various forms and sizes. The materials used and the special construction of the Microtome resulted in a higher stability of the moving parts whilst simultaneously reducing weight. As a result of the high stability of the sledge guidance, bigger sections can be cut enabling more detailed analyses of anatomical structures within plant stems, roots or branches as well as their variation back in time. Consequently, wood anatomical parameters can be integrated more easily in time-series analyses supporting the aims of a quantitative wood ecology.
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new perspectives for wood anatomical analysis in dendrosciences the gsl1 Microtome
Dendrochronologia, 2014Co-Authors: Holger Gärtner, Sandro Lucchinetti, Fritz H SchweingruberAbstract:a b s t r a c t The variability of wood anatomical features in the rings of trees and shrubs is known to be dependent on multifaceted environmental parameters. The ability to determine anatomical variations over longer time periods as decades or centuries is a step forward in dendroecological and dendroclimatological research. In this regard micro sectioning is still one of the basic requirements but sectioning devices (Microtomes) designed for wood anatomical purposes are rarely available. We present an affordable heavy duty, but light-weight Microtome operated with removable blades. This portable device enables the production of large sections for dendroclimatological reconstructions as well as various types of common sections for wood anatomical studies. © 2013 Elsevier GmbH. All rights reserved.
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Microtome Sections of Charcoal: – Technical Note –
Iawa Journal, 2012Co-Authors: Fritz H SchweingruberAbstract:This note describes and illustrates a rapid and user-friendly method to section fragile charcoals by Microtome after applying two component adhesive to the transverse charcoal surface and using adhesive tape to prevent the sections to disintegrate.
Junji Sugiyama - One of the best experts on this subject based on the ideXlab platform.
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On the detachment of the gelatinous layer in tension wood fiber
Journal of Wood Science, 2005Co-Authors: Bruno Clair, Bernard Thibaut, Junji SugiyamaAbstract:The detachment of the gelatinous layer (G-layer), often observed on Microtome cross sections, has led some authors to believe that the G-layer cannot act as the driving force of longitudinal shrinkage in tension wood. The aim of this study was to observe the detachment of the G-layer along fibers. Green wood blocks were cut transversely into two samples. One sample was kept in water and the other was oven-dried. With one face being common to both samples, the detachment of the G-layer was studied on the same fibers. Observations were performed after blocking deformation by embedding. This revealed that the detachment of the G-layer is an effect produced by the act of cutting the transverse face of the wood block to be embedded. At distances greater than 100 µm from this primary surface of the sample, no detachment was observed. Drying shrinkage shows little or no effect on this detachment. The result seems to explain well why the detachment of the G-layer occurs during sectioning using conventional sliding microtomy. These observations prove the adhesion of the G-layer in massive wood and confirm the active role of the G-layer in tension wood properties.
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On the detachment of gelatinous layer in tension wood fiber
Journal of Wood Science, 2005Co-Authors: Bruno Clair, Bernard Thibaut, Junji SugiyamaAbstract:The detachment of gelatinous layer (G-layer), often observed on Microtome cross sections, leads some authors believe that G-layer cannot act as the driving force of longitudinal shrinkage in tension wood. The aim of this study was to observe the detachment of G-layer along fibres. Green wood block was cut transversely into two samples. One sample was kept in water and the other oven-dried. One face being common to both samples, detachment of G-layer has been studied on the same fibres. Observations have been performed after blocking deformation by embedding. It reveals that the detachment of G-layer is a cutting effect produced during the first making of the transverse face of the wood block to be embedded. After 100 µm far from this primary surface of the sample no detachment can be observed. Drying shrinkage does not affect or little this detachment. The result seems to explain well why the detachment of G-layer occurs during sectioning using a conventional sliding microtomy. These observations prove the adhesion of G-layer in massive wood and confirm the active role of G-layer in tension wood properties.