The Experts below are selected from a list of 5598 Experts worldwide ranked by ideXlab platform
Simone Mendonça - One of the best experts on this subject based on the ideXlab platform.
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Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake.
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high Mineral, Fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)
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development of an rp uhplc pda method for quantification of free gossypol in cottonseed cake and fungal treated cottonseed cake
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high Mineral, Fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus.
Aparecido Almeida Conceição - One of the best experts on this subject based on the ideXlab platform.
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Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake.
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high Mineral, Fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)
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development of an rp uhplc pda method for quantification of free gossypol in cottonseed cake and fungal treated cottonseed cake
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high Mineral, Fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus.
Clemente Batista Soares Neto - One of the best experts on this subject based on the ideXlab platform.
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Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake.
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high Mineral, Fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)
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development of an rp uhplc pda method for quantification of free gossypol in cottonseed cake and fungal treated cottonseed cake
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high Mineral, Fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus.
Michel Depaepe - One of the best experts on this subject based on the ideXlab platform.
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airtightness of the window wall interface in cavity brick walls
Energy and Buildings, 2012Co-Authors: Nathan Van Den Bossche, Willem Huyghe, Jan Moens, Arnold Janssens, Michel DepaepeAbstract:Abstract In recent decades there has been an increased focus on enhanced thermal resistance of building components and as a consequence, the relative importance of airtightness on the overall energy losses of buildings has increased significantly. The construction industry requires practical information on the airtightness of individual construction elements and building envelope interfaces. A literature review on the airtightness of window–wall interfaces has shown that no experimental data are available for masonry construction. This paper offers an investigative study on the airtightness of window–wall interfaces of masonry walls, for 13 different installation methods. The results show that the selected solutions cover a wide range of airtightness levels, from 0 m 3 /h m up to 31 m 3 /h m at 50 Pa. The experiments have permitted determining that a very good performance can be obtained by using polyurethane foam and caulking, airtight membranes, polyurethane foam and plywood framing, and plaster and caulking. On the contrary, Mineral Fibre insulation, a partial fill with polyurethane foam and plaster without caulking should be avoided when good airtightness is required. Furthermore, a comprehensive methodology for error calculation is offered, based on error propagation of partially correlated parameters, including the effect of measurement errors, extraneous air leakage and conversion to standard boundary conditions.
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Airtightness of the window–wall interface in cavity brick walls
Energy and Buildings, 2012Co-Authors: Nathan Van Den Bossche, Willem Huyghe, Jan Moens, Arnold Janssens, Michel DepaepeAbstract:Abstract In recent decades there has been an increased focus on enhanced thermal resistance of building components and as a consequence, the relative importance of airtightness on the overall energy losses of buildings has increased significantly. The construction industry requires practical information on the airtightness of individual construction elements and building envelope interfaces. A literature review on the airtightness of window–wall interfaces has shown that no experimental data are available for masonry construction. This paper offers an investigative study on the airtightness of window–wall interfaces of masonry walls, for 13 different installation methods. The results show that the selected solutions cover a wide range of airtightness levels, from 0 m 3 /h m up to 31 m 3 /h m at 50 Pa. The experiments have permitted determining that a very good performance can be obtained by using polyurethane foam and caulking, airtight membranes, polyurethane foam and plywood framing, and plaster and caulking. On the contrary, Mineral Fibre insulation, a partial fill with polyurethane foam and plaster without caulking should be avoided when good airtightness is required. Furthermore, a comprehensive methodology for error calculation is offered, based on error propagation of partially correlated parameters, including the effect of measurement errors, extraneous air leakage and conversion to standard boundary conditions.
José Antônio De Aquino Ribeiro - One of the best experts on this subject based on the ideXlab platform.
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Development of an RP-UHPLC-PDA method for quantification of free gossypol in cottonseed cake and fungal-treated cottonseed cake.
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high Mineral, Fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)
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development of an rp uhplc pda method for quantification of free gossypol in cottonseed cake and fungal treated cottonseed cake
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high Mineral, Fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify cottonseed cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including cottonseed cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol cottonseed treated with a macrofungus.