The Experts below are selected from a list of 3 Experts worldwide ranked by ideXlab platform

Yoshihiro Ohta - One of the best experts on this subject based on the ideXlab platform.

  • time lapse imaging of ca2 induced Swelling and permeability transition single Mitochondrion study
    Archives of Biochemistry and Biophysics, 2019
    Co-Authors: Takahiro Shibata, Mayu Yoneda, Daisuke Morikawa, Yoshihiro Ohta
    Abstract:

    Abstract Mitochondrial functions are closely related to the membrane structure. Mitochondrial Swelling, which is accompanied with dissipation of the crista structure and rupture of the outer membrane, have been observed as mitochondrial damage when mitochondria are under Ca2+-overload or oxidative stress. Although these phenomena have been well studied, the detailed behaviors of individual mitochondria upon Swelling remain unknown. The aim of this study was to investigate the detailed behavior of mitochondrial volume upon addition of Ca2+. Here, we report for the first time, time-lapse measurements of single Mitochondrion Swelling and permeability transition induced by Ca2+ by optical microscopy. We added 220 μM Ca2+ to mitochondria, and found that 1) the Swelling rate depended on the Mitochondrion, 2) a small number of mitochondria showed step-like Swelling, 3) cyclosporin A decreased the percentage of mitochondria that underwent Swelling induced by Ca2+, but did not affect the amplitude of Swelling, 4) permeability transition is necessary but not sufficient for Ca2+-induced Swelling, 5) permeability transition is more sensitive to Ca2+ than Swelling, 6) Ca2+ stimulated mitochondrial Swelling after permeability transition. These results suggest that single Mitochondrion measurement of Swelling is a powerful tool for examining the regulation of mitochondrial structure.

Takahiro Shibata - One of the best experts on this subject based on the ideXlab platform.

  • time lapse imaging of ca2 induced Swelling and permeability transition single Mitochondrion study
    Archives of Biochemistry and Biophysics, 2019
    Co-Authors: Takahiro Shibata, Mayu Yoneda, Daisuke Morikawa, Yoshihiro Ohta
    Abstract:

    Abstract Mitochondrial functions are closely related to the membrane structure. Mitochondrial Swelling, which is accompanied with dissipation of the crista structure and rupture of the outer membrane, have been observed as mitochondrial damage when mitochondria are under Ca2+-overload or oxidative stress. Although these phenomena have been well studied, the detailed behaviors of individual mitochondria upon Swelling remain unknown. The aim of this study was to investigate the detailed behavior of mitochondrial volume upon addition of Ca2+. Here, we report for the first time, time-lapse measurements of single Mitochondrion Swelling and permeability transition induced by Ca2+ by optical microscopy. We added 220 μM Ca2+ to mitochondria, and found that 1) the Swelling rate depended on the Mitochondrion, 2) a small number of mitochondria showed step-like Swelling, 3) cyclosporin A decreased the percentage of mitochondria that underwent Swelling induced by Ca2+, but did not affect the amplitude of Swelling, 4) permeability transition is necessary but not sufficient for Ca2+-induced Swelling, 5) permeability transition is more sensitive to Ca2+ than Swelling, 6) Ca2+ stimulated mitochondrial Swelling after permeability transition. These results suggest that single Mitochondrion measurement of Swelling is a powerful tool for examining the regulation of mitochondrial structure.

Mayu Yoneda - One of the best experts on this subject based on the ideXlab platform.

  • time lapse imaging of ca2 induced Swelling and permeability transition single Mitochondrion study
    Archives of Biochemistry and Biophysics, 2019
    Co-Authors: Takahiro Shibata, Mayu Yoneda, Daisuke Morikawa, Yoshihiro Ohta
    Abstract:

    Abstract Mitochondrial functions are closely related to the membrane structure. Mitochondrial Swelling, which is accompanied with dissipation of the crista structure and rupture of the outer membrane, have been observed as mitochondrial damage when mitochondria are under Ca2+-overload or oxidative stress. Although these phenomena have been well studied, the detailed behaviors of individual mitochondria upon Swelling remain unknown. The aim of this study was to investigate the detailed behavior of mitochondrial volume upon addition of Ca2+. Here, we report for the first time, time-lapse measurements of single Mitochondrion Swelling and permeability transition induced by Ca2+ by optical microscopy. We added 220 μM Ca2+ to mitochondria, and found that 1) the Swelling rate depended on the Mitochondrion, 2) a small number of mitochondria showed step-like Swelling, 3) cyclosporin A decreased the percentage of mitochondria that underwent Swelling induced by Ca2+, but did not affect the amplitude of Swelling, 4) permeability transition is necessary but not sufficient for Ca2+-induced Swelling, 5) permeability transition is more sensitive to Ca2+ than Swelling, 6) Ca2+ stimulated mitochondrial Swelling after permeability transition. These results suggest that single Mitochondrion measurement of Swelling is a powerful tool for examining the regulation of mitochondrial structure.

Daisuke Morikawa - One of the best experts on this subject based on the ideXlab platform.

  • time lapse imaging of ca2 induced Swelling and permeability transition single Mitochondrion study
    Archives of Biochemistry and Biophysics, 2019
    Co-Authors: Takahiro Shibata, Mayu Yoneda, Daisuke Morikawa, Yoshihiro Ohta
    Abstract:

    Abstract Mitochondrial functions are closely related to the membrane structure. Mitochondrial Swelling, which is accompanied with dissipation of the crista structure and rupture of the outer membrane, have been observed as mitochondrial damage when mitochondria are under Ca2+-overload or oxidative stress. Although these phenomena have been well studied, the detailed behaviors of individual mitochondria upon Swelling remain unknown. The aim of this study was to investigate the detailed behavior of mitochondrial volume upon addition of Ca2+. Here, we report for the first time, time-lapse measurements of single Mitochondrion Swelling and permeability transition induced by Ca2+ by optical microscopy. We added 220 μM Ca2+ to mitochondria, and found that 1) the Swelling rate depended on the Mitochondrion, 2) a small number of mitochondria showed step-like Swelling, 3) cyclosporin A decreased the percentage of mitochondria that underwent Swelling induced by Ca2+, but did not affect the amplitude of Swelling, 4) permeability transition is necessary but not sufficient for Ca2+-induced Swelling, 5) permeability transition is more sensitive to Ca2+ than Swelling, 6) Ca2+ stimulated mitochondrial Swelling after permeability transition. These results suggest that single Mitochondrion measurement of Swelling is a powerful tool for examining the regulation of mitochondrial structure.