The Experts below are selected from a list of 321021 Experts worldwide ranked by ideXlab platform
Kelly Velonia - One of the best experts on this subject based on the ideXlab platform.
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Click Chemistry: A Powerful Tool to Create Polymer‐Based Macromolecular Chimeras
Macromolecular Rapid Communications, 2008Co-Authors: Benjamin Le Droumaguet, Kelly VeloniaAbstract:The combination of polymeric with biological materials, to create biohybrid macromolecules that merge the properties of both the natural and synthetic components, is a flourishing area in both lifesciences and biotechnology.Theclick chemistry philosophy hasrecentlyprovided a Powerful Tool in this direction, leading to a plethora of novel, tailor-made biomacromolecules with unprecedented structural characteristics and properties. The different synthetic strategies, using the alkyne‐azide click cycloadditions to bioorthogonally achieve the coupling of synthetic polymers with nucleic acids, peptides, sugars, proteins or even viruses and cells is described. The review covers the latest developments in this very dynamic and rapidly expanding field.
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click chemistry a Powerful Tool to create polymer based macromolecular chimeras
Macromolecular Rapid Communications, 2008Co-Authors: Benjamin Le Droumaguet, Kelly VeloniaAbstract:The combination of polymeric with biological materials, to create biohybrid macromolecules that merge the properties of both the natural and synthetic components, is a flourishing area in both lifesciences and biotechnology.Theclick chemistry philosophy hasrecentlyprovided a Powerful Tool in this direction, leading to a plethora of novel, tailor-made biomacromolecules with unprecedented structural characteristics and properties. The different synthetic strategies, using the alkyne‐azide click cycloadditions to bioorthogonally achieve the coupling of synthetic polymers with nucleic acids, peptides, sugars, proteins or even viruses and cells is described. The review covers the latest developments in this very dynamic and rapidly expanding field.
Benjamin Le Droumaguet - One of the best experts on this subject based on the ideXlab platform.
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Click Chemistry: A Powerful Tool to Create Polymer‐Based Macromolecular Chimeras
Macromolecular Rapid Communications, 2008Co-Authors: Benjamin Le Droumaguet, Kelly VeloniaAbstract:The combination of polymeric with biological materials, to create biohybrid macromolecules that merge the properties of both the natural and synthetic components, is a flourishing area in both lifesciences and biotechnology.Theclick chemistry philosophy hasrecentlyprovided a Powerful Tool in this direction, leading to a plethora of novel, tailor-made biomacromolecules with unprecedented structural characteristics and properties. The different synthetic strategies, using the alkyne‐azide click cycloadditions to bioorthogonally achieve the coupling of synthetic polymers with nucleic acids, peptides, sugars, proteins or even viruses and cells is described. The review covers the latest developments in this very dynamic and rapidly expanding field.
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click chemistry a Powerful Tool to create polymer based macromolecular chimeras
Macromolecular Rapid Communications, 2008Co-Authors: Benjamin Le Droumaguet, Kelly VeloniaAbstract:The combination of polymeric with biological materials, to create biohybrid macromolecules that merge the properties of both the natural and synthetic components, is a flourishing area in both lifesciences and biotechnology.Theclick chemistry philosophy hasrecentlyprovided a Powerful Tool in this direction, leading to a plethora of novel, tailor-made biomacromolecules with unprecedented structural characteristics and properties. The different synthetic strategies, using the alkyne‐azide click cycloadditions to bioorthogonally achieve the coupling of synthetic polymers with nucleic acids, peptides, sugars, proteins or even viruses and cells is described. The review covers the latest developments in this very dynamic and rapidly expanding field.
Rachel Eiges - One of the best experts on this subject based on the ideXlab platform.
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Human Embryonic Stem Cells as a Powerful Tool for Studying Human Embryogenesis
Pediatric Research, 2006Co-Authors: Tamar Dvash, Dalit Ben-yosef, Rachel EigesAbstract:Human embryonic stem cells (HESC) are pluripotent stem cell lines derived from the inner cell mass (ICM) of human blastocyst-stage embryos. They are characterized by their unlimited capacity to self-renew in culture. In addition, they have a broad developmental potential, as demonstrated by their ability to form practically any cell type in vivo and in vitro . These two features have made HESC extremely important in basic and applied research. In addition, they may serve as a Powerful Tool for studying human development. HESC can recapitulate embryogenesis by expressing developmentally regulated genes and by activating molecular pathways as they occur in vivo . Moreover, they can be used to analyze the effect of specific mutations on particular developmental events and may enable us to identify critical factors that play a role in the processes of cell commitment, differentiation, and adult cell reprogramming. Thus, modeling human embryogenesis by the use of HESC may allow new insights into developmental processes, which would otherwise be inaccessible for research.
Heinz E Moser - One of the best experts on this subject based on the ideXlab platform.
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matrix assisted laser desorption ionization time of flight mass spectrometry a Powerful Tool for the mass and sequence analysis of natural and modified oligonucleotides
Nucleic Acids Research, 1993Co-Authors: Uwe Pieles, Werner Zurcher, Martin Schar, Heinz E MoserAbstract:We report the analysis and characterization of natural and modified oligonucleotides by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The present technology was highly improved for this class of compounds by using a new matrix, 2,4,6-trihydroxy acetophenone, together with di- and triammonium salts of organic or inorganic acids to suppress peak broadening due to multiple ion adducts. This methodology can be used in combination with time dependent degradation of oligonucleotides by exonucleases as Powerful Tool to determine sequence compositions.
Mike S. M. Jetten - One of the best experts on this subject based on the ideXlab platform.
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The sequencing batch reactor as a Powerful Tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms
Applied Microbiology and Biotechnology, 1998Co-Authors: Marc Strous, Joseph J. Heijnen, J. Gijs Kuenen, Mike S. M. JettenAbstract:Abstract Currently available microbiological tech- niques are not designed to deal with very slowly growing microorganisms. The enrichment and study of such or- ganisms demands a novel experimental approach. In the present investigation, the sequencing batch reactor (SBR) was applied and optimized for the enrichment and quantitative study of a very slowly growing micro- bial community which oxidizes ammonium anaerobic- ally. The SBR was shown to be a Powerful experimental set-up with the following strong points: (1) e cient biomass retention, (2) a homogeneous distribution of substrates, products and biomass aggregates over the reactor, (3) reliable operation for more than 1 year, and (4) stable conditions under substrate-limiting conditions. Together, these points made possible for the ®rst time the determination of several important physiological parameters such as the biomass yield (0.066 0.01 C-mol/mol ammonium), the maximum speci®c ammo- nium consumption rate (45 5 nmol/mg protein/min) and the maximum speci®c growth rate (0.0027 á h)1, doubling time 11 days). In addition, the persisting stable and strongly selective conditions of the SBR led to a high degree of enrichment (74% of the desired micro- organism). This study has demonstrated that the SBR is a Powerful Tool compared to other techniques used in the past. We suggest that the SBR could be used for the enrichment and quantitative study of a large number of slowly growing microorganisms that are currently out of reach for microbiological research.
