The Experts below are selected from a list of 9090 Experts worldwide ranked by ideXlab platform

Maria Reguera - One of the best experts on this subject based on the ideXlab platform.

  • boron deficiency inhibits root growth by controlling meristem activity under cytokinin regulation
    Plant Science, 2018
    Co-Authors: Laura Pozaviejo, Ildefonso Bonilla, Luis Bolanos, Isidro Abreu, Mary Paz Gonzalezgarcia, Paul Allauca, Maria Reguera
    Abstract:

    Significant advances have been made in the last years trying to identify regulatory pathways that control plant responses to boron (B) deficiency. Still, there is a lack of a deep understanding of how they act regulating growth and development under B limiting conditions. Here, we analyzed the impact of B deficit on cell division leading to root apical meristem (RAM) disorganization. Our results reveal that Inhibition of cell proliferation under the regulatory control of cytokinins (CKs) is an early event contributing to root growth arrest under B deficiency. An early recovery of QC46:GUS expression after transferring B-deficient seedlings to control conditions revealed a role of B in the maintenance of QC identity whose loss under deficiency occurred at later stages of the stress. Additionally, the D-type cyclin CYCD3 overexpressor and triple mutant cycd3;1-3 were used to evaluate the effect on Mitosis Inhibition at the G1-S boundary. Overall, this study supports the hypothesis that meristem activity is inhibited by B deficiency at early stages of the stress as it does cell elongation. Likewise, distinct regulatory mechanisms seem to take place depending on the severity of the stress. The results presented here are key to better understand early signaling responses under B deficiency.

Laura Pozaviejo - One of the best experts on this subject based on the ideXlab platform.

  • boron deficiency inhibits root growth by controlling meristem activity under cytokinin regulation
    Plant Science, 2018
    Co-Authors: Laura Pozaviejo, Ildefonso Bonilla, Luis Bolanos, Isidro Abreu, Mary Paz Gonzalezgarcia, Paul Allauca, Maria Reguera
    Abstract:

    Significant advances have been made in the last years trying to identify regulatory pathways that control plant responses to boron (B) deficiency. Still, there is a lack of a deep understanding of how they act regulating growth and development under B limiting conditions. Here, we analyzed the impact of B deficit on cell division leading to root apical meristem (RAM) disorganization. Our results reveal that Inhibition of cell proliferation under the regulatory control of cytokinins (CKs) is an early event contributing to root growth arrest under B deficiency. An early recovery of QC46:GUS expression after transferring B-deficient seedlings to control conditions revealed a role of B in the maintenance of QC identity whose loss under deficiency occurred at later stages of the stress. Additionally, the D-type cyclin CYCD3 overexpressor and triple mutant cycd3;1-3 were used to evaluate the effect on Mitosis Inhibition at the G1-S boundary. Overall, this study supports the hypothesis that meristem activity is inhibited by B deficiency at early stages of the stress as it does cell elongation. Likewise, distinct regulatory mechanisms seem to take place depending on the severity of the stress. The results presented here are key to better understand early signaling responses under B deficiency.

Li Chen - One of the best experts on this subject based on the ideXlab platform.

  • selective small molecule inhibitor reveals critical mitotic functions of human cdk1
    Proceedings of the National Academy of Sciences of the United States of America, 2006
    Co-Authors: Lyubomir T Vassilev, Christian Tovar, Shaoqing Chen, Dejan Knezevic, Xiaolan Zhao, Hongmao Sun, David C Heimbrook, Li Chen
    Abstract:

    CDK1 is a nonredundant cyclin-dependent kinase (CDK) with an essential role in Mitosis, but its multiple functions still are poorly understood at a molecular level. Here we identify a selective small-molecule inhibitor of CDK1 that reversibly arrests human cells at the G2/M border of the cell cycle and allows for effective cell synchronization in early Mitosis. Inhibition of CDK1 during cell division revealed that its activity is necessary and sufficient for maintaining the mitotic state of the cells, preventing replication origin licensing and premature cytokinesis. Although CDK1 Inhibition for up to 24 h is well tolerated, longer exposure to the inhibitor induces apoptosis in tumor cells, suggesting that selective CDK1 inhibitors may have utility in cancer therapy.

Sergio Capaccioli - One of the best experts on this subject based on the ideXlab platform.

  • induction of apoptosis and Mitosis Inhibition by degraded dna lipotransfection mimicking genotoxic drug effects
    Biochemical and Biophysical Research Communications, 2000
    Co-Authors: Nicola Schiavone, Laura Papucci, Paola Luciani, Andrea Lapucci, Martino Donnini, Sergio Capaccioli
    Abstract:

    Genotoxic damage induces cell cycle arrest and/or apoptosis by activation of p53 oncosuppressor protein. A number of anticancer drugs are genotoxic and their damaging effect upon cells is mediated by this mechanism. Microinjection of defined DNA species directly into nucleus has been reported previously to activate p53 and inhibit cell cycle. Here, we demonstrate that simple addition of heterogeneous degraded DNA to cultured cells (Rat-1 fibroblasts) in combination with lipotransfecting agent DOTAP leads to apoptosis induction and Mitosis Inhibition by a molecular mechanism which mimics that of the cellular response to genotoxic anticancer agents. Indeed, both cellular effects induced by lipotransfected degraded DNA (essentially, heterogeneous small DNA fragments) are associated to p53 activation and modulated by two apoptosis-related genes, such as bcl-2 and c-myc, which also modulate the apoptotic threshold to anticancer agents. Here we raise the hypothesis of exogenous DNA segment lipotransfection as possible new tool for anticancer therapy.

Isidro Abreu - One of the best experts on this subject based on the ideXlab platform.

  • boron deficiency inhibits root growth by controlling meristem activity under cytokinin regulation
    Plant Science, 2018
    Co-Authors: Laura Pozaviejo, Ildefonso Bonilla, Luis Bolanos, Isidro Abreu, Mary Paz Gonzalezgarcia, Paul Allauca, Maria Reguera
    Abstract:

    Significant advances have been made in the last years trying to identify regulatory pathways that control plant responses to boron (B) deficiency. Still, there is a lack of a deep understanding of how they act regulating growth and development under B limiting conditions. Here, we analyzed the impact of B deficit on cell division leading to root apical meristem (RAM) disorganization. Our results reveal that Inhibition of cell proliferation under the regulatory control of cytokinins (CKs) is an early event contributing to root growth arrest under B deficiency. An early recovery of QC46:GUS expression after transferring B-deficient seedlings to control conditions revealed a role of B in the maintenance of QC identity whose loss under deficiency occurred at later stages of the stress. Additionally, the D-type cyclin CYCD3 overexpressor and triple mutant cycd3;1-3 were used to evaluate the effect on Mitosis Inhibition at the G1-S boundary. Overall, this study supports the hypothesis that meristem activity is inhibited by B deficiency at early stages of the stress as it does cell elongation. Likewise, distinct regulatory mechanisms seem to take place depending on the severity of the stress. The results presented here are key to better understand early signaling responses under B deficiency.