The Experts below are selected from a list of 114 Experts worldwide ranked by ideXlab platform
Suraksha Agrawal - One of the best experts on this subject based on the ideXlab platform.
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Characterization of Mixed Lymphocyte Reaction blocking antibodies (MLR-Bf) in human pregnancy
BMC Pregnancy and Childbirth, 2003Co-Authors: Manoj Kumar Pandey, Vijay Saxena, Suraksha AgrawalAbstract:Background It is known that during normal pregnancy and after immunotherapy blocking antibodies are developed, these antibodies inhibit Mixed Lymphocyte Reaction and are also anti-mitogenic in nature. Mixed Lymphocyte Reaction blocking antibodies are specific to the husband's Lymphocytes. In the present study an attempt has been made to characterize the Mixed Lymphocyte Reaction blocking antibodies in normal pregnancy and in women with recurrent spontaneous abortion after immunotherapy. Methods Serum was obtained from women of different gestational windows of pregnancy (Ist, IInd, IIIrd trimesters and post delivery period of normal pregnancy), recurrent spontaneous aborters from pre and post immunization. Healthy (male and females) controls were screened for the presence of Mixed Lymphocyte Reaction blocking antibodies. The standard Mixed Lymphocyte Reaction technique was used to evaluate the inhibitory effect of serum in the Mixed Lymphocyte Reaction. Each serum was tested for cytotoxic antibodies. Immunoglobulin G and its isotypes were isolated according to the standard protocol. Results In the present study we have observed that there was significant inhibition of proliferation response when immunoglobulin G from different trimesters of pregnancy were added to one way Mixed Lymphocyte Reaction or to phytohemagglutinin activated Lymphocyte proliferation assay. Similar pattern was seen when immunoglobulin G isolated from adequately immunized women with recurrent spontaneous abortion was used. It was further confirmed that amongst all the isotypes of immunoglobulin G, only immunoglobulin G-3 was found to be positive for the inhibitory effect. Conclusions Present study indicates that Mixed Lymphocyte Reaction blocking antibodies are immunoglobulin G-3 in nature. It is developed during pregnancy and also after immunotherapy in women with recurrent spontaneous abortion who subsequently have the successful pregnancy.
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characterization of Mixed Lymphocyte Reaction blocking antibodies mlr bf in human pregnancy
BMC Pregnancy and Childbirth, 2003Co-Authors: Manoj Kumar Pandey, Vijay Saxena, Suraksha AgrawalAbstract:Background It is known that during normal pregnancy and after immunotherapy blocking antibodies are developed, these antibodies inhibit Mixed Lymphocyte Reaction and are also anti-mitogenic in nature. Mixed Lymphocyte Reaction blocking antibodies are specific to the husband's Lymphocytes. In the present study an attempt has been made to characterize the Mixed Lymphocyte Reaction blocking antibodies in normal pregnancy and in women with recurrent spontaneous abortion after immunotherapy.
Manoj Kumar Pandey - One of the best experts on this subject based on the ideXlab platform.
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Characterization of Mixed Lymphocyte Reaction blocking antibodies (MLR-Bf) in human pregnancy
BMC Pregnancy and Childbirth, 2003Co-Authors: Manoj Kumar Pandey, Vijay Saxena, Suraksha AgrawalAbstract:Background It is known that during normal pregnancy and after immunotherapy blocking antibodies are developed, these antibodies inhibit Mixed Lymphocyte Reaction and are also anti-mitogenic in nature. Mixed Lymphocyte Reaction blocking antibodies are specific to the husband's Lymphocytes. In the present study an attempt has been made to characterize the Mixed Lymphocyte Reaction blocking antibodies in normal pregnancy and in women with recurrent spontaneous abortion after immunotherapy. Methods Serum was obtained from women of different gestational windows of pregnancy (Ist, IInd, IIIrd trimesters and post delivery period of normal pregnancy), recurrent spontaneous aborters from pre and post immunization. Healthy (male and females) controls were screened for the presence of Mixed Lymphocyte Reaction blocking antibodies. The standard Mixed Lymphocyte Reaction technique was used to evaluate the inhibitory effect of serum in the Mixed Lymphocyte Reaction. Each serum was tested for cytotoxic antibodies. Immunoglobulin G and its isotypes were isolated according to the standard protocol. Results In the present study we have observed that there was significant inhibition of proliferation response when immunoglobulin G from different trimesters of pregnancy were added to one way Mixed Lymphocyte Reaction or to phytohemagglutinin activated Lymphocyte proliferation assay. Similar pattern was seen when immunoglobulin G isolated from adequately immunized women with recurrent spontaneous abortion was used. It was further confirmed that amongst all the isotypes of immunoglobulin G, only immunoglobulin G-3 was found to be positive for the inhibitory effect. Conclusions Present study indicates that Mixed Lymphocyte Reaction blocking antibodies are immunoglobulin G-3 in nature. It is developed during pregnancy and also after immunotherapy in women with recurrent spontaneous abortion who subsequently have the successful pregnancy.
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characterization of Mixed Lymphocyte Reaction blocking antibodies mlr bf in human pregnancy
BMC Pregnancy and Childbirth, 2003Co-Authors: Manoj Kumar Pandey, Vijay Saxena, Suraksha AgrawalAbstract:Background It is known that during normal pregnancy and after immunotherapy blocking antibodies are developed, these antibodies inhibit Mixed Lymphocyte Reaction and are also anti-mitogenic in nature. Mixed Lymphocyte Reaction blocking antibodies are specific to the husband's Lymphocytes. In the present study an attempt has been made to characterize the Mixed Lymphocyte Reaction blocking antibodies in normal pregnancy and in women with recurrent spontaneous abortion after immunotherapy.
Vijay Saxena - One of the best experts on this subject based on the ideXlab platform.
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Characterization of Mixed Lymphocyte Reaction blocking antibodies (MLR-Bf) in human pregnancy
BMC Pregnancy and Childbirth, 2003Co-Authors: Manoj Kumar Pandey, Vijay Saxena, Suraksha AgrawalAbstract:Background It is known that during normal pregnancy and after immunotherapy blocking antibodies are developed, these antibodies inhibit Mixed Lymphocyte Reaction and are also anti-mitogenic in nature. Mixed Lymphocyte Reaction blocking antibodies are specific to the husband's Lymphocytes. In the present study an attempt has been made to characterize the Mixed Lymphocyte Reaction blocking antibodies in normal pregnancy and in women with recurrent spontaneous abortion after immunotherapy. Methods Serum was obtained from women of different gestational windows of pregnancy (Ist, IInd, IIIrd trimesters and post delivery period of normal pregnancy), recurrent spontaneous aborters from pre and post immunization. Healthy (male and females) controls were screened for the presence of Mixed Lymphocyte Reaction blocking antibodies. The standard Mixed Lymphocyte Reaction technique was used to evaluate the inhibitory effect of serum in the Mixed Lymphocyte Reaction. Each serum was tested for cytotoxic antibodies. Immunoglobulin G and its isotypes were isolated according to the standard protocol. Results In the present study we have observed that there was significant inhibition of proliferation response when immunoglobulin G from different trimesters of pregnancy were added to one way Mixed Lymphocyte Reaction or to phytohemagglutinin activated Lymphocyte proliferation assay. Similar pattern was seen when immunoglobulin G isolated from adequately immunized women with recurrent spontaneous abortion was used. It was further confirmed that amongst all the isotypes of immunoglobulin G, only immunoglobulin G-3 was found to be positive for the inhibitory effect. Conclusions Present study indicates that Mixed Lymphocyte Reaction blocking antibodies are immunoglobulin G-3 in nature. It is developed during pregnancy and also after immunotherapy in women with recurrent spontaneous abortion who subsequently have the successful pregnancy.
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characterization of Mixed Lymphocyte Reaction blocking antibodies mlr bf in human pregnancy
BMC Pregnancy and Childbirth, 2003Co-Authors: Manoj Kumar Pandey, Vijay Saxena, Suraksha AgrawalAbstract:Background It is known that during normal pregnancy and after immunotherapy blocking antibodies are developed, these antibodies inhibit Mixed Lymphocyte Reaction and are also anti-mitogenic in nature. Mixed Lymphocyte Reaction blocking antibodies are specific to the husband's Lymphocytes. In the present study an attempt has been made to characterize the Mixed Lymphocyte Reaction blocking antibodies in normal pregnancy and in women with recurrent spontaneous abortion after immunotherapy.
Masahiro Nishibori - One of the best experts on this subject based on the ideXlab platform.
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advanced glycation end products enhance monocyte activation during human Mixed Lymphocyte Reaction
Clinical Immunology, 2010Co-Authors: Katsuhisa Ohashi, Hideo Takahashi, Hiroshi Sadamori, Takahito Yagi, Tadashi Yoshino, Masahiro Nishibori, Shuji Mori, Hidenori Wake, Hiroaki Matsuda, Noriaki TanakaAbstract:Abstract Posttransplant diabetes mellitus (PTDM) is a frequent complication among transplant recipients. Ligation of advanced glycation end products (AGEs) with their receptor (RAGE) on monocytes/macrophages plays roles in the diabetes complications. The enhancement of adhesion molecule expression on monocytes/macrophages activates T-cells, leading to reduced allograft survival. We investigated the effect of four distinct AGE subtypes (AGE-2/AGE-3/AGE-4/AGE-5) on the expressions of intracellular adhesion molecule (ICAM)-1, B7.1, B7.2 and CD40 on monocytes, the production of interferon (IFN)-γ and tumor necrosis factor (TNF)-α and the proliferation of T-cells during human Mixed Lymphocyte Reaction (MLR). AGE-2 and AGE-3 selectively induced the adhesion molecule expression, cytokine production and T-cell proliferation. The AGE-induced up-regulation of adhesion molecule expression was involved in the cytokine production and T-cell proliferation. AGE-2 and AGE-3 up-regulated the expression of RAGE on monocytes; therefore, the AGEs may activate monocytes, leading to the up-regulation of adhesion molecule expression, cytokine production and T-cell proliferation.
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effect of adenosine receptor subtypes stimulation on Mixed Lymphocyte Reaction
European Journal of Pharmacology, 2007Co-Authors: Hideo Takahashi, Hiromi Iwagaki, Ryosuke Hamano, Toru Kanke, Hiroshi Sadamori, Takahito Yagi, Tadashi Yoshino, Toshiaki Sendo, Noriaki Tanaka, Masahiro NishiboriAbstract:Abstract The cell-to-cell interaction through binding of intercellular adhesion molecule (ICAM)-1 on monocytes to their ligands Lymphocyte function-associated antigen (LFA)-1 on T-cells plays important roles in cytokine production and T-cell proliferation. Interleukin (IL)-18, which plasma levels are elevated in patients during acute rejection following organ transplantation, induces the expression of ICAM-1 on monocytes, production of interferon (IFN)-γ and IL-12 and Lymphocyte proliferation during human Mixed Lymphocyte Reaction. Activation of the adenosine A 2A receptor on during reperfusion of various tissues has been found to markedly reduce ischemia–reperfusion injury. In the present study, we examined the effect of adenosine at increasing concentrations ranging from 0.1 to 100 μM on the IL-18-enhanced expression of ICAM-1, production of IFN-γ and IL-12 and Lymphocyte proliferation during human Mixed Lymphocyte Reaction. Adenosine inhibited the IL-18-initiated immune responses. The IC 50 values of adenosine for inhibition of the IL-18-enhanced ICAM-1 expression, IFN-γ production and Lymphocyte proliferation were 20 μM, respectively. The actions of adenosine depended on the stimulation of adenosine A 2A receptor. An inhibitor of protein kinase A (PKA) at 100 μM inhibited the actions of adenosine, suggesting that PKA might be involved in the actions of adenosine. On the other hand, the stimulation of adenosine A 1 and A 3 receptor blocked the actions of adenosine A 2A receptor stimulation. These results suggest that adenosine inhibits the immune responses during Mixed Lymphocyte Reaction via adenosine A 2A receptor.
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the immunosuppressive effects of nicotine during human Mixed Lymphocyte Reaction
European Journal of Pharmacology, 2007Co-Authors: Hideo Takahashi, Hiromi Iwagaki, Ryosuke Hamano, Toru Kanke, Hiroshi Sadamori, Takahito Yagi, Tadashi Yoshino, Noriaki Tanaka, Masahiro NishiboriAbstract:Abstract Cell-to-cell interaction through binding intercellular adhesion molecule (ICAM)-1, B7.1, B7.2 and CD40 on monocytes and their ligands on T-cells plays roles in cytokine production and T-cell proliferation. Interleukin (IL)-18, which is elevated in the plasma during acute rejection after organ transplantation, induces the expression of ICAM-1, B7.1, B7.2 and CD40, production of interferon (IFN)-γ and IL-12 and proliferation of Lymphocytes during human Mixed Lymphocyte Reaction. Nicotine is known to inhibit the production of pro-inflammatory cytokines from macrophages through the stimulation of nicotinic acetylcholine receptor α7 subunit. In the present study, we examined the effect of increasing concentrations ranging from 0.1 to 100 μM of nicotine on the expression of ICAM-1, B7.1, B7.2 and CD40, production of IFN-γ and IL-12 and proliferation of Lymphocytes during Mixed Lymphocyte Reaction treated with IL-18 at 100 ng/ml for 48 h. Nicotine inhibited the expression of adhesion molecules, cytokine production and Lymphocyte proliferation. The IC50 values of nicotine for inhibition of the IL-18-enhanced ICAM-1 expression, IFN-γ production and proliferation were 1, 1 and 2 μM, respectively. A non-selective and a selective antagonist for nicotinic acetylcholine receptor α7 subunit, mecamylamine and α-bungarotoxin abolished the effects of nicotine. The actions of nicotine might depend on stimulation of nicotinic acetylcholine receptor α7 subunit. Nicotine induced prostaglandin E2 production during Mixed Lymphocyte Reaction. The inhibitors of cyclooxygenase (COX)-2 and protein kinase A (PKA) at 100 μM inhibited the actions of nicotine, suggesting that the endogenous prostaglandin E2 might be, at least, partially involved the actions of nicotine.
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the immunosuppressive effects of ciprofloxacin during human Mixed Lymphocyte Reaction
Clinical Immunology, 2006Co-Authors: Goutarou Katsuno, Hideo Takahashi, Hiromi Iwagaki, Takahito Yagi, Tadashi Yoshino, Masahiro Nishibori, Shuji Mori, Kenji Mizuno, Shinya Saito, Noriaki TanakaAbstract:Abstract Interleukin (IL)-18, which is elevated in the plasma during acute rejection after organ transplantation, is known to induce the expression of intercellular adhesion molecule (ICAM)-1, B7.1, B7.2, CD40 and CD40 ligand (CD40L) on monocytes, the production of interferon (IFN)-γ and IL-12 and the proliferation of Lymphocytes during the human Mixed Lymphocyte Reaction (MLR). Ciprofloxacin (CIP), which is useful for the clinical treatment of infections due to its antibacterial properties after transplantation, was shown to suppress the IFN-γ and IL-12 production, the Lymphocyte proliferation and the ICAM-1, B7.1, B7.2 and CD40 expression on monocytes during MLR in the presence of IL-18. CIP also induced the production of prostaglandin (PG) E2. In order to determine whether the effects of CIP on the expression of the activation markers were due to CIP-dependent production of PGE2, we examined the effect of cyclooxygenase (COX)-2 and protein kinase A (PKA) inhibitors on the actions of CIP. Thereby, the inhibitors were found to abolish the actions of CIP. These results therefore suggest that CIP might exert its immune modulatory effects via the production of PGE2.
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prostaglandin e1 initiated immune regulation during human Mixed Lymphocyte Reaction
Clinical Immunology, 2005Co-Authors: Hideo Takahashi, Hiromi Iwagaki, Takahito Yagi, Tadashi Yoshino, Masahiro Nishibori, Ryuji Tamura, Goutarou Katsuno, Shuji Mori, Noriaki TanakaAbstract:Abstract Prostaglandin E1 (PGE1) has therapeutic value for transplantations due to its microvascular activity. Interleukin (IL)-18, which is elevated in plasma during the acute rejection after organ transplantation, elicits the expression of intercellular adhesion molecule (ICAM)-1, B7.1, B7.2, CD40, and CD40 ligand (CD40L) on monocytes as well as the production of interferon (IFN)-γ and IL-12 and proliferation of T-cells during the human Mixed Lymphocyte Reaction (MLR) in an in vitro model of acute rejection. In contrast, PGE1 inhibits all the adhesion molecule expression, cytokine production and T-cell proliferation in the presence of IL-18. The effects of PGE1 depend on stimulation of the IP/EP2/EP4-receptor, and thus, PGE1 might have therapeutic potential for treating acute rejection due to its immune regulatory effect.
Marian L Kruzel - One of the best experts on this subject based on the ideXlab platform.
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lactoferrin regulates proliferative response of human peripheral blood mononuclear cells to phytohemagglutinin and Mixed Lymphocyte Reaction
Archivum Immunologiae Et Therapiae Experimentalis, 2001Co-Authors: Michal Zimecki, D Stepniak, A Szynol, Marian L KruzelAbstract:The aim of this study was to investigate the effects of lactoferrin (LF) on the proliferative response of h uman peripheral blood mononuclear cells (PBMC) induced by phytohemagglutinin (PHA) and all oantigens in a two-way Mixed Lymphocyte Reaction (MLR). The proliferative responses were measured by MTT colorimetric assay and presented as a proliferation index (PI) or as changes in PI caused by LF relative to PHA or MLR controls. We found that the effects of LF in both experimental models were differential and dependent on an i ndividual PBMC reactivity, mitogen or alloantigen and LF concentration. Generally, Lymphocytes from donors responsive to LF exhibited higher proliferation indices to PHA when compared with non-responsive individuals. L actoferrin at low doses showed regulatory effects, whereas at higher doses the proliferation was significantly reduced. Mixed Lymphocyte Reaction was generally inhibited by LF. The results suggest that t he differential action o f LF might be due to its ability to sense the activation status of Lymphocytes.
