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Alla V Silina - One of the best experts on this subject based on the ideXlab platform.

  • association of the scalloppatinopecten Yessoensisand epibiotic barnaclebalanus rostratus inter specific interactions and trophic relationships determined by fatty acid analysis
    Marine Ecology, 2016
    Co-Authors: Alla V Silina, Natalia V Zhukova
    Abstract:

    Marine mollusks provide shelter to epibiotic organisms which settle on the outer surface of their hard shells. Epibionts can exert beneficial or detrimental effects on the host mollusk. In this study, the ecology of the association of the commercially valuable marine mobile scallop Patinopecten (=Mizuhopecten) Yessoensis with its epibiotic barnacle Balanus rostratus was investigated. Fatty acid analysis was performed to determine the trophic relationships between these species. The distribution of fatty acid markers in this scallop species suggests an important contribution of diatoms, flagellates and animal material in its diet. The fatty acid analysis indicated a predominance of diatoms in the diet of the adult barnacles and some detrital input into the diets of young individuals. It was found that adult barnacles may compete with the scallops for food sources such as diatoms and zooplankton. It was revealed that with a negligible biomass of epibionts, the interactions between the barnacle and scallop may be regarded as commensalisms. An increase in the weight of epibiotic barnacles resulted in decreases in the weights and shell heights of the scallops, testifying to the adverse influence of a high biomass of epibionts on their host.

  • population dynamics of the japanese scallop Mizuhopecten Yessoensis bivalvia under conditions of enhanced hydrodynamics
    Russian Journal of Marine Biology, 2005
    Co-Authors: Alla V Silina, Yu Ya Latypov
    Abstract:

    A population of the Japanese scallop, Mizuhopecten Yessoensis, found in the open area of Peter the Great Bay (Sea of Japan), which was not protected against southeastern summer monsoons, has been studied. Specimens of different ages, from 1 year old to 11 years old, were found in the population. According to a provisional estimation, the total reserve of the Japanese scallop in the population reached 30 000 specimens. After strong typhoons, up to 6 000 specimens were stranded along the shoreline. Even after repeatedly occurring typhoons observed during one particular summer season, the population of M. Yessoensis remained rather stable, although its age structure demonstrated both uneven annual recruitment in the population and different survival rates in different generations. Specimens of the highly productive generation of 1999 constituted the bulk of the bottom population and coastal wreckage. At older ages, the probability for the Japanese scallop to be stranded during a storm decreases significantly.

  • long term variation of polychaete bioerosion of the scallop Mizuhopecten Yessoensis from the northwest part of the sea of japan
    Russian Journal of Marine Biology, 2003
    Co-Authors: Alla V Silina
    Abstract:

    The bioerosion of shells of the Japanese scallop Mizuhopecten Yessoensis by endolithic organisms over the last few decades and millenia in areas close to the main sources of organic pollution in Peter the Great Bay was studied. It was established that the occurrence of Japanese scallop shells damaged by borers increased over 2500 years practically from 0 up to 96% in the northern part of the bay at Mys Peschany Cape, which is subject to the impact of the Pazdol'naya River drain. The bioerosion of the Japanese scallop shells has increased considerably over the last two decades in the coastal zone, near the center of the city of Vladivostok, at one of the main sources of the city's wastewater discharge. Thus, in 4-year old individuals 33.0 ± 2.9% of upper valves were eroded on average in 1982; in 1998 it was already 68.3 ± 6.4%. In Reinike strait, that is, at some distance from the mouth of Razdol'naya River and from the main discharge sources of polluted waters of Vladivostok, the shells of the Japanese scallop were eroded to a lesser extent than in the population from the coastal region near the city. However, a significant increase in the degree of bioerosion of the scallop shells was observed over one-and-a-half decades: in 1987 in 4-year scallops 2.3 ± 0.3% of the area of the upper valve was eroded on average, and in 1999 that parameter increased and became 32.6 ± 3.4%. In the western part of Peter the Great Bay near Furugelm Island, which is located 20 km away from the mouth of the Tumannaya River, the degree of bioerosion of scallop shells had also increased significantly over the last 30 years. The increase in the degree of bioerosion of the shell is connected to a gradual increase in the content of organic substances in the bottom sediments. This is a factor favorable for the development of bacteria and phytoplankton, which is a food source for polychaetes—the basic shell boring symbionts of the Japanese scallop.

  • seasonal variability in the levels of fe zn cu mn and cd in the hepatopancreas of the japanese scallop Mizuhopecten Yessoensis
    Russian Journal of Marine Biology, 2002
    Co-Authors: N N Belcheva, Alla V Silina, E N Slinko, V P Chelomin
    Abstract:

    The relationships between concentrations (μg/g of tissue) and content (μg/organ) of the metals Fe, Zn, Cu, Mn, and Cd with seasonal changes in the weight of the hepatopancreas in the Japanese scallop Mizuhopecten Yessoensis are studied. It is shown that, during a year, the weight of the hepatopancreas in the mollusk shows significant (more than twofold) changes. A positive correlation is revealed between the seasonal changes in the concentrations of the physiologically important metals Fe, Zn, Cu, and Mn and the weight of this organ, although no general consistent pattern of seasonal variability in the metal concentrations is found. For toxic Cd, a negative correlation is found between its concentration and the weight of the hepatopancreas.

  • mortality of late juvenile and adult stages of the scallop Mizuhopecten Yessoensis jay
    Aquaculture, 1996
    Co-Authors: Alla V Silina
    Abstract:

    Abstract Age and size distributions of living populations of the Japanese scallop Mizuhopecten Yessoensis (Jay) and accumulations of dead scallop shells were compared in two areas of Peter the Great Bay (northwestern Sea of Japan) to determine the life stages at which higher mortality occurs. The highest mortality was recorded in the juvenile and pre-mature stages, when scallops were less than 2 years of age, and for individuals 6–7 years of age (probably the beginning of the senile period of scallop development) and 9–10 years of age (transition to the old-aged stage). The possible seasons of death were determined by examining the microsculpture of the external shell surface. The seasons at which the highest mortality occurred differed in various localities and was dependent on the extent of deviation of environmental characteristics from the optimal values for the scallop.

Isao Kudo - One of the best experts on this subject based on the ideXlab platform.

  • nitrogen stable isotopes reveal age dependent dietary shift in the japanese scallop Mizuhopecten Yessoensis
    Isotopes in Environmental and Health Studies, 2017
    Co-Authors: Frolan A Aya, Isao Kudo
    Abstract:

    Ontogenetic niche shifts in diet are a consequence of changes in body size or resource partitioning between age classes. To better resolve the feeding patterns of the Japanese scallop Mizuhopecten Yessoensis, we examined the relative importance of age and size in the diet of this species using stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) from 2006 to 2009. Contribution of food sources was quantified using an isotope mixing model by comparing the muscle tissue isotope ratios to those of suspended particulate organic matter (SPOM) and their zooplankton prey (e.g. micro- and meso-zooplankton). Unlike the δ13C values, which remained constant with age and size, muscle δ15N values were more positively correlated with age accounting for 69 % of variations than size with only 46 %. Increasing 15N values with age suggested that shifts in diet from SPOM to micro- and meso-zooplankton occurred during ontogeny in M. Yessoensis. Results of the isotope mixing model indicated that SPOM contribution to scallop's diet decreased from 68 to 8 % while those of zooplankton increased from 15 to 50 % with increasing age. This study concludes that age-related dietary shift explains the enrichment of 15N, as a result of predation on zooplankton by M. Yessoensis.

  • Isotopic shifts with size, culture habitat, and enrichment between the diet and tissues of the Japanese scallop Mizuhopecten Yessoensis (Jay, 1857)
    Marine Biology, 2010
    Co-Authors: Frolan A Aya, Isao Kudo
    Abstract:

    Use of stable isotope signatures to trace diet patterns in cultured marine bivalves, particularly when changing culture habitat, requires knowledge of the isotopic shift and enrichment between diet and consumer’s tissues. The aim of this study was to determine the patterns of isotope change and the variability of enrichment values (∆δ^13C and ∆δ^15N) in different tissues (muscle, gonad, digestive gland) of the Japanese scallop ( Mizuhopecten Yessoensis ). It was hypothesized that the isotopic signatures of a consumer’s tissues changed during settlement and that the changes were related to variations in the isotopic signatures of food sources and gut contents. Particular attention was paid to the isotope enrichment between the diet and a consumer’s tissues using isotope analysis of gut content. Muscle δ^15N values decreased significantly 3–5 months post-settlement in a nearshore seabed, concomitant with the ingestion of lower δ^15N food. For juvenile scallops, sinking particles (SP) were considered a more important dietary source than suspended particulate organic matter (SPOM), based on the correspondence between SP and gut contents δ^13C. Enrichment values (∆δ^13C and ∆δ^15N) varied with tissue and season. ∆δ^15N was 2.4‰ in muscle, 1.2‰ in gonad, and 0.7‰ in the digestive gland. ∆δ^13C was 3.2‰ in muscle, 2.3‰ in gonad, and −0.5‰ in the digestive gland. ∆δ^15N was the lowest in summer (0.3‰), and ∆δ^13C was the highest in autumn (2.8‰). ∆δ^15N values were significantly influenced by age, but not ∆δ^13C. Patterns of isotope ratios and enrichment values may be related to physiological attributes and differences in diet. This is the first study to demonstrate isotopic shift and enrichment encountered in different tissues of a cultured scallop when changing culture habitat.

  • isotopic determination of japanese scallop patinopecten Mizuhopecten Yessoensis jay tissues shows habitat related differences in food sources
    Journal of Shellfish Research, 2007
    Co-Authors: Frolan A Aya, Isao Kudo
    Abstract:

    Abstract Using stable isotope analysis, this study examined the potential food sources of the Japanese scallop, Patinopecten (Mizuhopecten) Yessoensis (Jay) and determined whether the isotopic ratios inferred from tissues of cultured scallops varied according to culture systems (hanging and sowing). Different tissues (i.e., muscle, ctenidia, and digestive gland) of scallops nesting in Saloma Lake (18.3–113.0 mm shell height) and Tokoro (66.8–121.4 mm shell height) and their potential food sources were analyzed for stable carbon and nitrogen isotope ratios. Average isotope ratios obtained in scallop muscle and ctenidia (−20.6 and −21.0‰ for δ13C; 3.3 and 3.0‰ for δ15N) from Tokoro area (sowing culture; ∼65 m depth) had close affinities to suspended particulate organic matter (−21.6‰ and 2.7‰ for δ13C and δ15N, respectively) suggesting that the scallops from Tokoro area depends on detritus as an important food source. Saloma Lake (hanging culture; ∼20 m depth) scallop muscle and ctenidia tissues however had...

Frolan A Aya - One of the best experts on this subject based on the ideXlab platform.

  • nitrogen stable isotopes reveal age dependent dietary shift in the japanese scallop Mizuhopecten Yessoensis
    Isotopes in Environmental and Health Studies, 2017
    Co-Authors: Frolan A Aya, Isao Kudo
    Abstract:

    Ontogenetic niche shifts in diet are a consequence of changes in body size or resource partitioning between age classes. To better resolve the feeding patterns of the Japanese scallop Mizuhopecten Yessoensis, we examined the relative importance of age and size in the diet of this species using stable isotope ratios of carbon (δ13C) and nitrogen (δ15N) from 2006 to 2009. Contribution of food sources was quantified using an isotope mixing model by comparing the muscle tissue isotope ratios to those of suspended particulate organic matter (SPOM) and their zooplankton prey (e.g. micro- and meso-zooplankton). Unlike the δ13C values, which remained constant with age and size, muscle δ15N values were more positively correlated with age accounting for 69 % of variations than size with only 46 %. Increasing 15N values with age suggested that shifts in diet from SPOM to micro- and meso-zooplankton occurred during ontogeny in M. Yessoensis. Results of the isotope mixing model indicated that SPOM contribution to scallop's diet decreased from 68 to 8 % while those of zooplankton increased from 15 to 50 % with increasing age. This study concludes that age-related dietary shift explains the enrichment of 15N, as a result of predation on zooplankton by M. Yessoensis.

  • Isotopic shifts with size, culture habitat, and enrichment between the diet and tissues of the Japanese scallop Mizuhopecten Yessoensis (Jay, 1857)
    Marine Biology, 2010
    Co-Authors: Frolan A Aya, Isao Kudo
    Abstract:

    Use of stable isotope signatures to trace diet patterns in cultured marine bivalves, particularly when changing culture habitat, requires knowledge of the isotopic shift and enrichment between diet and consumer’s tissues. The aim of this study was to determine the patterns of isotope change and the variability of enrichment values (∆δ^13C and ∆δ^15N) in different tissues (muscle, gonad, digestive gland) of the Japanese scallop ( Mizuhopecten Yessoensis ). It was hypothesized that the isotopic signatures of a consumer’s tissues changed during settlement and that the changes were related to variations in the isotopic signatures of food sources and gut contents. Particular attention was paid to the isotope enrichment between the diet and a consumer’s tissues using isotope analysis of gut content. Muscle δ^15N values decreased significantly 3–5 months post-settlement in a nearshore seabed, concomitant with the ingestion of lower δ^15N food. For juvenile scallops, sinking particles (SP) were considered a more important dietary source than suspended particulate organic matter (SPOM), based on the correspondence between SP and gut contents δ^13C. Enrichment values (∆δ^13C and ∆δ^15N) varied with tissue and season. ∆δ^15N was 2.4‰ in muscle, 1.2‰ in gonad, and 0.7‰ in the digestive gland. ∆δ^13C was 3.2‰ in muscle, 2.3‰ in gonad, and −0.5‰ in the digestive gland. ∆δ^15N was the lowest in summer (0.3‰), and ∆δ^13C was the highest in autumn (2.8‰). ∆δ^15N values were significantly influenced by age, but not ∆δ^13C. Patterns of isotope ratios and enrichment values may be related to physiological attributes and differences in diet. This is the first study to demonstrate isotopic shift and enrichment encountered in different tissues of a cultured scallop when changing culture habitat.

  • isotopic determination of japanese scallop patinopecten Mizuhopecten Yessoensis jay tissues shows habitat related differences in food sources
    Journal of Shellfish Research, 2007
    Co-Authors: Frolan A Aya, Isao Kudo
    Abstract:

    Abstract Using stable isotope analysis, this study examined the potential food sources of the Japanese scallop, Patinopecten (Mizuhopecten) Yessoensis (Jay) and determined whether the isotopic ratios inferred from tissues of cultured scallops varied according to culture systems (hanging and sowing). Different tissues (i.e., muscle, ctenidia, and digestive gland) of scallops nesting in Saloma Lake (18.3–113.0 mm shell height) and Tokoro (66.8–121.4 mm shell height) and their potential food sources were analyzed for stable carbon and nitrogen isotope ratios. Average isotope ratios obtained in scallop muscle and ctenidia (−20.6 and −21.0‰ for δ13C; 3.3 and 3.0‰ for δ15N) from Tokoro area (sowing culture; ∼65 m depth) had close affinities to suspended particulate organic matter (−21.6‰ and 2.7‰ for δ13C and δ15N, respectively) suggesting that the scallops from Tokoro area depends on detritus as an important food source. Saloma Lake (hanging culture; ∼20 m depth) scallop muscle and ctenidia tissues however had...

Akira Miyazono - One of the best experts on this subject based on the ideXlab platform.

  • relationship between spat density food availability and growth of spawners in cultured Mizuhopecten Yessoensis in funka bay concurrence with el nino southern oscillation
    Canadian Journal of Fisheries and Aquatic Sciences, 2009
    Co-Authors: Katsuhisa Baba, Rieko Sugawarar Sugawara, Hisashi Nittah Nitta, Kiyoshi Endouk Endou, Akira Miyazono
    Abstract:

    To elucidate the factors that influence the interannual variation in the density of cultured Japanese scallop (Mizuhopecten Yessoensis) spat, we analyzed the relationship between spat density (Ds),...

  • gis based multi criteria evaluation models for identifying suitable sites for japanese scallop Mizuhopecten Yessoensis aquaculture in funka bay southwestern hokkaido japan
    Aquaculture, 2008
    Co-Authors: Nyoman I Radiarta, Sei-ichi Saitoh, Akira Miyazono
    Abstract:

    Abstract Production from Japanese scallop (Mizuhopecten Yessoensis) aquaculture is increasing, and supports coastal communities. To ensure both success and long-term sustainability of providing scallop productions, finding suitable sites is an important step in any aquaculture operation. This study was conducted to identify the most suitable sites for hanging culture of Japanese scallop using geographic information system (GIS)-based multi-criteria evaluation models. Remote sensing data (Sea-viewing Wide Field-of-view Sensor (SeaWiFS), Moderate Resolution Imaging Spectroradiometer (MODIS) and Advanced Land Observing Satellite (ALOS)) were used to extract most of the parameters. Seven thematic layers were grouped into two basic requisite for scallop aquaculture, namely biophysical (sea temperature, chlorophyll, suspended sediment and bathymetry) and social–infrastructural (distance to town, pier and land-based facilities). A constraint layer was used to exclude the areas from suitability maps that cannot be allowed to develop scallop aquaculture, including harbor, area near town/industrial and river mouth. A series of GIS models was developed to identify the most suitable areas for scallop culture using multi-criteria evaluation known as weighted linear combination. Suitability scores were ranked on a scale from 1 (least suitable) to 8 (most suitable), and about 56% of the total potential area with bottom depths less than 60 m had the higher scores (scores 7 and 8). These areas were shown to have the optimum condition for scallop culture in this region. The final suitability model outputs were compared with field verification data and found to be consistent.

  • occurrence and detrimental effects of the bivalve inhabiting hydroid eutima japonica on juveniles of the japanese scallop Mizuhopecten Yessoensis in funka bay japan relationship to juvenile massive mortality in 2003
    Marine Biology, 2007
    Co-Authors: Katsuhisa Baba, Akira Miyazono, Keiji Matsuyama, Shoui Kohno, Shin Kubota
    Abstract:

    In November 2003, we first observed prevalent occurrence of a hydroid, Eutima japonica, on soft body tissues of age zero Japanese scallop (Mizuhopecten Yessoensis) juveniles cultured in large areas of Funka Bay, Hokkaido. The occurrence coincided with massive death of juvenile scallops. A major objective was to clarify ecological relationships between the symbionts, and to infer the relationship between symbiosis and the massive mortality. To do this, we investigated distributions of association rates of hydroids with juvenile scallops at 15–34 sites over 3 years (2003–2005), with age one adult scallops at 24 sites in 2003, and with mussels at 13 sites in 2004. We studied seasonal changes in association rates with juvenile scallops, and numbers of polyps per juvenile scallop at three sites from November 2003 to June 2004. We also quantified the hydroid impacts on juvenile scallop shell length growth and triglyceride accumulation in the digestive gland. The association rate of E. japonica polyps with juvenile scallops was high in large areas of Funka Bay in 2003, and overlapped the distribution of mussels bearing polyps. Association rates with age one adult scallops were very low in November 2003, even at the sites where polyps were very common on juvenile scallops. Levels of hydroid occurrence in juvenile scallops varies by year. We found that hydroids presence in juvenile scallops declined drastically in 2004 and 2005. The association rates with juvenile scallops, and numbers of polyps per juvenile scallop declined during winter, until they disappeared completely in the following June. Since polyps were rare in adult scallops, we believe that infection of juvenile scallops was probably initiated from the planulae produced by medusae released from polyps growing on Mytilus spp., especially M. galloprovincialis. Subsequently, the inhabitation spread intraspecifically and interspecifically. In juvenile scallops, inhabitation of polyps reduced shell length growth by 43%, and triglyceride accumulation in digestive glands by 24–47%. Inhabitation of E. japonica on juvenile scallop is best regarded as parasitism, rather than inquilinism or commensalism. Occurrence of polyps was probably not a direct lethal factor for juvenile scallops, because there were some sites where association rates were high, but mortalities were low. Massive mortalities in 2003 may have resulted from simultaneous impacts of heavy polyp load and stresses caused by the way in which the animals were handled (transferred from cages for pre-intermediate culture to cages for intermediate culture), because the massive mortality occurred within a month of the transfer. The presence of polyps in juvenile scallops does not affect the quality of the product in Funka Bay, because market size scallops are hydroid-free.

  • relationship between the vitellin concentration in the hemolymph and oocyte necroses during the annual reproduction cycle in the japanese scallop Mizuhopecten Yessoensis
    Invertebrate Reproduction & Development, 2004
    Co-Authors: Katsuhisa Baba, Akira Miyazono, Akihiko Hara
    Abstract:

    Summary A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for measurement of vitellin in the hemolymph and ovary of the Japanese scallop, Mizuhopecten Yessoensis. We observed two peaks of vitellin concentration in the hemolymph during the annual reproductive cycle. The peaks corresponded with the occurrence of two types of oocyte necrosis, type I and type II. Type I necrosis occurs in the early phase of vitellogenesis and is characterized by necrotic oocytes that do not have a germinal vesicle. Type II necrosis occurs during the mid-to late-phase of vitellogenesis and peaks just before spawning. It is characterized by necrotic oocytes whose germinal vesicle is strongly stained by eosin, i.e., the germinal vesicle has lost basophilic properties. In immunohistochemical examinations, oocytes and auxiliary cells (which are in contact with oocytes), reacted with anti-vitellin serum; mid-gut gland, adductor muscles, intestine, gonoduct, kidney, and gill do not. These results sugge...

Zhenmin Bao - One of the best experts on this subject based on the ideXlab platform.

  • systematic identification and validation of the reference genes from 60 rna seq libraries in the scallop Mizuhopecten Yessoensis
    BMC Genomics, 2019
    Co-Authors: Lingling Zhang, Meiwei Zhang, Hao Wang, Shi Wang, Zhenmin Bao
    Abstract:

    Reverse transcription quantitative PCR (RT-qPCR) is widely used for gene expression analysis in various organisms. Its accuracy largely relies on the stability of reference genes, making reference gene selection a vital step in RT-qPCR experiments. However, previous studies in mollusks only focused on the reference genes widely used in vertebrates. In this study, we conducted the transcriptome-wide identification of reference genes in the bivalve mollusk Mizuhopecten Yessoensis based on 60 transcriptomes covering early development, adult tissues and gonadal development. A total of 964, 1210 and 2097 candidate reference genes were identified, respectively, resulting in a core set of 568 genes. Functional enrichment analysis showed that these genes are significantly overrepresented in Gene Ontology (GO) terms or Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to ribosomes, energy production, etc. Six genes (RS23, EF1A, NDUS4, SELR1, EIF3F, and OLA1) were selected from the candidate genes for RT-qPCR validation, together with 6 commonly used reference genes (ACT, CYTC, HEL, EF1B, GAPDH and RPL16). Stability analyses using geNorm, NormFinder and the comparative delta-Ct method revealed that the new candidate reference genes are more stable than the traditionally used genes, and ACT and CYTC are not recommended under either of the three circumstances. There was a significant correlation between the Ct of RT-qPCR and the log2(TPM) of RNA-Seq data (Ct = − 0.94 log2(TPM) + 29.67, R2 = 0.73), making it easy to estimate the Ct values from transcriptome data prior to RT-qPCR experiments. Our study represents the first transcriptome-wide identification of reference genes for early development, adult tissues, and gonadal development in the Yesso scallop and will benefit gene expression studies in other bivalve mollusks.

  • Systematic identification and validation of the reference genes from 60 RNA-Seq libraries in the scallop Mizuhopecten Yessoensis
    'Springer Science and Business Media LLC', 2019
    Co-Authors: Lingling Zhang, Meiwei Zhang, Hao Wang, Shi Wang, Zhenmin Bao
    Abstract:

    Abstract Background Reverse transcription quantitative PCR (RT-qPCR) is widely used for gene expression analysis in various organisms. Its accuracy largely relies on the stability of reference genes, making reference gene selection a vital step in RT-qPCR experiments. However, previous studies in mollusks only focused on the reference genes widely used in vertebrates. Results In this study, we conducted the transcriptome-wide identification of reference genes in the bivalve mollusk Mizuhopecten Yessoensis based on 60 transcriptomes covering early development, adult tissues and gonadal development. A total of 964, 1210 and 2097 candidate reference genes were identified, respectively, resulting in a core set of 568 genes. Functional enrichment analysis showed that these genes are significantly overrepresented in Gene Ontology (GO) terms or Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways related to ribosomes, energy production, etc. Six genes (RS23, EF1A, NDUS4, SELR1, EIF3F, and OLA1) were selected from the candidate genes for RT-qPCR validation, together with 6 commonly used reference genes (ACT, CYTC, HEL, EF1B, GAPDH and RPL16). Stability analyses using geNorm, NormFinder and the comparative delta-Ct method revealed that the new candidate reference genes are more stable than the traditionally used genes, and ACT and CYTC are not recommended under either of the three circumstances. There was a significant correlation between the Ct of RT-qPCR and the log2(TPM) of RNA-Seq data (Ct = − 0.94 log2(TPM) + 29.67, R2 = 0.73), making it easy to estimate the Ct values from transcriptome data prior to RT-qPCR experiments. Conclusion Our study represents the first transcriptome-wide identification of reference genes for early development, adult tissues, and gonadal development in the Yesso scallop and will benefit gene expression studies in other bivalve mollusks

  • characterization of novel microsatellite markers from the yesso scallop Mizuhopecten Yessoensis
    Molecular Ecology Notes, 2006
    Co-Authors: Changsen Sun, Aibin Zhan, Min Hui, Zhenmin Bao
    Abstract:

    AbstractTwelve polymorphic and informative microsatellite markers were isolated and characterizedfor the Yesso scallop, Mizuhopecten Yessoensis . We characterized these loci by genotyping48 individuals; the number of alleles ranged from two to eight, and the values of observedheterozygosity and expected heterozygosity varied from 0 to 0.8333 and from 0.2546 to0.8231, respectively. No significant linkage disequilibrium between pairs of loci was foundand three loci showed significant heterozygote deficiency from the Hardy–Weinberg equi-librium. These markers are potential for studies of the population structure, individual orhybrid identification of the species. Keywords : microsatellites, Mizuhopecten Yessoensis , polymorphic marker, Yesso scallop Received 25 June 2006; revision accepted 25 July 2006 The Yesso scallop, Mizuhopecten Yessoensis (Jay, 1956), isa cold-water scallop species and distributes in northernJapan, Sakhalin, Kuril Islands and northern Korea (Sakurai& Seto 2000; Sato & Nagashima 2001). Having great fameof luxuriant, delicious and large adductor muscle, theYesso scallop is highly required in the Northern American,European and Asian markets; e.g. more than 400 000 tonsper year are produced in Japan (Sato & Nagashima 2001).The Yesso scallop became one of the main bivalves culturedafter being introduced to China in the 1980s. Many breed-ing programs have been carried out to increase the yieldsince 1998. A fundamental question raised is whether thepopulation genetic structures have varied with the wideexpansion of culturing operations of the Yesso scallop. AsDNA markers, microsatellites can be used for the analysisof population structure (Sato & Nagashima 2001; An