The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Albert J R Heck - One of the best experts on this subject based on the ideXlab platform.
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the diverse and expanding role of mass spectrometry in structural and Molecular Biology
The EMBO Journal, 2016Co-Authors: Philip Lossl, Michiel Van De Waterbeemd, Albert J R HeckAbstract:Abstract The emergence of proteomics has led to major technological advances in mass spectrometry (MS). These advancements not only benefitted MS‐based high‐throughput proteomics but also increased the impact of mass spectrometry on the field of structural and Molecular Biology. Here, we review how state‐of‐the‐art MS methods, including native MS, top‐down protein sequencing, cross‐linking‐MS, and hydrogen–deuterium exchange‐MS, nowadays enable the characterization of bioMolecular structures, functions, and interactions. In particular, we focus on the role of mass spectrometry in integrated structural and Molecular Biology investigations of biological macroMolecular complexes and cellular machineries, highlighting work on CRISPR–Cas systems and eukaryotic transcription complexes.
Katherine K Matthay - One of the best experts on this subject based on the ideXlab platform.
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Molecular Biology of neuroblastoma
Journal of Clinical Oncology, 1999Co-Authors: John M Maris, Katherine K MatthayAbstract:PURPOSE AND RESULTS: Neuroblastoma, the most common solid extracranial neoplasm in children, is remarkable for its clinical heterogeneity. Complex patterns of genetic abnormalities interact to determine the clinical phenotype. The Molecular Biology of neuroblastoma is characterized by somatically acquired genetic events that lead to gene overexpression (oncogenes), gene inactivation (tumor suppressor genes), or alterations in gene expression. Amplification of the MYCN proto-oncogene occurs in 20% to 25% of neuroblastomas and is a reliable marker of aggressive clinical behavior. No other oncogene has been shown to be consistently mutated or overexpressed in neuroblastoma, although unbalanced translocations resulting in gain of genetic material from chromosome bands 17q23-qter have been identified in more than 50% of primary tumors. Some children have an inherited predisposition to develop neuroblastoma, but a familial neuroblastoma susceptibility gene has not yet been localized. Consistent areas of chromos...
Norman C Coleman - One of the best experts on this subject based on the ideXlab platform.
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of what use is Molecular Biology to the practicing radiation oncologist
Radiotherapy and Oncology, 1998Co-Authors: Norman C ColemanAbstract:Abstract Background and purpose : We are in a period of rapid advance in understanding the basic mechanisms behind the induction and progression of cancer. The relevance of this new knowledge to the daily clinical practice of radiation oncology may not necessarily be readily apparent. Familiarity with a few of the concepts of Molecular Biology and biochemistry are necessary to fully appreciate the clinical relevance of the new Biology. Methods and results : To illustrate how the new knowledge affects the practice of radiation oncology, examples of the use of Molecular Biology are presented for different clinical aspects of clinical oncology, i.e. screening and prevention, prognostic factors, predictive factors, treatment decision, novel therapy and follow-up. A number of the Molecular Biology techniques are illustrated. Conclusions : The advances from Molecular Biology directly impact the role of radiation oncologists in the clinic. While major new therapies are still in development in the laboratory, these will likely have a very significant role in patient care and cancer prevention in the not-too-distant future. Given the central role of radiation oncologists in cancer management, a basic knowledge of Molecular Biology techniques and their application is essential so that we can be current with our colleagues and patients and as a specialty, participate actively in improving the outcome of patients with or at risk of developing cancer.
Philip Lossl - One of the best experts on this subject based on the ideXlab platform.
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the diverse and expanding role of mass spectrometry in structural and Molecular Biology
The EMBO Journal, 2016Co-Authors: Philip Lossl, Michiel Van De Waterbeemd, Albert J R HeckAbstract:Abstract The emergence of proteomics has led to major technological advances in mass spectrometry (MS). These advancements not only benefitted MS‐based high‐throughput proteomics but also increased the impact of mass spectrometry on the field of structural and Molecular Biology. Here, we review how state‐of‐the‐art MS methods, including native MS, top‐down protein sequencing, cross‐linking‐MS, and hydrogen–deuterium exchange‐MS, nowadays enable the characterization of bioMolecular structures, functions, and interactions. In particular, we focus on the role of mass spectrometry in integrated structural and Molecular Biology investigations of biological macroMolecular complexes and cellular machineries, highlighting work on CRISPR–Cas systems and eukaryotic transcription complexes.
Jizhen Lin - One of the best experts on this subject based on the ideXlab platform.
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panel 4 recent advances in otitis media in Molecular Biology biochemistry genetics and animal models
Otolaryngology-Head and Neck Surgery, 2013Co-Authors: Ann Hermansson, Allen F Ryan, Lauren O Bakaletz, Steve D M Brown, Michael Cheeseman, Steven K Juhn, Timothy T K Jung, David J Lim, Jae Hyang Lim, Jizhen LinAbstract:Background. Otitis media (OM) is the most common childhood bacterial infection and also the leading cause of conductive hearing loss in children. Currently, there is an urgent need for developing novel therapeutic agents for treating OM based on full understanding of Molecular pathogenesis in the areas of Molecular Biology, biochemistry, genetics, and animal model studies in OM. Objective. To provide a state-of-the-art review concerning recent advances in OM in the areas of Molecular Biology, biochemistry, genetics, and animal model studies and to discuss the future directions of OM studies in these areas. Data Sources and Review Methods. A structured search of the current literature (since June 2007). The authors searched PubMed for published literature in the areas of Molecular Biology, biochemistry, genetics, and animal model studies in OM. Results. Over the past 4 years, significant progress has been made in the areas of Molecular Biology, biochemistry, genetics, and animal model studies in OM. These studies brought new insights into our understanding of the Molecular and biochemical mechanisms underlying the Molecular pathogenesis of OM and helped identify novel therapeutic targets for OM. Conclusions and Implications for Practice. Our understanding of the Molecular pathogenesis of OM has been significantly advanced, particularly in the areas of inflammation, innate immunity, mucus overproduction, mucosal hyperplasia, middle ear and inner ear interaction, genetics, genome sequencing, and animal model studies. Although these studies are still in their experimental stages, they help identify new potential therapeutic targets. Future preclinical and clinical studies will help to translate these exciting experimental research findings into clinical applications. (Less)
