The Experts below are selected from a list of 300 Experts worldwide ranked by ideXlab platform
Jiang Chang - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome profile analysis of cell proliferation Molecular Processes during multicellular trichome formation induced by tomato Wo v gene in tobacco
BMC genomics, 2015Co-Authors: Changxian Yang, Yanna Gao, Shenghua Gao, Cheng Xiong, Jiang ChangAbstract:Background Trichomes, developing from the epidermis of nearly all terrestrial plants, provide good structural resistance against insect herbivores and an excellent model for studying the Molecular mechanisms underlying cell fate determination. Regulation of trichomes in Rosids has been well characterized. However, little is known about the cell proliferation Molecular Processes during multicellular trichome formation in Asterids.
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Transcriptome profile analysis of cell proliferation Molecular Processes during multicellular trichome formation induced by tomato Wo (v) gene in tobacco.
Genomics data, 2015Co-Authors: Changxian Yang, Yanna Gao, Shenghua Gao, Cheng Xiong, Jiang ChangAbstract:Trichomes, developing from the epidermis of nearly all terrestrial plants, provide good protection from environmental stress. Regulation of trichomes in Rosids has been well characterized. However, little is known about the cell proliferation Molecular Processes during multicellular trichome formation in Asterids. Ectopic expression of Wo (v) in tobacco and potato induces much more trichome formation than wild type. To gain new insights into the underlying mechanisms during the Processes of these trichomes formation, RNA-seq was employed for the young primary leaf tissues in Wo (v) transgenic and wild-type tobacco. We identified differentially expressed genes which are related to various biological Processes and Molecular functions. Here, we provide details of experimental methods, RNA-seq data (available at Gene Expression Omnibus database under GSE72310). Our data provide new insight into the Molecular Processes controlling multicellular formation in tobacco.
Changxian Yang - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome profile analysis of cell proliferation Molecular Processes during multicellular trichome formation induced by tomato Wo v gene in tobacco
BMC genomics, 2015Co-Authors: Changxian Yang, Yanna Gao, Shenghua Gao, Cheng Xiong, Jiang ChangAbstract:Background Trichomes, developing from the epidermis of nearly all terrestrial plants, provide good structural resistance against insect herbivores and an excellent model for studying the Molecular mechanisms underlying cell fate determination. Regulation of trichomes in Rosids has been well characterized. However, little is known about the cell proliferation Molecular Processes during multicellular trichome formation in Asterids.
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Transcriptome profile analysis of cell proliferation Molecular Processes during multicellular trichome formation induced by tomato Wo (v) gene in tobacco.
Genomics data, 2015Co-Authors: Changxian Yang, Yanna Gao, Shenghua Gao, Cheng Xiong, Jiang ChangAbstract:Trichomes, developing from the epidermis of nearly all terrestrial plants, provide good protection from environmental stress. Regulation of trichomes in Rosids has been well characterized. However, little is known about the cell proliferation Molecular Processes during multicellular trichome formation in Asterids. Ectopic expression of Wo (v) in tobacco and potato induces much more trichome formation than wild type. To gain new insights into the underlying mechanisms during the Processes of these trichomes formation, RNA-seq was employed for the young primary leaf tissues in Wo (v) transgenic and wild-type tobacco. We identified differentially expressed genes which are related to various biological Processes and Molecular functions. Here, we provide details of experimental methods, RNA-seq data (available at Gene Expression Omnibus database under GSE72310). Our data provide new insight into the Molecular Processes controlling multicellular formation in tobacco.
Manuel Manchado - One of the best experts on this subject based on the ideXlab platform.
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Data_Sheet_1_Characterization of Iodine-Related Molecular Processes in the Marine Microalga Tisochrysis lutea (Haptophyta).DOCX
2018Co-Authors: Laura Hernández Javier, Hicham Benzekri, Marta Gut, Gonzalo M. Claros, Stefanie Van Bergeijk, José Pedro Cañavate, Manuel ManchadoAbstract:Iodine metabolism is essential for the antioxidant defense of marine algae and in the biogeochemical cycle of iodine. Moreover, some microalgae can synthetize thyroid hormone-like compounds that are essential to sustain food webs. However, knowledge regarding iodine-related Molecular Processes in microalgae is still scarce. In this study, a de novo transcriptome of Tisochrysis lutea cultured under high iodide concentrations (5 mM) was assembled using both long and short reads. A database termed IsochrysisDB was established to host all genomic information. Gene expression analyses during microalgal growth showed that most of the antioxidant- (aryl, ccp, perox, sod1, sod2, sod3, apx3, ahp1) and iodide-specific deiodinase (dio) genes increased their mRNA abundance progressively until the stationary phase to cope with oxidative stress. Moreover, the increase of dio mRNA abundance in aging cultures indicated that this enzyme was also involved in senescence. Cell treatments with iodide modified the expression of perox whereas treatments with iodate changed the transcript levels of gpx1 and ccp. To test the dependence of perox on iodide, microalgae cells were treated with hydrogen peroxide (H2O2) either in presence or absence of iodide observing that several genes related to reactive oxygen species (ROS) deactivation (perox, gpx1, apx2, apx3, ahp1, ahp2, sod1, sod3, and aryl) were transcriptionally activated although with some temporal differences. However, only the expression of perox was dependent on iodide levels indicating this enzyme, acquired by horizontal gene transfer (HGT), could act as a haloperoxidase. All these data indicate that T. lutea activates coordinately the expression of antioxidant genes to cope with oxidative stress. The identification of a phase-regulated deiodinase and a novel haloperoxidase provide new clues about the origin and evolution of thyroid signaling and the antioxidant role of iodine in the marine environment.
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Image_3_Characterization of Iodine-Related Molecular Processes in the Marine Microalga Tisochrysis lutea (Haptophyta).PDF
2018Co-Authors: Laura Hernández Javier, Hicham Benzekri, Marta Gut, Gonzalo M. Claros, Stefanie Van Bergeijk, José Pedro Cañavate, Manuel ManchadoAbstract:Iodine metabolism is essential for the antioxidant defense of marine algae and in the biogeochemical cycle of iodine. Moreover, some microalgae can synthetize thyroid hormone-like compounds that are essential to sustain food webs. However, knowledge regarding iodine-related Molecular Processes in microalgae is still scarce. In this study, a de novo transcriptome of Tisochrysis lutea cultured under high iodide concentrations (5 mM) was assembled using both long and short reads. A database termed IsochrysisDB was established to host all genomic information. Gene expression analyses during microalgal growth showed that most of the antioxidant- (aryl, ccp, perox, sod1, sod2, sod3, apx3, ahp1) and iodide-specific deiodinase (dio) genes increased their mRNA abundance progressively until the stationary phase to cope with oxidative stress. Moreover, the increase of dio mRNA abundance in aging cultures indicated that this enzyme was also involved in senescence. Cell treatments with iodide modified the expression of perox whereas treatments with iodate changed the transcript levels of gpx1 and ccp. To test the dependence of perox on iodide, microalgae cells were treated with hydrogen peroxide (H2O2) either in presence or absence of iodide observing that several genes related to reactive oxygen species (ROS) deactivation (perox, gpx1, apx2, apx3, ahp1, ahp2, sod1, sod3, and aryl) were transcriptionally activated although with some temporal differences. However, only the expression of perox was dependent on iodide levels indicating this enzyme, acquired by horizontal gene transfer (HGT), could act as a haloperoxidase. All these data indicate that T. lutea activates coordinately the expression of antioxidant genes to cope with oxidative stress. The identification of a phase-regulated deiodinase and a novel haloperoxidase provide new clues about the origin and evolution of thyroid signaling and the antioxidant role of iodine in the marine environment.
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Table_1_Characterization of Iodine-Related Molecular Processes in the Marine Microalga Tisochrysis lutea (Haptophyta).pdf
2018Co-Authors: Laura Hernández Javier, Hicham Benzekri, Marta Gut, Gonzalo M. Claros, Stefanie Van Bergeijk, José Pedro Cañavate, Manuel ManchadoAbstract:Iodine metabolism is essential for the antioxidant defense of marine algae and in the biogeochemical cycle of iodine. Moreover, some microalgae can synthetize thyroid hormone-like compounds that are essential to sustain food webs. However, knowledge regarding iodine-related Molecular Processes in microalgae is still scarce. In this study, a de novo transcriptome of Tisochrysis lutea cultured under high iodide concentrations (5 mM) was assembled using both long and short reads. A database termed IsochrysisDB was established to host all genomic information. Gene expression analyses during microalgal growth showed that most of the antioxidant- (aryl, ccp, perox, sod1, sod2, sod3, apx3, ahp1) and iodide-specific deiodinase (dio) genes increased their mRNA abundance progressively until the stationary phase to cope with oxidative stress. Moreover, the increase of dio mRNA abundance in aging cultures indicated that this enzyme was also involved in senescence. Cell treatments with iodide modified the expression of perox whereas treatments with iodate changed the transcript levels of gpx1 and ccp. To test the dependence of perox on iodide, microalgae cells were treated with hydrogen peroxide (H2O2) either in presence or absence of iodide observing that several genes related to reactive oxygen species (ROS) deactivation (perox, gpx1, apx2, apx3, ahp1, ahp2, sod1, sod3, and aryl) were transcriptionally activated although with some temporal differences. However, only the expression of perox was dependent on iodide levels indicating this enzyme, acquired by horizontal gene transfer (HGT), could act as a haloperoxidase. All these data indicate that T. lutea activates coordinately the expression of antioxidant genes to cope with oxidative stress. The identification of a phase-regulated deiodinase and a novel haloperoxidase provide new clues about the origin and evolution of thyroid signaling and the antioxidant role of iodine in the marine environment.
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Data_Sheet_3_Characterization of Iodine-Related Molecular Processes in the Marine Microalga Tisochrysis lutea (Haptophyta).XLSX
2018Co-Authors: Laura Hernández Javier, Hicham Benzekri, Marta Gut, Gonzalo M. Claros, Stefanie Van Bergeijk, José Pedro Cañavate, Manuel ManchadoAbstract:Iodine metabolism is essential for the antioxidant defense of marine algae and in the biogeochemical cycle of iodine. Moreover, some microalgae can synthetize thyroid hormone-like compounds that are essential to sustain food webs. However, knowledge regarding iodine-related Molecular Processes in microalgae is still scarce. In this study, a de novo transcriptome of Tisochrysis lutea cultured under high iodide concentrations (5 mM) was assembled using both long and short reads. A database termed IsochrysisDB was established to host all genomic information. Gene expression analyses during microalgal growth showed that most of the antioxidant- (aryl, ccp, perox, sod1, sod2, sod3, apx3, ahp1) and iodide-specific deiodinase (dio) genes increased their mRNA abundance progressively until the stationary phase to cope with oxidative stress. Moreover, the increase of dio mRNA abundance in aging cultures indicated that this enzyme was also involved in senescence. Cell treatments with iodide modified the expression of perox whereas treatments with iodate changed the transcript levels of gpx1 and ccp. To test the dependence of perox on iodide, microalgae cells were treated with hydrogen peroxide (H2O2) either in presence or absence of iodide observing that several genes related to reactive oxygen species (ROS) deactivation (perox, gpx1, apx2, apx3, ahp1, ahp2, sod1, sod3, and aryl) were transcriptionally activated although with some temporal differences. However, only the expression of perox was dependent on iodide levels indicating this enzyme, acquired by horizontal gene transfer (HGT), could act as a haloperoxidase. All these data indicate that T. lutea activates coordinately the expression of antioxidant genes to cope with oxidative stress. The identification of a phase-regulated deiodinase and a novel haloperoxidase provide new clues about the origin and evolution of thyroid signaling and the antioxidant role of iodine in the marine environment.
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Table_2_Characterization of Iodine-Related Molecular Processes in the Marine Microalga Tisochrysis lutea (Haptophyta).docx
2018Co-Authors: Laura Hernández Javier, Hicham Benzekri, Marta Gut, Gonzalo M. Claros, Stefanie Van Bergeijk, José Pedro Cañavate, Manuel ManchadoAbstract:Iodine metabolism is essential for the antioxidant defense of marine algae and in the biogeochemical cycle of iodine. Moreover, some microalgae can synthetize thyroid hormone-like compounds that are essential to sustain food webs. However, knowledge regarding iodine-related Molecular Processes in microalgae is still scarce. In this study, a de novo transcriptome of Tisochrysis lutea cultured under high iodide concentrations (5 mM) was assembled using both long and short reads. A database termed IsochrysisDB was established to host all genomic information. Gene expression analyses during microalgal growth showed that most of the antioxidant- (aryl, ccp, perox, sod1, sod2, sod3, apx3, ahp1) and iodide-specific deiodinase (dio) genes increased their mRNA abundance progressively until the stationary phase to cope with oxidative stress. Moreover, the increase of dio mRNA abundance in aging cultures indicated that this enzyme was also involved in senescence. Cell treatments with iodide modified the expression of perox whereas treatments with iodate changed the transcript levels of gpx1 and ccp. To test the dependence of perox on iodide, microalgae cells were treated with hydrogen peroxide (H2O2) either in presence or absence of iodide observing that several genes related to reactive oxygen species (ROS) deactivation (perox, gpx1, apx2, apx3, ahp1, ahp2, sod1, sod3, and aryl) were transcriptionally activated although with some temporal differences. However, only the expression of perox was dependent on iodide levels indicating this enzyme, acquired by horizontal gene transfer (HGT), could act as a haloperoxidase. All these data indicate that T. lutea activates coordinately the expression of antioxidant genes to cope with oxidative stress. The identification of a phase-regulated deiodinase and a novel haloperoxidase provide new clues about the origin and evolution of thyroid signaling and the antioxidant role of iodine in the marine environment.
Cheng Xiong - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome profile analysis of cell proliferation Molecular Processes during multicellular trichome formation induced by tomato Wo v gene in tobacco
BMC genomics, 2015Co-Authors: Changxian Yang, Yanna Gao, Shenghua Gao, Cheng Xiong, Jiang ChangAbstract:Background Trichomes, developing from the epidermis of nearly all terrestrial plants, provide good structural resistance against insect herbivores and an excellent model for studying the Molecular mechanisms underlying cell fate determination. Regulation of trichomes in Rosids has been well characterized. However, little is known about the cell proliferation Molecular Processes during multicellular trichome formation in Asterids.
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Transcriptome profile analysis of cell proliferation Molecular Processes during multicellular trichome formation induced by tomato Wo (v) gene in tobacco.
Genomics data, 2015Co-Authors: Changxian Yang, Yanna Gao, Shenghua Gao, Cheng Xiong, Jiang ChangAbstract:Trichomes, developing from the epidermis of nearly all terrestrial plants, provide good protection from environmental stress. Regulation of trichomes in Rosids has been well characterized. However, little is known about the cell proliferation Molecular Processes during multicellular trichome formation in Asterids. Ectopic expression of Wo (v) in tobacco and potato induces much more trichome formation than wild type. To gain new insights into the underlying mechanisms during the Processes of these trichomes formation, RNA-seq was employed for the young primary leaf tissues in Wo (v) transgenic and wild-type tobacco. We identified differentially expressed genes which are related to various biological Processes and Molecular functions. Here, we provide details of experimental methods, RNA-seq data (available at Gene Expression Omnibus database under GSE72310). Our data provide new insight into the Molecular Processes controlling multicellular formation in tobacco.
Shenghua Gao - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome profile analysis of cell proliferation Molecular Processes during multicellular trichome formation induced by tomato Wo v gene in tobacco
BMC genomics, 2015Co-Authors: Changxian Yang, Yanna Gao, Shenghua Gao, Cheng Xiong, Jiang ChangAbstract:Background Trichomes, developing from the epidermis of nearly all terrestrial plants, provide good structural resistance against insect herbivores and an excellent model for studying the Molecular mechanisms underlying cell fate determination. Regulation of trichomes in Rosids has been well characterized. However, little is known about the cell proliferation Molecular Processes during multicellular trichome formation in Asterids.
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Transcriptome profile analysis of cell proliferation Molecular Processes during multicellular trichome formation induced by tomato Wo (v) gene in tobacco.
Genomics data, 2015Co-Authors: Changxian Yang, Yanna Gao, Shenghua Gao, Cheng Xiong, Jiang ChangAbstract:Trichomes, developing from the epidermis of nearly all terrestrial plants, provide good protection from environmental stress. Regulation of trichomes in Rosids has been well characterized. However, little is known about the cell proliferation Molecular Processes during multicellular trichome formation in Asterids. Ectopic expression of Wo (v) in tobacco and potato induces much more trichome formation than wild type. To gain new insights into the underlying mechanisms during the Processes of these trichomes formation, RNA-seq was employed for the young primary leaf tissues in Wo (v) transgenic and wild-type tobacco. We identified differentially expressed genes which are related to various biological Processes and Molecular functions. Here, we provide details of experimental methods, RNA-seq data (available at Gene Expression Omnibus database under GSE72310). Our data provide new insight into the Molecular Processes controlling multicellular formation in tobacco.