The Experts below are selected from a list of 168 Experts worldwide ranked by ideXlab platform
Toru Fujiwara - One of the best experts on this subject based on the ideXlab platform.
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effects of Molybdenum Deficiency and defects in molybdate transporter mot1 on transcript accumulation and nitrogen sulphur metabolism in arabidopsis thaliana
Journal of Experimental Botany, 2011Co-Authors: Miyako Kusano, Akira Oikawa, Atsushi Fukushima, Hajime Tomatsu, Kazuki Saito, Masami Yokota Hirai, Toru FujiwaraAbstract:Molybdenum (Mo) is a micronutrient essential for plant growth, as several key enzymes of plant metabolic pathways contain Mo cofactor in their catalytic centres. Mo-containing oxidoreductases include nitrate reductase, sulphite oxidase, xanthine dehydrogenase, and aldehyde oxidase. These are involved in nitrate assimilation, sulphite detoxification, purine metabolism or the synthesis of abscisic acid, auxin and glucosinolates in plants. To understand the effects of Mo Deficiency and a mutation in a molybdate transporter, MOT1, on nitrogen and sulphur metabolism in Arabidopsis thaliana, transcript and metabolite profiling of the mutant lacking MOT1 was conducted in the presence or absence of Mo. Transcriptome analysis revealed that Mo Deficiency had impacts on genes involved in metabolisms, transport, stress responses, and signal transductions. The transcript level of a nitrate reductase NR1 was highly induced under Mo Deficiency in mot1-1. The metabolite profiles were analysed further by using gas chromatography time-of-flight mass spectrometry, capillary electrophoresis time-of-flight mass spectrometry, and ultra high performance liquid chromatography. The levels of amino acids, sugars, organic acids, and purine metabolites were altered significantly in the Mo-deficient plants. These results are the first investigation of the global effect of Mo nutrition and MOT1 on plant gene expressions and metabolism.
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Effects of Molybdenum Deficiency and defects in molybdate transporter MOT1 on transcript accumulation and nitrogen/sulphur metabolism in Arabidopsis thaliana
Journal of Experimental Botany, 2010Co-Authors: Miyako Kusano, Akira Oikawa, Atsushi Fukushima, Hajime Tomatsu, Kazuki Saito, Masami Yokota Hirai, Toru FujiwaraAbstract:Molybdenum (Mo) is a micronutrient essential for plant growth, as several key enzymes of plant metabolic pathways contain Mo cofactor in their catalytic centres. Mo-containing oxidoreductases include nitrate reductase, sulphite oxidase, xanthine dehydrogenase, and aldehyde oxidase. These are involved in nitrate assimilation, sulphite detoxification, purine metabolism or the synthesis of abscisic acid, auxin and glucosinolates in plants. To understand the effects of Mo Deficiency and a mutation in a molybdate transporter, MOT1, on nitrogen and sulphur metabolism in Arabidopsis thaliana, transcript and metabolite profiling of the mutant lacking MOT1 was conducted in the presence or absence of Mo. Transcriptome analysis revealed that Mo Deficiency had impacts on genes involved in metabolisms, transport, stress responses, and signal transductions. The transcript level of a nitrate reductase NR1 was highly induced under Mo Deficiency in mot1-1. The metabolite profiles were analysed further by using gas chromatography time-of-flight mass spectrometry, capillary electrophoresis time-of-flight mass spectrometry, and ultra high performance liquid chromatography. The levels of amino acids, sugars, organic acids, and purine metabolites were altered significantly in the Mo-deficient plants. These results are the first investigation of the global effect of Mo nutrition and MOT1 on plant gene expressions and metabolism.
J. H. Kritzinger - One of the best experts on this subject based on the ideXlab platform.
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The effect of Molybdenum on seed dormancy in wheat
Plant and Soil, 1992Co-Authors: A. L. P. Cairns, J. H. KritzingerAbstract:Molybdenum Deficiency was induced in wheat growing in sand culture under controlled environmental conditions. Half the plants were treated with foliar applications of Molybdenum at the six and flag leaf stages.
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The effect of Molybdenum on seed dormancy in wheat
Plant and Soil, 1992Co-Authors: A. L. P. Cairns, J. H. KritzingerAbstract:Molybdenum Deficiency was induced in wheat growing in sand culture under controlled environmental conditions. Half the plants were treated with foliar applications of Molybdenum at the six and flag leaf stages. Molybdenum-treated plants produced seed which was significantly more dormant that that harvested from the Molybdenum-deficient plants. Molybdenum treatment also resulted in a higher nitrate and protein content of the seed. These findings, seen in the light of the proven success of Molybdenum in preventing pre-harvest sprouting in maize, would seem to indicate that Molybdenum has the potential to restrict pre-harvest sprouting losses in wheat growing in soils deficient in Molybdenum.
A. L. P. Cairns - One of the best experts on this subject based on the ideXlab platform.
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Molybdenum Deficiency in wheat results in lower dormancy levels via reduced aba
Seed Science Research, 1994Co-Authors: A T Modi, A. L. P. CairnsAbstract:Wheat which was grown in acid-washed sand and irrigated with a Molybdenum-free nutrient solution was treated with various concentrations of Molybdenum (Mo) as a foliar spray at the flag leaf stage. At maturity, dormancy levels and abscisic acid (ABA) content of the seed were determined. Seed dormancy and ABA content increased with increasing rates of Mo application. In a field experiment, wheat (cv. SST 66) was treated with 100 ppm Mo by foliar application at the flag leaf stage. Embryos were tested for sensitivity to exogenously applied ABA. Embryos from Mo-treated plants showed enhanced sensitivity to ABA-induced inhibition of germination. The Mo application also resulted in significantly higher levels of endogenous ABA and Mo in the seeds. It is postulated that Mo Deficiency leads to a lack of dormancy in wheat via reduced synthesis of ABA.
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The effect of Molybdenum on seed dormancy in wheat
Plant and Soil, 1992Co-Authors: A. L. P. Cairns, J. H. KritzingerAbstract:Molybdenum Deficiency was induced in wheat growing in sand culture under controlled environmental conditions. Half the plants were treated with foliar applications of Molybdenum at the six and flag leaf stages.
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The effect of Molybdenum on seed dormancy in wheat
Plant and Soil, 1992Co-Authors: A. L. P. Cairns, J. H. KritzingerAbstract:Molybdenum Deficiency was induced in wheat growing in sand culture under controlled environmental conditions. Half the plants were treated with foliar applications of Molybdenum at the six and flag leaf stages. Molybdenum-treated plants produced seed which was significantly more dormant that that harvested from the Molybdenum-deficient plants. Molybdenum treatment also resulted in a higher nitrate and protein content of the seed. These findings, seen in the light of the proven success of Molybdenum in preventing pre-harvest sprouting in maize, would seem to indicate that Molybdenum has the potential to restrict pre-harvest sprouting losses in wheat growing in soils deficient in Molybdenum.
Miyako Kusano - One of the best experts on this subject based on the ideXlab platform.
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effects of Molybdenum Deficiency and defects in molybdate transporter mot1 on transcript accumulation and nitrogen sulphur metabolism in arabidopsis thaliana
Journal of Experimental Botany, 2011Co-Authors: Miyako Kusano, Akira Oikawa, Atsushi Fukushima, Hajime Tomatsu, Kazuki Saito, Masami Yokota Hirai, Toru FujiwaraAbstract:Molybdenum (Mo) is a micronutrient essential for plant growth, as several key enzymes of plant metabolic pathways contain Mo cofactor in their catalytic centres. Mo-containing oxidoreductases include nitrate reductase, sulphite oxidase, xanthine dehydrogenase, and aldehyde oxidase. These are involved in nitrate assimilation, sulphite detoxification, purine metabolism or the synthesis of abscisic acid, auxin and glucosinolates in plants. To understand the effects of Mo Deficiency and a mutation in a molybdate transporter, MOT1, on nitrogen and sulphur metabolism in Arabidopsis thaliana, transcript and metabolite profiling of the mutant lacking MOT1 was conducted in the presence or absence of Mo. Transcriptome analysis revealed that Mo Deficiency had impacts on genes involved in metabolisms, transport, stress responses, and signal transductions. The transcript level of a nitrate reductase NR1 was highly induced under Mo Deficiency in mot1-1. The metabolite profiles were analysed further by using gas chromatography time-of-flight mass spectrometry, capillary electrophoresis time-of-flight mass spectrometry, and ultra high performance liquid chromatography. The levels of amino acids, sugars, organic acids, and purine metabolites were altered significantly in the Mo-deficient plants. These results are the first investigation of the global effect of Mo nutrition and MOT1 on plant gene expressions and metabolism.
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Effects of Molybdenum Deficiency and defects in molybdate transporter MOT1 on transcript accumulation and nitrogen/sulphur metabolism in Arabidopsis thaliana
Journal of Experimental Botany, 2010Co-Authors: Miyako Kusano, Akira Oikawa, Atsushi Fukushima, Hajime Tomatsu, Kazuki Saito, Masami Yokota Hirai, Toru FujiwaraAbstract:Molybdenum (Mo) is a micronutrient essential for plant growth, as several key enzymes of plant metabolic pathways contain Mo cofactor in their catalytic centres. Mo-containing oxidoreductases include nitrate reductase, sulphite oxidase, xanthine dehydrogenase, and aldehyde oxidase. These are involved in nitrate assimilation, sulphite detoxification, purine metabolism or the synthesis of abscisic acid, auxin and glucosinolates in plants. To understand the effects of Mo Deficiency and a mutation in a molybdate transporter, MOT1, on nitrogen and sulphur metabolism in Arabidopsis thaliana, transcript and metabolite profiling of the mutant lacking MOT1 was conducted in the presence or absence of Mo. Transcriptome analysis revealed that Mo Deficiency had impacts on genes involved in metabolisms, transport, stress responses, and signal transductions. The transcript level of a nitrate reductase NR1 was highly induced under Mo Deficiency in mot1-1. The metabolite profiles were analysed further by using gas chromatography time-of-flight mass spectrometry, capillary electrophoresis time-of-flight mass spectrometry, and ultra high performance liquid chromatography. The levels of amino acids, sugars, organic acids, and purine metabolites were altered significantly in the Mo-deficient plants. These results are the first investigation of the global effect of Mo nutrition and MOT1 on plant gene expressions and metabolism.
A I Netrusov - One of the best experts on this subject based on the ideXlab platform.
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Alternative NAD(+)-dependent formate dehydrogenases in the facultative methylotroph Mycobacterium vaccae 10.
FEMS microbiology letters, 1991Co-Authors: V V Karzanov, C M Correa, Y G Bogatsky, A I NetrusovAbstract:Mycobacterium vaccae 10 growing in methanol medium synthesizes two inducible alternative NAD(+)-dependent formate dehydrogenases (FDH). In the presence of Molybdenum, the dominating form of the enzyme is FDHI with Mr 440 kDa and Km 0.32 mM for sodium formate. FDHI reduced ferricyanide as well as NAD+, and it was reversibly inactivated by formate. NAD+ stabilized FDHI against this inactivation. Under conditions of artificial Molybdenum Deficiency (tungsten in the medium), the second enzyme (FDHII) appeared with Mr about 93 kDa and Km 8.3 mM for sodium formate, and no FDHI activity was detected. FDHII did not reduce ferricyanide and was not inactivated by formate. The activity of FDHI was restored in tungsten-grown cells by pulse addition of Molybdenum under conditions of blocked protein synthesis, suggesting the pre-existence of inactive apo-FDHI.
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Alternative NAD+-dependent formate dehydrogenases in the facultative methylotroph Mycobacterium vaccae 10
Fems Microbiology Letters, 1991Co-Authors: V V Karzanov, C M Correa, Y G Bogatsky, A I NetrusovAbstract:Abstract Mycobacterium vaccae 10 growing in methanol medium synthesizes two inducible alternative NAD + -dependent formate dehydrogenases (FDH). In the presence of Molybdenum, the dominating form of the enzyme is FDHI with M r 440 kDa and K m 0.32 mM for sodium formate. FDHI reduced ferricyanide as well as NAD + , and it was reversibly inactivated by formate. NAD + stabilized FDHI against this inactivation. Under conditions of artificial Molybdenum Deficiency (tungsten in the medium), the second enzyme (FDHII) appeared with M r about 93 kDa and K m 8.3 mM for sodium formate, and no FDHI activity was detected. FDHII did not reduce ferricyanide and was not inactivated by formate. The activity of FDHI was restored in tungsten-grown cells by pulse addition of Molybdenum under conditions of blocked protein synthesis, suggesting the pre-existence of inactive apo-FDHI.