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G. Y. Sy - One of the best experts on this subject based on the ideXlab platform.
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evaluation de l activite antioxydante des feuilles de Moringa oleifera lam Moringaceae du senegal
International Journal of Biological and Chemical Sciences, 2018Co-Authors: Awa Ndiaye Sy, A M Dieye, Alioune Diorfall, Mamadou Ndiaye, Khadim Ndiaye, Rokhaya Sylla Gueye, E Bassene, G. Y. SyAbstract:Les plantes medicinales telles que Moringa oleifera, presentent en general de nombreuses vertus therapeutiques. L’objectif de cette etude etait d’evaluer l’activite antioxydante des extraits des feuilles de Moringa oleifera par la methode de piegeage des radicaux libres 2,2-diphenyl-1- picryl-hydrazyle (DPPH•). Une extraction ethanolique a ete effectuee et l’extrait sec obtenu a ensuite ete fractionne par la methode de separation liquide-liquide avec des solvants polaires (eau distillee et acetate d’ethyle) et apolaires (dichloromethane). L’extrait ethanolique, ses fractions et l’acide L-ascorbique (antioxydant de reference), ont ete testes a differentes concentrations: 12,5; 25; 50; 100 et 200 μg/ml. La lecture de l’absorbance des produits s’est faite au bout de 30 minutes au spectrophotometre a 517 nm. Il ressort de cette etude que l’extrait ethanolique des feuilles de Moringa oleifera et ses differentes fractions possedent une activite antioxydante significative qui est dose-dependante.Cependant, l’activite la plus importante a ete observee avec l’extrait ethanolique avec une concentration inhibitrice 50 (CI50) de 87,86±1,80 μg/ml. Les resultats de cette etude suggerent que les feuilles de Moringa oleifera contiennent des substances a proprietes antioxydantes qui pourraient contribuer a la prevention de certaines maladies chroniques telles que le cancer, les maladies cardiovasculaires entre autres. © 2018 International Formulae Group. All rights reserved. Mots cles: Moringa oleifera , activite antioxydante, fractionnement, DPPH English Title: Evaluation of the antioxidant activity of leaves of Moringa oleifera Lam. (Moringaceae) of Senegal English Abstract Medicinal plants such as Moringa oleifera generally have several therapeutic virtues. This study aimed to evaluate the antioxidant activity of Moringa oleifera leaves extracts using the free radical scavenging method: 2,2-diphenyl-1-picryl-hydrazyl (DPPH•) assay. Ethanolic extraction was carried out and the dry extract obtained was then fractionated by the liquid-liquid separation method with polar solvents (distilled water and ethyl acetate) and non polar solvent (dichloromethane). The antioxidant activities of the ethanolic extract, its fractions and the Lascorbic acid (reference antioxidant) were tested at various concentrations of 12.5; 25; 50; 100 and 200 μg/ml. The absorbance of the products was read after 30 minutes at the spectrophotometer at 517 nm. It emerged from this study that the ethanolic extract of Moringa oleifera leaves and its different fractions had significant antioxidant activity that is dose-dependent. However, the ethanolic extract was more active on DPPH radical inhibition with a concentration of sample required to scavenge 50% of free radicals (CI50) of 87.86 ± 1.80 μg/ml. These results suggest that leaves of Moringa oleifera contain substances with antioxidant properties that could contribute to the prevention of chronic diseases such as cancer, cardiovascular diseases, among others. © 2018 International Formulae Group. All rights reserved. Keywords: Moringa oleifera , antioxidant activity, DPPH
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evaluation de l activite antioxydante des feuilles de Moringa oleifera lam Moringaceae du senegal
International Journal of Biological and Chemical Sciences, 2018Co-Authors: Awa Ndiaye Sy, A M Dieye, Alioune Diorfall, Mamadou Ndiaye, Khadim Ndiaye, Rokhaya Sylla Gueye, E Bassene, G. Y. SyAbstract:Les plantes medicinales telles que Moringa oleifera, presentent en general de nombreuses vertus therapeutiques. L’objectif de cette etude etait d’evaluer l’activite antioxydante des extraits des feuilles de Moringa oleifera par la methode de piegeage des radicaux libres 2,2-diphenyl-1- picryl-hydrazyle (DPPH•). Une extraction ethanolique a ete effectuee et l’extrait sec obtenu a ensuite ete fractionne par la methode de separation liquide-liquide avec des solvants polaires (eau distillee et acetate d’ethyle) et apolaires (dichloromethane). L’extrait ethanolique, ses fractions et l’acide L-ascorbique (antioxydant de reference), ont ete testes a differentes concentrations: 12,5; 25; 50; 100 et 200 μg/ml. La lecture de l’absorbance des produits s’est faite au bout de 30 minutes au spectrophotometre a 517 nm. Il ressort de cette etude que l’extrait ethanolique des feuilles de Moringa oleifera et ses differentes fractions possedent une activite antioxydante significative qui est dose-dependante.Cependant, l’activite la plus importante a ete observee avec l’extrait ethanolique avec une concentration inhibitrice 50 (CI50) de 87,86±1,80 μg/ml. Les resultats de cette etude suggerent que les feuilles de Moringa oleifera contiennent des substances a proprietes antioxydantes qui pourraient contribuer a la prevention de certaines maladies chroniques telles que le cancer, les maladies cardiovasculaires entre autres. © 2018 International Formulae Group. All rights reserved. Mots cles: Moringa oleifera , activite antioxydante, fractionnement, DPPH English Title: Evaluation of the antioxidant activity of leaves of Moringa oleifera Lam. (Moringaceae) of Senegal English Abstract Medicinal plants such as Moringa oleifera generally have several therapeutic virtues. This study aimed to evaluate the antioxidant activity of Moringa oleifera leaves extracts using the free radical scavenging method: 2,2-diphenyl-1-picryl-hydrazyl (DPPH•) assay. Ethanolic extraction was carried out and the dry extract obtained was then fractionated by the liquid-liquid separation method with polar solvents (distilled water and ethyl acetate) and non polar solvent (dichloromethane). The antioxidant activities of the ethanolic extract, its fractions and the Lascorbic acid (reference antioxidant) were tested at various concentrations of 12.5; 25; 50; 100 and 200 μg/ml. The absorbance of the products was read after 30 minutes at the spectrophotometer at 517 nm. It emerged from this study that the ethanolic extract of Moringa oleifera leaves and its different fractions had significant antioxidant activity that is dose-dependent. However, the ethanolic extract was more active on DPPH radical inhibition with a concentration of sample required to scavenge 50% of free radicals (CI50) of 87.86 ± 1.80 μg/ml. These results suggest that leaves of Moringa oleifera contain substances with antioxidant properties that could contribute to the prevention of chronic diseases such as cancer, cardiovascular diseases, among others. © 2018 International Formulae Group. All rights reserved. Keywords: Moringa oleifera , antioxidant activity, DPPH
Nabilah Munifah - One of the best experts on this subject based on the ideXlab platform.
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DAYA INTERAKSI EKSTRAK ETANOL DAUN KELOR (Moringa oleifera) DENGAN PORPHYROMONAS GINGIVALIS ATCC 33277 TERHADAP PRODUKSI SENYAWA VOLATIL SULFUR DAN PH SALIVA
Fakultas Kedokteran Gigi Universitas Mahasaraswati Denpasar, 2021Co-Authors: Nabilah MunifahAbstract:Bakteri yang paling dominan memproduksi gas Volatile Sulfur Compounds (VSCs) penyebab halitosis adalah Porphyromonas gingivalis.Bakteri ini memiliki aktivitas proteolisis yang kuat sehingga dapat mendegradasi protein dengan reaksi enzimatik dan menghasilkan Volatile Sulfur Compounds (VSCs). Saliva dengan pH sedikit asam dapat menekan pertumbuhan dan proliferasi bakteri anaerob Gram negatif sehingga menghambat aktivasi enzim yang dapat menghasilkan sulfur penyebab halitosis. Pada daun kelor (Moringa oleifera) terdapat beberapa senyawa yang memiliki kemampuan menghambat bakteri. Penelitian ini bertujuan untuk mengetahui daya interaksi ekstrak etanol daun kelor (Moringa oleifera) dengan bakteri Porphyromonas gingivalis ATCC 33277 terhadap produksi senyawa volatil sulfur dan pH saliva. Uji interaksi ekstrak etanol daun kelor (Moringa oleifera) dengan produksi senyawa volatil sulfur oleh bakteri Porphyromonas gingivalis ATCC 33277 menggunakan media SIM (Sulfide Indole Motility). Hasil penelitian dianalisis menggunakan uji statistik Independent sample test dan Kruskal-Wallis. Konsentrasi ekstrak etanol daun kelor (Moringa oleifera) yang digunakan adalah 75%, 50%, 25%, 12,5%, 6,25% dengan waktu inkubasi 24 serta 48 jam. Berdasarkan hasil penelitian dapat disimpulkan bahwa terdapat pengaruh daya interaksi ekstrak etanol daun kelor (Moringa oleifera) dengan Porphyromonas gingivalis ATCC 33277 terhadap produksi senyawa volatil sulfur. Ekstrak etanol daun kelor (Moringa oleifera) dapat mempertahankan pH saliva setelah di interaksikan dengan Porphyromonas gingivalis ATCC 33277.Banda Ace
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DAYA INTERAKSI EKSTRAK ETANOL DAUN KELOR (Moringa oleifera) DENGAN PORPHYROMONAS GINGIVALIS ATCC 33277 TERHADAP PRODUKSI SENYAWA VOLATIL SULFUR DAN PH SALIVA
Fakultas Kedokteran Gigi Universitas Mahasaraswati Denpasar, 2021Co-Authors: Nabilah MunifahAbstract:Bakteri yang paling dominan memproduksi gas Volatile Sulfur Compounds (VSCs) penyebab halitosis adalah Porphyromonas gingivalis.Bakteri ini memiliki aktivitas proteolisis yang kuat sehingga dapat mendegradasi protein dengan reaksi enzimatik dan menghasilkan Volatile Sulfur Compounds (VSCs). Saliva dengan pH sedikit asam dapat menekan pertumbuhan dan proliferasi bakteri anaerob Gram negatif sehingga menghambat aktivasi enzim yang dapat menghasilkan sulfur penyebab halitosis. Pada daun kelor (Moringa oleifera) terdapat beberapa senyawa yang memiliki kemampuan menghambat bakteri. Penelitian ini bertujuan untuk mengetahui daya interaksi ekstrak etanol daun kelor (Moringa oleifera) dengan bakteri Porphyromonas gingivalis ATCC 33277 terhadap produksi senyawa volatil sulfur dan pH saliva. Uji interaksi ekstrak etanol daun kelor (Moringa oleifera) dengan produksi senyawa volatil sulfur oleh bakteri Porphyromonas gingivalis ATCC 33277 menggunakan media SIM (Sulfide Indole Motility). Hasil penelitian dianalisis menggunakan uji statistik Independent sample test dan Kruskal-Wallis. Konsentrasi ekstrak etanol daun kelor (Moringa oleifera) yang digunakan adalah 75%, 50%, 25%, 12,5%, 6,25% dengan waktu inkubasi 24 serta 48 jam. Berdasarkan hasil penelitian waktu inkubasi memiliki hubungan positif dalam menghambat produksi senyawa volatil sulfur. Waktu inkubasi dan konsentrasi ekstrak etanol daun kelor (Moringa oleifera) memiliki hubungan positif dalam mempertahankan pH saliva. Pada waktu inkubasi 48 jam hampir seluruh ekstrak etanol daun kelor (75%,50%,25%, dan 12,5%) memiliki kemampuan menurunkan produksi senyawa volatil sulfur sebanyak 50%. Konsentrasi 75% dengan waktu inkubasi 48 jam paling kuat dalam mempertahankan pH saliva.Banda Ace
Poppy Mila Fadriani - One of the best experts on this subject based on the ideXlab platform.
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EFEKTIVITAS PHYTO RESPON EKSTRAK ETANOL DAUN KELOR (Moringa oleifera) TERHADAP VIRULENSI PORPHYROMONAS GINGIVALIS ATCC 33277 SECARA IN-VITRO
Fakultas Kedokteran Gigi Universitas Mahasaraswati Denpasar, 2020Co-Authors: Poppy Mila FadrianiAbstract:Porphyromonas gingivalis ATCC 33277 merupakan bakteri biakan murni yang memiliki virulensi paling rendah dibandingkan dengan biakan murni Porphyromonas gingivalis lainnya. Porphyromonas gingivalis merupakan bakteri Gram-negatif anaerob obligat yang menjadi salah satu bakteri dominan penyebab periodontitis kronis.Terapi periodontitis kronis meliputi menghilangkan bakteri patogen seperti Porphyromonas gingivalis. Penggunaan daun kelor(Moringa oleifera) dapat digunakan sebagai terapi untuk mengeliminasi bakteri patogen karena mengandung senyawa antibakteri. Penelitian ini bertujuan untuk mengetahui efektivitas phyto respon ekstrak etanol daun kelor (Moringa oleifera) terhadap virulensi Porphyromonas gingivalis ATCC 33277. Uji potensi anti virulensi ekstrak etanol daun kelor (Moringa oleifera) terhadap Porphyromonas gingivalis ATCC 33277 dilakukan dengan menggunakan metode difusi (sumuran) sebanyak 2 kali pengulangan pada media Tryptone Soy Agar (TSA). Konsentrasi ekstrak etanol 96% daun kelor (Moringa oleifera) yang digunakan pada penelitian ini yaitu 75%, 50%, 25%, 12,5%, dan 6,25%. Data hasil penelitian dianalisis menggunakan uji one way ANOVA. Berdasarkan hasil penelitian dapat disimpulkan bahwa ekstrak etanol daun kelor (Moringa oleifera) pada konsentrasi 50% dengan waktu inkubasi 24 jam memiliki efektivitas phyto respon paling kuat terhadap virulensi Porphyromonas gingivalis ATCC 33277 secara in-vitro.Banda Ace
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EFEKTIVITAS PHYTO RESPON EKSTRAK ETANOL DAUN KELOR (Moringa oleifera) TERHADAP VIRULENSIPORPHYROMONASGINGIVALIS ATCC 33277 SECARA IN-VITRO
Fakultas Kedokteran Gigi Universitas Mahasaraswati Denpasar, 2020Co-Authors: Poppy Mila FadrianiAbstract:Porphyromonas gingivalis ATCC 33277 merupakan bakteri biakan murni yang memiliki virulensi paling rendah dibandingkan dengan biakan murni Porphyromonas gingivalis lainnya. Porphyromonas gingivalis merupakan bakteri Gram-negatif anaerob obligat yang menjadi salah satu bakteri dominan penyebab periodontitis kronis.Terapi periodontitis kronis meliputi menghilangkan bakteri patogen seperti Porphyromonas gingivalis. Penggunaan daun kelor(Moringa oleifera) dapat digunakan sebagai terapi untuk mengeliminasi bakteri patogen karena mengandung senyawa antibakteri. Penelitian ini bertujuan untuk mengetahui efektivitas phyto respon ekstrak etanol daun kelor (Moringa oleifera) terhadap virulensi Porphyromonas gingivalis ATCC 33277. Uji potensi anti virulensi ekstrak etanol daun kelor (Moringa oleifera) terhadap Porphyromonas gingivalis ATCC 33277 dilakukan dengan menggunakan metode difusi (sumuran) sebanyak 2 kali pengulangan pada media Tryptone Soy Agar (TSA). Konsentrasi ekstrak etanol 96% daun kelor (Moringa oleifera) yang digunakan pada penelitian ini yaitu 75%, 50%, 25%, 12,5%, dan 6,25%. Data hasil penelitian dianalisis menggunakan uji one way ANOVA. Berdasarkan hasil penelitian dapat disimpulkan bahwa ekstrak etanol daun kelor (Moringa oleifera) pada konsentrasi 50% dengan waktu inkubasi 24 jam memiliki efektivitas phyto respon paling kuat terhadap virulensi Porphyromonas gingivalis ATCC 33277 secara in-vitro.Banda Ace
Awa Ndiaye Sy - One of the best experts on this subject based on the ideXlab platform.
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evaluation de l activite antioxydante des feuilles de Moringa oleifera lam Moringaceae du senegal
International Journal of Biological and Chemical Sciences, 2018Co-Authors: Awa Ndiaye Sy, A M Dieye, Alioune Diorfall, Mamadou Ndiaye, Khadim Ndiaye, Rokhaya Sylla Gueye, E Bassene, G. Y. SyAbstract:Les plantes medicinales telles que Moringa oleifera, presentent en general de nombreuses vertus therapeutiques. L’objectif de cette etude etait d’evaluer l’activite antioxydante des extraits des feuilles de Moringa oleifera par la methode de piegeage des radicaux libres 2,2-diphenyl-1- picryl-hydrazyle (DPPH•). Une extraction ethanolique a ete effectuee et l’extrait sec obtenu a ensuite ete fractionne par la methode de separation liquide-liquide avec des solvants polaires (eau distillee et acetate d’ethyle) et apolaires (dichloromethane). L’extrait ethanolique, ses fractions et l’acide L-ascorbique (antioxydant de reference), ont ete testes a differentes concentrations: 12,5; 25; 50; 100 et 200 μg/ml. La lecture de l’absorbance des produits s’est faite au bout de 30 minutes au spectrophotometre a 517 nm. Il ressort de cette etude que l’extrait ethanolique des feuilles de Moringa oleifera et ses differentes fractions possedent une activite antioxydante significative qui est dose-dependante.Cependant, l’activite la plus importante a ete observee avec l’extrait ethanolique avec une concentration inhibitrice 50 (CI50) de 87,86±1,80 μg/ml. Les resultats de cette etude suggerent que les feuilles de Moringa oleifera contiennent des substances a proprietes antioxydantes qui pourraient contribuer a la prevention de certaines maladies chroniques telles que le cancer, les maladies cardiovasculaires entre autres. © 2018 International Formulae Group. All rights reserved. Mots cles: Moringa oleifera , activite antioxydante, fractionnement, DPPH English Title: Evaluation of the antioxidant activity of leaves of Moringa oleifera Lam. (Moringaceae) of Senegal English Abstract Medicinal plants such as Moringa oleifera generally have several therapeutic virtues. This study aimed to evaluate the antioxidant activity of Moringa oleifera leaves extracts using the free radical scavenging method: 2,2-diphenyl-1-picryl-hydrazyl (DPPH•) assay. Ethanolic extraction was carried out and the dry extract obtained was then fractionated by the liquid-liquid separation method with polar solvents (distilled water and ethyl acetate) and non polar solvent (dichloromethane). The antioxidant activities of the ethanolic extract, its fractions and the Lascorbic acid (reference antioxidant) were tested at various concentrations of 12.5; 25; 50; 100 and 200 μg/ml. The absorbance of the products was read after 30 minutes at the spectrophotometer at 517 nm. It emerged from this study that the ethanolic extract of Moringa oleifera leaves and its different fractions had significant antioxidant activity that is dose-dependent. However, the ethanolic extract was more active on DPPH radical inhibition with a concentration of sample required to scavenge 50% of free radicals (CI50) of 87.86 ± 1.80 μg/ml. These results suggest that leaves of Moringa oleifera contain substances with antioxidant properties that could contribute to the prevention of chronic diseases such as cancer, cardiovascular diseases, among others. © 2018 International Formulae Group. All rights reserved. Keywords: Moringa oleifera , antioxidant activity, DPPH
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evaluation de l activite antioxydante des feuilles de Moringa oleifera lam Moringaceae du senegal
International Journal of Biological and Chemical Sciences, 2018Co-Authors: Awa Ndiaye Sy, A M Dieye, Alioune Diorfall, Mamadou Ndiaye, Khadim Ndiaye, Rokhaya Sylla Gueye, E Bassene, G. Y. SyAbstract:Les plantes medicinales telles que Moringa oleifera, presentent en general de nombreuses vertus therapeutiques. L’objectif de cette etude etait d’evaluer l’activite antioxydante des extraits des feuilles de Moringa oleifera par la methode de piegeage des radicaux libres 2,2-diphenyl-1- picryl-hydrazyle (DPPH•). Une extraction ethanolique a ete effectuee et l’extrait sec obtenu a ensuite ete fractionne par la methode de separation liquide-liquide avec des solvants polaires (eau distillee et acetate d’ethyle) et apolaires (dichloromethane). L’extrait ethanolique, ses fractions et l’acide L-ascorbique (antioxydant de reference), ont ete testes a differentes concentrations: 12,5; 25; 50; 100 et 200 μg/ml. La lecture de l’absorbance des produits s’est faite au bout de 30 minutes au spectrophotometre a 517 nm. Il ressort de cette etude que l’extrait ethanolique des feuilles de Moringa oleifera et ses differentes fractions possedent une activite antioxydante significative qui est dose-dependante.Cependant, l’activite la plus importante a ete observee avec l’extrait ethanolique avec une concentration inhibitrice 50 (CI50) de 87,86±1,80 μg/ml. Les resultats de cette etude suggerent que les feuilles de Moringa oleifera contiennent des substances a proprietes antioxydantes qui pourraient contribuer a la prevention de certaines maladies chroniques telles que le cancer, les maladies cardiovasculaires entre autres. © 2018 International Formulae Group. All rights reserved. Mots cles: Moringa oleifera , activite antioxydante, fractionnement, DPPH English Title: Evaluation of the antioxidant activity of leaves of Moringa oleifera Lam. (Moringaceae) of Senegal English Abstract Medicinal plants such as Moringa oleifera generally have several therapeutic virtues. This study aimed to evaluate the antioxidant activity of Moringa oleifera leaves extracts using the free radical scavenging method: 2,2-diphenyl-1-picryl-hydrazyl (DPPH•) assay. Ethanolic extraction was carried out and the dry extract obtained was then fractionated by the liquid-liquid separation method with polar solvents (distilled water and ethyl acetate) and non polar solvent (dichloromethane). The antioxidant activities of the ethanolic extract, its fractions and the Lascorbic acid (reference antioxidant) were tested at various concentrations of 12.5; 25; 50; 100 and 200 μg/ml. The absorbance of the products was read after 30 minutes at the spectrophotometer at 517 nm. It emerged from this study that the ethanolic extract of Moringa oleifera leaves and its different fractions had significant antioxidant activity that is dose-dependent. However, the ethanolic extract was more active on DPPH radical inhibition with a concentration of sample required to scavenge 50% of free radicals (CI50) of 87.86 ± 1.80 μg/ml. These results suggest that leaves of Moringa oleifera contain substances with antioxidant properties that could contribute to the prevention of chronic diseases such as cancer, cardiovascular diseases, among others. © 2018 International Formulae Group. All rights reserved. Keywords: Moringa oleifera , antioxidant activity, DPPH
A M Dieye - One of the best experts on this subject based on the ideXlab platform.
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evaluation de l activite antioxydante des feuilles de Moringa oleifera lam Moringaceae du senegal
International Journal of Biological and Chemical Sciences, 2018Co-Authors: Awa Ndiaye Sy, A M Dieye, Alioune Diorfall, Mamadou Ndiaye, Khadim Ndiaye, Rokhaya Sylla Gueye, E Bassene, G. Y. SyAbstract:Les plantes medicinales telles que Moringa oleifera, presentent en general de nombreuses vertus therapeutiques. L’objectif de cette etude etait d’evaluer l’activite antioxydante des extraits des feuilles de Moringa oleifera par la methode de piegeage des radicaux libres 2,2-diphenyl-1- picryl-hydrazyle (DPPH•). Une extraction ethanolique a ete effectuee et l’extrait sec obtenu a ensuite ete fractionne par la methode de separation liquide-liquide avec des solvants polaires (eau distillee et acetate d’ethyle) et apolaires (dichloromethane). L’extrait ethanolique, ses fractions et l’acide L-ascorbique (antioxydant de reference), ont ete testes a differentes concentrations: 12,5; 25; 50; 100 et 200 μg/ml. La lecture de l’absorbance des produits s’est faite au bout de 30 minutes au spectrophotometre a 517 nm. Il ressort de cette etude que l’extrait ethanolique des feuilles de Moringa oleifera et ses differentes fractions possedent une activite antioxydante significative qui est dose-dependante.Cependant, l’activite la plus importante a ete observee avec l’extrait ethanolique avec une concentration inhibitrice 50 (CI50) de 87,86±1,80 μg/ml. Les resultats de cette etude suggerent que les feuilles de Moringa oleifera contiennent des substances a proprietes antioxydantes qui pourraient contribuer a la prevention de certaines maladies chroniques telles que le cancer, les maladies cardiovasculaires entre autres. © 2018 International Formulae Group. All rights reserved. Mots cles: Moringa oleifera , activite antioxydante, fractionnement, DPPH English Title: Evaluation of the antioxidant activity of leaves of Moringa oleifera Lam. (Moringaceae) of Senegal English Abstract Medicinal plants such as Moringa oleifera generally have several therapeutic virtues. This study aimed to evaluate the antioxidant activity of Moringa oleifera leaves extracts using the free radical scavenging method: 2,2-diphenyl-1-picryl-hydrazyl (DPPH•) assay. Ethanolic extraction was carried out and the dry extract obtained was then fractionated by the liquid-liquid separation method with polar solvents (distilled water and ethyl acetate) and non polar solvent (dichloromethane). The antioxidant activities of the ethanolic extract, its fractions and the Lascorbic acid (reference antioxidant) were tested at various concentrations of 12.5; 25; 50; 100 and 200 μg/ml. The absorbance of the products was read after 30 minutes at the spectrophotometer at 517 nm. It emerged from this study that the ethanolic extract of Moringa oleifera leaves and its different fractions had significant antioxidant activity that is dose-dependent. However, the ethanolic extract was more active on DPPH radical inhibition with a concentration of sample required to scavenge 50% of free radicals (CI50) of 87.86 ± 1.80 μg/ml. These results suggest that leaves of Moringa oleifera contain substances with antioxidant properties that could contribute to the prevention of chronic diseases such as cancer, cardiovascular diseases, among others. © 2018 International Formulae Group. All rights reserved. Keywords: Moringa oleifera , antioxidant activity, DPPH
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evaluation de l activite antioxydante des feuilles de Moringa oleifera lam Moringaceae du senegal
International Journal of Biological and Chemical Sciences, 2018Co-Authors: Awa Ndiaye Sy, A M Dieye, Alioune Diorfall, Mamadou Ndiaye, Khadim Ndiaye, Rokhaya Sylla Gueye, E Bassene, G. Y. SyAbstract:Les plantes medicinales telles que Moringa oleifera, presentent en general de nombreuses vertus therapeutiques. L’objectif de cette etude etait d’evaluer l’activite antioxydante des extraits des feuilles de Moringa oleifera par la methode de piegeage des radicaux libres 2,2-diphenyl-1- picryl-hydrazyle (DPPH•). Une extraction ethanolique a ete effectuee et l’extrait sec obtenu a ensuite ete fractionne par la methode de separation liquide-liquide avec des solvants polaires (eau distillee et acetate d’ethyle) et apolaires (dichloromethane). L’extrait ethanolique, ses fractions et l’acide L-ascorbique (antioxydant de reference), ont ete testes a differentes concentrations: 12,5; 25; 50; 100 et 200 μg/ml. La lecture de l’absorbance des produits s’est faite au bout de 30 minutes au spectrophotometre a 517 nm. Il ressort de cette etude que l’extrait ethanolique des feuilles de Moringa oleifera et ses differentes fractions possedent une activite antioxydante significative qui est dose-dependante.Cependant, l’activite la plus importante a ete observee avec l’extrait ethanolique avec une concentration inhibitrice 50 (CI50) de 87,86±1,80 μg/ml. Les resultats de cette etude suggerent que les feuilles de Moringa oleifera contiennent des substances a proprietes antioxydantes qui pourraient contribuer a la prevention de certaines maladies chroniques telles que le cancer, les maladies cardiovasculaires entre autres. © 2018 International Formulae Group. All rights reserved. Mots cles: Moringa oleifera , activite antioxydante, fractionnement, DPPH English Title: Evaluation of the antioxidant activity of leaves of Moringa oleifera Lam. (Moringaceae) of Senegal English Abstract Medicinal plants such as Moringa oleifera generally have several therapeutic virtues. This study aimed to evaluate the antioxidant activity of Moringa oleifera leaves extracts using the free radical scavenging method: 2,2-diphenyl-1-picryl-hydrazyl (DPPH•) assay. Ethanolic extraction was carried out and the dry extract obtained was then fractionated by the liquid-liquid separation method with polar solvents (distilled water and ethyl acetate) and non polar solvent (dichloromethane). The antioxidant activities of the ethanolic extract, its fractions and the Lascorbic acid (reference antioxidant) were tested at various concentrations of 12.5; 25; 50; 100 and 200 μg/ml. The absorbance of the products was read after 30 minutes at the spectrophotometer at 517 nm. It emerged from this study that the ethanolic extract of Moringa oleifera leaves and its different fractions had significant antioxidant activity that is dose-dependent. However, the ethanolic extract was more active on DPPH radical inhibition with a concentration of sample required to scavenge 50% of free radicals (CI50) of 87.86 ± 1.80 μg/ml. These results suggest that leaves of Moringa oleifera contain substances with antioxidant properties that could contribute to the prevention of chronic diseases such as cancer, cardiovascular diseases, among others. © 2018 International Formulae Group. All rights reserved. Keywords: Moringa oleifera , antioxidant activity, DPPH
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contribution to the study of the anti inflammatory activity of Moringa oleifera Moringaceae
Dakar médical, 2002Co-Authors: Magatte Ndiaye, A M Dieye, Sall A Diallo, Adama Tall, Fujita Mariko, B FayeAbstract:: Moringa oleifera is a bush of African savannah, used in folk Medicine for the treatment of rheumatic and articulary pain. We have tested the anti-inflammatory action of an aqueous extract of root in rats with weight between 120 and 160 g. We administered per os either distilled water (control group), the aqueous root extract (750 mg/kg or 1000 mg/kg) or indomethacin (10 mg/kg) 30 min before an oedema was induced in the rat-paw by subcutaneous injection of carrageenin. The rat-paw volume was measured 1, 3 and 5 hours after injection of carrageenin. At a dose of 750 mg/kg the Moringa oleifera treatment significantly inhibited the development of oedema at 1, 3 and 5 hours (reduction by 53.5, 44.6 and 51.1% respectively). Increasing the dose of Moringa oleifera to 1000 mg/kg did not increase the inhibitory effect on oedema development at 1 and 3 hours, whereas this dose potentiated the oedema at 5 hours. Treatment with indomethacin significantly inhibited the development of oedema 1, 3 and 5 hours (49.1, 82.1 and 46.9% respectively). These findings indicate that an aqueous root extract of Moringa oleifera at 750 mg/kg reduces the carrageenin induced oedema to similar extent as the potent anti-inflammatory drug indomethacin. Moreover, these results provide further evidence that the roots of Moringa oleifera contain anti-inflammatory principle that may be useful in the treatment of the acute inflammatory conditions.