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Craig V. Sullivan - One of the best experts on this subject based on the ideXlab platform.
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a microsatellite linkage map of striped bass Morone saxatilis reveals conserved synteny with the three spined stickleback gasterosteus aculeatus
Marine Biotechnology, 2012Co-Authors: Sixin Liu, Charlene R. Couch, Caird E. Rexroad, Jan F Cordes, Kimberly S Reece, Craig V. SullivanAbstract:The striped bass (Morone saxatilis) and its relatives (genus Morone) are of great importance to fisheries and aquaculture in North America. As part of a collaborative effort to employ molecular genetics technologies in striped bass breeding programs, we previously developed nearly 500 microsatellite markers. The objectives of this study were to construct a microsatellite linkage map of striped bass and to examine conserved synteny between striped bass and three-spined stickleback (Gasterosteus aculeatus). Of 480 microsatellite markers screened for polymorphism, 289 informative markers were identified and used to genotype two half-sib mapping families. Twenty-six linkage groups were assembled, and only two markers remain unlinked. The sex-averaged map spans 1,623.8 cM with an average marker density of 5.78 cM per marker. Among 287 striped bass microsatellite markers assigned to linkage groups, 169 (58.9%) showed homology to sequences on stickleback chromosomes or scaffolds. Comparison between the stickleback genome and the striped bass linkage map revealed conserved synteny between these two species. This is the first linkage map for any of the Morone species. This map will be useful for molecular mapping and marker-assisted selection of genes of interest in striped bass breeding programs. The conserved synteny between striped bass and stickleback will facilitate fine mapping of genome regions of interest and will serve as a new resource for comparative mapping with other Perciform fishes such as European sea bass (Dicentrarchus labrax), gilthead sea bream (Sparus aurata), and tilapia (Oreochromis ssp.).
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Conserved and Variant Molecular and Functional Features of Multiple Egg Yolk Precursor Proteins (Vitellogenins) in White Perch (Morone americana) and other Teleosts
Marine Biotechnology, 2008Co-Authors: Benjamin J. Reading, Naoshi Hiramatsu, Sayumi Sawaguchi, Takahiro Matsubara, Akihiko Hara, Mark O. Lively, Craig V. SullivanAbstract:Three complete cDNAs encoding different forms of vitellogenin (Vtg) were isolated from a white perch ( Morone americana ) liver cDNA library and characterized with respect to immunobiochemical and functional features of the three Vtgs and their product yolk proteins (YPs) in this species and in the congeneric striped bass ( Morone saxatilis ). The two longest cDNAs encoded Vtgs with a complete suite of yolk protein domains that, based on comparisons with vtg sequences from other species, were categorized as VtgAa and VtgAb using the current nomenclature for multiple teleost Vtgs. The shorter cDNA encoded a Vtg that lacked a phosvitin domain, had a shortened C-terminus, and was categorized as VtgC. Mapping of peptide sequences from the purified Vtgs and their derived YPs to Vtg sequences deduced from the cDNAs definitively identified the white perch VtgAa, VtgAb, and VtgC proteins. Detailed comparisons of the primary structures of each Vtg with partial or complete sequences of Morone yolk proteins or of Vtgs from other fishes revealed conserved and variant structural elements of teleost Vtgs with functional significance, including, as examples, signal peptide cleavage sites, dimerization sites, cathepsin D protease recognition sites, and receptor-binding domains. These comparisons also yielded an interim revision of the classification scheme for multiple teleost Vtgs.
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Isolation and characterization of 149 novel microsatellite DNA markers for striped bass, Morone saxatilis, and cross-species amplification in white bass, Morone chrysops, and their hybrid
Molecular Ecology Notes, 2006Co-Authors: Charlene R. Couch, Amber F. Garber, Caird E. Rexroad, J. M. Abrams, J. A. Stannard, M. E. Westerman, Craig V. SullivanAbstract:To support detailed genetic analysis of striped bass ( Morone saxatilis ) and white bass ( Morone chrysops ), we isolated 153 microsatellite loci from repeat-enriched striped bass DNA libraries. Of these, 147 markers amplified in striped bass (average 4.7 alleles per locus) and 133 in white bass (average 2.2 alleles per locus). One hundred twenty-two markers amplified in their hybrid. Development of new microsatellite markers will facilitate evaluations of genetic structure in wild populations and will support pedigree analysis and linkage mapping for selective breeding.
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Courtship and Tank Spawning Behavior of Temperate Basses (Genus Morone)
Transactions of the American Fisheries Society, 2001Co-Authors: Stephen J Salek, Craig V. Sullivan, John Godwin, N. E. StaceyAbstract:Abstract Special arenas were used to observe and describe courtship and spawning behavior of captive striped bass Morone saxatilis, white bass Morone chrysops, and white perch Morone americana. To induce final gonadal maturation and spawning, fish were either implanted with gonadotropin-releasing hormone analog, injected with human chorionic gonadotropin, or both. Behaviors were videotaped and systematically quantified. Broodfish displayed courtship behavior for at least 5 h before spawning, characterized by one female and from one to five males releasing gametes at the water surface. Spawning lasted about 10 s for striped bass, 5 s for white bass, and less than 1 s for white perch. The best predictor of imminent spawning was a significant increase in male attending behavior, defined as extremely close and continuous following of the female, sometimes contacting her abdominal or vent area with the snout. Around the time of spawning, male striped bass attended females less intensely than did white bass or ...
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Morpho-physiological predictors of ovulatory success in captive striped bass (Morone saxatilis)
Aquaculture, 2000Co-Authors: Gregory M. Weber, William King, Robert W. Clark, Ronald G. Hodson, Craig V. SullivanAbstract:This study evaluates morpho-physiological characters as predictors of ovulatory success in cultured striped bass, Morone saxatilis, that could be used by farmers to select females for induced spawning. Diameter, size homogeneity and growth of ovarian follicles; blood plasma . . . levels of testosterone T , oestradiol-17b E and vitellogenin VTG ; and in vitro maturation of 2 .
Eric Peatman - One of the best experts on this subject based on the ideXlab platform.
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Hepatic transcriptomic and metabolic responses of hybrid striped bass (Morone saxatilis×Morone chrysops) to acute and chronic hypoxic insult.
Comparative biochemistry and physiology. Part D Genomics & proteomics, 2016Co-Authors: Benjamin H. Beck, Carl D. Webster, S. Adam Fuller, Bartholomew W. Green, Honggang Zhao, Steven D. Rawles, Eric PeatmanAbstract:Striped bass (Morone saxatilis), white bass (Morone chrysops), and their hybrid are an important group of fish prized for recreational angling in the United States, and there and abroad as a high-value farmed fish. Regardless of habitat, it is not uncommon for fish of the genus Morone to encounter and cope with conditions of scarce oxygen availability. Previously, we determined that hybrid striped bass reared under conditions of chronic hypoxia exhibited reduced feed intake, lower lipid and nutrient retention, and poor growth. To better understand the molecular mechanisms governing these phenotypes, in the present study, we examined the transcriptomic profiles of hepatic tissue in hybrid striped bass exposed to chronic hypoxia (90days at 25% oxygen saturation) and acute hypoxia (6h at 25% oxygen saturation). Using high-throughput RNA-seq, we found that over 1400 genes were differentially expressed under disparate oxygen conditions, with the vast majority of transcriptional changes occurring in the acute hypoxia treatment. Gene pathway and bioenergetics analyses revealed hypoxia-mediated perturbation of genes and gene networks related to lipid metabolism, cell death, and changes in hepatic mitochondrial content and cellular respiration. This study offers a more comprehensive view of the temporal and tissue-specific transcriptional changes that occur during hypoxia, and reveals new and shared mechanisms of hypoxia tolerance in teleosts.
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Transcriptome annotation and marker discovery in white bass (Morone chrysops) and striped bass (Morone saxatilis)
Animal genetics, 2014Co-Authors: Benjamin H. Beck, S. Adam Fuller, Eric PeatmanAbstract:Summary Striped bass (Morone saxatilis) and white bass (Morone chrysops) are the parental species of the hybrid striped bass, a major U.S. aquaculture species. Currently, genomic resources for striped bass, white bass, and their hybrid lag behind those of other aquaculture species. Current resources consist of a medium-density genetic linkage map and a well-annotated ovarian transcriptome. A well-annotated transcriptome from across striped bass and white bass tissues is needed to advance both broad-based RNA-seq studies of gene expression as well as aid in more targeted studies of important genes and pathways critical for reproductive physiology and immunity. Here, we carried out Illumina-based transcriptome sequencing and annotation in both species utilizing the trinity and trinotate packages. The assembled Moronid reference transcriptomes and identified SSRs and SNPs should advance ongoing studies of reproduction, physiology, and immunology in these species and provide markers for broodstock management and selection.
Isaac Wirgin - One of the best experts on this subject based on the ideXlab platform.
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Mixed-stock analysis of Atlantic coast striped bass (Morone saxatilis) using nuclear DNA and mitochondrial DNA markers
Canadian Journal of Fisheries and Aquatic Sciences, 1997Co-Authors: Isaac Wirgin, Lorraine Maceda, John R. Waldman, Joseph Stabile, Victor J. VecchioAbstract:Striped bass (Morone saxatilis) stocks comingle along the northeastern United States and Canadian coasts and support mixed-stock fisheries in which stock compositions fluctuate widely. Many approac...
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Mitochondrial DNA Variation in Striped Bass (Morone saxatilis) from Canadian Rivers
Canadian Journal of Fisheries and Aquatic Sciences, 1993Co-Authors: Isaac Wirgin, Tun-liang Ong, Lorraine Maceda, John R. Waldman, David Moored. Moore, Simon C. CourtenayAbstract:Mitochondrial DNA(mtDNA)was analysed to determine the genetic relatedness of striped bass (Morone saxatilis) populations in tributaries to the Gulf of St. Lawrence and the Bay of Funday. Mitochondr...
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Use of Cellular Oncogene Probes to Identify Morone Hybrids
The Journal of heredity, 1992Co-Authors: Isaac Wirgin, Lorraine Maceda, C. MesingAbstract:We tested the ability of cellular oncogene (c-onc) probes to identify F1 hybrids and the lineage of known backcrosses within the fish genus Morone. Total DNA was isolated from five to 14 individuals per North American Morone species (striped bass, white bass, white perch, and yellow bass). The DNA was digested with two restriction enzymes, Eco RI and Hin dIII, Southern blotted, and hybridized to six different c-onc probes including v-abl, v-erb B, c-myc, c-H-ras, c-K-ras, and v-src. We found fixed genotypic differences among the four species for all six probes in single restriction enzyme digests. The heritability of these nuclear DNA genotypes was evaluated in hatchery-produced F1 Morone hybrids (striped bass x white bass and striped bass x white perch) tested with the six informative single probe/restriction enzyme combinations. All F1 individuals exhibited heterozygosity in all diagnostic nuclear DNA fragments, confirming the Mendelian inheritance of these genotypes in these fish. Furthermore, analysis of these nuclear DNA genotypes in hatchery-produced backcrosses of F1 hybrids striped bass x (white bass x striped bass) detected both recombinant and parental genotypes at all six polymorphic c-onc sequences. The lineage of suspected Morone hybrids of unknown descent collected from Lewis Smith Lake, Alabama, and from the Occoquan River, Virginia, was determined using the c-onc probes. Our results suggest that c-onc probes are suitable markers to unequivocally identify F1 hybrids and backcrosses and to quantify introgression in natural populations of fishes. The addition of RFLP analysis of mtDNA provided a complete ancestral history of individual fish.
Benjamin H. Beck - One of the best experts on this subject based on the ideXlab platform.
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Hepatic transcriptomic and metabolic responses of hybrid striped bass (Morone saxatilis×Morone chrysops) to acute and chronic hypoxic insult.
Comparative biochemistry and physiology. Part D Genomics & proteomics, 2016Co-Authors: Benjamin H. Beck, Carl D. Webster, S. Adam Fuller, Bartholomew W. Green, Honggang Zhao, Steven D. Rawles, Eric PeatmanAbstract:Striped bass (Morone saxatilis), white bass (Morone chrysops), and their hybrid are an important group of fish prized for recreational angling in the United States, and there and abroad as a high-value farmed fish. Regardless of habitat, it is not uncommon for fish of the genus Morone to encounter and cope with conditions of scarce oxygen availability. Previously, we determined that hybrid striped bass reared under conditions of chronic hypoxia exhibited reduced feed intake, lower lipid and nutrient retention, and poor growth. To better understand the molecular mechanisms governing these phenotypes, in the present study, we examined the transcriptomic profiles of hepatic tissue in hybrid striped bass exposed to chronic hypoxia (90days at 25% oxygen saturation) and acute hypoxia (6h at 25% oxygen saturation). Using high-throughput RNA-seq, we found that over 1400 genes were differentially expressed under disparate oxygen conditions, with the vast majority of transcriptional changes occurring in the acute hypoxia treatment. Gene pathway and bioenergetics analyses revealed hypoxia-mediated perturbation of genes and gene networks related to lipid metabolism, cell death, and changes in hepatic mitochondrial content and cellular respiration. This study offers a more comprehensive view of the temporal and tissue-specific transcriptional changes that occur during hypoxia, and reveals new and shared mechanisms of hypoxia tolerance in teleosts.
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Transcriptome annotation and marker discovery in white bass (Morone chrysops) and striped bass (Morone saxatilis)
Animal genetics, 2014Co-Authors: Benjamin H. Beck, S. Adam Fuller, Eric PeatmanAbstract:Summary Striped bass (Morone saxatilis) and white bass (Morone chrysops) are the parental species of the hybrid striped bass, a major U.S. aquaculture species. Currently, genomic resources for striped bass, white bass, and their hybrid lag behind those of other aquaculture species. Current resources consist of a medium-density genetic linkage map and a well-annotated ovarian transcriptome. A well-annotated transcriptome from across striped bass and white bass tissues is needed to advance both broad-based RNA-seq studies of gene expression as well as aid in more targeted studies of important genes and pathways critical for reproductive physiology and immunity. Here, we carried out Illumina-based transcriptome sequencing and annotation in both species utilizing the trinity and trinotate packages. The assembled Moronid reference transcriptomes and identified SSRs and SNPs should advance ongoing studies of reproduction, physiology, and immunology in these species and provide markers for broodstock management and selection.
Lorraine Maceda - One of the best experts on this subject based on the ideXlab platform.
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Mixed-stock analysis of Atlantic coast striped bass (Morone saxatilis) using nuclear DNA and mitochondrial DNA markers
Canadian Journal of Fisheries and Aquatic Sciences, 1997Co-Authors: Isaac Wirgin, Lorraine Maceda, John R. Waldman, Joseph Stabile, Victor J. VecchioAbstract:Striped bass (Morone saxatilis) stocks comingle along the northeastern United States and Canadian coasts and support mixed-stock fisheries in which stock compositions fluctuate widely. Many approac...
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Mitochondrial DNA Variation in Striped Bass (Morone saxatilis) from Canadian Rivers
Canadian Journal of Fisheries and Aquatic Sciences, 1993Co-Authors: Isaac Wirgin, Tun-liang Ong, Lorraine Maceda, John R. Waldman, David Moored. Moore, Simon C. CourtenayAbstract:Mitochondrial DNA(mtDNA)was analysed to determine the genetic relatedness of striped bass (Morone saxatilis) populations in tributaries to the Gulf of St. Lawrence and the Bay of Funday. Mitochondr...
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Use of Cellular Oncogene Probes to Identify Morone Hybrids
The Journal of heredity, 1992Co-Authors: Isaac Wirgin, Lorraine Maceda, C. MesingAbstract:We tested the ability of cellular oncogene (c-onc) probes to identify F1 hybrids and the lineage of known backcrosses within the fish genus Morone. Total DNA was isolated from five to 14 individuals per North American Morone species (striped bass, white bass, white perch, and yellow bass). The DNA was digested with two restriction enzymes, Eco RI and Hin dIII, Southern blotted, and hybridized to six different c-onc probes including v-abl, v-erb B, c-myc, c-H-ras, c-K-ras, and v-src. We found fixed genotypic differences among the four species for all six probes in single restriction enzyme digests. The heritability of these nuclear DNA genotypes was evaluated in hatchery-produced F1 Morone hybrids (striped bass x white bass and striped bass x white perch) tested with the six informative single probe/restriction enzyme combinations. All F1 individuals exhibited heterozygosity in all diagnostic nuclear DNA fragments, confirming the Mendelian inheritance of these genotypes in these fish. Furthermore, analysis of these nuclear DNA genotypes in hatchery-produced backcrosses of F1 hybrids striped bass x (white bass x striped bass) detected both recombinant and parental genotypes at all six polymorphic c-onc sequences. The lineage of suspected Morone hybrids of unknown descent collected from Lewis Smith Lake, Alabama, and from the Occoquan River, Virginia, was determined using the c-onc probes. Our results suggest that c-onc probes are suitable markers to unequivocally identify F1 hybrids and backcrosses and to quantify introgression in natural populations of fishes. The addition of RFLP analysis of mtDNA provided a complete ancestral history of individual fish.