The Experts below are selected from a list of 27192 Experts worldwide ranked by ideXlab platform
Koki Horikoshi - One of the best experts on this subject based on the ideXlab platform.
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preparation and characterisation of nanofibrous cellulose plate as a new solid support for microbial culture
Soft Matter, 2007Co-Authors: Shigeru Deguchi, Mikiko Tsudome, Yihong Shen, Kaoru Tsujii, Koki HorikoshiAbstract:Porous plates made of nanofibrous crystalline cellulose were prepared, and used as a solid support for microbial cultures. Representative mesophilic microorganisms (Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae) grew on the cellulose plate, just as well as they did on the conventional agar plate. In situ optical microscopic examination revealed that the cellulose plate remained unchanged up to 280 °C at a constant pressure of 25 MPa. Due to the structural stability at high temperatures, a representative thermophile, Thermus thermophilus, was cultured successfully on the cellulose plate at 80 °C. Mouse Fibroblast cells did not show significant adhesion or extension on the cellulose plate.
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preparation and characterisation of nanofibrous cellulose plate as a new solid support for microbial culture
Soft Matter, 2007Co-Authors: Shigeru Deguchi, Mikiko Tsudome, Yihong Shen, Kaoru Tsujii, Satoshi Konishi, Susumu Ito, Koki HorikoshiAbstract:Porous plates made of nanofibrous crystalline cellulose were prepared, and used as a solid support for microbial cultures. Representative mesophilic microorganisms (, , and ) grew on the cellulose plate, just as well as they did on the conventional agar plate. optical microscopic examination revealed that the cellulose plate remained unchanged up to 280 °C at a constant pressure of 25 MPa. Due to the structural stability at high temperatures, a representative thermophile, , was cultured successfully on the cellulose plate at 80 °C. Mouse Fibroblast cells did not show significant adhesion or extension on the cellulose plate.
Shigeru Deguchi - One of the best experts on this subject based on the ideXlab platform.
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preparation and characterisation of nanofibrous cellulose plate as a new solid support for microbial culture
Soft Matter, 2007Co-Authors: Shigeru Deguchi, Mikiko Tsudome, Yihong Shen, Kaoru Tsujii, Koki HorikoshiAbstract:Porous plates made of nanofibrous crystalline cellulose were prepared, and used as a solid support for microbial cultures. Representative mesophilic microorganisms (Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae) grew on the cellulose plate, just as well as they did on the conventional agar plate. In situ optical microscopic examination revealed that the cellulose plate remained unchanged up to 280 °C at a constant pressure of 25 MPa. Due to the structural stability at high temperatures, a representative thermophile, Thermus thermophilus, was cultured successfully on the cellulose plate at 80 °C. Mouse Fibroblast cells did not show significant adhesion or extension on the cellulose plate.
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preparation and characterisation of nanofibrous cellulose plate as a new solid support for microbial culture
Soft Matter, 2007Co-Authors: Shigeru Deguchi, Mikiko Tsudome, Yihong Shen, Kaoru Tsujii, Satoshi Konishi, Susumu Ito, Koki HorikoshiAbstract:Porous plates made of nanofibrous crystalline cellulose were prepared, and used as a solid support for microbial cultures. Representative mesophilic microorganisms (, , and ) grew on the cellulose plate, just as well as they did on the conventional agar plate. optical microscopic examination revealed that the cellulose plate remained unchanged up to 280 °C at a constant pressure of 25 MPa. Due to the structural stability at high temperatures, a representative thermophile, , was cultured successfully on the cellulose plate at 80 °C. Mouse Fibroblast cells did not show significant adhesion or extension on the cellulose plate.
Yukio Imanishi - One of the best experts on this subject based on the ideXlab platform.
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serum free cell culture on insulin immobilized porous collagen beads
Biotechnology and Bioengineering, 1995Co-Authors: Yoshihiro Ito, Ji Zheng, Yukio ImanishiAbstract:Insulin or albumin was immobilized on collagen beads using water-soluble carbodiimide. Adhesion of STO Mouse Fibroblast cells onto the beads decreased with increasing the amount of immobilized proteins. Growth of the cells was remarkably accelerated on the insulinimmobilized collagen beads, which can be used for serum-free cell culture. The growth acceleration became larger with increasing the amount of immobilized insulin, while it became smaller with increasing the amount of immobilized albumin. In addition, the immobilized insulin more strongly accelerated the cell growth than free insulin plus collagen beads. (c) 1995 John Wiley & Sons, Inc.
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cell growth on immobilized cell growth factor 7 protein free cell culture by using growth factor immobilized polymer membrane
Enzyme and Microbial Technology, 1993Co-Authors: Shu Qin Liu, Yoshihiro Ito, Yukio ImanishiAbstract:Abstract A protein-free culture of anchorage-dependent cells, Mouse Fibroblast cells, STO and 3T3-LI and fibroic sarcoma cells, Swiss albino HSDM 1 C 1 , grown on a cell-growth protein, insulin, and/or a cell-adhesion protein, collagen, which are immobilized or coimmobilized on surface-hydrolyzed poly(methyl methacrylate) membrane, was investigated. By adding metal ions and lipids to the culture medium, a proteinfree culture medium was composed, which was potent in promoting cell proliferation similarly to serum-containing culture medium. In particular, with insulin/collagen-coimmobilized membrane, a protein-free culture was established without detachment of growing cells over a long period. These protein-immobilized membranes could be used repeatedly.
E R Nestmann - One of the best experts on this subject based on the ideXlab platform.
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recommended protocols based on a survey of current practice in genotoxicity testing laboratories iii cell transformation in c3h 10t1 2 Mouse embryo cell balb c 3t3 Mouse Fibroblast and syrian hamster embryo cell cultures
Mutation Research, 1991Co-Authors: V C Dunkel, C G Rogers, Sabine H H Swierenga, R L Brillinger, J P W Gilman, E R NestmannAbstract:A standardized protocol and guidelines for the performance of cell transformation testing in Mouse embryo (C3H/10T1/2), Mouse Fibroblast (BALB/c 3T3) and Syrian hamster embryo (SHE) cells have been developed. The protocol is based primarily on current laboratory practices as determined by responses to a detailed questionnaire completed by North American and European governmental, university and contract laboratories involved with cell transformation experimentation. This report identifies those modifications to previously described methodologies which are being used on a regular basis and also serves to clarify confusing or inconsistent practices.
Guoqiang Chen - One of the best experts on this subject based on the ideXlab platform.
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in vitro effect of oligo hydroxyalkanoates on the growth of Mouse Fibroblast cell line l929
Biomaterials, 2007Co-Authors: Jie Sun, Guoqiang Chen, Zhongwei Dai, Yan ZhaoAbstract:The cellular responses to polyhydroxyalkanoates (PHA) degradation products oligo-hydroxyalkanoates (OHAs) are very important factors that control the biocompatibility of these polymers when they are used in tissue-engineering applications. In this study, oligo(3-hydroxybutyrate) (OHB, Mn 2000), oligo(3-hydroxybutyrate-co-4-hydroxybutyrate) (O3HB4HB, Mn 2100, 6 mol% 4HB), oligo(3-hydroxybutyrate-co-3-hydroxyhexanoate) (OHBHHx, Mn 2800, 12 mol% 3HHx) and medium-chain-length oligo(3-hydroxyalkanoates) (OmclHAs, Mn 2300, 2 mol% 3-hydroxyhexanoate (3HHx), 25 mol% 3-hydroxyoctanoate, 71 mol% 3-hydroxydecanoate and 3 mol% 3-hydroxydodecanoate) were prepared as insoluble particles in aqueous solution. Liposomes were employed to encapsulate OHAs and facilitate their transfer into the cytosol. The methylthiazol tetrazolium (MTT) assay, a cell apoptosis study and the flow cytometry were used to evaluate cell viability of Mouse Fibroblast L929. OHAs in concentration lower than 20mg/l did not significantly affect cell viability, while OHAs over 40 mg/l reduced cell viability with more cell apoptosis, more cell death, delayed cell cycle and reduced cell proliferation. The cytotoxicity of OHAs decreased with increasing OHAs side chain length. It is proposed that medium-chain-length OHAs containing PHA, such as poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) and medium-chain-length PHA Poly(3-hydroxyalkanoates) (mcl PHA), are preferred for development and application as PHA-based tissue-engineering biomaterials.
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reduced Mouse Fibroblast cell growth by increased hydrophilicity of microbial polyhydroxyalkanoates via hyaluronan coating
Biomaterials, 2003Co-Authors: Yawu Wang, Guoqiang ChenAbstract:The Mouse Fibroblast cell line L929 was inoculated on 3D scaffolds of microbial polyesters, namely polyhydroxybutyrate (PHB) and poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) to evaluate their in vitro biocompatibility. It was found that both polyhydroxyalkanoates (PHA) subjected to lipase treatment and hyaluronan (HA) coating decreased the contact angle of water to the material surface approximately 30%, meaning an increased hydrophilicity on the PHA surface. At the same time, both the lipase treatment and the HA coating smoothened the PHA surface. After the lipase treatment or HA coating, the ratio of PHA hydrophilic groups including hydroxyl and carboxyl to carbonyl of PHA was approximately 1:1 or 2:1. Cells grown on scaffolds treated with lipase were approximately 4 x 10(5)/ml, twice in number of the control. However, PHA scaffolds coated with HA were observed with a 40% decrease in cell growth compared with that of the control. HA coating reduced the cell attachment and proliferation on PHA although the materials had increased hydrophilicity. In comparison, lipase treatment promoted the cell growth on PHA although the treatment did not lead to better hydrophilicity compared with HA coating. It appeared that an appropriate combination of hydrophilicity and hydrophobicity was important for the biocompatibility of PHBHHx, especially for the growth of L929 cells on the surface of this material. This may have instructive significance for biomaterial selection and design.