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Adel K Elnaggar - One of the best experts on this subject based on the ideXlab platform.
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primary intraosseous Mucoepidermoid Carcinoma of the jaw reappraisal of the md anderson cancer center experience
Head and Neck-journal for The Sciences and Specialties of The Head and Neck, 2016Co-Authors: Diana Bell, Carol M Lewis, Adel K Elnaggar, Randal S WeberAbstract:Background Mucoepidermoid Carcinoma arises from major or minor salivary glands, making up 10% of salivary gland tumors. Intraosseous Mucoepidermoid Carcinomas are rare, and make up only 2% to 3% of all Mucoepidermoid Carcinomas. The t(11;19) and its CRTC1-MAML2 fusion gene transcript have been identified in Mucoepidermoid Carcinoma and are associated with a subset of Mucoepidermoid Carcinomas. The extent to which the transcript influences disease features and patient survival is unclear. Methods We conducted a retrospective analysis of records for clinical features, surgical interventions, and prognoses. Reverse transcriptase-polymerase chain reaction (RT-PCR) and fluorescence in situ hybridization (FISH) used to assess the t(11;19) fusion gene in intraosseous Mucoepidermoid Carcinoma. Results Twenty-five patients with intraosseous Mucoepidermoid Carcinoma treated between 1998 and 2013 were identified. The t(11;19) fusion gene transcript CRTC1-MAML2 manifested in 9 intraosseous Mucoepidermoid Carcinomas, whereas is was not detected in another 9 intraosseous Carcinomas. Although the incidence of this fusion in Mucoepidermoid Carcinoma varies, it is generally accepted that more than 50% of this entity manifest the CRTC1-MAML2. Conclusion Intraosseous Mucoepidermoid Carcinoma diagnosis should be based on clinical and pathologic manifestations and complete resection is the first choice for patient treatment. The need for neck dissection and adjuvant treatment are debatable. Radiotherapy may improve prognosis and may be recommended in the postoperative period. Primary intraosseous Mucoepidermoid Carcinoma can manifest the fusion transcript in a subset of tumors. © 2015 Wiley Periodicals, Inc. Head Neck, 2015
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primacy of surgery in the management of Mucoepidermoid Carcinoma in children
Head and Neck-journal for The Sciences and Specialties of The Head and Neck, 2011Co-Authors: Jesse T Ryan, Adel K Elnaggar, Randal S Weber, Winston W Huh, Ehab Y Hanna, Michael E KupfermanAbstract:Background. Epithelial salivary gland neoplasms are rare in children. Malignant tumors account for 30% to 50% of cases in the pediatric age group, with Mucoepidermoid Carcinoma as the most common histology. Methods. A retrospective medical record review was con- ducted from 1953 to 2007 to identify patients with mucoepider- moid Carcinoma at the age of 18 years or younger at the time of diagnosis. Forty-nine patients were identified. Their medical records were examined for presentation, treatment, pathologic features, and outcomes. Results. Forty-nine pediatric patients with Mucoepidermoid Carcinoma were identified. The parotid gland (49%) and oral cavity (35%) were the most common subsites. Nodal meta- stasis was seen in 24% of patients. All patients underwent surgery, and 11 patients (22%) were treated with radiation therapy. The 5-year overall survival was 98%, the 10-year over- all survival was 94%, and 10% of patients developed recurrence. Conclusion. Mucoepidermoid Carcinoma in children carries a favorable prognosis and can be successfully treated with surgery alone in most cases. V C 2011 Wiley Periodicals, Inc.* Head Neck 33: 1769-1773, 2011
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crtc1 maml2 fusion transcript in warthin s tumor and Mucoepidermoid Carcinoma evidence for a common genetic association
Genes Chromosomes and Cancer, 2008Co-Authors: Diana Bell, Randal S Weber, Mario A Luna, Frederic J Kaye, Adel K ElnaggarAbstract:Translocations and gene fusions have an important early role in tumorigenesis. The t(11;19) translocation and its CRTC1/MAML2 fusion transcript have been identified in several examples of both Warthin's tumor and Mucoepidermoid Carcinoma and are believed to be associated with the development of a subset of these tumors. To determine whether Warthin's tumor and Mucoepidermoid Carcinoma are genetically related, we used reverse transcriptase-polymerase chain reaction and DNA sequencing to analyze microdissected components of three tumors consisting of Warthin's tumor and Mucoepidermoid Carcinoma. We also investigated a metastatic melanoma to Warthin's tumor and a Warthin's Carcinoma of the parotid gland for comparison. The fusion transcript was identified in both Warthin's tumor and matching Mucoepidermoid Carcinoma components of all three tumors, in the Warthin's Carcinoma, and in the Warthin's tumor component but not in the metastatic melanoma. The results provide evidence for a link between the t(11;19) fusion gene and the development of a subset of Warthin's tumors with concurrent Mucoepidermoid Carcinoma and possible malignant transformation to Warthin's Carcinoma. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat.
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crtc1 maml2 fusion transcript in warthin s tumor and Mucoepidermoid Carcinoma evidence for a common genetic association
Genes Chromosomes and Cancer, 2008Co-Authors: Diana Bell, Randal S Weber, Mario A Luna, Frederic J Kaye, Adel K ElnaggarAbstract:Translocations and gene fusions have an important early role in tumorigenesis. The t(11;19) translocation and its CRTC1/MAML2 fusion transcript have been identified in several examples of both Warthin's tumor and Mucoepidermoid Carcinoma and are believed to be associated with the development of a subset of these tumors. To determine whether Warthin's tumor and Mucoepidermoid Carcinoma are genetically related, we used reverse transcriptase-polymerase chain reaction and DNA sequencing to analyze microdissected components of three tumors consisting of Warthin's tumor and Mucoepidermoid Carcinoma. We also investigated a metastatic melanoma to Warthin's tumor and a Warthin's Carcinoma of the parotid gland for comparison. The fusion transcript was identified in both Warthin's tumor and matching Mucoepidermoid Carcinoma components of all three tumors, in the Warthin's Carcinoma, and in the Warthin's tumor component but not in the metastatic melanoma. The results provide evidence for a link between the t(11;19) fusion gene and the development of a subset of Warthin's tumors with concurrent Mucoepidermoid Carcinoma and possible malignant transformation to Warthin's Carcinoma. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat. © 2008 Wiley-Liss, Inc.
Randal S Weber - One of the best experts on this subject based on the ideXlab platform.
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primary intraosseous Mucoepidermoid Carcinoma of the jaw reappraisal of the md anderson cancer center experience
Head and Neck-journal for The Sciences and Specialties of The Head and Neck, 2016Co-Authors: Diana Bell, Carol M Lewis, Adel K Elnaggar, Randal S WeberAbstract:Background Mucoepidermoid Carcinoma arises from major or minor salivary glands, making up 10% of salivary gland tumors. Intraosseous Mucoepidermoid Carcinomas are rare, and make up only 2% to 3% of all Mucoepidermoid Carcinomas. The t(11;19) and its CRTC1-MAML2 fusion gene transcript have been identified in Mucoepidermoid Carcinoma and are associated with a subset of Mucoepidermoid Carcinomas. The extent to which the transcript influences disease features and patient survival is unclear. Methods We conducted a retrospective analysis of records for clinical features, surgical interventions, and prognoses. Reverse transcriptase-polymerase chain reaction (RT-PCR) and fluorescence in situ hybridization (FISH) used to assess the t(11;19) fusion gene in intraosseous Mucoepidermoid Carcinoma. Results Twenty-five patients with intraosseous Mucoepidermoid Carcinoma treated between 1998 and 2013 were identified. The t(11;19) fusion gene transcript CRTC1-MAML2 manifested in 9 intraosseous Mucoepidermoid Carcinomas, whereas is was not detected in another 9 intraosseous Carcinomas. Although the incidence of this fusion in Mucoepidermoid Carcinoma varies, it is generally accepted that more than 50% of this entity manifest the CRTC1-MAML2. Conclusion Intraosseous Mucoepidermoid Carcinoma diagnosis should be based on clinical and pathologic manifestations and complete resection is the first choice for patient treatment. The need for neck dissection and adjuvant treatment are debatable. Radiotherapy may improve prognosis and may be recommended in the postoperative period. Primary intraosseous Mucoepidermoid Carcinoma can manifest the fusion transcript in a subset of tumors. © 2015 Wiley Periodicals, Inc. Head Neck, 2015
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primacy of surgery in the management of Mucoepidermoid Carcinoma in children
Head and Neck-journal for The Sciences and Specialties of The Head and Neck, 2011Co-Authors: Jesse T Ryan, Adel K Elnaggar, Randal S Weber, Winston W Huh, Ehab Y Hanna, Michael E KupfermanAbstract:Background. Epithelial salivary gland neoplasms are rare in children. Malignant tumors account for 30% to 50% of cases in the pediatric age group, with Mucoepidermoid Carcinoma as the most common histology. Methods. A retrospective medical record review was con- ducted from 1953 to 2007 to identify patients with mucoepider- moid Carcinoma at the age of 18 years or younger at the time of diagnosis. Forty-nine patients were identified. Their medical records were examined for presentation, treatment, pathologic features, and outcomes. Results. Forty-nine pediatric patients with Mucoepidermoid Carcinoma were identified. The parotid gland (49%) and oral cavity (35%) were the most common subsites. Nodal meta- stasis was seen in 24% of patients. All patients underwent surgery, and 11 patients (22%) were treated with radiation therapy. The 5-year overall survival was 98%, the 10-year over- all survival was 94%, and 10% of patients developed recurrence. Conclusion. Mucoepidermoid Carcinoma in children carries a favorable prognosis and can be successfully treated with surgery alone in most cases. V C 2011 Wiley Periodicals, Inc.* Head Neck 33: 1769-1773, 2011
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crtc1 maml2 fusion transcript in warthin s tumor and Mucoepidermoid Carcinoma evidence for a common genetic association
Genes Chromosomes and Cancer, 2008Co-Authors: Diana Bell, Randal S Weber, Mario A Luna, Frederic J Kaye, Adel K ElnaggarAbstract:Translocations and gene fusions have an important early role in tumorigenesis. The t(11;19) translocation and its CRTC1/MAML2 fusion transcript have been identified in several examples of both Warthin's tumor and Mucoepidermoid Carcinoma and are believed to be associated with the development of a subset of these tumors. To determine whether Warthin's tumor and Mucoepidermoid Carcinoma are genetically related, we used reverse transcriptase-polymerase chain reaction and DNA sequencing to analyze microdissected components of three tumors consisting of Warthin's tumor and Mucoepidermoid Carcinoma. We also investigated a metastatic melanoma to Warthin's tumor and a Warthin's Carcinoma of the parotid gland for comparison. The fusion transcript was identified in both Warthin's tumor and matching Mucoepidermoid Carcinoma components of all three tumors, in the Warthin's Carcinoma, and in the Warthin's tumor component but not in the metastatic melanoma. The results provide evidence for a link between the t(11;19) fusion gene and the development of a subset of Warthin's tumors with concurrent Mucoepidermoid Carcinoma and possible malignant transformation to Warthin's Carcinoma. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat.
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crtc1 maml2 fusion transcript in warthin s tumor and Mucoepidermoid Carcinoma evidence for a common genetic association
Genes Chromosomes and Cancer, 2008Co-Authors: Diana Bell, Randal S Weber, Mario A Luna, Frederic J Kaye, Adel K ElnaggarAbstract:Translocations and gene fusions have an important early role in tumorigenesis. The t(11;19) translocation and its CRTC1/MAML2 fusion transcript have been identified in several examples of both Warthin's tumor and Mucoepidermoid Carcinoma and are believed to be associated with the development of a subset of these tumors. To determine whether Warthin's tumor and Mucoepidermoid Carcinoma are genetically related, we used reverse transcriptase-polymerase chain reaction and DNA sequencing to analyze microdissected components of three tumors consisting of Warthin's tumor and Mucoepidermoid Carcinoma. We also investigated a metastatic melanoma to Warthin's tumor and a Warthin's Carcinoma of the parotid gland for comparison. The fusion transcript was identified in both Warthin's tumor and matching Mucoepidermoid Carcinoma components of all three tumors, in the Warthin's Carcinoma, and in the Warthin's tumor component but not in the metastatic melanoma. The results provide evidence for a link between the t(11;19) fusion gene and the development of a subset of Warthin's tumors with concurrent Mucoepidermoid Carcinoma and possible malignant transformation to Warthin's Carcinoma. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat. © 2008 Wiley-Liss, Inc.
Diana Bell - One of the best experts on this subject based on the ideXlab platform.
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primary intraosseous Mucoepidermoid Carcinoma of the jaw reappraisal of the md anderson cancer center experience
Head and Neck-journal for The Sciences and Specialties of The Head and Neck, 2016Co-Authors: Diana Bell, Carol M Lewis, Adel K Elnaggar, Randal S WeberAbstract:Background Mucoepidermoid Carcinoma arises from major or minor salivary glands, making up 10% of salivary gland tumors. Intraosseous Mucoepidermoid Carcinomas are rare, and make up only 2% to 3% of all Mucoepidermoid Carcinomas. The t(11;19) and its CRTC1-MAML2 fusion gene transcript have been identified in Mucoepidermoid Carcinoma and are associated with a subset of Mucoepidermoid Carcinomas. The extent to which the transcript influences disease features and patient survival is unclear. Methods We conducted a retrospective analysis of records for clinical features, surgical interventions, and prognoses. Reverse transcriptase-polymerase chain reaction (RT-PCR) and fluorescence in situ hybridization (FISH) used to assess the t(11;19) fusion gene in intraosseous Mucoepidermoid Carcinoma. Results Twenty-five patients with intraosseous Mucoepidermoid Carcinoma treated between 1998 and 2013 were identified. The t(11;19) fusion gene transcript CRTC1-MAML2 manifested in 9 intraosseous Mucoepidermoid Carcinomas, whereas is was not detected in another 9 intraosseous Carcinomas. Although the incidence of this fusion in Mucoepidermoid Carcinoma varies, it is generally accepted that more than 50% of this entity manifest the CRTC1-MAML2. Conclusion Intraosseous Mucoepidermoid Carcinoma diagnosis should be based on clinical and pathologic manifestations and complete resection is the first choice for patient treatment. The need for neck dissection and adjuvant treatment are debatable. Radiotherapy may improve prognosis and may be recommended in the postoperative period. Primary intraosseous Mucoepidermoid Carcinoma can manifest the fusion transcript in a subset of tumors. © 2015 Wiley Periodicals, Inc. Head Neck, 2015
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crtc1 maml2 fusion transcript in warthin s tumor and Mucoepidermoid Carcinoma evidence for a common genetic association
Genes Chromosomes and Cancer, 2008Co-Authors: Diana Bell, Randal S Weber, Mario A Luna, Frederic J Kaye, Adel K ElnaggarAbstract:Translocations and gene fusions have an important early role in tumorigenesis. The t(11;19) translocation and its CRTC1/MAML2 fusion transcript have been identified in several examples of both Warthin's tumor and Mucoepidermoid Carcinoma and are believed to be associated with the development of a subset of these tumors. To determine whether Warthin's tumor and Mucoepidermoid Carcinoma are genetically related, we used reverse transcriptase-polymerase chain reaction and DNA sequencing to analyze microdissected components of three tumors consisting of Warthin's tumor and Mucoepidermoid Carcinoma. We also investigated a metastatic melanoma to Warthin's tumor and a Warthin's Carcinoma of the parotid gland for comparison. The fusion transcript was identified in both Warthin's tumor and matching Mucoepidermoid Carcinoma components of all three tumors, in the Warthin's Carcinoma, and in the Warthin's tumor component but not in the metastatic melanoma. The results provide evidence for a link between the t(11;19) fusion gene and the development of a subset of Warthin's tumors with concurrent Mucoepidermoid Carcinoma and possible malignant transformation to Warthin's Carcinoma. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat.
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crtc1 maml2 fusion transcript in warthin s tumor and Mucoepidermoid Carcinoma evidence for a common genetic association
Genes Chromosomes and Cancer, 2008Co-Authors: Diana Bell, Randal S Weber, Mario A Luna, Frederic J Kaye, Adel K ElnaggarAbstract:Translocations and gene fusions have an important early role in tumorigenesis. The t(11;19) translocation and its CRTC1/MAML2 fusion transcript have been identified in several examples of both Warthin's tumor and Mucoepidermoid Carcinoma and are believed to be associated with the development of a subset of these tumors. To determine whether Warthin's tumor and Mucoepidermoid Carcinoma are genetically related, we used reverse transcriptase-polymerase chain reaction and DNA sequencing to analyze microdissected components of three tumors consisting of Warthin's tumor and Mucoepidermoid Carcinoma. We also investigated a metastatic melanoma to Warthin's tumor and a Warthin's Carcinoma of the parotid gland for comparison. The fusion transcript was identified in both Warthin's tumor and matching Mucoepidermoid Carcinoma components of all three tumors, in the Warthin's Carcinoma, and in the Warthin's tumor component but not in the metastatic melanoma. The results provide evidence for a link between the t(11;19) fusion gene and the development of a subset of Warthin's tumors with concurrent Mucoepidermoid Carcinoma and possible malignant transformation to Warthin's Carcinoma. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat. © 2008 Wiley-Liss, Inc.
Mario A Luna - One of the best experts on this subject based on the ideXlab platform.
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crtc1 maml2 fusion transcript in warthin s tumor and Mucoepidermoid Carcinoma evidence for a common genetic association
Genes Chromosomes and Cancer, 2008Co-Authors: Diana Bell, Randal S Weber, Mario A Luna, Frederic J Kaye, Adel K ElnaggarAbstract:Translocations and gene fusions have an important early role in tumorigenesis. The t(11;19) translocation and its CRTC1/MAML2 fusion transcript have been identified in several examples of both Warthin's tumor and Mucoepidermoid Carcinoma and are believed to be associated with the development of a subset of these tumors. To determine whether Warthin's tumor and Mucoepidermoid Carcinoma are genetically related, we used reverse transcriptase-polymerase chain reaction and DNA sequencing to analyze microdissected components of three tumors consisting of Warthin's tumor and Mucoepidermoid Carcinoma. We also investigated a metastatic melanoma to Warthin's tumor and a Warthin's Carcinoma of the parotid gland for comparison. The fusion transcript was identified in both Warthin's tumor and matching Mucoepidermoid Carcinoma components of all three tumors, in the Warthin's Carcinoma, and in the Warthin's tumor component but not in the metastatic melanoma. The results provide evidence for a link between the t(11;19) fusion gene and the development of a subset of Warthin's tumors with concurrent Mucoepidermoid Carcinoma and possible malignant transformation to Warthin's Carcinoma. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat.
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crtc1 maml2 fusion transcript in warthin s tumor and Mucoepidermoid Carcinoma evidence for a common genetic association
Genes Chromosomes and Cancer, 2008Co-Authors: Diana Bell, Randal S Weber, Mario A Luna, Frederic J Kaye, Adel K ElnaggarAbstract:Translocations and gene fusions have an important early role in tumorigenesis. The t(11;19) translocation and its CRTC1/MAML2 fusion transcript have been identified in several examples of both Warthin's tumor and Mucoepidermoid Carcinoma and are believed to be associated with the development of a subset of these tumors. To determine whether Warthin's tumor and Mucoepidermoid Carcinoma are genetically related, we used reverse transcriptase-polymerase chain reaction and DNA sequencing to analyze microdissected components of three tumors consisting of Warthin's tumor and Mucoepidermoid Carcinoma. We also investigated a metastatic melanoma to Warthin's tumor and a Warthin's Carcinoma of the parotid gland for comparison. The fusion transcript was identified in both Warthin's tumor and matching Mucoepidermoid Carcinoma components of all three tumors, in the Warthin's Carcinoma, and in the Warthin's tumor component but not in the metastatic melanoma. The results provide evidence for a link between the t(11;19) fusion gene and the development of a subset of Warthin's tumors with concurrent Mucoepidermoid Carcinoma and possible malignant transformation to Warthin's Carcinoma. This article contains Supplementary Material available at http://www.interscience.wiley.com/jpages/1045-2257/suppmat. © 2008 Wiley-Liss, Inc.
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salivary Mucoepidermoid Carcinoma revisited
Advances in Anatomic Pathology, 2006Co-Authors: Mario A LunaAbstract:Mucoepidermoid Carcinoma (MEC) is a malignant epithelial neoplasm composed of varying proportions of mucous, epidermoid, intermediate, columnar, and clear cells and often demonstrates prominent cystic growth. MEC is usually subclassified as low, intermediate, or high grade on the basis of its histologic features, including the presence of cystic spaces, cellular differentiation, proportion of mucous cells, growth pattern, type of invasion, and cytologic atypia. Because even low-grade neoplasms may metastasize, the term Mucoepidermoid tumor is inappropriate. The 3-level grading approach to tumor classification has found general acceptance among pathologists; differences in biologic behavior can be demonstrated even though clinical stage has become a better prognosticator. However, in the case of MEC, no universal agreement exists regarding which histologic grading criteria are most the useful, and grading has varied. These issues have led to the investigation of more subjective systems. We describe these new schemes, the histologic variants of MEC, and the ancillary methods that allow for further stratification of patients with MEC, especially for patients with grade 2 tumors, which have a variable and unpredictable clinical course.
Satoru Miyabe - One of the best experts on this subject based on the ideXlab platform.
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clinicopathological significance of the crtc3 maml2 fusion transcript in Mucoepidermoid Carcinoma
Modern Pathology, 2009Co-Authors: Takahisa Nakayama, Mitsukuni Okabe, Satoru Miyabe, Hitoshi Nagatsuka, Hidenori Sakuma, Kei Ijichi, Yasuhisa Hasegawa, Kazuo ShimozatoAbstract:Mucoepidermoid Carcinoma is the most common primary malignancy of the salivary gland. We and others showed that CRTC1-MAML2 gene fusion was associated with favorable clinicopathological tumor features. Recently, a novel gene fusion, CRTC3-MAML2, was reported as a rare gene alteration in a case of Mucoepidermoid Carcinoma. However, its frequency and clinicopathological significance remains unclear. In all, 101 cases of Mucoepidermoid Carcinoma and 89 cases of non-Mucoepidermoid Carcinoma of the salivary gland were analyzed, and RNA was extracted from formalin-fixed, paraffin-embedded specimens. In the CRTC family, there have been three genes, CRTC1, CRTC2, and CRTC3. We developed reverse transcription-polymerase chain reaction (RT-PCR) assays for CRTC1-MAML2, CRTC2-MAML2, and CRTC3-MAML2 fusions. Clinicopathological data of the patients were obtained from their clinical records. Of 101 cases of Mucoepidermoid Carcinoma, 34 (34%) and 6 (6%) were positive for CRTC1-MAML2 and CRTC3-MAML2 fusion transcripts. However, in the 89 cases of non-Mucoepidermoid Carcinoma, neither transcript was noted. In the former cases, CRTC1-MAML2 and CRTC3-MAML2 fusions were mutually exclusive. The other fusion, CRTC2-MAML2, was not detected. We confirmed that the clinicopathological features of CRTC1-MAML2-positive Mucoepidermoid Carcinomas indicated an indolent course. CRTC3-MAML2-positive Mucoepidermoid Carcinomas also had clinicopathologically favorable features; all cases showed a less advanced clinical stage, negative nodal metastasis, no high-grade tumor histology, and no recurrence or tumor-related death after surgical resection of the tumor. It is interesting to note that patients with CRTC3-MAML2-positive tumors (mean 36 years of age) were significantly younger that those with the CRTC1-MAML2 fusion (55 years) and those with fusion-negative tumors (58 years). In conclusion, CRTC3-MAML2 fusion, which is mutually exclusive with CRTC1-MAML2 fusion and specific to Mucoepidermoid Carcinoma, may be detected more frequently than previously expected. Mucoepidermoid Carcinomas possessing CRTC3-MAML2 fusion may be associated with favorable clinicopathological features and patients may be younger than those with CRTC1-MAML2 fusion or those with no detectable gene fusion.
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mect1 maml2 fusion transcript defines a favorable subset of Mucoepidermoid Carcinoma
Clinical Cancer Research, 2006Co-Authors: Mitsukuni Okabe, Satoru Miyabe, Hitoshi Nagatsuka, Akihiro Terada, Nobuhiro Hanai, Motoo Yokoi, Kazuo Shimozato, Tadaaki Eimoto, Shigeo NakamuraAbstract:Purpose: Mucoepidermoid Carcinoma is the most common primary malignancy of the salivary gland. Mucoepidermoid Carcinoma translocated gene 1-mastermind-like gene family ( MECT1-MAML2 ) gene fusion was identified from a recurring t(11;19)(q21;p13) translocation, which is often the sole cytogenetic alteration in this disease. This fusion transcript has been frequently detected in Mucoepidermoid Carcinoma and shown to be involved in the transformation of epithelial cells. However, its clinicopathologic significance remains unclear. Experimental Design: Seventy-one cases of Mucoepidermoid Carcinoma and 51 cases of nonMucoepidermoid Carcinoma salivary gland tumors (including 26 Warthin tumor cases) were retrospectively analyzed. RNA was extracted from archival materials: histologic paraffin specimens in all cases and cytologic specimens in 10 Mucoepidermoid Carcinoma cases. The MECT1-MAML2 fusion transcript was detected by a reverse transcription-PCR assay, which can be applied to both histologic and cytologic specimens. The presence of the fusion transcript was correlated with relevant clinicopathologic and survival data of the Mucoepidermoid Carcinoma patients. Results: The MECT1-MAML2 fusion transcript was detected in 27 of the 71 (38%) Mucoepidermoid Carcinoma cases but not in any case of nonMucoepidermoid Carcinoma tumors. The reverse transcription-PCR results showed no difference between histologic and cytologic specimens. Detection of the MECT1-MAML2 fusion transcript was associated with a less advanced clinical stage and a low-grade tumor histology. The presence of the transcript was associated with longer disease-free and overall survivals on univariate analysis and emerged as an independent prognostic factor for longer overall survival on multivariate analysis. Conclusions: The MECT1-MAML2 fusion transcript may be specific to Mucoepidermoid Carcinoma and associated with a distinct Mucoepidermoid Carcinoma subset that exhibits favorable clinicopathologic features and an indolent clinical course.
