The Experts below are selected from a list of 132 Experts worldwide ranked by ideXlab platform
Iyadh Douagi - One of the best experts on this subject based on the ideXlab platform.
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Characterization of Stem-Like Cells in Mucoepidermoid Tracheal Paediatric Tumor
PloS one, 2014Co-Authors: Mei Ling Lim, Brandon Nick Sern Ooi, Philipp Jungebluth, Sebastian Sjöqvist, Isabell Hultman, Greg Lemon, Ylva Gustafsson, Jurate Asmundsson, Silvia Baiguera, Iyadh DouagiAbstract:Stem cells contribute to regeneration of tissues and organs. Cells with stem cell-like properties have been identified in Tumors from a variety of origins, but to our knowledge there are yet no reports on Tumor-related stem cells in the human upper respiratory tract. In the present study, we show that a tracheal Mucoepidermoid Tumor biopsy obtained from a 6 year-old patient contained a subpopulation of cells with morphology, clonogenicity and surface markers that overlapped with bone marrow mesenchymal stromal cells (BM-MSCs). These cells, designated as MEi (mesenchymal stem cell-like Mucoepidermoid Tumor) cells, could be differentiated towards mesenchymal lineages both with and without induction, and formed spheroids in vitro. The MEi cells shared several multipotent characteristics with BM-MSCs. However, they displayed differences to BM-MSCs in growth kinectics and gene expression profiles relating to cancer pathways and tube development. Despite this, the MEi cells did not possess in vivo Tumor-initiating capacity, as proven by the absence of growth in situ after localized injection in immunocompromised mice. Our results provide an initial characterization of benign tracheal cancer-derived niche cells. We believe that this report could be of importance to further understand tracheal cancer initiation and progression as well as therapeutic development.
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Histological and pathological analyses of a Mucoepidermoid Tumor.
2014Co-Authors: Mei Ling Lim, Brandon Nick Sern Ooi, Philipp Jungebluth, Sebastian Sjöqvist, Isabell Hultman, Greg Lemon, Ylva Gustafsson, Jurate Asmundsson, Silvia Baiguera, Iyadh DouagiAbstract:Haematoxylin and Eosin staining provides visualization of the excised tracheal Mucoepidermoid Tumor biopsy (a), periodic acid-Schiff stain identifies mucins and glycoproteins in magenta (b), Mucin 1, Muc-1 identifies mucin-producing cells (c), Cytokeratin marker, Ck-MNF116 shows epithelial staining (d), carcino-embryonic antigen, CEA is an oncofetal antigen expressed by some Tumors but not in normal adult tissues (e), androgen receptor, AR is a predictive marker for choice of therapy (f), muscle specific actin, MSA identifies the stromal component (g), and Ki-67 is a cell proliferation marker used for quantifying the proliferative index of the Tumor (h). Scale bar: 100 µm.
Mei Ling Lim - One of the best experts on this subject based on the ideXlab platform.
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Characterization of Stem-Like Cells in Mucoepidermoid Tracheal Paediatric Tumor
PloS one, 2014Co-Authors: Mei Ling Lim, Brandon Nick Sern Ooi, Philipp Jungebluth, Sebastian Sjöqvist, Isabell Hultman, Greg Lemon, Ylva Gustafsson, Jurate Asmundsson, Silvia Baiguera, Iyadh DouagiAbstract:Stem cells contribute to regeneration of tissues and organs. Cells with stem cell-like properties have been identified in Tumors from a variety of origins, but to our knowledge there are yet no reports on Tumor-related stem cells in the human upper respiratory tract. In the present study, we show that a tracheal Mucoepidermoid Tumor biopsy obtained from a 6 year-old patient contained a subpopulation of cells with morphology, clonogenicity and surface markers that overlapped with bone marrow mesenchymal stromal cells (BM-MSCs). These cells, designated as MEi (mesenchymal stem cell-like Mucoepidermoid Tumor) cells, could be differentiated towards mesenchymal lineages both with and without induction, and formed spheroids in vitro. The MEi cells shared several multipotent characteristics with BM-MSCs. However, they displayed differences to BM-MSCs in growth kinectics and gene expression profiles relating to cancer pathways and tube development. Despite this, the MEi cells did not possess in vivo Tumor-initiating capacity, as proven by the absence of growth in situ after localized injection in immunocompromised mice. Our results provide an initial characterization of benign tracheal cancer-derived niche cells. We believe that this report could be of importance to further understand tracheal cancer initiation and progression as well as therapeutic development.
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Histological and pathological analyses of a Mucoepidermoid Tumor.
2014Co-Authors: Mei Ling Lim, Brandon Nick Sern Ooi, Philipp Jungebluth, Sebastian Sjöqvist, Isabell Hultman, Greg Lemon, Ylva Gustafsson, Jurate Asmundsson, Silvia Baiguera, Iyadh DouagiAbstract:Haematoxylin and Eosin staining provides visualization of the excised tracheal Mucoepidermoid Tumor biopsy (a), periodic acid-Schiff stain identifies mucins and glycoproteins in magenta (b), Mucin 1, Muc-1 identifies mucin-producing cells (c), Cytokeratin marker, Ck-MNF116 shows epithelial staining (d), carcino-embryonic antigen, CEA is an oncofetal antigen expressed by some Tumors but not in normal adult tissues (e), androgen receptor, AR is a predictive marker for choice of therapy (f), muscle specific actin, MSA identifies the stromal component (g), and Ki-67 is a cell proliferation marker used for quantifying the proliferative index of the Tumor (h). Scale bar: 100 µm.
William H Westra - One of the best experts on this subject based on the ideXlab platform.
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Mucoepidermoid carcinoma of the oropharynx a Tumor type with a propensity for regional metastasis unrelated to histologic grade
Human Pathology, 2019Co-Authors: Pooja Navale, Lisa M Rooper, Justin A Bishop, William H WestraAbstract:Summary The designation “Mucoepidermoid Tumor” is a historic one used in reference to a form of Mucoepidermoid carcinoma (MEC) that was believed to be benign. This bygone notion was based on the observation that the vast majority of MECs arising from the intraoral minor salivary glands behave in a benign fashion, particularly when they do not exhibit high grade features. There has been a recent move to partition the oral vault into the oral cavity proper and the oropharynx based on an awareness that these compartments are distinct, and that similar Tumor types arising from these compartments may behave in dramatically different ways (e.g. oral cavity squamous cell carcinoma vs. oropharyngeal squamous cell carcinoma). Design The pathology data bases from three large academic medical centers were searched for cases of MECs arising in the oropharynx. Relevant clinical and pathological information was collected from the medical records. Twenty-five cases were identified. They were from 18 females (72%) and 7 males (28%), ranging in age from 31 to 88 years (median, 61). Twenty-two (88%) were classified as low (n = 12) or intermediate (n = 10) grade, and three (12%) as high grade. Most arose from the base of tongue (n = 24), but one arose from the lateral pharyngeal wall. The median Tumor size was 2.0 cm. Nineteen patients underwent neck node dissections. Of these, 13 (68%) had histologically documented lymph node metastases. MECs that lacked high grade features were almost as likely to metastasize as those with high grade features (50% vs 66%, Fischer exact = 1). Of three metastases tested, two harbored the MAML2 gene fusion. MECs arising from the base of tongue are associated with an alarmingly high rate of nodal metastases. This behavior cannot be predicted by histologic grading or MAML2 status. The propensity to metastasize may to some degree reflect the unique microenvironment of the oropharynx.
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Mucoepidermoid carcinoma of the oropharynx: a Tumor type with a propensity for regional metastasis unrelated to histologic grade.
Human pathology, 2019Co-Authors: Pooja Navale, Lisa M Rooper, Justin A Bishop, William H WestraAbstract:Summary The designation “Mucoepidermoid Tumor” is a historic one used in reference to a form of Mucoepidermoid carcinoma (MEC) that was believed to be benign. This bygone notion was based on the observation that the vast majority of MECs arising from the intraoral minor salivary glands behave in a benign fashion, particularly when they do not exhibit high grade features. There has been a recent move to partition the oral vault into the oral cavity proper and the oropharynx based on awareness that these compartments are distinct, and that similar Tumor types arising from these compartments may behave in dramatically different ways (e.g, oral cavity squamous cell carcinoma vs oropharyngeal squamous cell carcinoma). The pathology databases from 3 large academic medical centers were searched for cases of MECs arising in the oropharynx. Relevant clinical and pathological information was collected from the medical records. Twenty-five cases were identified. They were from 18 females (72%) and 7 males (28%), ranging in age from 31 to 88 years (median, 61). Twenty-two (88%) were classified as low (n = 12) or intermediate (n = 10) grade, and 3 (12%) as high grade. Most arose from the base of tongue (n = 24), but one arose from the lateral pharyngeal wall. The median Tumor size was 2.0 cm. Nineteen patients underwent neck node dissections. Of these, 13 (68%) had histologically documented lymph node metastases. MECs that lacked high grade features were almost as likely to metastasize as those with high grade features (50% vs 66%, Fisher exact = 1). Of 3 metastases tested, 2 harbored the MAML2 gene fusion. MECs arising from the base of tongue are associated with an alarmingly high rate of nodal metastases. This behavior cannot be predicted by histologic grading or MAML2 status. The propensity to metastasize may to some degree reflect the unique microenvironment of the oropharynx.
Eric N. Mendeloff - One of the best experts on this subject based on the ideXlab platform.
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Tracheal Mucoepidermoid carcinoma in a 7-year-old child
The Annals of thoracic surgery, 1998Co-Authors: Seiichi Noda, Sudhir Sundaresan, Eric N. MendeloffAbstract:Mucoepidermoid cancers are rare Tumors that arise from the serous and mucous glands of the upper airway and salivary glands. Patients, especially children, with Tumors that arise in the trachea and upper airways are often misdiagnosed as asthmatic and are treated with bronchodilators without resolution. A 7-year-old girl who had been diagnosed as asthmatic was subsequently found to have a Mucoepidermoid Tumor of the trachea. She underwent a successful tracheal resection and remains Tumor free on follow-up bronchoscopy at 16 months.
Sebastian Sjöqvist - One of the best experts on this subject based on the ideXlab platform.
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Characterization of Stem-Like Cells in Mucoepidermoid Tracheal Paediatric Tumor
PloS one, 2014Co-Authors: Mei Ling Lim, Brandon Nick Sern Ooi, Philipp Jungebluth, Sebastian Sjöqvist, Isabell Hultman, Greg Lemon, Ylva Gustafsson, Jurate Asmundsson, Silvia Baiguera, Iyadh DouagiAbstract:Stem cells contribute to regeneration of tissues and organs. Cells with stem cell-like properties have been identified in Tumors from a variety of origins, but to our knowledge there are yet no reports on Tumor-related stem cells in the human upper respiratory tract. In the present study, we show that a tracheal Mucoepidermoid Tumor biopsy obtained from a 6 year-old patient contained a subpopulation of cells with morphology, clonogenicity and surface markers that overlapped with bone marrow mesenchymal stromal cells (BM-MSCs). These cells, designated as MEi (mesenchymal stem cell-like Mucoepidermoid Tumor) cells, could be differentiated towards mesenchymal lineages both with and without induction, and formed spheroids in vitro. The MEi cells shared several multipotent characteristics with BM-MSCs. However, they displayed differences to BM-MSCs in growth kinectics and gene expression profiles relating to cancer pathways and tube development. Despite this, the MEi cells did not possess in vivo Tumor-initiating capacity, as proven by the absence of growth in situ after localized injection in immunocompromised mice. Our results provide an initial characterization of benign tracheal cancer-derived niche cells. We believe that this report could be of importance to further understand tracheal cancer initiation and progression as well as therapeutic development.
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Histological and pathological analyses of a Mucoepidermoid Tumor.
2014Co-Authors: Mei Ling Lim, Brandon Nick Sern Ooi, Philipp Jungebluth, Sebastian Sjöqvist, Isabell Hultman, Greg Lemon, Ylva Gustafsson, Jurate Asmundsson, Silvia Baiguera, Iyadh DouagiAbstract:Haematoxylin and Eosin staining provides visualization of the excised tracheal Mucoepidermoid Tumor biopsy (a), periodic acid-Schiff stain identifies mucins and glycoproteins in magenta (b), Mucin 1, Muc-1 identifies mucin-producing cells (c), Cytokeratin marker, Ck-MNF116 shows epithelial staining (d), carcino-embryonic antigen, CEA is an oncofetal antigen expressed by some Tumors but not in normal adult tissues (e), androgen receptor, AR is a predictive marker for choice of therapy (f), muscle specific actin, MSA identifies the stromal component (g), and Ki-67 is a cell proliferation marker used for quantifying the proliferative index of the Tumor (h). Scale bar: 100 µm.