The Experts below are selected from a list of 2028 Experts worldwide ranked by ideXlab platform
Yuanda Song - One of the best experts on this subject based on the ideXlab platform.
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role of g6pdh and leub on lipid accumulation in Mucor circinelloides
Journal of Agricultural and Food Chemistry, 2020Co-Authors: Xin Tang, Haiqin Chen, Hao Zhang, Wei Chen, Yuanda Song, Yong Q. ChenAbstract:Mucor circinelloides is a valuable oleaginous filamentous fungus rich in γ-linolenic acid (GLA, 18:3; n-6), which is beneficial for human health. Our previous comparative proteomic analysis between...
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Antioxidant activity of polyphenolic extracts of filamentous fungus Mucor circinelloides (WJ11): Extraction, characterization and storage stability of food emulsions
Food Bioscience, 2020Co-Authors: Ahsan Hameed, Syed Ammar Hussain, Muhammad Umair Ijaz, Samee Ullah, Zafarullah Muhammad, Hafiz Ansar Rasul Suleria, Yuanda SongAbstract:Optimized extraction of antioxidants from Mucor circinelloides WJ11 (MCWJ11) biomass was studied. Storage, stability, composition and antioxidant properties in model food emulsion systems were also investigated. Both the extraction approach and solvent type were found to significantly (p
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Improved SDA Production in High Lipid Accumulating Strain of Mucor circinelloides WJ11 by Genetic Modification
American Journal of Biochemistry and Biotechnology, 2020Co-Authors: Junhuan Yang, Victoriano Garre, Huaiyuan Zhang, Ahsanul Kabir Khan, Sergio López-garcía, Shaista Nosheen, Yusuf Nazir, Yuanda SongAbstract:Stearidonic Acid (SDA; 18:4, n-3) is a ω-3 polyunsaturated fatty acid which is nutritionally important and has pharmaceutical applications. Hence, scientists are trying to construct SDA producing oleaginous microorganisms by genetic modification. Two enzymes, Delta-6 Desaturase (D6D) and ω-3 desaturase catalyze the reactions to produce SDA from Linoleic Acid (LA; 18:2, n-6). But the key enzyme, ω-3 desaturase converts LA to ALA and GLA to SDA is absent in high lipid accumulating Mucor circinelloides WJ11, retarding its ability to produce SDA. Therefore, in this study, we overexpressed fad3 gene from Arabidopsis thaliana in M. circinelloides WJ11 to produce SDA. Overexpression of fad3 gene in M. circinelloides WJ11 resulted in the production of 340 mg/l SDA. This research opened a new opportunity to make use of this fungus for industrial production of SDA.
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Construction of DGLA producing cell factory by genetic modification of Mucor circinelloides.
Microbial cell factories, 2019Co-Authors: Ahsanul Kabir Khan, Syed Ammar Hussain, Victoriano Garre, Junhuan Yang, Huaiyuan Zhang, Li Liang, Yuanda SongAbstract:Dihomo-gamma linolenic acid (DGLA, 20:3, n-6) is the elongated product of Gamma linolenic acid (GLA, 18:3, n-6) catalyzed by the enzyme delta-6 elongase (D6E) or gamma linolenic acid elongase (GLELO). Construction of engineered oleaginous microbes have been attracting significant interest to produce DGLA because of its nutritional value and medicinal applications. Mucor circinelloides is a GLA producing filamentous fungus which can be a useful tool to produce DGLA. We have, therefore, overexpressed the D6E (GLELO) gene in this fungus to construct DGLA producing cell factory. To produce DGLA in M. circinelloides, homologous overexpression of D6E (GLELO) gene was analyzed. When the gene was overexpressed in M. circinelloides CBS277.49, up to 5.72% DGLA was produced in this strain. To our knowledge, this is the first report describing the overexpression of D6E (GLELO) gene in M. circinelloides to construct DGLA producing cell factory. A new scope for further research has been established by this work for improved production of DGLA in this fungus, specifically in its high lipid-producing strain, WJ11.
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increased lipid accumulation in Mucor circinelloides by overexpression of mitochondrial citrate transporter genes
Industrial & Engineering Chemistry Research, 2019Co-Authors: Junhuan Yang, Md. Ahsanul Kabir Khan, Victoriano Garre, Wanwipa Vongsangnak, Yuanda SongAbstract:Mucor circinelloides has been commonly used as the model microbe to investigate lipid production as an oleaginous fungus. Mitochondrial citrate transporter can catalyze the translocation of the cit...
Victoriano Garre - One of the best experts on this subject based on the ideXlab platform.
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Improved SDA Production in High Lipid Accumulating Strain of Mucor circinelloides WJ11 by Genetic Modification
American Journal of Biochemistry and Biotechnology, 2020Co-Authors: Junhuan Yang, Victoriano Garre, Huaiyuan Zhang, Ahsanul Kabir Khan, Sergio López-garcía, Shaista Nosheen, Yusuf Nazir, Yuanda SongAbstract:Stearidonic Acid (SDA; 18:4, n-3) is a ω-3 polyunsaturated fatty acid which is nutritionally important and has pharmaceutical applications. Hence, scientists are trying to construct SDA producing oleaginous microorganisms by genetic modification. Two enzymes, Delta-6 Desaturase (D6D) and ω-3 desaturase catalyze the reactions to produce SDA from Linoleic Acid (LA; 18:2, n-6). But the key enzyme, ω-3 desaturase converts LA to ALA and GLA to SDA is absent in high lipid accumulating Mucor circinelloides WJ11, retarding its ability to produce SDA. Therefore, in this study, we overexpressed fad3 gene from Arabidopsis thaliana in M. circinelloides WJ11 to produce SDA. Overexpression of fad3 gene in M. circinelloides WJ11 resulted in the production of 340 mg/l SDA. This research opened a new opportunity to make use of this fungus for industrial production of SDA.
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Construction of DGLA producing cell factory by genetic modification of Mucor circinelloides.
Microbial cell factories, 2019Co-Authors: Ahsanul Kabir Khan, Syed Ammar Hussain, Victoriano Garre, Junhuan Yang, Huaiyuan Zhang, Li Liang, Yuanda SongAbstract:Dihomo-gamma linolenic acid (DGLA, 20:3, n-6) is the elongated product of Gamma linolenic acid (GLA, 18:3, n-6) catalyzed by the enzyme delta-6 elongase (D6E) or gamma linolenic acid elongase (GLELO). Construction of engineered oleaginous microbes have been attracting significant interest to produce DGLA because of its nutritional value and medicinal applications. Mucor circinelloides is a GLA producing filamentous fungus which can be a useful tool to produce DGLA. We have, therefore, overexpressed the D6E (GLELO) gene in this fungus to construct DGLA producing cell factory. To produce DGLA in M. circinelloides, homologous overexpression of D6E (GLELO) gene was analyzed. When the gene was overexpressed in M. circinelloides CBS277.49, up to 5.72% DGLA was produced in this strain. To our knowledge, this is the first report describing the overexpression of D6E (GLELO) gene in M. circinelloides to construct DGLA producing cell factory. A new scope for further research has been established by this work for improved production of DGLA in this fungus, specifically in its high lipid-producing strain, WJ11.
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Mucor circinelloides thrives inside the phagosome through an atf mediated germination pathway
Mbio, 2019Co-Authors: Carlos Perezarques, Joseph Heitman, Maria Isabel Navarromendoza, Laura Murcia, Francisco E Nicolas, Carlos Lax, Pablo Martinezgarcia, Victoriano GarreAbstract:ABSTRACT Mucormycosis is an emerging fungal infection that is often lethal due to the ineffectiveness of current therapies. Here, we have studied the first stage of this infection—the germination of Mucor circinelloides spores inside phagocytic cells—from an integrated transcriptomic and functional perspective. A relevant fungal gene network is remodeled in response to phagocytosis, being enriched in crucial functions to survive and germinate inside the phagosome, such as nutritional adaptation and response to oxidative stress. Correspondingly, the phagocytic cells induced a specific proinflammatory and apoptotic response to the pathogenic strain. Deletion of fungal genes encoding putative transcription factors (atf1, atf2, and gcn4), extracellular proteins (chi1 and pps1), and an aquaporin (aqp1) revealed that these genes perform important roles in survival following phagocytosis, germination inside the phagosome, and virulence in mice. atf1 and atf2 play a major role in these pathogenic processes, since their mutants showed the strongest phenotypes and both genes control a complex gene network of secondarily regulated genes, including chi1 and aqp1. These new insights into the initial phase of Mucormycosis define genetic regulators and molecular processes that could serve as pharmacological targets. IMPORTANCE Mucorales are a group of ancient saprophytic fungi that cause neglected infectious diseases collectively known as Mucormycoses. The molecular processes underlying the establishment and progression of this disease are largely unknown. Our work presents a transcriptomic study to unveil the Mucor circinelloides genetic network triggered in fungal spores in response to phagocytosis by macrophages and the transcriptional response of the host cells. Functional characterization of differentially expressed fungal genes revealed three transcription factors and three extracellular proteins essential for the fungus to survive and germinate inside the phagosome and to cause disease in mice. Two of the transcription factors, highly similar to activating transcription factors (ATFs), coordinate a complex secondary gene response involved in pathogenesis. The significance of our research is in characterizing the initial stages that lead to evasion of the host innate immune response and, in consequence, the dissemination of the infection. This genetic study offers possible targets for novel antifungal drugs against these opportunistic human pathogens.
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increased lipid accumulation in Mucor circinelloides by overexpression of mitochondrial citrate transporter genes
Industrial & Engineering Chemistry Research, 2019Co-Authors: Junhuan Yang, Md. Ahsanul Kabir Khan, Victoriano Garre, Wanwipa Vongsangnak, Yuanda SongAbstract:Mucor circinelloides has been commonly used as the model microbe to investigate lipid production as an oleaginous fungus. Mitochondrial citrate transporter can catalyze the translocation of the cit...
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Construction of DGLA producing cell factory by genetic modification of Mucor circinelloides
BMC, 2019Co-Authors: Md. Ahsanul Kabir Khan, Syed Ammar Hussain, Victoriano Garre, Junhuan Yang, Huaiyuan Zhang, Li Liang, Yuanda SongAbstract:Abstract Background Dihomo-gamma linolenic acid (DGLA, 20:3, n-6) is the elongated product of Gamma linolenic acid (GLA, 18:3, n-6) catalyzed by the enzyme delta-6 elongase (D6E) or gamma linolenic acid elongase (GLELO). Construction of engineered oleaginous microbes have been attracting significant interest to produce DGLA because of its nutritional value and medicinal applications. Mucor circinelloides is a GLA producing filamentous fungus which can be a useful tool to produce DGLA. We have, therefore, overexpressed the D6E (GLELO) gene in this fungus to construct DGLA producing cell factory. Result To produce DGLA in M. circinelloides, homologous overexpression of D6E (GLELO) gene was analyzed. When the gene was overexpressed in M. circinelloides CBS277.49, up to 5.72% DGLA was produced in this strain. Conclusion To our knowledge, this is the first report describing the overexpression of D6E (GLELO) gene in M. circinelloides to construct DGLA producing cell factory. A new scope for further research has been established by this work for improved production of DGLA in this fungus, specifically in its high lipid-producing strain, WJ11
Tamás Papp - One of the best experts on this subject based on the ideXlab platform.
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Development of a plasmid free CRISPR-Cas9 system for the genetic modification of Mucor circinelloides
Scientific Reports, 2017Co-Authors: Gábor Nagy, Csaba Vágvölgyi, Árpád Csernetics, Csilla Szebenyi, Amanda Grace Vaz, Eszter Judit Tóth, Tamás PappAbstract:Mucor circinelloides and other members of Mucorales are filamentous fungi, widely used as model organisms in basic and applied studies. Although genetic manipulation methods have been described for some Mucoral fungi, construction of stable integrative transformants by homologous recombination has remained a great challenge in these organisms. In the present study, a plasmid free CRISPR-Cas9 system was firstly developed for the genetic modification of a Mucoral fungus. The described method offers a rapid but robust tool to obtain mitotically stable mutants of M . circinelloides via targeted integration of the desired DNA. It does not require plasmid construction and its expression in the recipient organism. Instead, it involves the direct introduction of the guide RNA and the Cas9 enzyme and, in case of homology directed repair (HDR), the template DNA into the recipient strain. Efficiency of the method for non-homologous end joining (NHEJ) and HDR was tested by disrupting two different genes, i.e. carB encoding phytoene dehydrogenase and hmgR2 encoding 3-hydroxy-3-methylglutaryl-CoA reductase, of M . circinelloides . Both NHEJ and HDR resulted in stable gene disruption mutants. While NHEJ caused extensive deletions upstream from the protospacer adjacent motif, HDR assured the integration of the deletion cassette at the targeted site.
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Transcription of the three HMG-CoA reductase genes of Mucor circinelloides.
BMC microbiology, 2014Co-Authors: Gábor P. Nagy, Csaba Vágvölgyi, Anita Farkas, Árpád Csernetics, Ottó Bencsik, András Szekeres, Ildikó Nyilasi, Tamás PappAbstract:Background Precursors of sterols, carotenoids, the prenyl groups of several proteins and other terpenoid compounds are synthesised via the acetate-mevalonate pathway. One of the key enzyme of this pathway is the 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, which catalyses the conversion of HMG-CoA to mevalonate. HMG-CoA reductase therefore affects many biological processes, such as morphogenesis, synthesis of different metabolites or adaptation to environmental changes. In this study, transcription of the three HMG-CoA reductase genes (designated as hmgR1, hmgR2 and hmgR3) of the β-carotene producing Mucor circinelloides has been analysed under various culturing conditions; effect of the elevation of their copy number on the carotenoid and ergosterol content as well as on the sensitivity to statins has also been examined.
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Transcription of the three HMG-CoA reductase genes of Mucor circinelloides
BMC Microbiology, 2014Co-Authors: Gábor Nagy, Csaba Vágvölgyi, Anita Farkas, Árpád Csernetics, Ottó Bencsik, András Szekeres, Ildikó Nyilasi, Tamás PappAbstract:Background Precursors of sterols, carotenoids, the prenyl groups of several proteins and other terpenoid compounds are synthesised via the acetate-mevalonate pathway. One of the key enzyme of this pathway is the 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, which catalyses the conversion of HMG-CoA to mevalonate. HMG-CoA reductase therefore affects many biological processes, such as morphogenesis, synthesis of different metabolites or adaptation to environmental changes. In this study, transcription of the three HMG-CoA reductase genes (designated as hmgR1 , hmgR2 and hmgR3 ) of the β-carotene producing Mucor circinelloides has been analysed under various culturing conditions; effect of the elevation of their copy number on the carotenoid and ergosterol content as well as on the sensitivity to statins has also been examined. Results Transcripts of each gene were detected and their relative levels varied under the tested conditions. Transcripts of hmgR1 were detected only in the mycelium and its relative transcript level seems to be strongly controlled by the temperature and the oxygen level of the environment. Transcripts of hmgR2 and hmgR3 are already present in the germinating spores and the latter is also strongly regulated by oxygen. Overexpression of hmgR2 and hmgR3 by elevating their copy numbers increased the carotenoid content of the fungus and decreased their sensitivity to statins. Conclusions The three HMG-CoA reductase genes of M. circinelloides displayed different relative transcript levels under the tested conditions suggesting differences in their regulation. They seem to be especially involved in the adaptation to the changing oxygen tension and osmotic conditions of the environment as well as to statin treatment. Overexpression of hmgR2 and hmgR3 may be used to improve the carotenoid content.
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Canthaxanthin production with modified Mucor circinelloides strains
Applied Microbiology and Biotechnology, 2013Co-Authors: Tamás Papp, Árpád Csernetics, Ottó Bencsik, Arturo P Eslava, Enrique A Iturriaga, Gábor Nagy, Csaba VágvölgyiAbstract:Canthaxanthin is a natural diketo derivative of β-carotene primarily used by the food and feed industries. Mucor circinelloides is a β-carotene-accumulating zygomycete fungus and one of the model organisms to study the carotenoid biosynthesis in fungi. In this study, the β-carotene ketolase gene ( crtW ) of the marine bacterium Paracoccus sp. N81106 fused with fungal promoter and terminator regions was integrated into the M . circinelloides genome to construct stable canthaxanthin-producing strains. Different transformation methods including polyethylene glycol-mediated transformation with linear DNA fragments, restriction enzyme-mediated integration and Agrobacterium tumefaciens -mediated transformation were tested to integrate the crtW gene into the Mucor genome. Mitotic stability, site of integration and copy number of the transferred genes were analysed in the transformants, and several stable strains containing the crtW gene in high copy number were isolated. Carotenoid composition of selected transformants and effect of culturing conditions, such as temperature, carbon sources and application of certain additives in the culturing media, on their carotenoid content were analysed. Canthaxanthin-producing transformants were able to survive at higher growth temperature than the untransformed strain, maybe due to the effect of canthaxanthin on the membrane fluidity and integrity. With the application of glucose, trehalose, dihydroxyacetone and l -aspartic acid as sole carbon sources in minimal medium, the crtW -expressing M . circinelloides strain, MS12+pCA8lf/1, produced more than 200 μg/g (dry mass) of canthaxanthin.
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expression of three isoprenoid biosynthesis genes and their effects on the carotenoid production of the zygomycete Mucor circinelloides
Fungal Genetics and Biology, 2011Co-Authors: Árpád Csernetics, Csaba Vágvölgyi, Gábor P. Nagy, András Szekeres, Arturo P Eslava, Enrique A Iturriaga, Tamás PappAbstract:The zygomycete Mucor circinelloides accumulates β-carotene as the main carotenoid compound. In this study, the applicability of some early genes of the general isoprenoid pathway to improve the carotenoid production in this fungus was examined. The isopentenyl pyrophosphate isomerase gene (ipi) was cloned and used together with the genes encoding farnesyl pyrophosphate synthase (isoA) and geranylgeranyl pyrophosphate synthase (carG) in overexpression studies. Transformation experiments showed that the first bottleneck in the pathway, from the aspect of carotenoid production, is the step controlled by the carG gene, but overexpression of the ipi and isoA genes also contributes to the availability of the precursors. Transformations with these isoprenoid genes in combination with a bacterial β-carotene ketolase gene yielded Mucor strains producing canthaxanthin and echinenone.
Wei Chen - One of the best experts on this subject based on the ideXlab platform.
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role of g6pdh and leub on lipid accumulation in Mucor circinelloides
Journal of Agricultural and Food Chemistry, 2020Co-Authors: Xin Tang, Haiqin Chen, Hao Zhang, Wei Chen, Yuanda Song, Yong Q. ChenAbstract:Mucor circinelloides is a valuable oleaginous filamentous fungus rich in γ-linolenic acid (GLA, 18:3; n-6), which is beneficial for human health. Our previous comparative proteomic analysis between...
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Comparative Proteome Analysis between High Lipid-Producing Strain Mucor circinelloides WJ11 and Low Lipid-Producing Strain CBS 277.49
Journal of agricultural and food chemistry, 2017Co-Authors: Xin Tang, Haiqin Chen, Hao Zhang, Yuanda Song, Yong Q. Chen, Wei ChenAbstract:Mucor circinelloides is one of few oleaginous fungi that produces a useful oil rich in γ-linolenic acid, but it usually only produces
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comparative proteome analysis between high lipid producing strain Mucor circinelloides wj11 and low lipid producing strain cbs 277 49
Journal of Agricultural and Food Chemistry, 2017Co-Authors: Xin Tang, Haiqin Chen, Hao Zhang, Yuanda Song, Yong Q. Chen, Wei ChenAbstract:Mucor circinelloides is one of few oleaginous fungi that produces a useful oil rich in γ-linolenic acid, but it usually only produces <25% total lipid. Nevertheless, we isolated a new strain WJ11 that can produce up to 36% lipid of cell dry weight. In this study, we have systematically analyzed the global changes in protein levels between the high lipid-producing strain WJ11 and the low lipid-producing strain CBS 277.49 (15%, lipid/cell dry weight) at lipid accumulation phase through comparative proteome analysis. Proteome analysis demonstrated that the branched-chain amino acid and lysine metabolism, glycolytic pathway, and pentose phosphate pathway in WJ11 were up-regulated, while the activities of tricarboxylic acid cycle and branch point enzyme for synthesis of isoprenoids were retarded compared with CBS 277.49. The coordinated regulation at proteome level indicate that more acetyl-CoA and NADPH are provided for fatty acid biosynthesis in WJ11 compared with CBS 277.49.
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Comparative Proteome Analysis between High Lipid-Producing Strain Mucor circinelloides WJ11 and Low Lipid-Producing Strain CBS 277.49
2017Co-Authors: Xin Tang, Haiqin Chen, Hao Zhang, Yong Q. Chen, Wei ChenAbstract:Mucor circinelloides is one of few oleaginous fungi that produces a useful oil rich in γ-linolenic acid, but it usually only produces
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13 c metabolic flux analysis of lipid accumulation in the oleaginous fungus Mucor circinelloides
Bioresource Technology, 2015Co-Authors: Lina Zhao, Haiqin Chen, Wei Chen, Yong Q. Chen, H Zhang, Liping Wang, Yuanda SongAbstract:The oleaginous fungus Mucor circinelloides is of industrial interest because it can produce high levels of polyunsaturated fatty acid γ-linolenic acid. M. circinelloides CBS 277.49 is able to accumulate less than 15% of cell dry weight as lipids, while M. circinelloides WJ11 can accumulate lipid up to 36%. In order to better understand the mechanisms behind the differential lipid accumulation in these two strains, tracer experiments with (13)C-glucose were performed with the growth of M. circinelloides and subsequent gas chromatography-mass spectrometric detection of (13)C-patterns in proteinogenic amino acids was carried out to identify the metabolic network topology and estimate intracellular fluxes. Our results showed that the high oleaginous strain WJ11 had higher flux of pentose phosphate pathway and malic enzyme, lower flux in tricarboxylic acid cycle, higher flux in glyoxylate cycle and ATP: citrate lyase, together, it might provide more NADPH and substrate acetyl-CoA for fatty acid synthesis.
Xiangke Wang - One of the best experts on this subject based on the ideXlab platform.
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Bioaccumulation and transformation of U(VI) by sporangiospores of Mucor circinelloides
Chemical Engineering Journal, 2019Co-Authors: Wencheng Song, Xiangxue Wang, Yubing Sun, Tasawar Hayat, Xiangke WangAbstract:Abstract The bioaccumulation and transformation of U(VI) by sporangiospores of Mucor circinelloides under different environmental conditions (e.g., reaction time, pH, carbonate, sporangiospores concentration, and temperature) was investigated by batch, XPS and EXAFS techniques. The bioaccumulation kinetics and isotherms can be fitted by the pseudo-second-order kinetic mode and Langmuir model, respectively, due to the high correlation coefficient. The maximum bioaccumulation capacity of sporangiospores for U(VI) was 166.13 mg/g at pH 6.0, which was significantly higher than that of other mycelia or spores. The intracellular and extracellular morphology of sporangiospores were significantly changed after U(VI) bioaccumulation, and levels of intracellular H2O2, O3−, GPx and SOD compounds in sporangiospores increased significantly. XANES analysis confirmed that the intracellular U(VI) was reduced to U(IV) by sporangiospores, and U(IV) might be stably associated with oxygen-bearing functional groups by EXAFS analysis. These results show that the sporangiospores can be used a promising adsorbent for the bioaccumulation and transformation of U(VI) from aqueous solutions, which has important scientific significance for the immobilization of U(VI) in environmental remediation.
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enhanced immobilization of u vi on Mucor circinelloides in presence of as v batch and xafs investigation
Environmental Pollution, 2018Co-Authors: Wencheng Song, Xiangxue Wang, Tasawar Hayat, Xiangke Wang, Zhongshan Chen, Guodong ShengAbstract:The combined pollution of radionuclides and heavy metals has been given rise to widespread concern during uranium mining. The influence of As(V) on U(VI) immobilization by Mucor circinelloides (M. circinelloides) was investigated using batch experiments. The activity of antioxidative enzymes and concentrations of thiol compounds and organic acid in M. circinelloides increased to respond to different U(VI) and As(V) stress. The morphological structure of M. circinelloides changed obviously under U(VI) and As(V) stress by SEM and TEM analysis. The results of XANES and EXAFS analysis showed that U(VI) was mainly reduced to nano-uraninite (nano-UO2, 30.1%) in U400, while only 9.7% of nano-UO2 was observed in the presence of As(V) in U400-As400 due to the formation of uranyl arsenate precipitate (Trogerite, 48.6%). These observations will provide the fundamental data for fungal remediation of uranium and heavy metals in uranium-contaminated soils.