The Experts below are selected from a list of 66 Experts worldwide ranked by ideXlab platform
Masamitsu Futai - One of the best experts on this subject based on the ideXlab platform.
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Lipopolysaccharide-induced Multinuclear Cells: increased internalization of polystyrene beads and possible signals for Cell fusion.
Biochemical and biophysical research communications, 2013Co-Authors: Mayumi Nakanishi-matsui, Shio Yano, Masamitsu FutaiAbstract:A murine macrophage-derived line, RAW264.7, becomes Multinuclear on stimulation with lipopolysaccharide (LPS), an outer membrane component of Gram-negative bacteria. These Multinuclear Cells internalized more polystyrene beads than mononuclear Cells or osteoclasts (Nakanishi-Matsui, M., Yano, S., Matsumoto, N., and Futai, M., 2012). In this study, we analyzed the time courses of Cell fusion in the presence of large beads. They were internalized into Cells actively fusing to become Multinuclear. However, the Multinuclear Cells once formed showed only low phagocytosis activity. These results suggest that formation of the Multinuclear Cells and bead internalization took place simultaneously. The formation of Multinuclear Cells was blocked by inhibitors for phosphoinositide 3-kinase, phospholipase C, calcineurin, and c-Jun N-terminal kinase. In addition, interleukin 6 and 10 also exhibited inhibitory effects. These signaling molecules and cytokines may play a crucial role in the LPS-induced Multinuclear Cell formation.
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Lipopolysaccharide induces Multinuclear Cell from RAW264.7 line with increased phagocytosis activity.
Biochemical and biophysical research communications, 2012Co-Authors: Mayumi Nakanishi-matsui, Shio Yano, Naomi Matsumoto, Masamitsu FutaiAbstract:Abstract Lipopolysaccharide (LPS), an outer membrane component of Gram-negative bacteria, induces strong proinflammatory responses, including the release of cytokines and nitric oxide from macrophage. In this study, we found that a murine macrophage-derived line, RAW264.7, became Multinuclear through Cell-Cell fusion after incubation with highly purified LPS or synthetic lipid A in the presence of Ca2+. The same Cell line is known to differentiate into Multinuclear osteoclast, which expresses a specific proton pumping ATPase together with osteoclast markers on stimulation by the extraCellular domain of receptor activator of nuclear factor κB ligand (Toyomura, T., Murata, Y., Yamamoto, A., Oka, T., Sun-Wada, G.-H., Wada, Y. and Futai, M., 2003). The LPS-induced Multinuclear Cells did not express osteoclast-specific enzymes including tartrate-resistant acid phosphatase and cathepsin K. During Multinuclear Cell formation, the Cells internalized more and larger polystyrene beads (diameter 6–15 μm) than mononuclear Cells and osteoclasts. The internalized beads were located in lysosome-marker positive organelles, which were probably phagolysosomes. The LPS-induced Multinuclear Cell could be a good model system to study phagocytosis of large foreign bodies.
Hiromu Takematsu - One of the best experts on this subject based on the ideXlab platform.
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Sphingolipids involved in the induction of Multinuclear Cell formation.
Biochimica et biophysica acta, 2002Co-Authors: Yasunori Kozutsumi, Takayuki Kanazawa, Yidi Sun, Toshiyuki Yamaji, Harumi Yamamoto, Hiromu TakematsuAbstract:In this review, we focus on sphingolipids as potential regulators of the induction of Multinuclear Cell formation through the inhibition of cytokinesis. A sphingolipid, psychosine (Psy) (galactosylsphingosine), was demonstrated to be a trigger lipid for the inhibition of cytokinesis and the induction of Multinuclear giant Cells associated with a sphingolipid metabolic disease, globoid Cell leukodystrophy (GLD). Indeed, Psy is known to accumulate in the patients' brains. Interestingly, inhibition of sphingolipid biosynthesis also induced Multinuclear Cells. When Cells were treated with a new immunosuppressant, ISP-1/myriocin, which inhibits serine palmitoyltransferase, the first step enzyme of sphingolipid biosynthesis, the Cells underwent multinucleation and apoptosis. At present, a definitive model of the function of sphingolipids as to the induction of Multinuclear Cell formation is not available due to the rudimentary information but possible mechanisms are discussed.
Mayumi Nakanishi-matsui - One of the best experts on this subject based on the ideXlab platform.
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Lipopolysaccharide-induced Multinuclear Cells: increased internalization of polystyrene beads and possible signals for Cell fusion.
Biochemical and biophysical research communications, 2013Co-Authors: Mayumi Nakanishi-matsui, Shio Yano, Masamitsu FutaiAbstract:A murine macrophage-derived line, RAW264.7, becomes Multinuclear on stimulation with lipopolysaccharide (LPS), an outer membrane component of Gram-negative bacteria. These Multinuclear Cells internalized more polystyrene beads than mononuclear Cells or osteoclasts (Nakanishi-Matsui, M., Yano, S., Matsumoto, N., and Futai, M., 2012). In this study, we analyzed the time courses of Cell fusion in the presence of large beads. They were internalized into Cells actively fusing to become Multinuclear. However, the Multinuclear Cells once formed showed only low phagocytosis activity. These results suggest that formation of the Multinuclear Cells and bead internalization took place simultaneously. The formation of Multinuclear Cells was blocked by inhibitors for phosphoinositide 3-kinase, phospholipase C, calcineurin, and c-Jun N-terminal kinase. In addition, interleukin 6 and 10 also exhibited inhibitory effects. These signaling molecules and cytokines may play a crucial role in the LPS-induced Multinuclear Cell formation.
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Lipopolysaccharide induces Multinuclear Cell from RAW264.7 line with increased phagocytosis activity.
Biochemical and biophysical research communications, 2012Co-Authors: Mayumi Nakanishi-matsui, Shio Yano, Naomi Matsumoto, Masamitsu FutaiAbstract:Abstract Lipopolysaccharide (LPS), an outer membrane component of Gram-negative bacteria, induces strong proinflammatory responses, including the release of cytokines and nitric oxide from macrophage. In this study, we found that a murine macrophage-derived line, RAW264.7, became Multinuclear through Cell-Cell fusion after incubation with highly purified LPS or synthetic lipid A in the presence of Ca2+. The same Cell line is known to differentiate into Multinuclear osteoclast, which expresses a specific proton pumping ATPase together with osteoclast markers on stimulation by the extraCellular domain of receptor activator of nuclear factor κB ligand (Toyomura, T., Murata, Y., Yamamoto, A., Oka, T., Sun-Wada, G.-H., Wada, Y. and Futai, M., 2003). The LPS-induced Multinuclear Cells did not express osteoclast-specific enzymes including tartrate-resistant acid phosphatase and cathepsin K. During Multinuclear Cell formation, the Cells internalized more and larger polystyrene beads (diameter 6–15 μm) than mononuclear Cells and osteoclasts. The internalized beads were located in lysosome-marker positive organelles, which were probably phagolysosomes. The LPS-induced Multinuclear Cell could be a good model system to study phagocytosis of large foreign bodies.
Yasunori Kozutsumi - One of the best experts on this subject based on the ideXlab platform.
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Sphingolipids involved in the induction of Multinuclear Cell formation.
Biochimica et biophysica acta, 2002Co-Authors: Yasunori Kozutsumi, Takayuki Kanazawa, Yidi Sun, Toshiyuki Yamaji, Harumi Yamamoto, Hiromu TakematsuAbstract:In this review, we focus on sphingolipids as potential regulators of the induction of Multinuclear Cell formation through the inhibition of cytokinesis. A sphingolipid, psychosine (Psy) (galactosylsphingosine), was demonstrated to be a trigger lipid for the inhibition of cytokinesis and the induction of Multinuclear giant Cells associated with a sphingolipid metabolic disease, globoid Cell leukodystrophy (GLD). Indeed, Psy is known to accumulate in the patients' brains. Interestingly, inhibition of sphingolipid biosynthesis also induced Multinuclear Cells. When Cells were treated with a new immunosuppressant, ISP-1/myriocin, which inhibits serine palmitoyltransferase, the first step enzyme of sphingolipid biosynthesis, the Cells underwent multinucleation and apoptosis. At present, a definitive model of the function of sphingolipids as to the induction of Multinuclear Cell formation is not available due to the rudimentary information but possible mechanisms are discussed.
Shio Yano - One of the best experts on this subject based on the ideXlab platform.
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Lipopolysaccharide-induced Multinuclear Cells: increased internalization of polystyrene beads and possible signals for Cell fusion.
Biochemical and biophysical research communications, 2013Co-Authors: Mayumi Nakanishi-matsui, Shio Yano, Masamitsu FutaiAbstract:A murine macrophage-derived line, RAW264.7, becomes Multinuclear on stimulation with lipopolysaccharide (LPS), an outer membrane component of Gram-negative bacteria. These Multinuclear Cells internalized more polystyrene beads than mononuclear Cells or osteoclasts (Nakanishi-Matsui, M., Yano, S., Matsumoto, N., and Futai, M., 2012). In this study, we analyzed the time courses of Cell fusion in the presence of large beads. They were internalized into Cells actively fusing to become Multinuclear. However, the Multinuclear Cells once formed showed only low phagocytosis activity. These results suggest that formation of the Multinuclear Cells and bead internalization took place simultaneously. The formation of Multinuclear Cells was blocked by inhibitors for phosphoinositide 3-kinase, phospholipase C, calcineurin, and c-Jun N-terminal kinase. In addition, interleukin 6 and 10 also exhibited inhibitory effects. These signaling molecules and cytokines may play a crucial role in the LPS-induced Multinuclear Cell formation.
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Lipopolysaccharide induces Multinuclear Cell from RAW264.7 line with increased phagocytosis activity.
Biochemical and biophysical research communications, 2012Co-Authors: Mayumi Nakanishi-matsui, Shio Yano, Naomi Matsumoto, Masamitsu FutaiAbstract:Abstract Lipopolysaccharide (LPS), an outer membrane component of Gram-negative bacteria, induces strong proinflammatory responses, including the release of cytokines and nitric oxide from macrophage. In this study, we found that a murine macrophage-derived line, RAW264.7, became Multinuclear through Cell-Cell fusion after incubation with highly purified LPS or synthetic lipid A in the presence of Ca2+. The same Cell line is known to differentiate into Multinuclear osteoclast, which expresses a specific proton pumping ATPase together with osteoclast markers on stimulation by the extraCellular domain of receptor activator of nuclear factor κB ligand (Toyomura, T., Murata, Y., Yamamoto, A., Oka, T., Sun-Wada, G.-H., Wada, Y. and Futai, M., 2003). The LPS-induced Multinuclear Cells did not express osteoclast-specific enzymes including tartrate-resistant acid phosphatase and cathepsin K. During Multinuclear Cell formation, the Cells internalized more and larger polystyrene beads (diameter 6–15 μm) than mononuclear Cells and osteoclasts. The internalized beads were located in lysosome-marker positive organelles, which were probably phagolysosomes. The LPS-induced Multinuclear Cell could be a good model system to study phagocytosis of large foreign bodies.