The Experts below are selected from a list of 318 Experts worldwide ranked by ideXlab platform
F Ferre - One of the best experts on this subject based on the ideXlab platform.
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regulation of protein kinase c in the Muscular Layer of human placental stem villi vessels
FEBS Letters, 1998Co-Authors: C Tertrinclary, Thierry Fournier, F FerreAbstract:Protein kinase C (PKC) activity in the Muscular Layer of stem villi vessels from the human term placenta was studied. Resting state PKC activity was distributed evenly between the cytosol and the particulate fractions. Upon stimulation by three different activators, phorbol 12-myristate 13-acetate, fluoride and endothelin-1, a translocation of PKC activity from the cytosolic to the particulate fraction was observed. The expression and distribution of PKC isoforms were then examined by Western blot analysis using specific antibodies to PKC isoforms. At least four PKC isoforms, PKCα, PKCβ1, PKCβ2, PKCζ, and trace amounts of PKCϵ were detected in both fractions. Their relative responses to the different agonists were examined by quantifying their subcellular redistribution. No significant differential activation of the four mainly expressed PKC isoforms were observed in response to stimulation with any of the stimuli. Moreover, our results show that endothelin-1 induced translocation/activation of PKC in this vascular smooth muscle.
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identification and characterization of 125i insulin like growth factor ii binding sites on the Muscular Layer of stem villi vessels of human term placenta
Biology of Reproduction, 1996Co-Authors: R Rebourcet, S Deborde, F De Ceuninck, J Willeput, F FerreAbstract:The primary function of the placenta is to ensure an optimal environment for fetal growth and development. In normal pregnancy, placental vascular tone regulation assures fetus well-being and normal development by maintaining adequate blood flow so as to ensure materno-fetal exchanges. In human placenta, synthesis of insulin-like growth factor (IGF)-II and specific binding sites have been previously characterized in the trophoblast; in contrast, no studies have dealt with this subject in the fetoplacental vascular system, particularly in stem villi vessels. We thus investigated whether membranes of the Muscular Layer of stem villi vessels contained 125 I-IGF-II binding sites. Two complementary approaches were used : 125 I-IGF-II binding and affinity cross-linking studies. 125 I-IGF-II labeled, in a saturable and noncooperative manner, a single class of high-affinity binding sites characterized by a K d of 1.24 ± 0.26 nM (n = 6), a maximum binding capacity (B max ) of 3.02 ± 0.45 pmol/mg protein, and a Hill coefficient of 1.00 ± 0.15. Competitors for 125 I-IGF-II binding to membranes were in the order of potency IGF-II > IGF-I. Insulin was not a competitor. Affinity cross-linking of membranes with 125 I-IGF-II, followed by SDS-PAGE and autoradiography, revealed two labeled bands : a protein complex of 250 kDa, which corresponds to the type II IGF receptor, and another of 135 kDa, corresponding to the type I IGF receptor. Only IGF-II could displace 125 I-IGF-II binding from the major 250-kDa band, while 125 I-IGF-II bound to the minor 135-kDa band was displaced by either IGF-I, IGF-II, or insulin. In conclusion, high levels of specific binding sites for 125 I-IGF-II are present in the Muscular Layer of stem villi vessels, which are considered placenta resistance vessels. The involvement of both type I and type II IGF receptors in the growth-promoting action of IGF-II remains to be determined in the fetoplacental vascular system.
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endothelin induced phosphoinositide hydrolysis in the Muscular Layer of stem villi vessels of human term placenta
European Journal of Endocrinology, 1995Co-Authors: Francoise Mondon, Florence Douallabell Kotto Maka, Sana Sabry, F FerreAbstract:In the present study, we examined the relationship between endothelin receptors and phosphoinositide breakdown in muscle explants of placental stem villi vessels. All peptides examined, i.e. endothelin-1 (ET-1), ET-3, sarafotoxin 6b (S6b) and S6c, were able to induce phosphoinositide hydrolysis in a dose-dependent manner ; ET-1 was more potent than S6b and ET-3, with corresponding EC 50 values of 44 ± 16 pmol/l, 18 ± 13 nmol/l and 33 ± 24 nmol/l, respectively. Sarafotoxin induced only moderate stimulation of inositol phosphate accumulation. Both ET-1- and S6b-induced accumulation of inositol phosphate was almost totally (90%) inhibited by 100 μmol/l BQ 123, while the S6c response was not affected by the ET A receptor antagonist. In contrast, the ET B receptor antagonist IRL 1038 inhibited S6c-induced inositol phosphate accumulation by more than 80%, whereas inhibition was only about 30% for ET-1 and S6b stimulations. This indicates that both ET A and ET B receptors were coupled to the phospholipase C transducing system in the Muscular Layer of placental stem villi vessels, and there is evidence that the phosphoinositide hydrolysis response is obtained predominantly via ET A receptor activation.
John R Kimoff - One of the best experts on this subject based on the ideXlab platform.
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upper airway muscle inflammation and denervation changes in obstructive sleep apnea
American Journal of Respiratory and Critical Care Medicine, 2004Co-Authors: John H Boyd, Basil J. Petrof, Qutayba Hamid, Richard Fraser, John R KimoffAbstract:Inflammatory cell infiltration and afferent neuropathy have been shown in the upper airway (UA) mucosa of subjects with obstructive sleep apnea (OSA). We hypothesized that inflammatory and denervation changes also involve the Muscular Layer of the pharynx in OSA. Morphometric analysis was performed on UA tissue from nonsnoring control subjects (n = 7) and patients with OSA (n = 11) following palatal surgery. As compared with control subjects, inflammatory cells were increased in the Muscular Layer of patients with OSA, with CD4+ and activated CD25+ T cells (both increased approximately threefold) predominating. Inflammation was also present in UA mucosa, but with a different pattern consisting of CD8+ (2.8-fold increase) and activated CD25+ (3.2-fold increase) T cell predominance. As ascertained by immunoreactivity for the panneuronal marker PGP9.5, there was a dramatic (5.7-fold) increase in intraMuscular nerve fibers in OSA patients compared with control subjects, as well as direct evidence of denervation based on positive immunostaining of the muscle fiber sarcolemmal membrane for the neural cell adhesion molecule in patients with OSA. These data suggest that inflammatory cell infiltration and denervation changes affect not only the mucosa, but also the UA muscle of patients with OSA. This may have important implications for the ability to generate adequate Muscular dilating forces during sleep.
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upper airway muscle inflammation and denervation changes in obstructive sleep apnea
American Journal of Respiratory and Critical Care Medicine, 2004Co-Authors: John H Boyd, Basil J. Petrof, Qutayba Hamid, Richard Fraser, John R KimoffAbstract:Inflammatory cell infiltration and afferent neuropathy have been shown in the upper airway (UA) mucosa of subjects with obstructive sleep apnea (OSA). We hypothesized that inflammatory and denervation changes also involve the Muscular Layer of the pharynx in OSA. Morphometric analysis was performed on UA tissue from nonsnoring control subjects (n = 7) and patients with OSA (n = 11) following palatal surgery. As compared with control subjects, inflammatory cells were increased in the Muscular Layer of patients with OSA, with CD4+ and activated CD25+ T cells (both increased ∼ threefold) predominating. Inflammation was also present in UA mucosa, but with a different pattern consisting of CD8+ (2.8-fold increase) and activated CD25+ (3.2-fold increase) T cell predominance. As ascertained by immunoreactivity for the panneuronal marker PGP9.5, there was a dramatic (5.7-fold) increase in intraMuscular nerve fibers in OSA patients compared with control subjects, as well as direct evidence of denervation based on ...
John H Boyd - One of the best experts on this subject based on the ideXlab platform.
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upper airway muscle inflammation and denervation changes in obstructive sleep apnea
American Journal of Respiratory and Critical Care Medicine, 2004Co-Authors: John H Boyd, Basil J. Petrof, Qutayba Hamid, Richard Fraser, John R KimoffAbstract:Inflammatory cell infiltration and afferent neuropathy have been shown in the upper airway (UA) mucosa of subjects with obstructive sleep apnea (OSA). We hypothesized that inflammatory and denervation changes also involve the Muscular Layer of the pharynx in OSA. Morphometric analysis was performed on UA tissue from nonsnoring control subjects (n = 7) and patients with OSA (n = 11) following palatal surgery. As compared with control subjects, inflammatory cells were increased in the Muscular Layer of patients with OSA, with CD4+ and activated CD25+ T cells (both increased approximately threefold) predominating. Inflammation was also present in UA mucosa, but with a different pattern consisting of CD8+ (2.8-fold increase) and activated CD25+ (3.2-fold increase) T cell predominance. As ascertained by immunoreactivity for the panneuronal marker PGP9.5, there was a dramatic (5.7-fold) increase in intraMuscular nerve fibers in OSA patients compared with control subjects, as well as direct evidence of denervation based on positive immunostaining of the muscle fiber sarcolemmal membrane for the neural cell adhesion molecule in patients with OSA. These data suggest that inflammatory cell infiltration and denervation changes affect not only the mucosa, but also the UA muscle of patients with OSA. This may have important implications for the ability to generate adequate Muscular dilating forces during sleep.
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upper airway muscle inflammation and denervation changes in obstructive sleep apnea
American Journal of Respiratory and Critical Care Medicine, 2004Co-Authors: John H Boyd, Basil J. Petrof, Qutayba Hamid, Richard Fraser, John R KimoffAbstract:Inflammatory cell infiltration and afferent neuropathy have been shown in the upper airway (UA) mucosa of subjects with obstructive sleep apnea (OSA). We hypothesized that inflammatory and denervation changes also involve the Muscular Layer of the pharynx in OSA. Morphometric analysis was performed on UA tissue from nonsnoring control subjects (n = 7) and patients with OSA (n = 11) following palatal surgery. As compared with control subjects, inflammatory cells were increased in the Muscular Layer of patients with OSA, with CD4+ and activated CD25+ T cells (both increased ∼ threefold) predominating. Inflammation was also present in UA mucosa, but with a different pattern consisting of CD8+ (2.8-fold increase) and activated CD25+ (3.2-fold increase) T cell predominance. As ascertained by immunoreactivity for the panneuronal marker PGP9.5, there was a dramatic (5.7-fold) increase in intraMuscular nerve fibers in OSA patients compared with control subjects, as well as direct evidence of denervation based on ...
Basil J. Petrof - One of the best experts on this subject based on the ideXlab platform.
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upper airway muscle inflammation and denervation changes in obstructive sleep apnea
American Journal of Respiratory and Critical Care Medicine, 2004Co-Authors: John H Boyd, Basil J. Petrof, Qutayba Hamid, Richard Fraser, John R KimoffAbstract:Inflammatory cell infiltration and afferent neuropathy have been shown in the upper airway (UA) mucosa of subjects with obstructive sleep apnea (OSA). We hypothesized that inflammatory and denervation changes also involve the Muscular Layer of the pharynx in OSA. Morphometric analysis was performed on UA tissue from nonsnoring control subjects (n = 7) and patients with OSA (n = 11) following palatal surgery. As compared with control subjects, inflammatory cells were increased in the Muscular Layer of patients with OSA, with CD4+ and activated CD25+ T cells (both increased approximately threefold) predominating. Inflammation was also present in UA mucosa, but with a different pattern consisting of CD8+ (2.8-fold increase) and activated CD25+ (3.2-fold increase) T cell predominance. As ascertained by immunoreactivity for the panneuronal marker PGP9.5, there was a dramatic (5.7-fold) increase in intraMuscular nerve fibers in OSA patients compared with control subjects, as well as direct evidence of denervation based on positive immunostaining of the muscle fiber sarcolemmal membrane for the neural cell adhesion molecule in patients with OSA. These data suggest that inflammatory cell infiltration and denervation changes affect not only the mucosa, but also the UA muscle of patients with OSA. This may have important implications for the ability to generate adequate Muscular dilating forces during sleep.
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upper airway muscle inflammation and denervation changes in obstructive sleep apnea
American Journal of Respiratory and Critical Care Medicine, 2004Co-Authors: John H Boyd, Basil J. Petrof, Qutayba Hamid, Richard Fraser, John R KimoffAbstract:Inflammatory cell infiltration and afferent neuropathy have been shown in the upper airway (UA) mucosa of subjects with obstructive sleep apnea (OSA). We hypothesized that inflammatory and denervation changes also involve the Muscular Layer of the pharynx in OSA. Morphometric analysis was performed on UA tissue from nonsnoring control subjects (n = 7) and patients with OSA (n = 11) following palatal surgery. As compared with control subjects, inflammatory cells were increased in the Muscular Layer of patients with OSA, with CD4+ and activated CD25+ T cells (both increased ∼ threefold) predominating. Inflammation was also present in UA mucosa, but with a different pattern consisting of CD8+ (2.8-fold increase) and activated CD25+ (3.2-fold increase) T cell predominance. As ascertained by immunoreactivity for the panneuronal marker PGP9.5, there was a dramatic (5.7-fold) increase in intraMuscular nerve fibers in OSA patients compared with control subjects, as well as direct evidence of denervation based on ...
Richard Fraser - One of the best experts on this subject based on the ideXlab platform.
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upper airway muscle inflammation and denervation changes in obstructive sleep apnea
American Journal of Respiratory and Critical Care Medicine, 2004Co-Authors: John H Boyd, Basil J. Petrof, Qutayba Hamid, Richard Fraser, John R KimoffAbstract:Inflammatory cell infiltration and afferent neuropathy have been shown in the upper airway (UA) mucosa of subjects with obstructive sleep apnea (OSA). We hypothesized that inflammatory and denervation changes also involve the Muscular Layer of the pharynx in OSA. Morphometric analysis was performed on UA tissue from nonsnoring control subjects (n = 7) and patients with OSA (n = 11) following palatal surgery. As compared with control subjects, inflammatory cells were increased in the Muscular Layer of patients with OSA, with CD4+ and activated CD25+ T cells (both increased approximately threefold) predominating. Inflammation was also present in UA mucosa, but with a different pattern consisting of CD8+ (2.8-fold increase) and activated CD25+ (3.2-fold increase) T cell predominance. As ascertained by immunoreactivity for the panneuronal marker PGP9.5, there was a dramatic (5.7-fold) increase in intraMuscular nerve fibers in OSA patients compared with control subjects, as well as direct evidence of denervation based on positive immunostaining of the muscle fiber sarcolemmal membrane for the neural cell adhesion molecule in patients with OSA. These data suggest that inflammatory cell infiltration and denervation changes affect not only the mucosa, but also the UA muscle of patients with OSA. This may have important implications for the ability to generate adequate Muscular dilating forces during sleep.
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upper airway muscle inflammation and denervation changes in obstructive sleep apnea
American Journal of Respiratory and Critical Care Medicine, 2004Co-Authors: John H Boyd, Basil J. Petrof, Qutayba Hamid, Richard Fraser, John R KimoffAbstract:Inflammatory cell infiltration and afferent neuropathy have been shown in the upper airway (UA) mucosa of subjects with obstructive sleep apnea (OSA). We hypothesized that inflammatory and denervation changes also involve the Muscular Layer of the pharynx in OSA. Morphometric analysis was performed on UA tissue from nonsnoring control subjects (n = 7) and patients with OSA (n = 11) following palatal surgery. As compared with control subjects, inflammatory cells were increased in the Muscular Layer of patients with OSA, with CD4+ and activated CD25+ T cells (both increased ∼ threefold) predominating. Inflammation was also present in UA mucosa, but with a different pattern consisting of CD8+ (2.8-fold increase) and activated CD25+ (3.2-fold increase) T cell predominance. As ascertained by immunoreactivity for the panneuronal marker PGP9.5, there was a dramatic (5.7-fold) increase in intraMuscular nerve fibers in OSA patients compared with control subjects, as well as direct evidence of denervation based on ...
