The Experts below are selected from a list of 10146 Experts worldwide ranked by ideXlab platform
Monty W Reichert - One of the best experts on this subject based on the ideXlab platform.
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Mylar and teflon af as cell culture substrates for studying endothelial cell adhesion
Biomaterials, 2005Co-Authors: Charles C. Anamelechi, George A. Truskey, Monty W ReichertAbstract:Abstract The textured and opaque nature of Dacron™ and ePTFE has prevented the use of these fabrics in conventional cell culture techniques normally employed to optimize cell attachment and retention. This lack of optimization has led, in part, to the poor performance of endothelialization strategies for improving vascular graft patency. Here we show that thin, transparent films of Mylar™ and Teflon-AF™ are viable in vitro cell culture mimics of Dacron™ and ePTFE vascular graft materials, particularly for the study of protein mediated endothelial cell (EC) attachment, spreading and adhesion. Glass substrates were used as controls. X-ray photoelectron spectroscopy (XPS) and contact angle analysis showed that Mylar™ and Teflon-AF™ have surface chemistries that closely match Dacron™ and ePTFE. 125 I radiolabeling was used to quantify fibronectin (FN) adsorption, and FN and biotinylated-BSA “dual ligand” co-adsorption onto glass, Mylar™ and Teflon-AF™ substrates. Native human umbilical vein endothelial cells (HUVEC) and streptavidin-incubated biotinylated-HUVEC (SA-b-HUVEC) spreading was measured using phase contrast microscopy. Cell retention and adhesion was determined using phase contrast microscopy under laminar flow. All surfaces lacking protein pre-treatment, regardless of surface type, showed the lowest degree of cell spreading and retention. Dual ligand treated Mylar™ films showed significantly greater SA-b-HUVEC spreading up to 2 h, but were similar to HUVEC on FN treated Mylar™ at longer times; whereas SA-b-HUVEC spreading on dual ligand treated Teflon-AF was never significantly different from HUVEC on FN treated Teflon-AF™ at any time point. SA-b-HUVEC retention was significantly greater on dual ligand treated Mylar™ compared to HUVEC on FN treated Mylar™ over the entire range of shear stresses tested (3.54–28.3 dynes/cm 2 ); whereas SA-b-HUVEC retention to dual ligand and HUVEC retention to FN treated Teflon-AF™ gave similar results at each shear stress, with only the mid-range of stresses showing significant difference in cell retention to Teflon-AF™.
Matthew R Linford - One of the best experts on this subject based on the ideXlab platform.
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carbon ternary alloy carbon optical stack on Mylar as an optical data storage medium to potentially replace magnetic tape
ACS Applied Materials & Interfaces, 2013Co-Authors: Hao Wang, Barry M Lunt, Richard J Gates, Matthew C Asplund, V Shutthanandan, Robert C Davis, Matthew R LinfordAbstract:A novel write-once-read-many (WORM) optical stack on Mylar tape is proposed as a replacement for magnetic tape for archival data storage. This optical tape contains a cosputtered bismuth–tellurium–selenium (BTS) alloy as the write layer sandwiched between thin, protective films of reactively sputtered carbon. The composition and thickness of the BTS layer were confirmed by Rutherford Backscattering (RBS) and atomic force microscopy (AFM), respectively. The C/BTS/C stack on Mylar was written to/marked by 532 nm laser pulses. Under the same conditions, control Mylar films without the optical stack were unaffected. Marks, which showed craters/movement of the write material, were characterized by optical microscopy and AFM. The threshold laser powers for making marks on C/BTS/C stacks with different thicknesses were explored. Higher quality marks were made with a 60× objective compared to a 40× objective in our marking apparatus. The laser writing process was simulated with COMSOL.
Charles C. Anamelechi - One of the best experts on this subject based on the ideXlab platform.
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Mylar™ and Teflon-AF™ as cell culture substrates for studying endothelial cell adhesion
Biomaterials, 2005Co-Authors: Charles C. Anamelechi, George A. Truskey, W. Monty ReichertAbstract:Abstract The textured and opaque nature of Dacron™ and ePTFE has prevented the use of these fabrics in conventional cell culture techniques normally employed to optimize cell attachment and retention. This lack of optimization has led, in part, to the poor performance of endothelialization strategies for improving vascular graft patency. Here we show that thin, transparent films of Mylar™ and Teflon-AF™ are viable in vitro cell culture mimics of Dacron™ and ePTFE vascular graft materials, particularly for the study of protein mediated endothelial cell (EC) attachment, spreading and adhesion. Glass substrates were used as controls. X-ray photoelectron spectroscopy (XPS) and contact angle analysis showed that Mylar™ and Teflon-AF™ have surface chemistries that closely match Dacron™ and ePTFE. 125 I radiolabeling was used to quantify fibronectin (FN) adsorption, and FN and biotinylated-BSA “dual ligand” co-adsorption onto glass, Mylar™ and Teflon-AF™ substrates. Native human umbilical vein endothelial cells (HUVEC) and streptavidin-incubated biotinylated-HUVEC (SA-b-HUVEC) spreading was measured using phase contrast microscopy. Cell retention and adhesion was determined using phase contrast microscopy under laminar flow. All surfaces lacking protein pre-treatment, regardless of surface type, showed the lowest degree of cell spreading and retention. Dual ligand treated Mylar™ films showed significantly greater SA-b-HUVEC spreading up to 2 h, but were similar to HUVEC on FN treated Mylar™ at longer times; whereas SA-b-HUVEC spreading on dual ligand treated Teflon-AF was never significantly different from HUVEC on FN treated Teflon-AF™ at any time point. SA-b-HUVEC retention was significantly greater on dual ligand treated Mylar™ compared to HUVEC on FN treated Mylar™ over the entire range of shear stresses tested (3.54–28.3 dynes/cm 2 ); whereas SA-b-HUVEC retention to dual ligand and HUVEC retention to FN treated Teflon-AF™ gave similar results at each shear stress, with only the mid-range of stresses showing significant difference in cell retention to Teflon-AF™.
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Mylar and teflon af as cell culture substrates for studying endothelial cell adhesion
Biomaterials, 2005Co-Authors: Charles C. Anamelechi, George A. Truskey, Monty W ReichertAbstract:Abstract The textured and opaque nature of Dacron™ and ePTFE has prevented the use of these fabrics in conventional cell culture techniques normally employed to optimize cell attachment and retention. This lack of optimization has led, in part, to the poor performance of endothelialization strategies for improving vascular graft patency. Here we show that thin, transparent films of Mylar™ and Teflon-AF™ are viable in vitro cell culture mimics of Dacron™ and ePTFE vascular graft materials, particularly for the study of protein mediated endothelial cell (EC) attachment, spreading and adhesion. Glass substrates were used as controls. X-ray photoelectron spectroscopy (XPS) and contact angle analysis showed that Mylar™ and Teflon-AF™ have surface chemistries that closely match Dacron™ and ePTFE. 125 I radiolabeling was used to quantify fibronectin (FN) adsorption, and FN and biotinylated-BSA “dual ligand” co-adsorption onto glass, Mylar™ and Teflon-AF™ substrates. Native human umbilical vein endothelial cells (HUVEC) and streptavidin-incubated biotinylated-HUVEC (SA-b-HUVEC) spreading was measured using phase contrast microscopy. Cell retention and adhesion was determined using phase contrast microscopy under laminar flow. All surfaces lacking protein pre-treatment, regardless of surface type, showed the lowest degree of cell spreading and retention. Dual ligand treated Mylar™ films showed significantly greater SA-b-HUVEC spreading up to 2 h, but were similar to HUVEC on FN treated Mylar™ at longer times; whereas SA-b-HUVEC spreading on dual ligand treated Teflon-AF was never significantly different from HUVEC on FN treated Teflon-AF™ at any time point. SA-b-HUVEC retention was significantly greater on dual ligand treated Mylar™ compared to HUVEC on FN treated Mylar™ over the entire range of shear stresses tested (3.54–28.3 dynes/cm 2 ); whereas SA-b-HUVEC retention to dual ligand and HUVEC retention to FN treated Teflon-AF™ gave similar results at each shear stress, with only the mid-range of stresses showing significant difference in cell retention to Teflon-AF™.
Anna M. Lackner - One of the best experts on this subject based on the ideXlab platform.
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Mylar‐film‐compensated π and parallel‐aligned liquid crystal cells for direct‐view and projection displays
Applied Physics Letters, 1994Co-Authors: Anna M. LacknerAbstract:Optical properties of stretched Mylar films [poly(ethylene terephthalate)] were evaluated. Similar birefringence dispersion to liquid crystals over a wide spectral range makes Mylar an attractive phase retardation plate for display applications. Two display modes employing an antiparallel (or π) and a parallel‐aligned cell with a Mylar phase compensator were demonstrated. The film‐compensated π cell shows an 80:1 black‐to‐white contrast ratio, 60 Hz frame rate and ±45° viewing angle, and is attractive for direct view display. The film‐compensated parallel cell shows an 85:1 black‐to‐white contrast ratio, 180 Hz frame rate, and ±10° viewing angle, and is suitable for projection display.
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Mylar film compensated π and parallel aligned liquid crystal cells for direct view and projection displays
Applied Physics Letters, 1994Co-Authors: Anna M. LacknerAbstract:Optical properties of stretched Mylar films [poly(ethylene terephthalate)] were evaluated. Similar birefringence dispersion to liquid crystals over a wide spectral range makes Mylar an attractive phase retardation plate for display applications. Two display modes employing an antiparallel (or π) and a parallel‐aligned cell with a Mylar phase compensator were demonstrated. The film‐compensated π cell shows an 80:1 black‐to‐white contrast ratio, 60 Hz frame rate and ±45° viewing angle, and is attractive for direct view display. The film‐compensated parallel cell shows an 85:1 black‐to‐white contrast ratio, 180 Hz frame rate, and ±10° viewing angle, and is suitable for projection display.
Hao Wang - One of the best experts on this subject based on the ideXlab platform.
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carbon ternary alloy carbon optical stack on Mylar as an optical data storage medium to potentially replace magnetic tape
ACS Applied Materials & Interfaces, 2013Co-Authors: Hao Wang, Barry M Lunt, Richard J Gates, Matthew C Asplund, V Shutthanandan, Robert C Davis, Matthew R LinfordAbstract:A novel write-once-read-many (WORM) optical stack on Mylar tape is proposed as a replacement for magnetic tape for archival data storage. This optical tape contains a cosputtered bismuth–tellurium–selenium (BTS) alloy as the write layer sandwiched between thin, protective films of reactively sputtered carbon. The composition and thickness of the BTS layer were confirmed by Rutherford Backscattering (RBS) and atomic force microscopy (AFM), respectively. The C/BTS/C stack on Mylar was written to/marked by 532 nm laser pulses. Under the same conditions, control Mylar films without the optical stack were unaffected. Marks, which showed craters/movement of the write material, were characterized by optical microscopy and AFM. The threshold laser powers for making marks on C/BTS/C stacks with different thicknesses were explored. Higher quality marks were made with a 60× objective compared to a 40× objective in our marking apparatus. The laser writing process was simulated with COMSOL.
