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Jonathan P A Gardner - One of the best experts on this subject based on the ideXlab platform.
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genetic diversity of southern hemisphere blue mussels bivalvia mytilidae and the identification of non indigenous taxa
Biological Journal of The Linnean Society, 2010Co-Authors: Kristen M Westfall, Jonathan P A GardnerAbstract:The taxonomic and evolutionary affinities of Southern hemisphere smooth-shelled blue mussels are unclear, with studies using different marker types having identified different relationships among various geographic regions. Using an existing and a new molecular assay, the present study builds on previous work to test the distribution of blue mussels native to and introduced to the Southern hemisphere. Populations of Mytilus were sampled from New Zealand, Australia, and Chile. The nuclear-DNA marker Me 15/16 was used to identify the taxonomic status of 484 individuals. A new restriction fragment length polymorphism (RFLP) assay was used to identify the hemisphere of origin for a subset of Mytilus galloprovincialis. The Me15/16 marker identified 478 pure M. galloprovincialis from Southern hemisphere sites and six Mytilus edulis/M. galloprovincialis hybrids from the Auckland Islands (New Zealand) and Chile. A cytoplasmic RFLP identified Northern hemisphere M. galloprovincialis in almost every Southern hemisphere region. The presence of native M. galloprovincialis at high latitudes (up to 52°S) has implications for our understanding of environmentally induced selective constraints considered to determine species distributions. Widespread occurrence of invasive Northern hemisphere blue mussels in the Southern hemisphere is documented for the first time. Identification of inter-specific hybrids (M. edulis ¥ M. galloprovincialis) in Chile and in the Auckland Islands (subantarctic New Zealand) illustrates that environments ranging from international ports to remote protected locations are vulnerable to bioinvasion. © 2010 The Linnean Society of London, Biological Journal of the Linnean Society, 2010, 101, 898‐909.
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an rflp assay to determine if Mytilus galloprovincialis lmk mytilidae bivalvia is of northern or southern hemisphere origin
Molecular Ecology Resources, 2010Co-Authors: Kristen M Westfall, Peter H Wimberger, Jonathan P A GardnerAbstract:Mytilus galloprovincialis is one of three smooth shelled blue mussel species belonging to the Mytilus edulis species complex. Naturally occurring and introduced populations of M. galloprovincialis are widely distributed throughout many regions of the globe. Mytilus galloprovincialis includes morphologically indistinguishable Northern and Southern hemisphere mtDNA lineages that have been separated for ∼1 my. To distinguish recently introduced Northern M. galloprovincialis from resident Southern M. galloprovincialis in New Zealand, we developed a 16s rRNA RFLP assay. We compared RFLP assignments of 178 mussels with those generated from a 16s rRNA sequence-estimated phylogeny. All mussels were correctly assigned by the RFLP to their sequence-based phylogenetic placement. This assay allows the rapid identification of Northern and Southern hemisphere M. galloprovincialis and will provide an important tool for monitoring human mediated introductions of otherwise cryptic lineages.
Kristen M Westfall - One of the best experts on this subject based on the ideXlab platform.
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genetic diversity of southern hemisphere blue mussels bivalvia mytilidae and the identification of non indigenous taxa
Biological Journal of The Linnean Society, 2010Co-Authors: Kristen M Westfall, Jonathan P A GardnerAbstract:The taxonomic and evolutionary affinities of Southern hemisphere smooth-shelled blue mussels are unclear, with studies using different marker types having identified different relationships among various geographic regions. Using an existing and a new molecular assay, the present study builds on previous work to test the distribution of blue mussels native to and introduced to the Southern hemisphere. Populations of Mytilus were sampled from New Zealand, Australia, and Chile. The nuclear-DNA marker Me 15/16 was used to identify the taxonomic status of 484 individuals. A new restriction fragment length polymorphism (RFLP) assay was used to identify the hemisphere of origin for a subset of Mytilus galloprovincialis. The Me15/16 marker identified 478 pure M. galloprovincialis from Southern hemisphere sites and six Mytilus edulis/M. galloprovincialis hybrids from the Auckland Islands (New Zealand) and Chile. A cytoplasmic RFLP identified Northern hemisphere M. galloprovincialis in almost every Southern hemisphere region. The presence of native M. galloprovincialis at high latitudes (up to 52°S) has implications for our understanding of environmentally induced selective constraints considered to determine species distributions. Widespread occurrence of invasive Northern hemisphere blue mussels in the Southern hemisphere is documented for the first time. Identification of inter-specific hybrids (M. edulis ¥ M. galloprovincialis) in Chile and in the Auckland Islands (subantarctic New Zealand) illustrates that environments ranging from international ports to remote protected locations are vulnerable to bioinvasion. © 2010 The Linnean Society of London, Biological Journal of the Linnean Society, 2010, 101, 898‐909.
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an rflp assay to determine if Mytilus galloprovincialis lmk mytilidae bivalvia is of northern or southern hemisphere origin
Molecular Ecology Resources, 2010Co-Authors: Kristen M Westfall, Peter H Wimberger, Jonathan P A GardnerAbstract:Mytilus galloprovincialis is one of three smooth shelled blue mussel species belonging to the Mytilus edulis species complex. Naturally occurring and introduced populations of M. galloprovincialis are widely distributed throughout many regions of the globe. Mytilus galloprovincialis includes morphologically indistinguishable Northern and Southern hemisphere mtDNA lineages that have been separated for ∼1 my. To distinguish recently introduced Northern M. galloprovincialis from resident Southern M. galloprovincialis in New Zealand, we developed a 16s rRNA RFLP assay. We compared RFLP assignments of 178 mussels with those generated from a 16s rRNA sequence-estimated phylogeny. All mussels were correctly assigned by the RFLP to their sequence-based phylogenetic placement. This assay allows the rapid identification of Northern and Southern hemisphere M. galloprovincialis and will provide an important tool for monitoring human mediated introductions of otherwise cryptic lineages.
Matthew James Harrington - One of the best experts on this subject based on the ideXlab platform.
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A new twist on sea silk: the peculiar protein ultrastructure of fan shell and pearl oyster byssus.
Soft Matter, 2018Co-Authors: Delphine Pasche, Nils Horbelt, Frederic Marin, Sebastien Motreuil, Peter Fratzl, Giuseppe Falini, Elena Macías-sánchez, Dong Soo Hwang, Matthew James HarringtonAbstract:Numerous mussel species produce byssal threads - tough proteinaceous fibers, which anchor mussels in aquatic habitats. Byssal threads from Mytilus species, which are comprised of modified collagen proteins - have become a veritable archetype for bio-inspired polymers due to their self-healing properties. However, threads from different species are comparatively much less understood. In particular, the byssus of Pinna nobilis comprises thousands of fine fibers utilized by humans for millennia to fashion lightweight golden fabrics known as sea silk. P. nobilis is very different from Mytilus from an ecological, morphological and evolutionary point of view and it stands to reason that the structure-function relationships of its byssus are distinct. Here, we performed compositional analysis, X-ray diffraction (XRD) and transmission electron microscopy (TEM) to investigate byssal threads of P. nobilis, as well as a closely related bivalve species (Atrina pectinata) and a distantly related one (Pinctada fucata). This comparative investigation revealed that all three threads share a similar molecular superstructure comprised of globular proteins organized helically into nanofibrils, which is completely distinct from the Mytilus thread ultrastructure, and more akin to the supramolecular organization of bacterial pili and F-actin. This unexpected discovery hints at a possible divergence in byssus evolution in Pinnidae mussels, perhaps related to selective pressures in their respective ecological niches.
Diogène Jorge - One of the best experts on this subject based on the ideXlab platform.
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A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish
'Elsevier BV', 2021Co-Authors: Campàs Mònica, Reverté Jaume, Rambla-alegre Maria, Campbell Katrina, Gerssen Arjen, Diogène JorgeAbstract:Tetrodotoxin (TTX) is a potent neurotoxin responsible for many food poisoning incidents and some fatalities. Although mainly associated with the consumption of pufferfish, in recent years, TTX has been found in shellfish, particularly in Europe. In this work, a magnetic bead (MB)-based colorimetric immunoassay was applied to the detection of TTX in Pacific oysters (Crassostrea gigas), razor clams (Solen marginatus) and mussels (Mytilus galloprovincialis). Effective LODs (eLODs) for TTX of 1 μg/kg in oysters and razor clams and 3.3 μg/kg in mussels, significantly below the EFSA guidance threshold (44 μg/kg), were obtained. The strategy was applied to the analysis of naturally-contaminated Pacific oysters (Crassostrea gigas) and mussels (Mytilus edulis) from the Netherlands, and TTX was detected in all samples. The approach, which takes less than 1.5 h, proved to be useful as a rapid and simple method to detect TTX, support shellfish safety and protect consumers.info:eu-repo/semantics/acceptedVersio
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A fast magnetic bead-based colorimetric immunoassay for the detection of tetrodotoxins in shellfish
2020Co-Authors: Campàs Mònica, Reverté Jaume, Rambla-alegre Maria, Campbell Katrina, Gerssen Arjen, Diogène JorgeAbstract:Tetrodotoxin (TTX) is a potent neurotoxin responsible for many food poisoning incidents and some fatalities. Although mainly associated with the consumption of pufferfish, in recent years, TTX has been found in shellfish, particularly in Europe. In this work, a magnetic bead (MB)-based colorimetric immunoassay was applied to the detection of TTX in Pacific oysters (Crassostrea gigas), razor clams (Solen marginatus) and mussels (Mytilus galloprovincialis). Effective LODs (eLODs) for TTX of 1 μg/kg in oysters and razor clams and 3.3 μg/kg in mussels, significantly below the EFSA guidance threshold (44 μg/kg), were obtained. The strategy was applied to the analysis of naturally-contaminated Pacific oysters (Crassostrea gigas) and mussels (Mytilus edulis) from the Netherlands, and TTX was detected in all samples. The approach, which takes less than 1.5 h, proved to be useful as a rapid and simple method to detect TTX, support shellfish safety and protect consumers.
Emily Carrington - One of the best experts on this subject based on the ideXlab platform.
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investigating byssogenesis with proteomic analysis of byssus foot and mantle in Mytilus mussels by lc ms ms
Proteomics, 2020Co-Authors: Maxime Sansoucy, Rejean Tremblay, Emily Carrington, Isabelle Marcotte, Lekha SlenoAbstract:Mussel byssus represents a fascinating class of biological materials with a unique capacity to adhere onto virtually any solid surface. Proteins expressed in byssus, the byssal-producing organ (foot) as well as mantle tissue from Mytilus edulis or Mytilus californianus are analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The mantle is used as a control tissue to pinpoint unique proteins from the foot samples potentially involved in byssogenesis. This work represents an important step towards identifying biologically important proteins expressed in foot, as well as extending knowledge on the byssus proteome. Considering the minimal proteomics data of the studied species, this data also contributes to a more complete description of M. edulis and M. californianus proteomes.
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environmental post processing increases the adhesion strength of mussel byssus adhesive
Biofouling, 2018Co-Authors: Matthew N George, Emily CarringtonAbstract:Marine mussels (Mytilus trossulus) attach to a wide variety of surfaces underwater using a protein adhesive that is cured by the surrounding seawater environment. In this study, the influence of en...
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interspecies comparison of the mechanical properties and biochemical composition of byssal threads
The Journal of Experimental Biology, 2017Co-Authors: Zeineb Bouhlel, Rejean Tremblay, Emily Carrington, Bertrand Genard, Neilly Ibrahim, Jose M F Babarro, Aynur Lok, Augusto A V Flores, Christian Pellerin, Isabelle MarcotteAbstract:Several bivalve species produce byssus threads to provide attachment to substrates, with mechanical properties highly variable among species. Here, we examined the distal section of byssal threads produced by a range of bivalve species ( Mytilus edulis, Mytilus trossulus, Mytilus galloprovincialis, Mytilus californianus, Pinna nobilis, Perna perna, Xenostrobus securis , Brachidontes solisianus and Isognomon bicolor ) collected from different nearshore environments. Morphological and mechanical properties were measured, and biochemical analyses were performed. Multivariate redundancy analyses on mechanical properties revealed that byssal threads of M. californianus , M. galloprovincialis and P. nobilis have very distinct mechanical behaviors compared to the remaining species. Extensibility, strength and force were the main variables separating these species groups, which were highest for M. californianus and lowest for P. nobilis . Furthermore, the analysis of the amino acid composition revealed that I. bicolor and P. nobilis threads are significantly different from the other species, suggesting a different underlying structural strategy. Determination of metal contents showed that the individual concentration of inorganic elements varies but that the dominant elements are conserved between species. Altogether, this bivalve species comparison suggests some molecular bases for the biomechanical characteristics of byssal fibers that may reflect phylogenetic limitations.