The Experts below are selected from a list of 2781 Experts worldwide ranked by ideXlab platform
Javad Fasihi - One of the best experts on this subject based on the ideXlab platform.
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development of a localized surface plasmon resonance based gold Nanobiosensor for the determination of prolactin hormone in human serum
Analytical Biochemistry, 2016Co-Authors: Zahra Faridli, Mohamad Mahani, Masoud Torkzadehmahani, Javad FasihiAbstract:Abstract A localized surface plasmon resonance immunoassay has been developed to determine prolactin hormone in human serum samples. Gold nanoparticles were synthesized, and the probe was prepared by electrostatic adsorption of antibody on the surfaces of gold nanoparticles. The pH and the antibody-to-gold nanoparticle ratio, as the factors affecting the probe functions, were optimized. The constructed Nanobiosensor was characterized by dynamic light scattering. The sensor was applied for the determination of prolactin antigen concentration based on the amount of localized surface plasmon resonance peak shift. A linear dynamic range of 1–40 ng ml−1, a detection limit of 0.8 ng ml−1, and sensitivity of 10 pg ml−1 were obtained. Finally, the Nanobiosensor was applied for the determination of prolactin in human control serum sample.
Mohamad Mahani - One of the best experts on this subject based on the ideXlab platform.
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localized surface plasmon resonance based gold Nanobiosensor determination of thyroid stimulating hormone
Analytical Biochemistry, 2017Co-Authors: Afsaneh Salahvarzi, Mohamad Mahani, Masoud Torkzadehmahani, Reza AlizadehAbstract:Abstract An immunoassay method based on the peak shift of the localized surface plasmon resonance (LSPR) absorption maxima has been developed for the determination of the thyroid stimulating hormone (TSH) in human blood serum. The anti-TSH antibody was adsorbed on the synthesized gold nanoparticles by electrostatic forces. The efficiency of the Nanobiosensor was improved by optimizing the factors affecting the probe construction such as the pH and the antibody to gold nanoparticles ratio. Dynamic light scattering was applied for the characterization of the constructed probe. The amount of peak shift of the LSPR absorption maxima was selected as the basis for determination of TSH antigen. The linear dynamic range of 0.4–12.5 mIU L −1 and the calibration sensitivity of 1.71 L mIU −1 were obtained. The human control serum sample was analyzed for TSH by constructed Nanobiosensor and the acceptable results were obtained.
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development of a localized surface plasmon resonance based gold Nanobiosensor for the determination of prolactin hormone in human serum
Analytical Biochemistry, 2016Co-Authors: Zahra Faridli, Mohamad Mahani, Masoud Torkzadehmahani, Javad FasihiAbstract:Abstract A localized surface plasmon resonance immunoassay has been developed to determine prolactin hormone in human serum samples. Gold nanoparticles were synthesized, and the probe was prepared by electrostatic adsorption of antibody on the surfaces of gold nanoparticles. The pH and the antibody-to-gold nanoparticle ratio, as the factors affecting the probe functions, were optimized. The constructed Nanobiosensor was characterized by dynamic light scattering. The sensor was applied for the determination of prolactin antigen concentration based on the amount of localized surface plasmon resonance peak shift. A linear dynamic range of 1–40 ng ml−1, a detection limit of 0.8 ng ml−1, and sensitivity of 10 pg ml−1 were obtained. Finally, the Nanobiosensor was applied for the determination of prolactin in human control serum sample.
Masoud Torkzadehmahani - One of the best experts on this subject based on the ideXlab platform.
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localized surface plasmon resonance based gold Nanobiosensor determination of thyroid stimulating hormone
Analytical Biochemistry, 2017Co-Authors: Afsaneh Salahvarzi, Mohamad Mahani, Masoud Torkzadehmahani, Reza AlizadehAbstract:Abstract An immunoassay method based on the peak shift of the localized surface plasmon resonance (LSPR) absorption maxima has been developed for the determination of the thyroid stimulating hormone (TSH) in human blood serum. The anti-TSH antibody was adsorbed on the synthesized gold nanoparticles by electrostatic forces. The efficiency of the Nanobiosensor was improved by optimizing the factors affecting the probe construction such as the pH and the antibody to gold nanoparticles ratio. Dynamic light scattering was applied for the characterization of the constructed probe. The amount of peak shift of the LSPR absorption maxima was selected as the basis for determination of TSH antigen. The linear dynamic range of 0.4–12.5 mIU L −1 and the calibration sensitivity of 1.71 L mIU −1 were obtained. The human control serum sample was analyzed for TSH by constructed Nanobiosensor and the acceptable results were obtained.
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development of a localized surface plasmon resonance based gold Nanobiosensor for the determination of prolactin hormone in human serum
Analytical Biochemistry, 2016Co-Authors: Zahra Faridli, Mohamad Mahani, Masoud Torkzadehmahani, Javad FasihiAbstract:Abstract A localized surface plasmon resonance immunoassay has been developed to determine prolactin hormone in human serum samples. Gold nanoparticles were synthesized, and the probe was prepared by electrostatic adsorption of antibody on the surfaces of gold nanoparticles. The pH and the antibody-to-gold nanoparticle ratio, as the factors affecting the probe functions, were optimized. The constructed Nanobiosensor was characterized by dynamic light scattering. The sensor was applied for the determination of prolactin antigen concentration based on the amount of localized surface plasmon resonance peak shift. A linear dynamic range of 1–40 ng ml−1, a detection limit of 0.8 ng ml−1, and sensitivity of 10 pg ml−1 were obtained. Finally, the Nanobiosensor was applied for the determination of prolactin in human control serum sample.
Reza Alizadeh - One of the best experts on this subject based on the ideXlab platform.
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localized surface plasmon resonance based gold Nanobiosensor determination of thyroid stimulating hormone
Analytical Biochemistry, 2017Co-Authors: Afsaneh Salahvarzi, Mohamad Mahani, Masoud Torkzadehmahani, Reza AlizadehAbstract:Abstract An immunoassay method based on the peak shift of the localized surface plasmon resonance (LSPR) absorption maxima has been developed for the determination of the thyroid stimulating hormone (TSH) in human blood serum. The anti-TSH antibody was adsorbed on the synthesized gold nanoparticles by electrostatic forces. The efficiency of the Nanobiosensor was improved by optimizing the factors affecting the probe construction such as the pH and the antibody to gold nanoparticles ratio. Dynamic light scattering was applied for the characterization of the constructed probe. The amount of peak shift of the LSPR absorption maxima was selected as the basis for determination of TSH antigen. The linear dynamic range of 0.4–12.5 mIU L −1 and the calibration sensitivity of 1.71 L mIU −1 were obtained. The human control serum sample was analyzed for TSH by constructed Nanobiosensor and the acceptable results were obtained.
Zahra Faridli - One of the best experts on this subject based on the ideXlab platform.
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development of a localized surface plasmon resonance based gold Nanobiosensor for the determination of prolactin hormone in human serum
Analytical Biochemistry, 2016Co-Authors: Zahra Faridli, Mohamad Mahani, Masoud Torkzadehmahani, Javad FasihiAbstract:Abstract A localized surface plasmon resonance immunoassay has been developed to determine prolactin hormone in human serum samples. Gold nanoparticles were synthesized, and the probe was prepared by electrostatic adsorption of antibody on the surfaces of gold nanoparticles. The pH and the antibody-to-gold nanoparticle ratio, as the factors affecting the probe functions, were optimized. The constructed Nanobiosensor was characterized by dynamic light scattering. The sensor was applied for the determination of prolactin antigen concentration based on the amount of localized surface plasmon resonance peak shift. A linear dynamic range of 1–40 ng ml−1, a detection limit of 0.8 ng ml−1, and sensitivity of 10 pg ml−1 were obtained. Finally, the Nanobiosensor was applied for the determination of prolactin in human control serum sample.
