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Ihab Ghabeish - One of the best experts on this subject based on the ideXlab platform.
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impact of flavonoids against woolly apple aphid eriosoma lanigerum hausmann and its sole parasitoid aphelinus mali hald
The Journal of Agricultural Science, 2012Co-Authors: Mazen A Ateyyat, Saeid Aburomman, Mohmmad Abudarwish, Ihab GhabeishAbstract:Cut-shoot bioassay test was used to study the significance of three flavonoids as aphicides against the woolly apple aphid (WAA), Eriosoma lanigerum (Hausmann). The used flavonoids were two flavanols that are quercetin dehydrate and rutin hydrate, but rutin hydrate is a glycoside of quercetin dehydrate. In addition to one flavanone that was Naringine. These flavonoids were used at three concentrations; 100 ppm, 1000 ppm and 10,000 ppm. Results showed that the three tested flavonoids were active as aphicides against the target species and that mortality to nymphs was higher than that obtained against apterous adults. Increasing the concentration of the flavonoids resulted in a remarkable increase in nymphs mortality. However, rutin hydrate is more toxic to WAA than quercetin dehydrate and Naringin. The three flavonoids had slight effect on the sole parasitoid of WAA, Aphelinus mali compared with effect caused by imodacloprid insecticide. Quercetin dehydrate, rutin hydrate and Naringine can be used as botanical insecticides and incorporated into integrated management programs of the aphid.
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impact of flavonoids against woolly apple aphid eriosoma lanigerum hausmann and its sole parasitoid aphelinus mali hald
The Journal of Agricultural Science, 2012Co-Authors: Mazen A Ateyyat, Saeid Aburomman, Mohmmad Abudarwish, Ihab GhabeishAbstract:Cut-shoot bioassay test was used to study the significance of three flavonoids as aphicides against the woolly apple aphid (WAA), Eriosoma lanigerum (Hausmann). The used flavonoids were two flavanols that are quercetin dehydrate and rutin hydrate, but rutin hydrate is a glycoside of quercetin dehydrate. In addition to one flavanone that was Naringine. These flavonoids were used at three concentrations; 100 ppm, 1000 ppm and 10,000 ppm. Results showed that the three tested flavonoids were active as aphicides against the target species and that mortality to nymphs was higher than that obtained against apterous adults. Increasing the concentration of the flavonoids resulted in a remarkable increase in nymphs mortality. However, rutin hydrate is more toxic to WAA than quercetin dehydrate and Naringin. The three flavonoids had slight effect on the sole parasitoid of WAA, Aphelinus mali compared with effect caused by imodacloprid insecticide. Quercetin dehydrate, rutin hydrate and Naringine can be used as botanical insecticides and incorporated into integrated management programs of the aphid.
Myungsook Choi - One of the best experts on this subject based on the ideXlab platform.
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antihypercholesterolemic property of Naringin alters plasma and tissue lipids cholesterol regulating enzymes fecal sterol and tissue morphology in rabbits
Clinical Nutrition, 2004Co-Authors: Seonmin Jeon, Yong Bok Park, Myungsook ChoiAbstract:Abstract Background & aims: Hyperlipidemia is a major risk factor for cardiovascular diseases. This study was designed to confirm the hypocholesterolemic role of Naringin. Methods: Male rabbits were fed 0.5% high-cholesterol diet or high-cholesterol diet supplemented with either 0.05% Naringin or 0.03% lovastatin for 8 weeks. Results: The Naringin and lovastatin supplements significantly lowered plasma total- and LDL-cholesterol and hepatic lipids levels, while significantly increasing HDL-C/total-C ratio compared to the control group. Hepatic 3-hydroxy-3-methylglutaryl CoA reductase and acyl-CoA: cholesterol acyltransferase activities were significantly higher and lower, respectively, in both supplemented groups than the control group. Total fecal sterol content was significantly increased in lovastatin and especially Naringin group. In histopathological analyses, only control group exhibited hepatic lipid droplets, cardiac adipocyte infiltration and slight damage of endothelial lining in aortic wall, but two supplements retarded these atherogenic signs. Conclusion: It would appear that both Naringin and lovastatin contributed to hypocholesterolemic action via down-regulated ACAT activity and higher excretion of fecal sterols in response to high-cholesterol feeding. Also, Naringin supplement seemed to preserve tissue morphology from damages induced by high cholesterol diet.
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Naringin supplementation lowers plasma lipids and enhances erythrocyte antioxidant enzyme activities in hypercholesterolemic subjects
Clinical Nutrition, 2003Co-Authors: Un Ju Jung, Mikyung Lee, Hye Jin Kim, J S Lee, H O Kim, E J Park, H K Kim, Taesook Jeong, Myungsook ChoiAbstract:Abstract Background and aims: Preliminary studies have shown that Naringin has a potent lipid-lowering effect and antioxidant capacity in high-cholesterol diet fed animals. Accordingly, the present study was conducted to investigate the effect of Naringin on hypercholesterolemic subjects. Methods: A hypercholesterolemic group ( n =30) and healthy control group ( n =30) were established based on the plasma cholesterol levels in the subjects, then all subjects received Naringin (400mg/capsule/day) with regular meals for a period of 8 weeks. Results: In the hypercholesterolemic subjects, Naringin supplementation was found to lower the plasma total cholesterol by 14% and low-density lipoprotein cholesterol concentrations by 17%, while the plasma triglyceride and high-density lipoprotein cholesterol concentrations remained unaffected. The apolipoprotein B levels in the hypercholesterolemic subjects were significantly lowered after Naringin treatment, yet no change was observed in the apolipoprotein A-1 levels. The erythrocyte superoxide dismutase and catalase activities in the hypercholesterolemic group were significantly increased, whereas the glutathione peroxidase activity and plasma TBARS levels were not different from the baseline measurements. Meanwhile, Naringin supplementation had no affect on plasma lipids, apolipoproteins, and TBARS levels or antioxidant enzyme activities in the control group. Conclusions: Therefore, these data suggest that Naringin may play an important role in lowering plasma cholesterol and regulating the antioxidant capacity in hypercholesterolemic subjects.
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role of Naringin supplement in regulation of lipid and ethanol metabolism in rats
Life Sciences, 2003Co-Authors: Hyunju Seo, Kyushik Jeong, Mikyung Lee, Yong Bok Park, Un Ju Jung, Hye Jin Kim, Myungsook ChoiAbstract:The current study was performed to investigate the effect of Naringin supplements on the alcohol, lipid, and antioxidant metabolism in ethanol-treated rats. Male Sprague-Dawley rats were randomly divided into six groups (n = 10) based on six dietary categories: ethanol and Naringin-free, ethanol (50 g/L) plus low-Naringin (0.05 g/L), ethanol plus high-Naringin (0.125 g/L), and three corresponding pair-fed groups. The pair-fed control rats received an isocaloric diet containing dextrin-maltose instead of ethanol for 5 wks. Among the ethanol treated groups, the Naringin supplements significantly lowered the plasma ethanol concentration with a simultaneous increase in the ADH and/or ALDH activities. However, among the ethanol-treated groups, Naringin supplementation resulted in a significant decrease in the hepatic triglycerides and plasma and hepatic total cholesterol compared to that in the Naringin-free group. Naringin supplementation significantly increased the HDL-cholesterol and HDL-C/total-C ratio, while lowering the AI value among the ethanol-treated groups. Hepatic lipid accumulation was also significantly reduced in the Naringin-supplemented groups compared to the Naringin-free group among the ethanol-treated groups, while no differences were found among the pair-fed groups. Among the ethanol-treated groups, the low-Naringin supplementation resulted in a significant decrease in the levels of plasma and hepatic TBARS, whereas it resulted in higher SOD and GSH-Px activities and gluthathion levels in the liver. Accordingly, Naringin would appear to contribute to alleviating the adverse effect of ethanol ingestion by enhancing the ethanol and lipid metabolism as well as the hepatic antioxidant defense system.
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comparison of antioxidant effects of Naringin and probucol in cholesterol fed rabbits
Clinica Chimica Acta, 2002Co-Authors: Seonmin Jeon, Yong Bok Park, Taesook Jeong, Song Hae Bok, Moon Kyoo Jang, Yeon Hee Kim, Kyung Tak Nam, Myungsook ChoiAbstract:Abstract Background: Due to the strong evidence on the involvement of active oxygen species in a variety of disorders, the role of antioxidants against oxidative stress has recently received increased attention. Methods: Twenty male rabbits were served a high-cholesterol (HC, 5 g/kg diet) diet or high-cholesterol diet supplemented with Naringin (0.5 g/kg diet) or probucol (0.5 g/kg diet) for 8 weeks to compare the antioxidative effects of the citrus bioflavonoid (Naringin) and antioxidative cholesterol-lowering drug (probucol). Results: The plasma thiobarbituric acid-reactive substances (TBARS) concentration was not significantly different between the groups, whereas the hepatic TBARS concentration was significantly lower in the probucol group than in both normal and HC control or Naringin group. Probucol and Naringin supplementation led to an increase in the hepatic superoxide dismutase (SOD) and catalase (CAT) activities, and a decrease in the hepatic mitochondrial hydrogen peroxide (H2O2) content compared to the HC-control group. However, there was no difference in the cytosolic H2O2 content or cytosolic glutathion peroxidase (GSH-Px) activity in the liver between the groups. Both Naringin and probucol supplements significantly increased the plasma vitamin E concentration compared to the HC-control group. As regards the antioxidant enzyme gene expressions, Naringin significantly increased the expression of three antioxidant enzyme mRNAs compared to the HC-control group, whereas probucol significantly increased the only SOD mRNA expression. Conclusions: The probucol supplement was very potent in the antioxidative defense system, whereas Naringin exhibited a comparable antioxidant capacity based on increasing the gene expressions in the antioxidant enzymes, while also increasing the hepatic SOD and CAT activities, sparing plasma vitamin E, and decreasing the hepatic mitochondrial H2O2 content.
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antioxidative activity of Naringin and lovastatin in high cholesterol fed rabbits
Life Sciences, 2001Co-Authors: Seonmin Jeon, Mikyung Lee, Yong Bok Park, Song Hae Bok, Moon Kyoo Jang, Kyung Tak Nam, Soon Jae Rhee, Myungsook ChoiAbstract:The consumption of a cholesterol-enriched diet increases the degree of lipid peroxidation, which is one of the early processes of atherosclerosis. The aim of this trial was to determine the antioxidative effects of the citrus bioflavonoid, Naringin, a potent cholesterol-lowering agent, compared to the cholesterol-lowering drug, lovastatin, in rabbits fed a high cholesterol diet. Male rabbits were served a high-cholesterol (0.5%, w/w) diet or high-cholesterol diet supplemented with either Naringin (0.5% cholesterol, 0.05% Naringin, w/w) or lovastatin (0.5% cholesterol, 0.03% lovastatin, w/w) for 8 weeks to determine the plasma and hepatic lipid peroxide, plasma vitamin A and E levels, and hepatic hydrogen peroxide levels, along with the hepatic antioxidant enzyme activities and gene expressions. Only the lovastatin group showed significantly lower plasma and hepatic lipid peroxide levels compared to the control group. The Naringin supplementation significantly increased the activities of both hepatic SOD and catalase by 33% and 20%, respectively, whereas the lovastatin supplementation only increased the catalase activity by 23% compared to control group. There was no difference in the GSH-Px activities between the various groups. Content of H2O2 in hepatic mitochondria was significantly lower in groups supplemented with lovastatin and Naringin than in control group. However, there was no difference in cytosolic H2O2 content in liver between groups. The concentration of plasma vitamin E was significantly increased by the Naringin supplementation. When comparing the antioxidant enzyme gene expression, the mRNA expression of SOD, catalase and GSH-Px was significantly up-regulated in the Naringin-supplemented group. Accordingly, these results would appear to indicate that Naringin, a citrus bioflavonoid, plays an important role in regulating antioxidative capacities by increasing the SOD and catalase activities, up-regulating the gene expressions of SOD, catalase, and GSH-Px, and protecting the plasma vitamin E. In contrast, lovastatin exhibited an inhibitory effect on the plasma and hepatic lipid peroxidation and increased the hepatic catalase activity in high-cholesterol fed rabbits.
Mazen A Ateyyat - One of the best experts on this subject based on the ideXlab platform.
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impact of flavonoids against woolly apple aphid eriosoma lanigerum hausmann and its sole parasitoid aphelinus mali hald
The Journal of Agricultural Science, 2012Co-Authors: Mazen A Ateyyat, Saeid Aburomman, Mohmmad Abudarwish, Ihab GhabeishAbstract:Cut-shoot bioassay test was used to study the significance of three flavonoids as aphicides against the woolly apple aphid (WAA), Eriosoma lanigerum (Hausmann). The used flavonoids were two flavanols that are quercetin dehydrate and rutin hydrate, but rutin hydrate is a glycoside of quercetin dehydrate. In addition to one flavanone that was Naringine. These flavonoids were used at three concentrations; 100 ppm, 1000 ppm and 10,000 ppm. Results showed that the three tested flavonoids were active as aphicides against the target species and that mortality to nymphs was higher than that obtained against apterous adults. Increasing the concentration of the flavonoids resulted in a remarkable increase in nymphs mortality. However, rutin hydrate is more toxic to WAA than quercetin dehydrate and Naringin. The three flavonoids had slight effect on the sole parasitoid of WAA, Aphelinus mali compared with effect caused by imodacloprid insecticide. Quercetin dehydrate, rutin hydrate and Naringine can be used as botanical insecticides and incorporated into integrated management programs of the aphid.
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impact of flavonoids against woolly apple aphid eriosoma lanigerum hausmann and its sole parasitoid aphelinus mali hald
The Journal of Agricultural Science, 2012Co-Authors: Mazen A Ateyyat, Saeid Aburomman, Mohmmad Abudarwish, Ihab GhabeishAbstract:Cut-shoot bioassay test was used to study the significance of three flavonoids as aphicides against the woolly apple aphid (WAA), Eriosoma lanigerum (Hausmann). The used flavonoids were two flavanols that are quercetin dehydrate and rutin hydrate, but rutin hydrate is a glycoside of quercetin dehydrate. In addition to one flavanone that was Naringine. These flavonoids were used at three concentrations; 100 ppm, 1000 ppm and 10,000 ppm. Results showed that the three tested flavonoids were active as aphicides against the target species and that mortality to nymphs was higher than that obtained against apterous adults. Increasing the concentration of the flavonoids resulted in a remarkable increase in nymphs mortality. However, rutin hydrate is more toxic to WAA than quercetin dehydrate and Naringin. The three flavonoids had slight effect on the sole parasitoid of WAA, Aphelinus mali compared with effect caused by imodacloprid insecticide. Quercetin dehydrate, rutin hydrate and Naringine can be used as botanical insecticides and incorporated into integrated management programs of the aphid.
Oluwakemi A Rotimi - One of the best experts on this subject based on the ideXlab platform.
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Naringin enhances reverse cholesterol transport in high fat low streptozocin induced diabetic rats
Biomedicine & Pharmacotherapy, 2018Co-Authors: Solomon Oladapo Rotimi, Isaacson Adelani, Goodness Esther Bankole, Oluwakemi A RotimiAbstract:Naringin, a citrus-derived flavonoid with antihyperglycemic, antihyperlipidemic, and antioxidant properties, is reported to be a useful nutraceutical in the management of diabetes and its complications. This study investigated the mechanism of antiatherogenic properties of Naringin in type 2 diabetes (T2DM) using high fat-low streptozocin rat model of T2DM. Rats were treated daily with 50, 100 and 200 mg/kg Naringin orally for 21days. Levels of biomarkers of T2DM, lipid profile and activity of paraoxonase (PON) were assayed spectrophotometrically. The levels of expression of hepatic 3-hydroxy-3-methyl-glutaryl-CoA reductase (Hmgcr), scavenger receptor class B member 1 (Scarb1), aryl hydrocarbon receptor (Ahr), hepatic Lipase (Lipc), and lecithin-cholesterol acyltransferase (Lcat) were assessed using relative reverse transcriptase polymerase chain reaction technique. Naringin treatment resulted in a dose-dependent significant (p < 0.05) decrease in the levels of plasma cholesterol and triglyceride from 84.84 ± 1.62 to 55.59 ± 1.50 mg/dL and 123.03 ± 15.11 to 55.00 ± 0.86 mg/dL, respectively, at 200 mg/kg Naringin. In the liver, Scarb1 and Ahr were significantly (p < 0.05) upregulated at 200 mg/kg Naringin while Lipc and Lcat were significantly (p < 0.05) upregulated by 50 mg/kg Naringin. T2DM-induced decrease in PON activities in the plasma, liver and HDL was significantly (p < 0.05) reversed by 200 mg/kg Naringin treatment. These genes play critical roles in reverse cholesterol transport and hence our results showed that the antiatherogenic property of Naringin in T2DM involves enhancement of reverse cholesterol transport and PON activity.
Cristiani Barros Da Silva Rosa - One of the best experts on this subject based on the ideXlab platform.
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Nariginase of Aspergillus niger: Production optimization by response surface methodology and utilisation of ultrasound for extractionNaringinase de Aspergillus niger: Otimização da produção por metodologia de superfície de resposta e uso do ultrassom
Universidade Estadual de Londrina, 2011Co-Authors: Maria Antonia Pedrine Colabone Celligoi, João Batista Buzato, Dionizio Borsato, Cristiani Barros Da Silva RosaAbstract:Brazil is the world's largest producer of orange and concentrated juice for export. Concentrated juice with high levels of Naringin has excessive bitterness, which reduces the quality and value on the market. The debittering can be obtained by using Naringinase, an enzymatic complex that degrades Naringin. This study reports the production of Naringinase by Aspergillus niger 426 utilizing both sugar cane molasses as carbon source and yeast extract as nitrogen source. Naringin was used as inducer. Five nitrogen sources were studied and yeast extract was found as the best one as 225.6 mU/mL of Naringinase activity at 120 hours of fermentation was achivied. For optimization of Naringinase production it was applied response-surface methodology, with 22 incomplete factorial design. An activity value of 354.26 mU/mL of Naringinase was achivied when as independent variables yeast extract (14.0g/L) and Naringin (0.2g/L) were used. When applied ultrasound waves at 20 kHz of intensity for 2 minutes in the fermented broth, the activity reached the highest value of 473.6 mU/mL. Thus, Naringinase, this enzyme of great potential for biotechnological applications, has its production increased by using statistical design and ultrasound.O Brasil é o maior produtor mundial de laranja e de suco concentrado destinado à exportação. Sucos cítricos concentrados para exportação com níveis elevados de Naringina são excessivamente amargos, o que reduz a qualidade e o valor comercial do produto. A redução do amargor pode ser obtida pela Naringinase, um complexo enzimático que degrada a Naringina. Neste trabalho Aspergillus niger 426 foi usado como produtor de Naringinase utilizando matérias-prima da agroindústria, melaço como fonte de carbono e extrato de levedura como fonte de nitrogênio. Naringina foi usada como indutor. Dentre as cinco fontes de nitrogênio pesquisadas, extrato de levedura apresentou o melhor resultado de 225,6 mU/mL de Naringinase em 120 horas de cultivo. Para otimização da produção de Naringinase, foi aplicada a metodologia de superfície de resposta, com planejamento fatorial incompleto 22, obtendo 354,26 mU/mL de atividade de Naringinase, tendo como variáveis independentes o extrato de levedura (14,0g/L) e Naringina (0,2g/L). Quando aplicadas ondas de ultrassom com intensidade de 20 kHz por 2 minutos na cultura, a atividade de Naringinase atingiu o valor máximo de 473,6 mU/mL. Assim, a Naringinase, enzima de grande potencial de aplicação biotecnológica, teve a produção aumentada pelo uso do planejamento estatístico e ultrassom
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Naringinase de Aspergillus niger : otimização da produção por metodologia de superfície de resposta e uso do ultrassom para extração
Universidade Estadual de Londrina. Centro de Ciências Exatas. Programa de Pós-Graduação em Biotecnologia., 2009Co-Authors: Cristiani Barros Da Silva RosaAbstract:O Brasil é o maior produtor mundial de laranja e de suco concentrado destinado à exportação. Sucos cítricos concentrados para exportação com níveis elevados de Naringina são excessivamente amargos, o que reduz a qualidade e o valor comercial do produto. A redução do amargor pode ser obtida pela Naringinase, um complexo enzimático que degrada a Naringina. Neste trabalho Aspergillus niger 426 foi usado como produtor de Naringinase utilizando matérias-prima da agroindústria, melaço como fonte de carbono e extrato de levedura como fonte de nitrogênio. Naringina foi usada como indutor. Dentre as cinco fontes de nitrogênio pesquisadas, extrato de levedura apresentou o melhor resultado de 225,6 mU/mL de Naringinase em 120 horas de cultivo. Para otimização da produção de Naringinase, foi aplicada a metodologia de superfície de resposta, com planejamento fatorial incompleto 22, obtendo 354,26 mU/mL de atividade de Naringinase, tendo como variáveis independentes o extrato de levedura (14,0g/L) e Naringina (0,2g/L). Quando aplicadas ondas de ultrassom com intensidade de 20 kHz por 2 minutos na cultura, a atividade de Naringinase atingiu o valor máximo de 473,6 mU/mL. Assim, a Naringinase, enzima de grande potencial de aplicação biotecnológica, teve a produção aumentada pelo uso do planejamento estatístico e ultrassom.Brazil is the world's largest producer of orange and concentrated juice for export. Concentrated juice with high levels of Naringin has excessive bitterness, which reduces the quality and value on the market. The debittering can be obtained by using Naringinase, an enzymatic complex that degrades Naringin. This study reports the production of Naringinase by Aspergillus niger 426 utilizing both sugar cane molasses as carbon source and yeast extract as nitrogen source. Naringin was used as inducer. Five nitrogen sources were studied and yeast extract was found as the best one as 225.6 mU/mL of Naringinase activity at 120 hours of fermentation was achivied. For optimization of Naringinase production it was applied response-surface methodology, with 22 incomplete factorial design. An activity value of 354.26 mU/mL of Naringinase was achivied when as independent variables yeast extract (14.0g/L) and Naringin (0.2g/L) were used. When applied ultrasound waves at 20 kHz of intensity for 2 minutes in the fermented broth, the activity reached the highest value of 473.6 mU/mL. Thus, Naringinase, this enzyme of great potential for biotechnological applications, has its production increased by using statistical design and ultrasound
