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S R Durham - One of the best experts on this subject based on the ideXlab platform.
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influence of seasonal exposure to grass pollen on local and peripheral blood ige repertoires in patients with allergic rhinitis
The Journal of Allergy and Clinical Immunology, 2014Co-Authors: S R Durham, Yuchang B Wu, Louisa K James, Jason Vander A Heiden, Mohamed Uduman, Steven H Kleinstein, David Glyn KiplingAbstract:Background Previous studies of immunoglobulin gene sequences in patients with allergic diseases using low-throughput Sanger sequencing have limited the analytic depth for characterization of IgE repertoires. Objectives We used a high-throughput, next-generation sequencing approach to characterize immunoglobulin heavy-chain gene (IGH) repertoires in patients with seasonal allergic rhinitis (AR) with the aim of better understanding the underlying disease mechanisms. Methods IGH sequences in matched peripheral blood and Nasal Biopsy specimens from nonallergic healthy control subjects (n = 3) and patients with grass pollen–related AR taken in season (n = 3) or out of season (n = 4) were amplified and pyrosequenced on the 454 GS FLX+ System. Results A total of 97,610 IGH (including 8,135 IgE) sequences were analyzed. Use of immunoglobulin heavy-chain variable region gene families 1 (IGHV1) and 5 (IGHV5) was higher in IgE clonotypic repertoires compared with other antibody classes independent of atopic status. IgE repertoires measured inside the grass pollen season were more diverse and more mutated (particularly in the Biopsy specimens) and had more evidence of antigen-driven selection compared with those taken outside of the pollen season or from healthy control subjects. Clonal relatedness was observed for IgE between the blood and Nasal Biopsy specimens. Furthermore in patients with AR, but not healthy control subjects, we found clonal relatedness between IgE and IgG classes. Conclusion This is the first report that exploits next-generation sequencing to determine local and peripheral blood IGH repertoires in patients with respiratory allergic disease. We demonstrate that natural pollen exposure was associated with changes in IgE repertoires that were suggestive of ongoing germinal center reactions. Furthermore, these changes were more often apparent in Nasal Biopsy specimens compared with peripheral blood and in patients with AR compared with healthy control subjects.
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Influence of seasonal exposure to grass pollen on local and peripheral blood IgE repertoires in patients with allergic rhinitis.
The Journal of allergy and clinical immunology, 2014Co-Authors: Louisa K James, S R Durham, Mohamed Uduman, Steven H Kleinstein, David Glyn Kipling, Jason A. Vander Heiden, Hannah J. GouldAbstract:Background Previous studies of immunoglobulin gene sequences in patients with allergic diseases using low-throughput Sanger sequencing have limited the analytic depth for characterization of IgE repertoires. Objectives We used a high-throughput, next-generation sequencing approach to characterize immunoglobulin heavy-chain gene (IGH) repertoires in patients with seasonal allergic rhinitis (AR) with the aim of better understanding the underlying disease mechanisms. Methods IGH sequences in matched peripheral blood and Nasal Biopsy specimens from nonallergic healthy control subjects (n = 3) and patients with grass pollen–related AR taken in season (n = 3) or out of season (n = 4) were amplified and pyrosequenced on the 454 GS FLX+ System. Results A total of 97,610 IGH (including 8,135 IgE) sequences were analyzed. Use of immunoglobulin heavy-chain variable region gene families 1 (IGHV1) and 5 (IGHV5) was higher in IgE clonotypic repertoires compared with other antibody classes independent of atopic status. IgE repertoires measured inside the grass pollen season were more diverse and more mutated (particularly in the Biopsy specimens) and had more evidence of antigen-driven selection compared with those taken outside of the pollen season or from healthy control subjects. Clonal relatedness was observed for IgE between the blood and Nasal Biopsy specimens. Furthermore in patients with AR, but not healthy control subjects, we found clonal relatedness between IgE and IgG classes. Conclusion This is the first report that exploits next-generation sequencing to determine local and peripheral blood IGH repertoires in patients with respiratory allergic disease. We demonstrate that natural pollen exposure was associated with changes in IgE repertoires that were suggestive of ongoing germinal center reactions. Furthermore, these changes were more often apparent in Nasal Biopsy specimens compared with peripheral blood and in patients with AR compared with healthy control subjects.
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Objective monitoring of Nasal airway inflammation in rhinitis
The Journal of Allergy and Clinical Immunology, 2005Co-Authors: Peter H. Howarth, M R Jacobson, S R Durham, Carl G. A. Persson, Eli O. Meltzer, Philip E. SilkoffAbstract:Allergic rhinitis is an inflammatory Nasal disorder in which a range of different cells participates. A variety of approaches has been used to monitor Nasal inflammation objectively to investigate disease processes and to evaluate the effect of therapeutic intervention. These approaches include Nasal lavage, Nasal cytology, and Nasal Biopsy, together with the more recently established measurement of Nasal nitric oxide (NO) concentration. Although all provide information about Nasal mucosal inflammation, the extent of information that can be obtained by each approach, the ease of sampling, and the complexity of sample handling differ. Such considerations influence the choice of approach when measurement of Nasal inflammation is to be an objective outcome parameter in a clinical trial. In addition, the choice of approach is also determined by the questions or hypotheses that are to be addressed. Nasal lavage is simple and rapid to perform, is well tolerated, and provides a sample that can provide information about luminal cell recruitment, cell activation, and plasma protein extravasation. Nasal cytology involves sampling and recovering mucosal surface cells. It is also easy to perform and is well tolerated in general, although some find that the procedure causes a transient unpleasant sensation. A differential cell count from the sample provides information about relative cell populations. Both Nasal lavage and Nasal cytology are readily applicable to clinical trials. Nasal cytology sample handling is easier, but Nasal lavage offers the advantage of providing considerably greater information from the sample. Nasal Biopsy is a considerably more invasive procedure and requires expertise not only in tissue sampling but also in Biopsy processing. Therefore, it is applicable only in specialist centers. However, Nasal Biopsy is the only sampling technique that directly informs about tissue cellular events, although these may be implied, in part from the other sampling approaches. Tissue specimens can be used to evaluate both protein and gene expression. Measurement of Nasal NO involves expensive equipment but provides an instantaneous result, unlike the other approaches, all of which require sample processing and analysis. Recommendations for standardization of measurement have been made, and measures are considered in part to reflect allergic inflammation within the Nasal mucosa. The limitations of Nasal NO are that it reflects only a certain aspect of allergic mucosal inflammation, and that because a proportion of Nasally measured NO is derived from the sinuses under normal circumstances, Nasal NO is not specific for Nasal disease. The high contribution from the sinus mucosa limits the discriminatory ability of Nasal NO to reflect Nasal tissue-specific alterations. The incorporation of measures of Nasal inflammation in clinical trials has distinguished anti-inflammatory therapy from symptomatic therapy and has the potential to provide information about the efficacy of novel therapies for allergic rhinitis.
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effect of topical corticosteroids on seasonal increases in epithelial eosinophils and mast cells in allergic rhinitis a comparison of Nasal brush and Biopsy methods
Clinical & Experimental Allergy, 1999Co-Authors: M R Jacobson, S Juliusson, O Lowhagen, B Balder, S R DurhamAbstract:Background Nasal brushing and Nasal Biopsy are well-tolerated sampling techniques. Seasonal grass pollen-induced rhinitis is characterized by epithelial mast cell infiltration and seasonal increases in both epithelial and sub-mucosal eosinophils. Objective To compare the ability of the Nasal brush and Nasal Biopsy techniques to detect natural seasonal increases in eosinophils and mast cells, and to assess the influence of topical corticosteroid. Methods Nasal brush samples and Nasal biopsies were collected from 46 grass pollen-sensitive seasonal rhinitis patients before the grass pollen season and at the peak of the pollen season following 6 weeks' treatment with either fluticasone propionate aqueous Nasal spray (200 μg, twice daily) or placebo Nasal spray. Results Placebo patients showed seasonal increases in epithelial eosinophils both with Nasal brushing (P < 0.0001) and Biopsy (P < 0.001). Epithelial mast cell numbers also increased during the pollen season as detectable by brushing (P < 0.0001) and Biopsy (P < 0.03). Changes in cell numbers measured by Nasal brushing correlated with those observed with Nasal Biopsy, both for eosinophils and mast cells (P < 0.05). Sub-mucosal eosinophils but not mast cells also increased during the pollen season (P < 0.002). Nasal brushing and Biopsy revealed that fluticasone treatment inhibited seasonal increases in epithelial eosinophils (P < 0.00001) and epithelial infiltration by mast cells (Nasal brushing P < 0.00001 and Nasal Biopsy P < 0.01). Fluticasone also inhibited seasonal increases in sub-mucosal eosinophils (P < 0.001) and significantly reduced Nasal symptoms (P < 0.001). Conclusion Nasal brushing harvests sufficient inflammatory cells from the surface of the Nasal mucosa to be used in lieu of Nasal biopsies in observation of the effect of drugs on the Nasal epithelium.
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Effect of topical corticosteroids on seasonal increases in epithelial eosinophils and mast cells in allergic rhinitis: a comparison of Nasal brush and Biopsy methods
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 1999Co-Authors: M R Jacobson, S Juliusson, O Lowhagen, B Balder, A. B. Kay, S R DurhamAbstract:Background Nasal brushing and Nasal Biopsy are well-tolerated sampling techniques. Seasonal grass pollen-induced rhinitis is characterized by epithelial mast cell infiltration and seasonal increases in both epithelial and sub-mucosal eosinophils. Objective To compare the ability of the Nasal brush and Nasal Biopsy techniques to detect natural seasonal increases in eosinophils and mast cells, and to assess the influence of topical corticosteroid. Methods Nasal brush samples and Nasal biopsies were collected from 46 grass pollen-sensitive seasonal rhinitis patients before the grass pollen season and at the peak of the pollen season following 6 weeks' treatment with either fluticasone propionate aqueous Nasal spray (200 μg, twice daily) or placebo Nasal spray. Results Placebo patients showed seasonal increases in epithelial eosinophils both with Nasal brushing (P
I S Mackay - One of the best experts on this subject based on the ideXlab platform.
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assessment by Nasal Biopsy of long term use of mometasone furoate aqueous Nasal spray nasonex in the treatment of perennial rhinitis
Otolaryngology-Head and Neck Surgery, 1998Co-Authors: E Minshall, Omar Ghaffar, L Cameron, F Obrien, H Quinn, Julian Rowejones, R J Davies, A Prior, Valerie J Lund, I S MackayAbstract:Abstract Allergic rhinitis is associated with specific histopathologic changes in the Nasal mucosa including squamous metaplasia and local eosinophilia. Previous studies have shown that mometasone furoate aqueous Nasal spray is effective and well tolerated in reducing perennial rhinitis and seasonal allergic rhinitis symptoms. We undertook a multicenter, open-label study to evaluate, by Nasal Biopsy, the tissue changes associated with mometasone furoate use (200 μg/day) during a 12-month treatment period in patients with perennial rhinitis. Of the 69 patients enrolled in the study, 52 completed all 12 months of treatment. Nasal Biopsy specimens obtained from patients at baseline and after treatment were evaluated in a blinded fashion by computerized image analysis, qualitative histologic examination, and immunocytochemistry. Morphologic examination of Nasal Biopsy specimens showed a decrease in focal metaplasia, no change in epithelial thickness, and no sign of atrophy after treatment with mometasone furoate. Immunocytochemical analyses of Nasal Biopsy specimens obtained before and after treatment revealed a significant decrease in major basic protein–positive eosinophils and tryptase-positive mast cells in the epithelium and lamina propria after treatment. Mometasone furoate appeared to attenuate the inflammatory process by reducing the extent of inflammatory cell infiltration, particularly of eosinophils. This study demonstrated that long-term administration of mometasone furoate is not associated with adverse tissue changes in the Nasal mucosa of patients with perennial rhinitis. (Otolaryngol Head Neck Surg 1998;118:648-54.)
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Assessment by Nasal Biopsy of long-term use of mometasone furoate aqueous Nasal spray (Nasonex) in the treatment of perennial rhinitis.
Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 1998Co-Authors: E Minshall, Omar Ghaffar, L Cameron, H Quinn, R J Davies, A Prior, Valerie J Lund, F. O'brien, Julian Rowe-jones, I S MackayAbstract:Allergic rhinitis is associated with specific histopathologic changes in the Nasal mucosa including squamous metaplasia and local eosinophilia. Previous studies have shown that mometasone furoate aqueous Nasal spray is effective and well tolerated in reducing perennial rhinitis and seasonal allergic rhinitis symptoms. We undertook a multicenter, open-label study to evaluate, by Nasal Biopsy, the tissue changes associated with mometasone furoate use (200 microg/day) during a 12-month treatment period in patients with perennial rhinitis. Of the 69 patients enrolled in the study, 52 completed all 12 months of treatment. Nasal Biopsy specimens obtained from patients at baseline and after treatment were evaluated in a blinded fashion by computerized image analysis, qualitative histologic examination, and immunocytochemistry. Morphologic examination of Nasal Biopsy specimens showed a decrease in focal metaplasia, no change in epithelial thickness, and no sign of atrophy after treatment with mometasone furoate. Immunocytochemical analyses of Nasal Biopsy specimens obtained before and after treatment revealed a significant decrease in major basic protein-positive eosinophils and tryptase-positive mast cells in the epithelium and lamina propria after treatment. Mometasone furoate appeared to attenuate the inflammatory process by reducing the extent of inflammatory cell infiltration, particularly of eosinophils. This study demonstrated that long-term administration of mometasone furoate is not associated with adverse tissue changes in the Nasal mucosa of patients with perennial rhinitis.
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grass pollen immunotherapy inhibits allergen induced infiltration of cd4 t lymphocytes and eosinophils in the Nasal mucosa and increases the number of cells expressing messenger rna for interferon γ
The Journal of Allergy and Clinical Immunology, 1996Co-Authors: S R Durham, I S Mackay, Sun Ying, Veronica A Varney, M R Jacobson, Robert M Sudderick, Barry A Kay, Qutayba HamidAbstract:Abstract BACKGROUND: Grass pollen injection immunotherapy is effective in patients with summer hay fever, although efficacy must be balanced against possible side effects. The mechanism of immunotherapy is unknown but may be related to its ability to inhibit allergen-induced late responses, which are known to be characterized by infiltration of T lymphocytes, eosinophils, and cells with messenger RNA for so-called T H 2 -type cytokines (IL-4 and IL-5). OBJECTIVE: This study was designed to observe the effect of grass pollen immunotherapy on late Nasal responses and associated cellular infiltration and cytokine mRNA expression. METHODS: We performed local Nasal provocation with grass pollen (and a control challenge) in 28 patients after a 12-month double-blind, placebo-controlled trial of immunotherapy. Nasal Biopsy specimens were obtained at 24 hours and processed for immunohistology and in situ hybridization studies. RESULTS: Grass pollen immunotherapy inhibited allergen-induced immediate (0 to 60 minutes) increases in sneezing ( p p p + T lymphocytes and total (major basic protein–containing) and "activated" (cationic protein–secreting) eosinophils (all p = 0.03). There was a significant ( p = 0.04) increase in cells expressing mRNA for interferon-γ at 24 hours after allergen challenge, which correlated inversely with patients' seasonal symptoms ( r = -0.65, p r = -0.75, p CONCLUSION: The results suggest that successful grass pollen immunotherapy for summer hay fever may act by inhibiting allergen-induced T lymphocyte and eosinophil recruitment and eosinophil activation in the target organ, possibly through a mechanism involving protective local increases in T H 1 -type cells. (J ALLERGY CLIN IMMUNOL 1996;97:1356-65.)
M R Jacobson - One of the best experts on this subject based on the ideXlab platform.
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Objective monitoring of Nasal airway inflammation in rhinitis
The Journal of Allergy and Clinical Immunology, 2005Co-Authors: Peter H. Howarth, M R Jacobson, S R Durham, Carl G. A. Persson, Eli O. Meltzer, Philip E. SilkoffAbstract:Allergic rhinitis is an inflammatory Nasal disorder in which a range of different cells participates. A variety of approaches has been used to monitor Nasal inflammation objectively to investigate disease processes and to evaluate the effect of therapeutic intervention. These approaches include Nasal lavage, Nasal cytology, and Nasal Biopsy, together with the more recently established measurement of Nasal nitric oxide (NO) concentration. Although all provide information about Nasal mucosal inflammation, the extent of information that can be obtained by each approach, the ease of sampling, and the complexity of sample handling differ. Such considerations influence the choice of approach when measurement of Nasal inflammation is to be an objective outcome parameter in a clinical trial. In addition, the choice of approach is also determined by the questions or hypotheses that are to be addressed. Nasal lavage is simple and rapid to perform, is well tolerated, and provides a sample that can provide information about luminal cell recruitment, cell activation, and plasma protein extravasation. Nasal cytology involves sampling and recovering mucosal surface cells. It is also easy to perform and is well tolerated in general, although some find that the procedure causes a transient unpleasant sensation. A differential cell count from the sample provides information about relative cell populations. Both Nasal lavage and Nasal cytology are readily applicable to clinical trials. Nasal cytology sample handling is easier, but Nasal lavage offers the advantage of providing considerably greater information from the sample. Nasal Biopsy is a considerably more invasive procedure and requires expertise not only in tissue sampling but also in Biopsy processing. Therefore, it is applicable only in specialist centers. However, Nasal Biopsy is the only sampling technique that directly informs about tissue cellular events, although these may be implied, in part from the other sampling approaches. Tissue specimens can be used to evaluate both protein and gene expression. Measurement of Nasal NO involves expensive equipment but provides an instantaneous result, unlike the other approaches, all of which require sample processing and analysis. Recommendations for standardization of measurement have been made, and measures are considered in part to reflect allergic inflammation within the Nasal mucosa. The limitations of Nasal NO are that it reflects only a certain aspect of allergic mucosal inflammation, and that because a proportion of Nasally measured NO is derived from the sinuses under normal circumstances, Nasal NO is not specific for Nasal disease. The high contribution from the sinus mucosa limits the discriminatory ability of Nasal NO to reflect Nasal tissue-specific alterations. The incorporation of measures of Nasal inflammation in clinical trials has distinguished anti-inflammatory therapy from symptomatic therapy and has the potential to provide information about the efficacy of novel therapies for allergic rhinitis.
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effect of topical corticosteroids on seasonal increases in epithelial eosinophils and mast cells in allergic rhinitis a comparison of Nasal brush and Biopsy methods
Clinical & Experimental Allergy, 1999Co-Authors: M R Jacobson, S Juliusson, O Lowhagen, B Balder, S R DurhamAbstract:Background Nasal brushing and Nasal Biopsy are well-tolerated sampling techniques. Seasonal grass pollen-induced rhinitis is characterized by epithelial mast cell infiltration and seasonal increases in both epithelial and sub-mucosal eosinophils. Objective To compare the ability of the Nasal brush and Nasal Biopsy techniques to detect natural seasonal increases in eosinophils and mast cells, and to assess the influence of topical corticosteroid. Methods Nasal brush samples and Nasal biopsies were collected from 46 grass pollen-sensitive seasonal rhinitis patients before the grass pollen season and at the peak of the pollen season following 6 weeks' treatment with either fluticasone propionate aqueous Nasal spray (200 μg, twice daily) or placebo Nasal spray. Results Placebo patients showed seasonal increases in epithelial eosinophils both with Nasal brushing (P < 0.0001) and Biopsy (P < 0.001). Epithelial mast cell numbers also increased during the pollen season as detectable by brushing (P < 0.0001) and Biopsy (P < 0.03). Changes in cell numbers measured by Nasal brushing correlated with those observed with Nasal Biopsy, both for eosinophils and mast cells (P < 0.05). Sub-mucosal eosinophils but not mast cells also increased during the pollen season (P < 0.002). Nasal brushing and Biopsy revealed that fluticasone treatment inhibited seasonal increases in epithelial eosinophils (P < 0.00001) and epithelial infiltration by mast cells (Nasal brushing P < 0.00001 and Nasal Biopsy P < 0.01). Fluticasone also inhibited seasonal increases in sub-mucosal eosinophils (P < 0.001) and significantly reduced Nasal symptoms (P < 0.001). Conclusion Nasal brushing harvests sufficient inflammatory cells from the surface of the Nasal mucosa to be used in lieu of Nasal biopsies in observation of the effect of drugs on the Nasal epithelium.
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Effect of topical corticosteroids on seasonal increases in epithelial eosinophils and mast cells in allergic rhinitis: a comparison of Nasal brush and Biopsy methods
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 1999Co-Authors: M R Jacobson, S Juliusson, O Lowhagen, B Balder, A. B. Kay, S R DurhamAbstract:Background Nasal brushing and Nasal Biopsy are well-tolerated sampling techniques. Seasonal grass pollen-induced rhinitis is characterized by epithelial mast cell infiltration and seasonal increases in both epithelial and sub-mucosal eosinophils. Objective To compare the ability of the Nasal brush and Nasal Biopsy techniques to detect natural seasonal increases in eosinophils and mast cells, and to assess the influence of topical corticosteroid. Methods Nasal brush samples and Nasal biopsies were collected from 46 grass pollen-sensitive seasonal rhinitis patients before the grass pollen season and at the peak of the pollen season following 6 weeks' treatment with either fluticasone propionate aqueous Nasal spray (200 μg, twice daily) or placebo Nasal spray. Results Placebo patients showed seasonal increases in epithelial eosinophils both with Nasal brushing (P
R J Davies - One of the best experts on this subject based on the ideXlab platform.
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assessment by Nasal Biopsy of long term use of mometasone furoate aqueous Nasal spray nasonex in the treatment of perennial rhinitis
Otolaryngology-Head and Neck Surgery, 1998Co-Authors: E Minshall, Omar Ghaffar, L Cameron, F Obrien, H Quinn, Julian Rowejones, R J Davies, A Prior, Valerie J Lund, I S MackayAbstract:Abstract Allergic rhinitis is associated with specific histopathologic changes in the Nasal mucosa including squamous metaplasia and local eosinophilia. Previous studies have shown that mometasone furoate aqueous Nasal spray is effective and well tolerated in reducing perennial rhinitis and seasonal allergic rhinitis symptoms. We undertook a multicenter, open-label study to evaluate, by Nasal Biopsy, the tissue changes associated with mometasone furoate use (200 μg/day) during a 12-month treatment period in patients with perennial rhinitis. Of the 69 patients enrolled in the study, 52 completed all 12 months of treatment. Nasal Biopsy specimens obtained from patients at baseline and after treatment were evaluated in a blinded fashion by computerized image analysis, qualitative histologic examination, and immunocytochemistry. Morphologic examination of Nasal Biopsy specimens showed a decrease in focal metaplasia, no change in epithelial thickness, and no sign of atrophy after treatment with mometasone furoate. Immunocytochemical analyses of Nasal Biopsy specimens obtained before and after treatment revealed a significant decrease in major basic protein–positive eosinophils and tryptase-positive mast cells in the epithelium and lamina propria after treatment. Mometasone furoate appeared to attenuate the inflammatory process by reducing the extent of inflammatory cell infiltration, particularly of eosinophils. This study demonstrated that long-term administration of mometasone furoate is not associated with adverse tissue changes in the Nasal mucosa of patients with perennial rhinitis. (Otolaryngol Head Neck Surg 1998;118:648-54.)
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Assessment by Nasal Biopsy of long-term use of mometasone furoate aqueous Nasal spray (Nasonex) in the treatment of perennial rhinitis.
Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 1998Co-Authors: E Minshall, Omar Ghaffar, L Cameron, H Quinn, R J Davies, A Prior, Valerie J Lund, F. O'brien, Julian Rowe-jones, I S MackayAbstract:Allergic rhinitis is associated with specific histopathologic changes in the Nasal mucosa including squamous metaplasia and local eosinophilia. Previous studies have shown that mometasone furoate aqueous Nasal spray is effective and well tolerated in reducing perennial rhinitis and seasonal allergic rhinitis symptoms. We undertook a multicenter, open-label study to evaluate, by Nasal Biopsy, the tissue changes associated with mometasone furoate use (200 microg/day) during a 12-month treatment period in patients with perennial rhinitis. Of the 69 patients enrolled in the study, 52 completed all 12 months of treatment. Nasal Biopsy specimens obtained from patients at baseline and after treatment were evaluated in a blinded fashion by computerized image analysis, qualitative histologic examination, and immunocytochemistry. Morphologic examination of Nasal Biopsy specimens showed a decrease in focal metaplasia, no change in epithelial thickness, and no sign of atrophy after treatment with mometasone furoate. Immunocytochemical analyses of Nasal Biopsy specimens obtained before and after treatment revealed a significant decrease in major basic protein-positive eosinophils and tryptase-positive mast cells in the epithelium and lamina propria after treatment. Mometasone furoate appeared to attenuate the inflammatory process by reducing the extent of inflammatory cell infiltration, particularly of eosinophils. This study demonstrated that long-term administration of mometasone furoate is not associated with adverse tissue changes in the Nasal mucosa of patients with perennial rhinitis.
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A comparison of cytokine release from epithelial cells cultured from Nasal Biopsy specimens of atopic patients with and without rhinitis and nonatopic subjects without rhinitis.
The Journal of allergy and clinical immunology, 1997Co-Authors: Moises A. Calderon, Jagdish L. Devalia, Andrew J. Prior, Rj Sapsford, R J DaviesAbstract:Recent studies have suggested that airway epithelial cells of atopic and nonatopic individuals may differ in their ability to produce proinflammatory cytokines. We have cultured human Nasal epithelial cells (NECs) as confluent explant cultures from Nasal Biopsy specimens of well-characterized nonatopic normal volunteers without rhinitis (n = 8), atopic volunteers without rhinitis (n = 9), and atopic patient volunteers with rhinitis (n = 10) and measured the amounts of IL-1 beta, IL-8, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor-alpha, and RANTES released spontaneously into the culture medium by these cells in vitro. NECs from patients with allergic rhinitis were cultured from Biopsy specimens obtained on two different occasions, during and after the pollen season. In general, NECs from atopic individuals released significantly greater amounts of IL-1 beta, IL-8, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor-alpha, and RANTES than NECs from nonatopic individuals. IL-8 was released in greatest quantity and IL-1 beta in lowest quantity, regardless of whether the NECs were derived from atopic or nonatopic volunteers. Of the atopic individuals, NECs of atopic patients with rhinitis naturally exposed to pollen released greater quantities of all these cytokines, compared with NECs of atopic patients with rhinitis and atopic patients without rhinitis who were not exposed to allergen. These results suggest that NECs of atopic individuals, who are genetically predisposed to upper airway disease, release increased amounts of proinflammatory cytokines and that natural exposure to allergen enhances the release of these cytokines, exacerbating the symptoms of allergic disease.
Carlo Colantuoni - One of the best experts on this subject based on the ideXlab platform.
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Olfactory cells via Nasal Biopsy reflect the developing brain in gene expression profiles: utility and limitation of the surrogate tissues in research for brain disorders.
Neuroscience research, 2013Co-Authors: Yasue Horiuchi, Koko Ishizuka, Nicola G. Cascella, Shin Ichi Kano, Seiji Ishii, C. Conover Talbot, Andrew E. Jaffe, Hideyuki Okano, Jonathan Pevsner, Carlo ColantuoniAbstract:Human olfactory cells obtained by rapid Nasal Biopsy have been suggested to be a good surrogate system to address brain disease-associated molecular changes. Nonetheless, whether use of this experimental strategy is justified remains unclear. Here we compared expression profiles of olfactory cells systematically with those from the brain tissues and other cells. Principal component analysis indicated that the expression profiles of olfactory cells are very different from those of blood cells, but are closer to those of stem cells, in particular mesenchymal stem cells, that can be differentiated into the cells of the central nervous system.
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Neuronal biomarkers from patients with mental illnesses: a novel method through Nasal Biopsy combined with laser-captured microdissection.
Molecular psychiatry, 2010Co-Authors: Katsunori Tajinda, Koko Ishizuka, Carlo Colantuoni, M Morita, Jessica M. Winicki, Sandra Y. Lin, David J. Schretlen, Akira Sawa, Nicola G. CascellaAbstract:Neuronal biomarkers from patients with mental illnesses: a novel method through Nasal Biopsy combined with laser-captured microdissection
