The Experts below are selected from a list of 102 Experts worldwide ranked by ideXlab platform

Gholam Hossein Kazemzadeh - One of the best experts on this subject based on the ideXlab platform.

  • fabrication and characterization of novel compliant small diameter pet pu pcl triad hybrid vascular graft
    Biomedical Materials, 2020
    Co-Authors: Nafiseh Jirofti, Davod Mohebbikalhori, Abdolreza Samimi, Afra Hadjizadeh, Gholam Hossein Kazemzadeh
    Abstract:

    Nanomaterial structures are highly contributive in TEVS due to their ability to mimic the nano-scale dimension of the natural extra-cellular matrix (ECM) and the existing mechanical match between the Native Blood Vessel and the scaffold as a vascular graft. The aim of this study was developing and mechanically improving of the nano-fibrous triad-hybrid scaffolds with different composite ratios of the polyethylene terephthalate (PET), polyurethane (PU) and polycaprolactone (PCL). The morphological, biological, mechanical and, biomechanical properties of the neat and hybrid structures were examined using SEM, DSC, FTIR, Tensile, Compliance, Burst pressure, MTT assay and, implanted the specimens under rat skin to explore the immune system in vivo. Results showed that the fiber diameter and porosity changes in the triad-hybrid electrospun scaffold ranged within 388 +/- 88 to 547 +/- 89 nm and 56.60 +/- 2.06 % to 75.00 +/- 1.94 %, respectively. In addition, the change's range of the tensile and force in the scaffolds were within 2.7 +/- 0.44 to 5.27+/- 0.83 MPa and 2.68 +/-0.19 to 10.03+/- 0.75 MPa, respectively. Also, the compliance and burst pressure of the structures were reported 4.05 +/- 0.21 to 7.09 +/- 0.49 and 1623 +/- 329 to 2560 +/- 121 mmHg, respectively. According to the MTT assay, high cell viability was observed on the triad-hybrid structures with a high percentage of PET when compared to that of PU. Findings of this research demonstrate that PET/PU/PCL triad-hybrid vascular scaffold has enough potential to be used in vascular tissue engineering application.

  • Fabrication and characterization of novel compliant small-diameter PET/PU/PCL triad-hybrid vascular graft.
    Biomedical Materials, 2020
    Co-Authors: Nafiseh Jirofti, Abdolreza Samimi, Afra Hadjizadeh, Davod Mohebbi-kalhori, Gholam Hossein Kazemzadeh
    Abstract:

    Nanomaterial structures are highly contributive in TEVS due to their ability to mimic the nano-scale dimension of the natural extra-cellular matrix (ECM) and the existing mechanical match between the Native Blood Vessel and the scaffold as a vascular graft. The aim of this study was developing and mechanically improving of the nano-fibrous triad-hybrid scaffolds with different composite ratios of the polyethylene terephthalate (PET), polyurethane (PU) and polycaprolactone (PCL). The morphological, biological, mechanical and, biomechanical properties of the neat and hybrid structures were examined using SEM, DSC, FTIR, Tensile, Compliance, Burst pressure, MTT assay and, implanted the specimens under rat skin to explore the immune system in vivo. Results showed that the fiber diameter and porosity changes in the triad-hybrid electrospun scaffold ranged within 388 +/- 88 to 547 +/- 89 nm and 56.60 +/- 2.06 % to 75.00 +/- 1.94 %, respectively. In addition, the change's range of the tensile and force in the scaffolds were within 2.7 +/- 0.44 to 5.27+/- 0.83 MPa and 2.68 +/-0.19 to 10.03+/- 0.75 MPa, respectively. Also, the compliance and burst pressure of the structures were reported 4.05 +/- 0.21 to 7.09 +/- 0.49 and 1623 +/- 329 to 2560 +/- 121 mmHg, respectively. According to the MTT assay, high cell viability was observed on the triad-hybrid structures with a high percentage of PET when compared to that of PU. Findings of this research demonstrate that PET/PU/PCL triad-hybrid vascular scaffold has enough potential to be used in vascular tissue engineering application.

Nafiseh Jirofti - One of the best experts on this subject based on the ideXlab platform.

  • fabrication and characterization of novel compliant small diameter pet pu pcl triad hybrid vascular graft
    Biomedical Materials, 2020
    Co-Authors: Nafiseh Jirofti, Davod Mohebbikalhori, Abdolreza Samimi, Afra Hadjizadeh, Gholam Hossein Kazemzadeh
    Abstract:

    Nanomaterial structures are highly contributive in TEVS due to their ability to mimic the nano-scale dimension of the natural extra-cellular matrix (ECM) and the existing mechanical match between the Native Blood Vessel and the scaffold as a vascular graft. The aim of this study was developing and mechanically improving of the nano-fibrous triad-hybrid scaffolds with different composite ratios of the polyethylene terephthalate (PET), polyurethane (PU) and polycaprolactone (PCL). The morphological, biological, mechanical and, biomechanical properties of the neat and hybrid structures were examined using SEM, DSC, FTIR, Tensile, Compliance, Burst pressure, MTT assay and, implanted the specimens under rat skin to explore the immune system in vivo. Results showed that the fiber diameter and porosity changes in the triad-hybrid electrospun scaffold ranged within 388 +/- 88 to 547 +/- 89 nm and 56.60 +/- 2.06 % to 75.00 +/- 1.94 %, respectively. In addition, the change's range of the tensile and force in the scaffolds were within 2.7 +/- 0.44 to 5.27+/- 0.83 MPa and 2.68 +/-0.19 to 10.03+/- 0.75 MPa, respectively. Also, the compliance and burst pressure of the structures were reported 4.05 +/- 0.21 to 7.09 +/- 0.49 and 1623 +/- 329 to 2560 +/- 121 mmHg, respectively. According to the MTT assay, high cell viability was observed on the triad-hybrid structures with a high percentage of PET when compared to that of PU. Findings of this research demonstrate that PET/PU/PCL triad-hybrid vascular scaffold has enough potential to be used in vascular tissue engineering application.

  • Fabrication and characterization of novel compliant small-diameter PET/PU/PCL triad-hybrid vascular graft.
    Biomedical Materials, 2020
    Co-Authors: Nafiseh Jirofti, Abdolreza Samimi, Afra Hadjizadeh, Davod Mohebbi-kalhori, Gholam Hossein Kazemzadeh
    Abstract:

    Nanomaterial structures are highly contributive in TEVS due to their ability to mimic the nano-scale dimension of the natural extra-cellular matrix (ECM) and the existing mechanical match between the Native Blood Vessel and the scaffold as a vascular graft. The aim of this study was developing and mechanically improving of the nano-fibrous triad-hybrid scaffolds with different composite ratios of the polyethylene terephthalate (PET), polyurethane (PU) and polycaprolactone (PCL). The morphological, biological, mechanical and, biomechanical properties of the neat and hybrid structures were examined using SEM, DSC, FTIR, Tensile, Compliance, Burst pressure, MTT assay and, implanted the specimens under rat skin to explore the immune system in vivo. Results showed that the fiber diameter and porosity changes in the triad-hybrid electrospun scaffold ranged within 388 +/- 88 to 547 +/- 89 nm and 56.60 +/- 2.06 % to 75.00 +/- 1.94 %, respectively. In addition, the change's range of the tensile and force in the scaffolds were within 2.7 +/- 0.44 to 5.27+/- 0.83 MPa and 2.68 +/-0.19 to 10.03+/- 0.75 MPa, respectively. Also, the compliance and burst pressure of the structures were reported 4.05 +/- 0.21 to 7.09 +/- 0.49 and 1623 +/- 329 to 2560 +/- 121 mmHg, respectively. According to the MTT assay, high cell viability was observed on the triad-hybrid structures with a high percentage of PET when compared to that of PU. Findings of this research demonstrate that PET/PU/PCL triad-hybrid vascular scaffold has enough potential to be used in vascular tissue engineering application.

Afra Hadjizadeh - One of the best experts on this subject based on the ideXlab platform.

  • fabrication and characterization of novel compliant small diameter pet pu pcl triad hybrid vascular graft
    Biomedical Materials, 2020
    Co-Authors: Nafiseh Jirofti, Davod Mohebbikalhori, Abdolreza Samimi, Afra Hadjizadeh, Gholam Hossein Kazemzadeh
    Abstract:

    Nanomaterial structures are highly contributive in TEVS due to their ability to mimic the nano-scale dimension of the natural extra-cellular matrix (ECM) and the existing mechanical match between the Native Blood Vessel and the scaffold as a vascular graft. The aim of this study was developing and mechanically improving of the nano-fibrous triad-hybrid scaffolds with different composite ratios of the polyethylene terephthalate (PET), polyurethane (PU) and polycaprolactone (PCL). The morphological, biological, mechanical and, biomechanical properties of the neat and hybrid structures were examined using SEM, DSC, FTIR, Tensile, Compliance, Burst pressure, MTT assay and, implanted the specimens under rat skin to explore the immune system in vivo. Results showed that the fiber diameter and porosity changes in the triad-hybrid electrospun scaffold ranged within 388 +/- 88 to 547 +/- 89 nm and 56.60 +/- 2.06 % to 75.00 +/- 1.94 %, respectively. In addition, the change's range of the tensile and force in the scaffolds were within 2.7 +/- 0.44 to 5.27+/- 0.83 MPa and 2.68 +/-0.19 to 10.03+/- 0.75 MPa, respectively. Also, the compliance and burst pressure of the structures were reported 4.05 +/- 0.21 to 7.09 +/- 0.49 and 1623 +/- 329 to 2560 +/- 121 mmHg, respectively. According to the MTT assay, high cell viability was observed on the triad-hybrid structures with a high percentage of PET when compared to that of PU. Findings of this research demonstrate that PET/PU/PCL triad-hybrid vascular scaffold has enough potential to be used in vascular tissue engineering application.

  • Fabrication and characterization of novel compliant small-diameter PET/PU/PCL triad-hybrid vascular graft.
    Biomedical Materials, 2020
    Co-Authors: Nafiseh Jirofti, Abdolreza Samimi, Afra Hadjizadeh, Davod Mohebbi-kalhori, Gholam Hossein Kazemzadeh
    Abstract:

    Nanomaterial structures are highly contributive in TEVS due to their ability to mimic the nano-scale dimension of the natural extra-cellular matrix (ECM) and the existing mechanical match between the Native Blood Vessel and the scaffold as a vascular graft. The aim of this study was developing and mechanically improving of the nano-fibrous triad-hybrid scaffolds with different composite ratios of the polyethylene terephthalate (PET), polyurethane (PU) and polycaprolactone (PCL). The morphological, biological, mechanical and, biomechanical properties of the neat and hybrid structures were examined using SEM, DSC, FTIR, Tensile, Compliance, Burst pressure, MTT assay and, implanted the specimens under rat skin to explore the immune system in vivo. Results showed that the fiber diameter and porosity changes in the triad-hybrid electrospun scaffold ranged within 388 +/- 88 to 547 +/- 89 nm and 56.60 +/- 2.06 % to 75.00 +/- 1.94 %, respectively. In addition, the change's range of the tensile and force in the scaffolds were within 2.7 +/- 0.44 to 5.27+/- 0.83 MPa and 2.68 +/-0.19 to 10.03+/- 0.75 MPa, respectively. Also, the compliance and burst pressure of the structures were reported 4.05 +/- 0.21 to 7.09 +/- 0.49 and 1623 +/- 329 to 2560 +/- 121 mmHg, respectively. According to the MTT assay, high cell viability was observed on the triad-hybrid structures with a high percentage of PET when compared to that of PU. Findings of this research demonstrate that PET/PU/PCL triad-hybrid vascular scaffold has enough potential to be used in vascular tissue engineering application.

Abdolreza Samimi - One of the best experts on this subject based on the ideXlab platform.

  • fabrication and characterization of novel compliant small diameter pet pu pcl triad hybrid vascular graft
    Biomedical Materials, 2020
    Co-Authors: Nafiseh Jirofti, Davod Mohebbikalhori, Abdolreza Samimi, Afra Hadjizadeh, Gholam Hossein Kazemzadeh
    Abstract:

    Nanomaterial structures are highly contributive in TEVS due to their ability to mimic the nano-scale dimension of the natural extra-cellular matrix (ECM) and the existing mechanical match between the Native Blood Vessel and the scaffold as a vascular graft. The aim of this study was developing and mechanically improving of the nano-fibrous triad-hybrid scaffolds with different composite ratios of the polyethylene terephthalate (PET), polyurethane (PU) and polycaprolactone (PCL). The morphological, biological, mechanical and, biomechanical properties of the neat and hybrid structures were examined using SEM, DSC, FTIR, Tensile, Compliance, Burst pressure, MTT assay and, implanted the specimens under rat skin to explore the immune system in vivo. Results showed that the fiber diameter and porosity changes in the triad-hybrid electrospun scaffold ranged within 388 +/- 88 to 547 +/- 89 nm and 56.60 +/- 2.06 % to 75.00 +/- 1.94 %, respectively. In addition, the change's range of the tensile and force in the scaffolds were within 2.7 +/- 0.44 to 5.27+/- 0.83 MPa and 2.68 +/-0.19 to 10.03+/- 0.75 MPa, respectively. Also, the compliance and burst pressure of the structures were reported 4.05 +/- 0.21 to 7.09 +/- 0.49 and 1623 +/- 329 to 2560 +/- 121 mmHg, respectively. According to the MTT assay, high cell viability was observed on the triad-hybrid structures with a high percentage of PET when compared to that of PU. Findings of this research demonstrate that PET/PU/PCL triad-hybrid vascular scaffold has enough potential to be used in vascular tissue engineering application.

  • Fabrication and characterization of novel compliant small-diameter PET/PU/PCL triad-hybrid vascular graft.
    Biomedical Materials, 2020
    Co-Authors: Nafiseh Jirofti, Abdolreza Samimi, Afra Hadjizadeh, Davod Mohebbi-kalhori, Gholam Hossein Kazemzadeh
    Abstract:

    Nanomaterial structures are highly contributive in TEVS due to their ability to mimic the nano-scale dimension of the natural extra-cellular matrix (ECM) and the existing mechanical match between the Native Blood Vessel and the scaffold as a vascular graft. The aim of this study was developing and mechanically improving of the nano-fibrous triad-hybrid scaffolds with different composite ratios of the polyethylene terephthalate (PET), polyurethane (PU) and polycaprolactone (PCL). The morphological, biological, mechanical and, biomechanical properties of the neat and hybrid structures were examined using SEM, DSC, FTIR, Tensile, Compliance, Burst pressure, MTT assay and, implanted the specimens under rat skin to explore the immune system in vivo. Results showed that the fiber diameter and porosity changes in the triad-hybrid electrospun scaffold ranged within 388 +/- 88 to 547 +/- 89 nm and 56.60 +/- 2.06 % to 75.00 +/- 1.94 %, respectively. In addition, the change's range of the tensile and force in the scaffolds were within 2.7 +/- 0.44 to 5.27+/- 0.83 MPa and 2.68 +/-0.19 to 10.03+/- 0.75 MPa, respectively. Also, the compliance and burst pressure of the structures were reported 4.05 +/- 0.21 to 7.09 +/- 0.49 and 1623 +/- 329 to 2560 +/- 121 mmHg, respectively. According to the MTT assay, high cell viability was observed on the triad-hybrid structures with a high percentage of PET when compared to that of PU. Findings of this research demonstrate that PET/PU/PCL triad-hybrid vascular scaffold has enough potential to be used in vascular tissue engineering application.

T H Van Kuppevelt - One of the best experts on this subject based on the ideXlab platform.

  • Vascular replacement using a layered elastin-collagen vascular graft in a porcine model: one week patency versus one month occlusion
    Organogenesis, 2015
    Co-Authors: Martin Koens, J A Van Der Vliet, A G Krasznai, Willeke F Daamen, A. Hanssen, Thijs Hendriks, R Praster, T H Van Kuppevelt
    Abstract:

    abstractA persistent clinical demand exists for a suitable arterial prosthesis. In this study, a vascular conduit mimicking the Native 3-layered artery, and constructed from the extracellular matrix proteins type I collagen and elastin, was evaluated for its performance as a Blood Vessel equivalent.A tubular 3-layered graft (elastin-collagen-collagen) was prepared using highly purified type I collagen fibrils and elastin fibers, resembling the 3-layered Native Blood Vessel architecture. The vascular graft was crosslinked and heparinised (37 ± 4 μg heparin/mg graft), and evaluated as a vascular graft using a porcine bilateral iliac artery model. An intra-animal comparison with clinically-used heparinised ePTFE (Propaten®) was made. Analyses included biochemical characterization, duplex scanning, (immuno)histochemistry and scanning electron microscopy.The tubular graft was easy to handle with adequate suturability. Implantation resulted in pulsating grafts without leakage. One week after implantation, both ...

  • controlled fabrication of triple layered and molecularly defined collagen elastin vascular grafts resembling the Native Blood Vessel
    Acta Biomaterialia, 2010
    Co-Authors: Martin J W Koens, Kaeuis A Faraj, Ronnie G Wismans, J A Van Der Vliet, A G Krasznai, Vincent M J I Cuijpers, John A Jansen, Willeke F Daamen, T H Van Kuppevelt
    Abstract:

    Abstract There is a consistent need for a suitable natural biomaterial to function as an arterial prosthesis in achieving arterial regeneration. Natural grafts are generally obtained by decellularization of Native Blood Vessels, but batch to batch variations may occur and the nature/content of remaining contaminants is generally unknown. In this study we fabricated a molecularly defined natural arterial graft from scratch resembling the Native three layered architecture from the fibrillar extracellular matrix components collagen and elastin. Using casting, moulding, freezing and lyophilization techniques, a triple layered construct was prepared consisting of an inner layer of elastin fibres, a middle (porous) film layer of collagen fibrils and an outer scaffold layer of collagen fibrils. The construct was carbodiimide cross-linked and heparinized. Characterization included biochemical/biophysical analyses, scanning electron microscopy, micro-computed tomography, (immuno)histology and haemocompatibility. Burst pressures were up to 400 mm Hg and largely conferred by the intermediate porous collagen film layer. The highly purified type I collagen fibrils and elastin fibres used did not evoke platelet aggregation in vitro. Suturability of the graft in end to side anastomosis was successful and considered adequate for in vivo application.

  • Controlled fabrication of triple layered and molecularly defined collagen/elastin vascular grafts resembling the Native Blood Vessel.
    Acta Biomaterialia, 2010
    Co-Authors: Martin J W Koens, Kaeuis A Faraj, Ronnie G Wismans, J A Van Der Vliet, A G Krasznai, Vincent M J I Cuijpers, John A Jansen, Willeke F Daamen, T H Van Kuppevelt
    Abstract:

    There is a consistent need for a suitable natural biomaterial to function as an arterial prosthesis in achieving arterial regeneration. Natural grafts are generally obtained by decellularization of Native Blood Vessels, but batch to batch variations may occur and the nature/content of remaining contaminants is generally unknown. In this study we fabricated a molecularly defined natural arterial graft from scratch resembling the Native three layered architecture from the fibrillar extracellular matrix components collagen and elastin. Using casting, moulding, freezing and lyophilization techniques, a triple layered construct was prepared consisting of an inner layer of elastin fibres, a middle (porous) film layer of collagen fibrils and an outer scaffold layer of collagen fibrils. The construct was carbodiimide cross-linked and heparinized. Characterization included biochemical/biophysical analyses, scanning electron microscopy, micro-computed tomography, (immuno)histology and haemocompatibility. Burst pressures were up to 400mm Hg and largely conferred by the intermediate porous collagen film layer. The highly purified type I collagen fibrils and elastin fibres used did not evoke platelet aggregation in vitro. Suturability of the graft in end to side anastomosis was successful and considered adequate for in vivo application.