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Joaquim Coutinhonetto - One of the best experts on this subject based on the ideXlab platform.
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growth factors expression and ultrastructural morphology after application of low level laser and Natural Latex protein on a sciatic nerve crush type injury
PLOS ONE, 2019Co-Authors: Fernando José Dias, Joaquim Coutinhonetto, Valéria Paula Sassoli Fazan, Diego Pulzatto Cury, Sonia Regina Yokomizo De Almeida, Eduardo Borie, Ramon Fuentes, Iisei WatanabeAbstract:The effects of low-level laser therapy (LLLT) and Natural Latex protein (F1, Hevea brasiliensis) were evaluated on crush-type injuries (15kg) to the sciatic nerve in the expressions of nerve growth factor (NGF) and vascular endothelium growth factor (VEGF) and ultrastructural morphology to associate with previous morphometric data using the same protocol of injury and treatment. Thirty-six male rats were allocated into six experimental groups (n = 6): 1-Control; 2-Exposed nerve; 3-Injured nerve; 4-LLLT (15J/cm2, 780nm, 30mW, Continuous Wave) treated injured nerve; 5-F1 (0,1mg) treated injured nerve; and 6-LLLTF Q1:0; Q3:55.5%area). Among them, the Injured (Med:70.7; Q1:64.4; Q3:77.5%area) showed the highest expression, and F1 (Med:17.3; Q1:14.1; Q3:21.7%area) had the lowest. At week 8, NGF expressions decreased in the injured groups. VEGF was expressed in all groups; its higher expression was observed in the injured groups 4 weeks after (Injured. Med:29.5; F1. Med:17.7 and LLLTF Q1.30.6; Q3.39.6%area) and LLLTF Q1:16; Q3:25%area). Ultrastructural morphology revealed improvements in the treated groups; 4 weeks after, the F1 group presented greater quantity and diameter of the nerve fibers uniformly distributed. Eight weeks after, the F1 and LLLTF at 8th week F1 and LLLT&F1 groups presented better features and higher vascularization that could be associated with VEGF maintenance.
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properties of the tibialis anterior muscle after treatment with laser therapy and Natural Latex protein following sciatic nerve crush
Muscle & Nerve, 2015Co-Authors: Kenia Lemos Muniz, Joaquim Coutinhonetto, Luiz Gustavo De Sousa, Thais Tedeschi Dos Santos, Vanessa De Oliveira Teles, Ricardo Alexandre Junqueira Calzzani, Mamie Mizusaki Iyomasa, Iisei Watanabe, Fernando José Dias, Valéria Paula Sassoli FazanAbstract:Introduction: In this study we evaluated the characteristics of the tibialis anterior muscle after sciatic nerve crush and treatment with low-level laser therapy (LLLT) or the protein from Natural Latex (P1). Methods: We studied the following 6 groups of male Wistar rats: control (CG); exposed nerve (EG); injured nerve (IG); injured nerve with LLLT (LG); injured nerve with P1 (PG); and injured nerve with P1 and LLLT (LPG). Results: After 4 weeks, muscle morphology showed improvement in the treated groups; after 8 weeks, the treated groups resembled controls, especially the PG. Morphometry revealed muscle fiber atrophy after nerve injury, with time-dependent recovery. Histochemical analysis revealed increased intermediate fiber area. The PG was more similar to controls with NADH staining, whereas the LPG more closely resembled controls with SDH staining. Conclusion: Treatment using only P1 proved most efficient, revealing a negative interaction between P1 and LLLT. Muscle Nerve 52: 869–875, 2015
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increased vascular permeability angiogenesis and wound healing induced by the serum of Natural Latex of the rubber tree hevea brasiliensis
Phytotherapy Research, 2010Co-Authors: Ricardo Mendonca, Vanessa Beatriz Mauricio, Larissa B Teixeira, Joao Jose Lachat, Joaquim CoutinhonettoAbstract:Increases in vascular permeability and angiogenesis are crucial events to wound repair, tumoral growth and revascularization of tissues submitted to ischemia. An increased vascular permeability allows a variety of cytokines and growth factors to reach the damaged tissue. Nevertheless, the angiogenesis supply tissues with a wide variety of nutrients and is also important to metabolites clearance. It has been suggested that the Natural Latex from Hevea brasiliensis showed wound healing properties and angiogenic activity. Thus, the purpose of this work was to characterize its angiogenic activity and its effects on vascular permeability and wound healing. The serum fraction of the Latex was separated from the rubber with reduction of the pH. The activity of the dialyzed serum fraction on the vascular permeability injected in subcutaneous tissue was assayed according Mile's method. The angiogenic activity was determined using a chick embryo chorioallantoic membrane assay and its effects on the wound-healing process was determined by the rabbit ear dermal ulcer model. The serum fraction showed evident angiogenic effect and it was effective in enhancing vascular permeability. In dermal ulcers, this material significantly accelerated wound healing. Moreover, the serum fraction boiled and treated with proteases lost these activities. These results are in accordance with the enhancement of wound healing observed in clinical trials carried out with a biomembrane prepared with the same Natural Latex.
Serge Kochman - One of the best experts on this subject based on the ideXlab platform.
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allergy to Latex avocado pear and banana evidence for a 30 kd antigen in immunoblotting
The Journal of Allergy and Clinical Immunology, 1995Co-Authors: F Lavaud, A Prevost, Claude Cossart, L Guerin, J Bernard, Serge KochmanAbstract:Abstract Allergens of Natural Latex, Latex gloves, avocado pear, and banana extracts were investigated by an immunoblotting technique in sera of patients experiencing associated Latex and fruit allergies. Extracts were separated by sodium dodecyl-sulfate–polyacrylamide gel electrophoresis and electroblotted onto nitrocellulose. After incubation with patients' sera, IgE antibodies were revealed by a goat anti-human IgE alkaline-phosphatase conjugate. Seventeen serum samples from patients with well-documented Latex allergy were studied. Among these patients, 10 demonstrated an allergy to avocado pear sometimes associated with banana. In sera from patients with Latex and fruit allergy, prominent IgE binding was revealed at about 30 kd with Latex and fruit extracts. Serum controls remained negative. Cross-inhibition of immunoblotting confirmed that this main allergen is linked to a common epitope present in Latex and fruits. This must be related to clinical findings and previous observations of cross-reactivity. (J ALLERGY CLIN IMMUNOL1995;95:557-64)
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allergy to Latex avocado pear and banana evidence for a 30 kd antigen in immunoblotting
The Journal of Allergy and Clinical Immunology, 1995Co-Authors: F Lavaud, A Prevost, Claude Cossart, L Guerin, J Bernard, Serge KochmanAbstract:Abstract Allergens of Natural Latex, Latex gloves, avocado pear, and banana extracts were investigated by an immunoblotting technique in sera of patients experiencing associated Latex and fruit allergies. Extracts were separated by sodium dodecyl-sulfate–polyacrylamide gel electrophoresis and electroblotted onto nitrocellulose. After incubation with patients' sera, IgE antibodies were revealed by a goat anti-human IgE alkaline-phosphatase conjugate. Seventeen serum samples from patients with well-documented Latex allergy were studied. Among these patients, 10 demonstrated an allergy to avocado pear sometimes associated with banana. In sera from patients with Latex and fruit allergy, prominent IgE binding was revealed at about 30 kd with Latex and fruit extracts. Serum controls remained negative. Cross-inhibition of immunoblotting confirmed that this main allergen is linked to a common epitope present in Latex and fruits. This must be related to clinical findings and previous observations of cross-reactivity. (J ALLERGY CLIN IMMUNOL1995;95:557-64)
Valéria Paula Sassoli Fazan - One of the best experts on this subject based on the ideXlab platform.
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growth factors expression and ultrastructural morphology after application of low level laser and Natural Latex protein on a sciatic nerve crush type injury
PLOS ONE, 2019Co-Authors: Fernando José Dias, Joaquim Coutinhonetto, Valéria Paula Sassoli Fazan, Diego Pulzatto Cury, Sonia Regina Yokomizo De Almeida, Eduardo Borie, Ramon Fuentes, Iisei WatanabeAbstract:The effects of low-level laser therapy (LLLT) and Natural Latex protein (F1, Hevea brasiliensis) were evaluated on crush-type injuries (15kg) to the sciatic nerve in the expressions of nerve growth factor (NGF) and vascular endothelium growth factor (VEGF) and ultrastructural morphology to associate with previous morphometric data using the same protocol of injury and treatment. Thirty-six male rats were allocated into six experimental groups (n = 6): 1-Control; 2-Exposed nerve; 3-Injured nerve; 4-LLLT (15J/cm2, 780nm, 30mW, Continuous Wave) treated injured nerve; 5-F1 (0,1mg) treated injured nerve; and 6-LLLTF Q1:0; Q3:55.5%area). Among them, the Injured (Med:70.7; Q1:64.4; Q3:77.5%area) showed the highest expression, and F1 (Med:17.3; Q1:14.1; Q3:21.7%area) had the lowest. At week 8, NGF expressions decreased in the injured groups. VEGF was expressed in all groups; its higher expression was observed in the injured groups 4 weeks after (Injured. Med:29.5; F1. Med:17.7 and LLLTF Q1.30.6; Q3.39.6%area) and LLLTF Q1:16; Q3:25%area). Ultrastructural morphology revealed improvements in the treated groups; 4 weeks after, the F1 group presented greater quantity and diameter of the nerve fibers uniformly distributed. Eight weeks after, the F1 and LLLTF at 8th week F1 and LLLT&F1 groups presented better features and higher vascularization that could be associated with VEGF maintenance.
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properties of the tibialis anterior muscle after treatment with laser therapy and Natural Latex protein following sciatic nerve crush
Muscle & Nerve, 2015Co-Authors: Kenia Lemos Muniz, Joaquim Coutinhonetto, Luiz Gustavo De Sousa, Thais Tedeschi Dos Santos, Vanessa De Oliveira Teles, Ricardo Alexandre Junqueira Calzzani, Mamie Mizusaki Iyomasa, Iisei Watanabe, Fernando José Dias, Valéria Paula Sassoli FazanAbstract:Introduction: In this study we evaluated the characteristics of the tibialis anterior muscle after sciatic nerve crush and treatment with low-level laser therapy (LLLT) or the protein from Natural Latex (P1). Methods: We studied the following 6 groups of male Wistar rats: control (CG); exposed nerve (EG); injured nerve (IG); injured nerve with LLLT (LG); injured nerve with P1 (PG); and injured nerve with P1 and LLLT (LPG). Results: After 4 weeks, muscle morphology showed improvement in the treated groups; after 8 weeks, the treated groups resembled controls, especially the PG. Morphometry revealed muscle fiber atrophy after nerve injury, with time-dependent recovery. Histochemical analysis revealed increased intermediate fiber area. The PG was more similar to controls with NADH staining, whereas the LPG more closely resembled controls with SDH staining. Conclusion: Treatment using only P1 proved most efficient, revealing a negative interaction between P1 and LLLT. Muscle Nerve 52: 869–875, 2015
Iisei Watanabe - One of the best experts on this subject based on the ideXlab platform.
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growth factors expression and ultrastructural morphology after application of low level laser and Natural Latex protein on a sciatic nerve crush type injury
PLOS ONE, 2019Co-Authors: Fernando José Dias, Joaquim Coutinhonetto, Valéria Paula Sassoli Fazan, Diego Pulzatto Cury, Sonia Regina Yokomizo De Almeida, Eduardo Borie, Ramon Fuentes, Iisei WatanabeAbstract:The effects of low-level laser therapy (LLLT) and Natural Latex protein (F1, Hevea brasiliensis) were evaluated on crush-type injuries (15kg) to the sciatic nerve in the expressions of nerve growth factor (NGF) and vascular endothelium growth factor (VEGF) and ultrastructural morphology to associate with previous morphometric data using the same protocol of injury and treatment. Thirty-six male rats were allocated into six experimental groups (n = 6): 1-Control; 2-Exposed nerve; 3-Injured nerve; 4-LLLT (15J/cm2, 780nm, 30mW, Continuous Wave) treated injured nerve; 5-F1 (0,1mg) treated injured nerve; and 6-LLLTF Q1:0; Q3:55.5%area). Among them, the Injured (Med:70.7; Q1:64.4; Q3:77.5%area) showed the highest expression, and F1 (Med:17.3; Q1:14.1; Q3:21.7%area) had the lowest. At week 8, NGF expressions decreased in the injured groups. VEGF was expressed in all groups; its higher expression was observed in the injured groups 4 weeks after (Injured. Med:29.5; F1. Med:17.7 and LLLTF Q1.30.6; Q3.39.6%area) and LLLTF Q1:16; Q3:25%area). Ultrastructural morphology revealed improvements in the treated groups; 4 weeks after, the F1 group presented greater quantity and diameter of the nerve fibers uniformly distributed. Eight weeks after, the F1 and LLLTF at 8th week F1 and LLLT&F1 groups presented better features and higher vascularization that could be associated with VEGF maintenance.
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properties of the tibialis anterior muscle after treatment with laser therapy and Natural Latex protein following sciatic nerve crush
Muscle & Nerve, 2015Co-Authors: Kenia Lemos Muniz, Joaquim Coutinhonetto, Luiz Gustavo De Sousa, Thais Tedeschi Dos Santos, Vanessa De Oliveira Teles, Ricardo Alexandre Junqueira Calzzani, Mamie Mizusaki Iyomasa, Iisei Watanabe, Fernando José Dias, Valéria Paula Sassoli FazanAbstract:Introduction: In this study we evaluated the characteristics of the tibialis anterior muscle after sciatic nerve crush and treatment with low-level laser therapy (LLLT) or the protein from Natural Latex (P1). Methods: We studied the following 6 groups of male Wistar rats: control (CG); exposed nerve (EG); injured nerve (IG); injured nerve with LLLT (LG); injured nerve with P1 (PG); and injured nerve with P1 and LLLT (LPG). Results: After 4 weeks, muscle morphology showed improvement in the treated groups; after 8 weeks, the treated groups resembled controls, especially the PG. Morphometry revealed muscle fiber atrophy after nerve injury, with time-dependent recovery. Histochemical analysis revealed increased intermediate fiber area. The PG was more similar to controls with NADH staining, whereas the LPG more closely resembled controls with SDH staining. Conclusion: Treatment using only P1 proved most efficient, revealing a negative interaction between P1 and LLLT. Muscle Nerve 52: 869–875, 2015
F Lavaud - One of the best experts on this subject based on the ideXlab platform.
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allergy to Latex avocado pear and banana evidence for a 30 kd antigen in immunoblotting
The Journal of Allergy and Clinical Immunology, 1995Co-Authors: F Lavaud, A Prevost, Claude Cossart, L Guerin, J Bernard, Serge KochmanAbstract:Abstract Allergens of Natural Latex, Latex gloves, avocado pear, and banana extracts were investigated by an immunoblotting technique in sera of patients experiencing associated Latex and fruit allergies. Extracts were separated by sodium dodecyl-sulfate–polyacrylamide gel electrophoresis and electroblotted onto nitrocellulose. After incubation with patients' sera, IgE antibodies were revealed by a goat anti-human IgE alkaline-phosphatase conjugate. Seventeen serum samples from patients with well-documented Latex allergy were studied. Among these patients, 10 demonstrated an allergy to avocado pear sometimes associated with banana. In sera from patients with Latex and fruit allergy, prominent IgE binding was revealed at about 30 kd with Latex and fruit extracts. Serum controls remained negative. Cross-inhibition of immunoblotting confirmed that this main allergen is linked to a common epitope present in Latex and fruits. This must be related to clinical findings and previous observations of cross-reactivity. (J ALLERGY CLIN IMMUNOL1995;95:557-64)
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allergy to Latex avocado pear and banana evidence for a 30 kd antigen in immunoblotting
The Journal of Allergy and Clinical Immunology, 1995Co-Authors: F Lavaud, A Prevost, Claude Cossart, L Guerin, J Bernard, Serge KochmanAbstract:Abstract Allergens of Natural Latex, Latex gloves, avocado pear, and banana extracts were investigated by an immunoblotting technique in sera of patients experiencing associated Latex and fruit allergies. Extracts were separated by sodium dodecyl-sulfate–polyacrylamide gel electrophoresis and electroblotted onto nitrocellulose. After incubation with patients' sera, IgE antibodies were revealed by a goat anti-human IgE alkaline-phosphatase conjugate. Seventeen serum samples from patients with well-documented Latex allergy were studied. Among these patients, 10 demonstrated an allergy to avocado pear sometimes associated with banana. In sera from patients with Latex and fruit allergy, prominent IgE binding was revealed at about 30 kd with Latex and fruit extracts. Serum controls remained negative. Cross-inhibition of immunoblotting confirmed that this main allergen is linked to a common epitope present in Latex and fruits. This must be related to clinical findings and previous observations of cross-reactivity. (J ALLERGY CLIN IMMUNOL1995;95:557-64)
