Nectria

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Kerry Odonnell - One of the best experts on this subject based on the ideXlab platform.

Nanang Rudianto Ariefta - One of the best experts on this subject based on the ideXlab platform.

  • new isocoumarins naphthoquinones and a cleistanthane type diterpene from Nectria pseudotrichia 120 1np
    Fitoterapia, 2018
    Co-Authors: Nanang Rudianto Ariefta, Priska Kristiana, Keitaro Tawaraya, Tetsuya Murayama, Takuya Koseki, Kenichi Kimura, Takako Aboshi, Naoaki Kurisawa, Yoshihito Shiono
    Abstract:

    Abstract Four new compounds, namely, Nectriapyrones A (2) and B (3), Nectriaquinone B (5), and zythiostromic acid C (8), were isolated from the brown rice culture of Nectria pseudotrichia 120-1NP together with four known compounds (1, 4, 6, and 7). To the best of our knowledge, this is the first report of 4 from a natural source. Their structures were determined on the basis of 1D/2D-NMR spectroscopy and HRESITOFMS data. In addition, the absolute configuration of secondary alcohols in 8 were determined using modified Mosher's ester method. All isolated compounds were evaluated for their antimicrobials activity, phytotoxicity, and cytotoxicity.

  • Nectrianolins a b and c new metabolites produced by endophytic fungus Nectria pseudotrichia 120 1np
    Tetrahedron Letters, 2017
    Co-Authors: Nanang Rudianto Ariefta, Priska Kristiana, Handojo Hadi Nurjanto, Hiroyuki Momma, Eunsang Kwon, Tatsuya Ashitani, Keitaro Tawaraya, Tetsuya Murayama
    Abstract:

    Abstract Two sesquiterpene-epoxycyclohexenone conjugates, Nectrianolins A ( 1 ) and B ( 2 ), together with a sesquiterpene, Nectrianolin C ( 3 ), were isolated from the brown rice culture of Nectria pseudotrichia 120-1NP, an endophytic fungus isolated from Gliricidia sepium . Their structures were determined on the basis of 1D-/2D-NMR spectroscopy and HRESIMS data analyses in combination with chemical means. Nectrianolins A–C ( 1 – 3 ) exhibited cytotoxic activity against both HL60 and HeLa cells.

Tetsuya Murayama - One of the best experts on this subject based on the ideXlab platform.

  • new isocoumarins naphthoquinones and a cleistanthane type diterpene from Nectria pseudotrichia 120 1np
    Fitoterapia, 2018
    Co-Authors: Nanang Rudianto Ariefta, Priska Kristiana, Keitaro Tawaraya, Tetsuya Murayama, Takuya Koseki, Kenichi Kimura, Takako Aboshi, Naoaki Kurisawa, Yoshihito Shiono
    Abstract:

    Abstract Four new compounds, namely, Nectriapyrones A (2) and B (3), Nectriaquinone B (5), and zythiostromic acid C (8), were isolated from the brown rice culture of Nectria pseudotrichia 120-1NP together with four known compounds (1, 4, 6, and 7). To the best of our knowledge, this is the first report of 4 from a natural source. Their structures were determined on the basis of 1D/2D-NMR spectroscopy and HRESITOFMS data. In addition, the absolute configuration of secondary alcohols in 8 were determined using modified Mosher's ester method. All isolated compounds were evaluated for their antimicrobials activity, phytotoxicity, and cytotoxicity.

  • Nectrianolins a b and c new metabolites produced by endophytic fungus Nectria pseudotrichia 120 1np
    Tetrahedron Letters, 2017
    Co-Authors: Nanang Rudianto Ariefta, Priska Kristiana, Handojo Hadi Nurjanto, Hiroyuki Momma, Eunsang Kwon, Tatsuya Ashitani, Keitaro Tawaraya, Tetsuya Murayama
    Abstract:

    Abstract Two sesquiterpene-epoxycyclohexenone conjugates, Nectrianolins A ( 1 ) and B ( 2 ), together with a sesquiterpene, Nectrianolin C ( 3 ), were isolated from the brown rice culture of Nectria pseudotrichia 120-1NP, an endophytic fungus isolated from Gliricidia sepium . Their structures were determined on the basis of 1D-/2D-NMR spectroscopy and HRESIMS data analyses in combination with chemical means. Nectrianolins A–C ( 1 – 3 ) exhibited cytotoxic activity against both HL60 and HeLa cells.

Priska Kristiana - One of the best experts on this subject based on the ideXlab platform.

  • new isocoumarins naphthoquinones and a cleistanthane type diterpene from Nectria pseudotrichia 120 1np
    Fitoterapia, 2018
    Co-Authors: Nanang Rudianto Ariefta, Priska Kristiana, Keitaro Tawaraya, Tetsuya Murayama, Takuya Koseki, Kenichi Kimura, Takako Aboshi, Naoaki Kurisawa, Yoshihito Shiono
    Abstract:

    Abstract Four new compounds, namely, Nectriapyrones A (2) and B (3), Nectriaquinone B (5), and zythiostromic acid C (8), were isolated from the brown rice culture of Nectria pseudotrichia 120-1NP together with four known compounds (1, 4, 6, and 7). To the best of our knowledge, this is the first report of 4 from a natural source. Their structures were determined on the basis of 1D/2D-NMR spectroscopy and HRESITOFMS data. In addition, the absolute configuration of secondary alcohols in 8 were determined using modified Mosher's ester method. All isolated compounds were evaluated for their antimicrobials activity, phytotoxicity, and cytotoxicity.

  • Nectrianolins a b and c new metabolites produced by endophytic fungus Nectria pseudotrichia 120 1np
    Tetrahedron Letters, 2017
    Co-Authors: Nanang Rudianto Ariefta, Priska Kristiana, Handojo Hadi Nurjanto, Hiroyuki Momma, Eunsang Kwon, Tatsuya Ashitani, Keitaro Tawaraya, Tetsuya Murayama
    Abstract:

    Abstract Two sesquiterpene-epoxycyclohexenone conjugates, Nectrianolins A ( 1 ) and B ( 2 ), together with a sesquiterpene, Nectrianolin C ( 3 ), were isolated from the brown rice culture of Nectria pseudotrichia 120-1NP, an endophytic fungus isolated from Gliricidia sepium . Their structures were determined on the basis of 1D-/2D-NMR spectroscopy and HRESIMS data analyses in combination with chemical means. Nectrianolins A–C ( 1 – 3 ) exhibited cytotoxic activity against both HL60 and HeLa cells.

Cameron R Currie - One of the best experts on this subject based on the ideXlab platform.

  • cycloheximide producing streptomyces associated with xyleborinus saxesenii and xyleborus affinis fungus farming ambrosia beetles
    Frontiers in Microbiology, 2020
    Co-Authors: Kirk J Grubbs, Jon Clardy, Frank Surup, Peter H W Biedermann, Bradon R Mcdonald, Jonathan L Klassen, Caitlin M Carlson, Cameron R Currie
    Abstract:

    Symbiotic microbes help a myriad of insects acquire nutrients. Recent work suggests that insects also frequently associate with actinobacterial symbionts that produce molecules to help defend against parasites and predators. Here we explore a potential association between Actinobacteria and two species of fungus-farming ambrosia beetles, Xyleborinus saxesenii and Xyleborus affinis. We isolated and identified actinobacterial and fungal symbionts from laboratory reared nests, and characterized small molecules produced by the putative actinobacterial symbionts. One 16S rRNA phylotype of Streptomyces (XylebKG-1) was abundantly and consistently isolated from the galleries and adults of X. saxesenii and X. affinis nests. In addition to Raffaelea sulphurea, the symbiont that X. saxesenii cultivates, we also repeatedly isolated a strain of Nectria sp. that is an antagonist of this mutualism. Inhibition bioassays between Streptomyces griseus XylebKG-1 and the fungal symbionts from X. saxesenii revealed strong inhibitory activity of the actinobacterium toward the fungal antagonist Nectria sp. but not the fungal mutualist R. sulphurea. Bioassay guided HPLC fractionation of S. griseus XylebKG-1 culture extracts, followed by NMR and mass spectrometry, identified cycloheximide as the compound responsible for the observed growth inhibition. A biosynthetic gene cluster putatively encoding cycloheximide was also identified in S. griseus XylebKG-1. The consistent isolation of a single 16S phylotype of Streptomyces from two species of ambrosia beetles, and our finding that a representative isolate of this phylotype produces cycloheximide, which inhibits a parasite of the system but not the cultivated fungus, suggests that these actinobacteria may play defensive roles within these systems.

  • cycloheximide producing streptomyces associated with xyleborinus saxesenii and xyleborus affinis fungus farming ambrosia beetles
    bioRxiv, 2019
    Co-Authors: Kirk J Grubbs, Jon Clardy, Frank Surup, Peter H W Biedermann, Bradon R Mcdonald, Jonathan L Klassen, Caitlin M Carlson, Cameron R Currie
    Abstract:

    Abstract Symbiotic microbes help a myriad of insects acquire nutrients. Recent work suggests that insects also frequently associate with actinobacterial symbionts that produce molecules to help defend against parasites and predators. Here we explore a potential association between Actinobacteria and two species of fungus-farming ambrosia beetles, Xyleborinus saxesenii and Xyleborus affinis. We isolated and identified actinobacterial and fungal symbionts from laboratory reared nests, and characterized small molecules produced by the putative actinobacterial symbionts. One 16S rRNA phylotype of Streptomyces (XylebKG-1) was abundantly and consistently isolated from the nests and adults of X. saxesenii and X. affinis nests. In addition to Raffaelea sulphurea, the symbiont that X. saxesenii cultivates, we also repeatedly isolated a strain of Nectria sp. that is an antagonist of this mutualism. Inhibition bioassays between S. griseus XylebKG-1 and the fungal symbionts from X. saxesenii revealed strong inhibitory activity of the actinobacterium towards the fungal antagonist Nectria sp. but not the fungal mutualist R. sulphurea. Bioassay guided HPLC fractionation of S. griseus XylebKG-1 culture extracts, followed by NMR and mass spectrometry identified cycloheximide as the compound responsible for the observed growth inhibition. A biosynthetic gene cluster putatively encoding cycloheximide was also identified in S. griseus XylebKG-1. The consistent isolation of a single 16S phylotype of Streptomyces from two species of ambrosia beetles, and our finding that a representative isolate of this phylotype produces cycloheximide, which inhibits a parasite of the system but not the cultivated fungus, suggests that these actinobacteria may play defensive roles within these systems.