The Experts below are selected from a list of 37947 Experts worldwide ranked by ideXlab platform
Lesley Mcgee - One of the best experts on this subject based on the ideXlab platform.
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laboratory methods for determining etiology of Neonatal Infection at population based sites in south asia the anisa study
Pediatric Infectious Disease Journal, 2016Co-Authors: Samir K Saha, Mohammad Shahidul Islam, Shahida M Qureshi, Belal Hossain, Anita K M Zaidi, Joyanta K Modak, Hasan M Alemran, Maureen H Diaz, Maksuda Islam, Lesley McgeeAbstract:The Aetiology of Neonatal Infection in South Asia (ANISA) study aims to determine the etiology of Neonatal Infections in 5 population-based sites in Bangladesh, India and Pakistan.The main laboratory challenges in ANISA were selection and consistent implementation of laboratory methods at participating sites with varied infrastructure. The other specific challenges included (1) specimen collection and transport to designated study laboratories and timely processing in rural settings; (2) minimal or nonexistent laboratory facilities at the field sites; (3) obtaining sufficient volumes of blood from enrolled infants aged 0-59 days and (4) caregivers' concerns about collection of clinical specimens from young infants. An additional challenge was selecting an appropriate molecular platform from multiple available options, all with limited field validation, for use in determining Infection in young infants.This article describes how the challenges of specimen collection, transport and processing and implementation of laboratory methods have been addressed in the ANISA study. It also describes the measures taken to improve detection of microorganisms causing young infant Infections by enhancing the sensitivity of existing laboratory methods for pathogen detection.
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laboratory methods for determining etiology of Neonatal Infection at population based sites in south asia the anisa study
Pediatric Infectious Disease Journal, 2016Co-Authors: Samir K Saha, Mohammad Shahidul Islam, Shahida M Qureshi, Belal Hossain, Anita K M Zaidi, Joyanta K Modak, Hasan M Alemran, Maureen H Diaz, Maksuda Islam, Lesley McgeeAbstract:Background:The Aetiology of Neonatal Infection in South Asia (ANISA) study aims to determine the etiology of Neonatal Infections in 5 population-based sites in Bangladesh, India and Pakistan.Methods:The main laboratory challenges in ANISA were selection and consistent implementation of laboratory me
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optimization of multiple pathogen detection using the taqman array card application for a population based study of Neonatal Infection
PLOS ONE, 2013Co-Authors: Maureen H Diaz, Jessica L Waller, Rebecca Napoliello, Md Shahidul Islam, Bernard J Wolff, Daniel J Burken, Rhiannon L Holden, Velusamy Srinivasan, Melissa L Arvay, Lesley McgeeAbstract:Identification of etiology remains a significant challenge in the diagnosis of infectious diseases, particularly in resource-poor settings. Viral, bacterial, and fungal pathogens, as well as parasites, play a role for many syndromes, and optimizing a single diagnostic system to detect a range of pathogens is challenging. The TaqMan Array Card (TAC) is a multiple-pathogen detection method that has previously been identified as a valuable technique for determining etiology of Infections and holds promise for expanded use in clinical microbiology laboratories and surveillance studies. We selected TAC for use in the Aetiology of Neonatal Infection in South Asia (ANISA) study for identifying etiologies of severe disease in neonates in Bangladesh, India, and Pakistan. Here we report optimization of TAC to improve pathogen detection and overcome technical challenges associated with use of this technology in a large-scale surveillance study. Specifically, we increased the number of assay replicates, implemented a more robust RT-qPCR enzyme formulation, and adopted a more efficient method for extraction of total nucleic acid from blood specimens. We also report the development and analytical validation of ten new assays for use in the ANISA study. Based on these data, we revised the study-specific TACs for detection of 22 pathogens in NP/OP swabs and 12 pathogens in blood specimens as well as two control reactions (internal positive control and human nucleic acid control) for each specimen type. The cumulative improvements realized through these optimization studies will benefit ANISA and perhaps other studies utilizing multiple-pathogen detection approaches. These lessons may also contribute to the expansion of TAC technology to the clinical setting.
Samir K Saha - One of the best experts on this subject based on the ideXlab platform.
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Strengthening the Reporting of Observational Studies in Epidemiology for Newborn Infection (STROBE-NI): an extension of the STROBE statement for Neonatal Infection research.
The Lancet. Infectious diseases, 2016Co-Authors: Elizabeth J A Fitchett, Samir K Saha, Anna C Seale, Stefania Vergnano, Mike Sharland, Paul T. Heath, Ramesh Agarwal, Adejumoke I. Ayede, Zulfiqar A Bhutta, Robert E. BlackAbstract:Neonatal Infections are estimated to account for a quarter of the 2·8 million annual Neonatal deaths, as well as approximately 3% of all disability-adjusted life-years. Despite this burden, few data are available on incidence, aetiology, and outcomes, particularly regarding impairment. We aimed to develop guidelines for improved scientific reporting of observational Neonatal Infection studies, to increase comparability and to strengthen research in this area. This checklist, Strengthening the Reporting of Observational Studies in Epidemiology for Newborn Infection (STROBE- NI), is an extension of the STROBE (Strengthening the Reporting of Observational Studies in Epidemiology) statement. STROBE-NI was developed following systematic reviews of published literature (1996-2015), compilation of more than 130 potential reporting recommendations, and circulation of a survey to relevant professionals worldwide, eliciting responses from 147 professionals from 37 countries. An international consensus meeting of 18 participants (with expertise in infectious diseases, neonatology, microbiology, epidemiology, and statistics) identified priority recommendations for reporting, additional to the STROBE statement. Implementation of these STROBE-NI recommendations, and linked checklist, aims to improve scientific reporting of Neonatal Infection studies, increasing data utility and allowing meta-analyses and pathogen-specific burden estimates to inform global policy and new interventions, including maternal vaccines.
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laboratory methods for determining etiology of Neonatal Infection at population based sites in south asia the anisa study
Pediatric Infectious Disease Journal, 2016Co-Authors: Samir K Saha, Mohammad Shahidul Islam, Shahida M Qureshi, Belal Hossain, Anita K M Zaidi, Joyanta K Modak, Hasan M Alemran, Maureen H Diaz, Maksuda Islam, Lesley McgeeAbstract:The Aetiology of Neonatal Infection in South Asia (ANISA) study aims to determine the etiology of Neonatal Infections in 5 population-based sites in Bangladesh, India and Pakistan.The main laboratory challenges in ANISA were selection and consistent implementation of laboratory methods at participating sites with varied infrastructure. The other specific challenges included (1) specimen collection and transport to designated study laboratories and timely processing in rural settings; (2) minimal or nonexistent laboratory facilities at the field sites; (3) obtaining sufficient volumes of blood from enrolled infants aged 0-59 days and (4) caregivers' concerns about collection of clinical specimens from young infants. An additional challenge was selecting an appropriate molecular platform from multiple available options, all with limited field validation, for use in determining Infection in young infants.This article describes how the challenges of specimen collection, transport and processing and implementation of laboratory methods have been addressed in the ANISA study. It also describes the measures taken to improve detection of microorganisms causing young infant Infections by enhancing the sensitivity of existing laboratory methods for pathogen detection.
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laboratory methods for determining etiology of Neonatal Infection at population based sites in south asia the anisa study
Pediatric Infectious Disease Journal, 2016Co-Authors: Samir K Saha, Mohammad Shahidul Islam, Shahida M Qureshi, Belal Hossain, Anita K M Zaidi, Joyanta K Modak, Hasan M Alemran, Maureen H Diaz, Maksuda Islam, Lesley McgeeAbstract:Background:The Aetiology of Neonatal Infection in South Asia (ANISA) study aims to determine the etiology of Neonatal Infections in 5 population-based sites in Bangladesh, India and Pakistan.Methods:The main laboratory challenges in ANISA were selection and consistent implementation of laboratory me
Maureen H Diaz - One of the best experts on this subject based on the ideXlab platform.
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laboratory methods for determining etiology of Neonatal Infection at population based sites in south asia the anisa study
Pediatric Infectious Disease Journal, 2016Co-Authors: Samir K Saha, Mohammad Shahidul Islam, Shahida M Qureshi, Belal Hossain, Anita K M Zaidi, Joyanta K Modak, Hasan M Alemran, Maureen H Diaz, Maksuda Islam, Lesley McgeeAbstract:The Aetiology of Neonatal Infection in South Asia (ANISA) study aims to determine the etiology of Neonatal Infections in 5 population-based sites in Bangladesh, India and Pakistan.The main laboratory challenges in ANISA were selection and consistent implementation of laboratory methods at participating sites with varied infrastructure. The other specific challenges included (1) specimen collection and transport to designated study laboratories and timely processing in rural settings; (2) minimal or nonexistent laboratory facilities at the field sites; (3) obtaining sufficient volumes of blood from enrolled infants aged 0-59 days and (4) caregivers' concerns about collection of clinical specimens from young infants. An additional challenge was selecting an appropriate molecular platform from multiple available options, all with limited field validation, for use in determining Infection in young infants.This article describes how the challenges of specimen collection, transport and processing and implementation of laboratory methods have been addressed in the ANISA study. It also describes the measures taken to improve detection of microorganisms causing young infant Infections by enhancing the sensitivity of existing laboratory methods for pathogen detection.
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laboratory methods for determining etiology of Neonatal Infection at population based sites in south asia the anisa study
Pediatric Infectious Disease Journal, 2016Co-Authors: Samir K Saha, Mohammad Shahidul Islam, Shahida M Qureshi, Belal Hossain, Anita K M Zaidi, Joyanta K Modak, Hasan M Alemran, Maureen H Diaz, Maksuda Islam, Lesley McgeeAbstract:Background:The Aetiology of Neonatal Infection in South Asia (ANISA) study aims to determine the etiology of Neonatal Infections in 5 population-based sites in Bangladesh, India and Pakistan.Methods:The main laboratory challenges in ANISA were selection and consistent implementation of laboratory me
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optimization of multiple pathogen detection using the taqman array card application for a population based study of Neonatal Infection
PLOS ONE, 2013Co-Authors: Maureen H Diaz, Jessica L Waller, Rebecca Napoliello, Md Shahidul Islam, Bernard J Wolff, Daniel J Burken, Rhiannon L Holden, Velusamy Srinivasan, Melissa L Arvay, Lesley McgeeAbstract:Identification of etiology remains a significant challenge in the diagnosis of infectious diseases, particularly in resource-poor settings. Viral, bacterial, and fungal pathogens, as well as parasites, play a role for many syndromes, and optimizing a single diagnostic system to detect a range of pathogens is challenging. The TaqMan Array Card (TAC) is a multiple-pathogen detection method that has previously been identified as a valuable technique for determining etiology of Infections and holds promise for expanded use in clinical microbiology laboratories and surveillance studies. We selected TAC for use in the Aetiology of Neonatal Infection in South Asia (ANISA) study for identifying etiologies of severe disease in neonates in Bangladesh, India, and Pakistan. Here we report optimization of TAC to improve pathogen detection and overcome technical challenges associated with use of this technology in a large-scale surveillance study. Specifically, we increased the number of assay replicates, implemented a more robust RT-qPCR enzyme formulation, and adopted a more efficient method for extraction of total nucleic acid from blood specimens. We also report the development and analytical validation of ten new assays for use in the ANISA study. Based on these data, we revised the study-specific TACs for detection of 22 pathogens in NP/OP swabs and 12 pathogens in blood specimens as well as two control reactions (internal positive control and human nucleic acid control) for each specimen type. The cumulative improvements realized through these optimization studies will benefit ANISA and perhaps other studies utilizing multiple-pathogen detection approaches. These lessons may also contribute to the expansion of TAC technology to the clinical setting.
Anita K M Zaidi - One of the best experts on this subject based on the ideXlab platform.
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laboratory methods for determining etiology of Neonatal Infection at population based sites in south asia the anisa study
Pediatric Infectious Disease Journal, 2016Co-Authors: Samir K Saha, Mohammad Shahidul Islam, Shahida M Qureshi, Belal Hossain, Anita K M Zaidi, Joyanta K Modak, Hasan M Alemran, Maureen H Diaz, Maksuda Islam, Lesley McgeeAbstract:The Aetiology of Neonatal Infection in South Asia (ANISA) study aims to determine the etiology of Neonatal Infections in 5 population-based sites in Bangladesh, India and Pakistan.The main laboratory challenges in ANISA were selection and consistent implementation of laboratory methods at participating sites with varied infrastructure. The other specific challenges included (1) specimen collection and transport to designated study laboratories and timely processing in rural settings; (2) minimal or nonexistent laboratory facilities at the field sites; (3) obtaining sufficient volumes of blood from enrolled infants aged 0-59 days and (4) caregivers' concerns about collection of clinical specimens from young infants. An additional challenge was selecting an appropriate molecular platform from multiple available options, all with limited field validation, for use in determining Infection in young infants.This article describes how the challenges of specimen collection, transport and processing and implementation of laboratory methods have been addressed in the ANISA study. It also describes the measures taken to improve detection of microorganisms causing young infant Infections by enhancing the sensitivity of existing laboratory methods for pathogen detection.
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laboratory methods for determining etiology of Neonatal Infection at population based sites in south asia the anisa study
Pediatric Infectious Disease Journal, 2016Co-Authors: Samir K Saha, Mohammad Shahidul Islam, Shahida M Qureshi, Belal Hossain, Anita K M Zaidi, Joyanta K Modak, Hasan M Alemran, Maureen H Diaz, Maksuda Islam, Lesley McgeeAbstract:Background:The Aetiology of Neonatal Infection in South Asia (ANISA) study aims to determine the etiology of Neonatal Infections in 5 population-based sites in Bangladesh, India and Pakistan.Methods:The main laboratory challenges in ANISA were selection and consistent implementation of laboratory me
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Infection surveillance protocol for a multicountry population based study in south asia to determine the incidence etiology and risk factors for Infections among young infants of 0 to 59 days old
Pediatric Infectious Disease Journal, 2016Co-Authors: Mohammad Shariful Islam, Anita K M Zaidi, Zulfiqar A Bhutta, Abdullah H Baqui, Pinaki Panigrahi, Anuradha Bose, Sajid Bashir Soofi, Abdul Momin Kazi, Dipak Kumar Mitra, Rita IsaacAbstract:BACKGROUND: Insufficient knowledge of the etiology and risk factors for community-acquired Neonatal Infection in low-income countries is a barrier to designing appropriate intervention strategies for these settings to reduce the burden and treatment of young infant Infection. To address these gaps we are conducting the Aetiology of Neonatal Infection in South Asia (ANISA) study among young infants in Bangladesh India and Pakistan. The objectives of ANISA are to establish a comprehensive surveillance system for registering newborns in study catchment areas and collecting data on bacterial and viral etiology and associated risk factors for Infections among young infants aged 0-59 days. METHODS: We are conducting active surveillance in 1 peri-urban and 4 rural communities. During 2 years of surveillance we expect to enroll an estimated 66000 newborns within 7 days of their birth and to follow-up them until 59 days of age. Community health workers visit each young infant in the study area 3 times in the first week of life and once a week thereafter. During these visits community health workers assess the newborns using a clinical algorithm and refer young infants with signs of suspected Infection to health care facilities where study physicians reassess them and provide care if needed. On physician confirmation of suspected Infection blood and respiratory specimens are collected and tested to identify the etiologic agent. CONCLUSIONS: ANISA is one of the largest initiatives ever undertaken to understand the etiology of young infant Infection in low-income countries. The data generated from this surveillance will help guide evidence-based decision making to improve health care in similar settings.
David A Diloreto - One of the best experts on this subject based on the ideXlab platform.
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a 9 year old girl with herpes simplex virus type 2 acute retinal necrosis treated with intravitreal foscarnet
Ocular Immunology and Inflammation, 2007Co-Authors: John King, Mina M Chung, David A DiloretoAbstract:A 9-year-old girl presented with a 2-week history of redness in the left eye. Examination revealed vitritis, retinal whitening, vasculitis, and optic nerve head edema. Polymerase chain reaction testing of the aqueous fluid revealed herpes simplex virus type 2. The retinitis was controlled with intravenous acyclovir and intravitreal foscarnet. The clinical course was complicated by retinal neovascularization and vitreous hemorrhage, which was treated by pars plana vitrectomy and endolaser. While there are few case reports of herpes simplex virus type 2 retinitis in children, this one is unique for the following reasons: it is the first reported case of herpes simplex virus type 2 retinitis in a child less than 10 years old without a previous history of Neonatal Infection or central nervous system involvement; no other children have been reported to have been treated with intravitreal foscarnet; and retinal neovascularization complicated the recovery.
