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Ani Sulistyarsi - One of the best experts on this subject based on the ideXlab platform.
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Pola pita protein total wereng hijau (Nephotettix virescens) dan daun tanaman padi (Oryza sativa) yang terinfeksi virus tungro
2012Co-Authors: Ani Sulistyarsi, Suranto Suranto, Supriyadi SupriyadiAbstract:Sulistyarsi A, Suranto, Supriyadi. 2012. The total protein band pattern of the green leafhoppers (Nephotettix virescens) and the leaves of rice (Oryza sativa) infected by tungro virus. Bioteknologi 9: 14-17. Tungro virus is one of most important diseases of rice plants caused by double infection with RTBV and RTSV which is transmitted by Nephotettix virescens Distant. The interaction between host and virus-vector are still quitted difficult to understand. The aims of this study were: (i) to know the character of the total protein band pattern of rice plants infected with tungro virus compared to the health one, (ii) to look at the different between the band profiles of total protein of N. virescens that consume the host rice plants infected by tungro virus and that of the healthy rice plants. Total protein band profiles of rice plants were identified using SDS-PAGE. To extract the leaves, buffer merchapto-ethanol was used, while the sample extraction of green leafhoppers employed buffer PBS IX, and for staining the protein coomassie brilliant blue was used. Data were analyzed descriptively based on the score of the migration of the band (Rf). The results showed that the protein contains of every 0.5 g of healthy leaves and the infected by the virus were 0.567 g and 1.011 g respectively. Clear difference of the protein pattern was found in the healthy plant and the infected one. In general, the entire band in the infected plant was much thicker compared to the infected leaves. Protein bands with a higher quantity were expressed by the protein on the molecular weight of 108, and 117 kDa. These proteins are presumably from the group of I²-galactosidase and bovine serum albumin. The function of such proteins is still unknown, but it may be related to the plant’s responses to virus infection, because the protein did not appear in the healthy plants. The total protein content of both N. virescens which acquired the healthy leaves and the infected one were 0.1395 g and 0.1546 g respectively. Qualitatively, there was no significant difference of the protein expression in those vectors, but slightly thicker band were observed in the infected leaves.
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Pola pita protein total wereng hijau (Nephotettix virescens) dan daun tanaman padi (Oryza sativa) yang terinfeksi virus tungro
MBI & UNS Solo, 2012Co-Authors: Ani SulistyarsiAbstract:Sulistyarsi A, Suranto, Supriyadi. 2012. Pola pita protein total wereng hijau (Nephotettix virescens) dan daun tanaman padi (Oryza sativa) yang terinfeksi virus tungro. Bioteknologi 9: 14-17. Virus tungro merupakan salah satu penyakit penting tanaman padi disebabkan oleh infeksi ganda RTBV dan RTSV, yang disebarkan oleh Nephotettix virescens Distant. Interaksi antara inang dan vektor virus masih sulit dipahami. Penelitian ini bertujuan: (i) mengetahui karakter pola pita protein total tanaman padi yang terinfeksi virus tungro dan tanaman padi sehat, (ii) mengetahui karakter pola pita protein total N. virescens yang mengkonsumsi inang tanaman padi terinfeksi virus tungro dan tanaman padi sehat. Pola pita protein total tanaman padi diidentifikasi menggunakan metode elektroforesis dengan SDS-PAGE. Sampel daun diekstraks menggunakan buffer mercapto ethanol, sedangkan sampel wereng hijau diekstraks menggunakan buffer PBS IX; dan pewarnaan pita protein menggunakan coomassie brilliant blue. Data dianalisis secara deskriptif berdasarkan nilai migrasi pita (Rf). Hasil penelitian menunjukkan kadar protein 0,5 g daun tanaman padi sehat dan daun tanaman padi terinfeksi virus tungro masing-masing sebesar 0,567 μg dan 1,011 μg. Perbedaan pola pita protein secara jelas ditemukan di antara tanaman padi sehat dan terinfeksi. Pada umumnya, pola pita protein pada tanaman padi yang terinfeksi virus tungro lebih tebal dari pada tanaman padi sehat. Pita protein dengan kuantitas lebih tinggi diekspresikan pada protein dengan berat molekul 108, dan 117 kDa. Diduga protein ini dari kelompok β-galaktosidase dan bovine serum albumin. Fungsi protein tersebut belum diketahui, namun diduga berkaitan dengan respon tanaman terhadap infeksi virus, karena protein tersebut tidak muncul pada tanaman sehat. Kadar protein total wereng hijau yang mengkonsumsi daun tanaman padi sehat dan terinfeksi virus tungro masing-masing sebesar 0,1395 μg dan 0,1546 μg. Secara kualitatif, tidak terdapat perbedaan yang signifikan ekspresi protein pada inang, tetapi pada daun yang terinfeksi cenderung lebih tebal
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The total protein band profile of the green leafhoppers (Nephotettix virescens) and of the rice (Oryza sativa) infected by tungro virus
MBI & UNS Solo, 2012Co-Authors: Ani SulistyarsiAbstract:Sulistyarsi A, Suranto, Supriyadi. 2012. The total protein band pattern of the green leafhoppers (Nephotettix virescens) and the leaves of rice (Oryza sativa) infected by tungro virus. Nusantara Bioscience 4: 32-35. Tungro virus is one of most important diseases of rice plants caused by double infection with RTBV and RTSV which is transmitted by Nephotettix virescens Distant. The interaction between host and virus-vector are still quitted difficult to understand. The aims of this study were: (i) to know the character of the total protein band pattern of rice plants infected with tungro virus compared to the health one, (ii) to look at the different between the band profiles of total protein of N. virescens that consume the host rice plants infected by tungro virus and that of the healthy rice plants. Total protein band profiles of rice plants were identified using SDS-PAGE. To extract the leaves, buffer merchapto-ethanol was used, while the sample extraction of green leafhoppers employed buffer PBS IX, and for staining the protein coomassie brilliant blue was used. Data were analyzed descriptively based on the score of the migration of the band (Rf). The results showed that the protein contains of every 0.5 g of healthy leaves and the infected by the virus were 0.567 g and 1.011 g respectively. Clear difference of the protein pattern was found in the healthy plant and the infected one. In general, the entire band in the infected plant was much thicker compared to the infected leaves. Protein bands with a higher quantity were expressed by the protein on the molecular weight of 108, and 117 kDa. These proteins are presumably from the group of β-galactosidase and bovine serum albumin. The function of such proteins is still unknown, but it may be related to the plant’s responses to virus infection, because the protein did not appear in the healthy plants. The total protein content of both N. virescens which acquired the healthy leaves and the infected one were 0.1395 g and 0.1546 g respectively. Qualitatively, there was no significant difference of the protein expression in those vectors, but slightly thicker band were observed in the infected leaves
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the total protein band profile of the green leafhoppers Nephotettix virescens and the leaves of rice oryza sativa infected by tungro virus
Nusantara Bioscience, 2012Co-Authors: Ani Sulistyarsi, Suranto Suranto, Supriyadi SupriyadiAbstract:Abstract. Sulistyarsi A, Suranto, Supriyadi. 2012. The total protein band pattern of the green leafhoppers (Nephotettix virescens) and the leaves of rice (Oryza sativa) infected by tungro virus. Nusantara Bioscience 4: 32-35. Tungro virus is one of most important diseases of rice plants caused by double infection with RTBV and RTSV which is transmitted by Nephotettix virescens Distant. The interaction between host and virus-vector are still quitted difficult to understand. The aims of this study were: (i) to know the character of the total protein band pattern of rice plants infected with tungro virus compared to the health one, (ii) to look at the different between the band profiles of total protein of N. virescens that consume the host rice plants infected by tungro virus and that of the healthy rice plants. Total protein band profiles of rice plants were identified using SDS-PAGE. To extract the leaves, buffer mercapto-ethanol was used, while the sample extraction of green leafhoppers employed buffer PBS IX, and for staining the protein coomassie brilliant blue was used. Data were analyzed descriptively based on the score of the migration of the band (Rf). The results showed that the protein contains every 0.5 g of healthy leaves and the infected by the virus were 0.567 g and 1.011 g respectively. Clear difference of the protein pattern was found in the healthy plant and the infected one. In general, the entire band in the infected plant was much thicker compared to the infected leaves. Protein bands with a higher quantity were expressed by the protein on the molecular weight of 108 and 117 kDa. These proteins are presumably from the group of ?-galactosidase and bovine serum albumin. The function of such proteins is still unknown, but it may be related to the plant’s responses to virus infection, because the protein did not appear in the healthy plants. The total protein content of both N. virescens which acquired the healthy leaves and the infected one were 0.1395 g and 0.1546 g respectively. Qualitatively, there was no significant difference in the protein expression in those vectors, but slightly thicker band were observed in the infected leaves.
Supriyadi Supriyadi - One of the best experts on this subject based on the ideXlab platform.
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Pola pita protein total wereng hijau (Nephotettix virescens) dan daun tanaman padi (Oryza sativa) yang terinfeksi virus tungro
2012Co-Authors: Ani Sulistyarsi, Suranto Suranto, Supriyadi SupriyadiAbstract:Sulistyarsi A, Suranto, Supriyadi. 2012. The total protein band pattern of the green leafhoppers (Nephotettix virescens) and the leaves of rice (Oryza sativa) infected by tungro virus. Bioteknologi 9: 14-17. Tungro virus is one of most important diseases of rice plants caused by double infection with RTBV and RTSV which is transmitted by Nephotettix virescens Distant. The interaction between host and virus-vector are still quitted difficult to understand. The aims of this study were: (i) to know the character of the total protein band pattern of rice plants infected with tungro virus compared to the health one, (ii) to look at the different between the band profiles of total protein of N. virescens that consume the host rice plants infected by tungro virus and that of the healthy rice plants. Total protein band profiles of rice plants were identified using SDS-PAGE. To extract the leaves, buffer merchapto-ethanol was used, while the sample extraction of green leafhoppers employed buffer PBS IX, and for staining the protein coomassie brilliant blue was used. Data were analyzed descriptively based on the score of the migration of the band (Rf). The results showed that the protein contains of every 0.5 g of healthy leaves and the infected by the virus were 0.567 g and 1.011 g respectively. Clear difference of the protein pattern was found in the healthy plant and the infected one. In general, the entire band in the infected plant was much thicker compared to the infected leaves. Protein bands with a higher quantity were expressed by the protein on the molecular weight of 108, and 117 kDa. These proteins are presumably from the group of I²-galactosidase and bovine serum albumin. The function of such proteins is still unknown, but it may be related to the plant’s responses to virus infection, because the protein did not appear in the healthy plants. The total protein content of both N. virescens which acquired the healthy leaves and the infected one were 0.1395 g and 0.1546 g respectively. Qualitatively, there was no significant difference of the protein expression in those vectors, but slightly thicker band were observed in the infected leaves.
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the total protein band profile of the green leafhoppers Nephotettix virescens and the leaves of rice oryza sativa infected by tungro virus
Nusantara Bioscience, 2012Co-Authors: Ani Sulistyarsi, Suranto Suranto, Supriyadi SupriyadiAbstract:Abstract. Sulistyarsi A, Suranto, Supriyadi. 2012. The total protein band pattern of the green leafhoppers (Nephotettix virescens) and the leaves of rice (Oryza sativa) infected by tungro virus. Nusantara Bioscience 4: 32-35. Tungro virus is one of most important diseases of rice plants caused by double infection with RTBV and RTSV which is transmitted by Nephotettix virescens Distant. The interaction between host and virus-vector are still quitted difficult to understand. The aims of this study were: (i) to know the character of the total protein band pattern of rice plants infected with tungro virus compared to the health one, (ii) to look at the different between the band profiles of total protein of N. virescens that consume the host rice plants infected by tungro virus and that of the healthy rice plants. Total protein band profiles of rice plants were identified using SDS-PAGE. To extract the leaves, buffer mercapto-ethanol was used, while the sample extraction of green leafhoppers employed buffer PBS IX, and for staining the protein coomassie brilliant blue was used. Data were analyzed descriptively based on the score of the migration of the band (Rf). The results showed that the protein contains every 0.5 g of healthy leaves and the infected by the virus were 0.567 g and 1.011 g respectively. Clear difference of the protein pattern was found in the healthy plant and the infected one. In general, the entire band in the infected plant was much thicker compared to the infected leaves. Protein bands with a higher quantity were expressed by the protein on the molecular weight of 108 and 117 kDa. These proteins are presumably from the group of ?-galactosidase and bovine serum albumin. The function of such proteins is still unknown, but it may be related to the plant’s responses to virus infection, because the protein did not appear in the healthy plants. The total protein content of both N. virescens which acquired the healthy leaves and the infected one were 0.1395 g and 0.1546 g respectively. Qualitatively, there was no significant difference in the protein expression in those vectors, but slightly thicker band were observed in the infected leaves.
N.v. Krishnaiah - One of the best experts on this subject based on the ideXlab platform.
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Identification of green leafhopper [Nephotettix virescens (Distant.)] resistance genes in rice
Indian Journal of Genetics and Plant Breeding, 2007Co-Authors: G. Padmavathi, N.v. Krishnaiah, G. S. V. Prasad, Y. Kondala RaoAbstract:The genetics of resistance to green leafhopper, [Nephotettix virescens (Distant.)] was studied in four pre-release green leafhopper resistant rice varieties viz., IET 12356 (RP 2432-98-6-3), IET 13268 (HKR 91–102), IET 15359 (SPT 6858), IET 15120 (CRM 47). The parental lines, F1s and F2 populations derived from the crosses of resistant varieties with the susceptible variety, TN1 or Phalguna or Sona and inter crosses among resistant varieties were screened against Indian population of green leafhopper in greenhouse at Directorate of Rice Research, Hyderabad. The inheritance of resistance suggested that two dominant complementary genes governed resistance in IET 13268, a single recessive gene in IET 15359, two recessive genes in IET 15120 and a single dominant gene in IET 12356. The single dominant gene of IET 12356 was allelic to Glh 6 in IR 64.
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Genetic analysis of resistance to green leafhopper, Nephotettix virescens (Distant) in rice (Oryza sativa L.)
Indian Journal of Genetics and Plant Breeding, 2006Co-Authors: G. Padmavathi, N.v. Krishnaiah, G. S. V. PrasadAbstract:The genetics of resistance to green leafhopper Nephotettix virescens (Distant) in five rice (Oryza sativa L.) donors including two pre-release varieties with unknown genes for resistance viz., IET 13341 and IET 12175; three differentials with known genes viz., TAPL 796, Maddai Karuppan and Ptb 8 was studied. The donors, F1 hybrids and F2 populations from the crosses of donors with the susceptible variety, TN1 and inter crosses among resistant donors were screened against Indian population of green leafhopper in green house. Inheritance of resistance suggested that a single dominant gene governed resistance in IET 13341, IET 12175, TAPL 796 and Maddai Karuppan and a single recessive gene in Ptb 8. Allelic tests with known gene donors i.e., TAPL 796 and Maddai Karuppan showed that the dominant genes present in IET 13341 and IET 12175 were different and independent of G/h 6 and G/h 7 of TAPL 796 and Maddai Karuppan, respectively.
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Development of Resistance in Rice Brown Planthopper Nilaparvata lugens (Stai) and Green Leafhopper Nephotettix virescens (Dist) to Neem Formulation
Pesticide Research Journal, 2002Co-Authors: N.v. Krishnaiah, A S Rama Prasad, I. C. Pasalu, K. Mahesh Kumar, T. LingaiahAbstract:In green house studies, rice brown planthopper, Nilaparvata lugens (Stal) and green leaf hopper, Nephotettix virescens (Dist.) have been exposed to Neem gold 4 and monocrotophos at LC70 to LC80 concentrations once in each generation for 26 generations separately. The resultant strains of BPH and GLH have been tested for their resistance development and cross-resistance pattern at the end of 26 generations.
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Efficacy of insecticides against leafhopper Nephotettix virescens and rice tungro disease
Indian phytopathology, 2002Co-Authors: C. S. Reddy, N.v. Krishnaiah, A S Rama PrasadAbstract:Rice tungro disease (RTD) is the most important virus disease of rice, transmitted by the vector, green leafhopper (GLH), Nephotettix virescens. Common symptoms of the disease include yellowing of leaves, stunting and reduced tillering and in severe cases very few or no panicle emergence from affected crop. Evolving resistant varieties against the disease as well as its vector was met with a few successes. However, the acceptability of resistant varieties depend on duration, grain quality etc. Therefore farmers continue to depend on use of insecticides to kill the vector in order to contain the spread of RTD. There are earlier reports to evaluate the efficacy of insecticides against the GLH under glass house conditions (2, 7, 8). However, investigations to evaluate the efficacy of insecticides against the vector with simultaneous containment of RTD under field conditions were also earlier carried out (3, 4, 10, 11). The present investigation has been under taken at DRR farm, Rajendranagar, to study the efficacy of some new insecticides against GLH as well as on containment of RTD.
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Effect of Neem Formulations on Reproduction and Oviposition of Rice Hoppers Nilaparvata lugens, Sogatella furcifera and Nephotettix virescens
Pesticide Research Journal, 2001Co-Authors: K. Mahesh Kumar, N.v. Krishnaiah, I. C. Pasalu, T. Lingaiah, K. KrishnaiahAbstract:Three commercial neem formulations viz., Nimbecidine, NG4 and Neem Azal T/S each at 10 ppm azadirachtin concentration, were tested against brown planthopper (Nilaparvata lugens Stal), white-backed planthopper (Sogatella furcifera Horvath) and green leafhopper (Nephotettix virescens Distant) for reproductive inhibition. Antiovipositional activity of Nimbecidine and Neem Azal T/S containing 10 and 50 ppm azadirachtin concentrations were evaluated. All the formulations tested at all the concentrations have shown significant reproductive inhibitory effects, presumably by way of derailing the physiological mechanism of egg development and significant ovipositinal deterrency.
Syahrir Pakki - One of the best experts on this subject based on the ideXlab platform.
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variation in rice tungro virus transmission ability by green leafhopper Nephotettix virescens distant homoptera cicadellidae on rice resistant varieties
Indonesian Journal of Agricultural Science, 2013Co-Authors: Nyoman I Widiarta, Adolf Bastian, Syahrir PakkiAbstract:Green leafhopper (GLH), Nephotettix virescens, is the most efficient vector of rice tungro virus disease. The disease is endemic in some provinces of Indonesia and commonly con-trolled using resistant varieties. Resistance of rice varieties to tungro could be classified into resistance to a virus and a vector. The history of GLH resistant varieties adoption affected the GLH adaptation in an area. The study was conducted in the period of 2009-2011 to evaluate the resistance status of five GLH resistant rice variety groups (T0-T4) using survival and transmission test. The GLH populations were collected from 15 tungro endemic provinces in Indonesia. The GLH was then reared in the greenhouse before used for the test. The degree of resistance to tungro viruses was calculated by adding the value of survival (weight x score of survival rate) and virus transmission rate (weight x score of transmission rate). The weights for survival and transmission rate were set to 40 and 60, respectively. The results showed that the rank of resistant variety groups in decreasing order of resistance were T4, T1, T2 and T3. Five variations in GLH transmission efficiency were identified, i.e. 170, 070, 050, 030 and 010. GLH populations from Bali and West Nusa Tenggara were the most efficient vector for rice tungro virus. We concluded that there were diversities in the degree of resistance among GLH resistant varieties. Variation in virus transmission efficiency (biotype) among GLH populations collected from various tungro endemic areas closely related to the history of adoption of rice varieties.
Nyoman I Widiarta - One of the best experts on this subject based on the ideXlab platform.
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varian efikasi penularan tungro oleh koloni koloni wereng hijau Nephotettix virescens distant
Jurnal Entomologi Indonesia, 2017Co-Authors: Nyoman I Widiarta, Dede Kusdiaman, Sri S Siwi, Andi HasanuddinAbstract:Green leafhopper-resistant variety is one of rice tungro virus disease control measure. Green leafhopper (GLH), Nephotettix virescens is the most efficient vector of tungro. Among 7 sources of resistant gene to GLH 4 genes have been employed to breed resistant variety. Efficiency variant of GLH as indicated by their efficiency to transfer tungro virus was identified by inoculation test. GLH colonies were collected from tungro endemic areas in Java, Bali, West Nusa and South Sulawesi. Efficiency variant of GLH colony was characterized by their ability to transfer tungro virus to GLH-resistant variety with various source of resistant genes. Sources of tungro inoculum were obtained in Bogor. The results of the test showed that there was a variation in the ability of GLH colonies to tranfer tungro to various GLH-resistant variety, thus indicate there was a variant in GLH colony. The ability of GLH colonies to transfer virus ranked from high to low, were West Nusa Tenggara, Bali, East Java , South Sulawesi, D.I. Yogyakarta, West Java and Central Java. On the other hand GLH-resistant variety ranked from resistant to susceptible were varieties with resistant genes group glh4, Glh6, Glh1 and Glh5. Five variants colonies of GLH were successfully identified which named as colony 0050, 0000, 1050, 1650 and 1654. Biotype 0000 has the lowest ability to transfer virus but biotype 1654 efficiently transfer virus to all of GLH-resistant variety groups.
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variation in rice tungro virus transmission ability by green leafhopper Nephotettix virescens distant homoptera cicadellidae on rice resistant varieties
Indonesian Journal of Agricultural Science, 2013Co-Authors: Nyoman I Widiarta, Adolf Bastian, Syahrir PakkiAbstract:Green leafhopper (GLH), Nephotettix virescens, is the most efficient vector of rice tungro virus disease. The disease is endemic in some provinces of Indonesia and commonly con-trolled using resistant varieties. Resistance of rice varieties to tungro could be classified into resistance to a virus and a vector. The history of GLH resistant varieties adoption affected the GLH adaptation in an area. The study was conducted in the period of 2009-2011 to evaluate the resistance status of five GLH resistant rice variety groups (T0-T4) using survival and transmission test. The GLH populations were collected from 15 tungro endemic provinces in Indonesia. The GLH was then reared in the greenhouse before used for the test. The degree of resistance to tungro viruses was calculated by adding the value of survival (weight x score of survival rate) and virus transmission rate (weight x score of transmission rate). The weights for survival and transmission rate were set to 40 and 60, respectively. The results showed that the rank of resistant variety groups in decreasing order of resistance were T4, T1, T2 and T3. Five variations in GLH transmission efficiency were identified, i.e. 170, 070, 050, 030 and 010. GLH populations from Bali and West Nusa Tenggara were the most efficient vector for rice tungro virus. We concluded that there were diversities in the degree of resistance among GLH resistant varieties. Variation in virus transmission efficiency (biotype) among GLH populations collected from various tungro endemic areas closely related to the history of adoption of rice varieties.
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dinamika populasi Nephotettix virescens pada dua pola tanam padi sawah
Jurnal Perlindungan Tanaman Indonesia, 1999Co-Authors: Nyoman I Widiarta, Dede Kusdiaman, Andi HasanuddinAbstract:Planting pattern of irrigated rice in Indonesia can be categorized generally into consecutive rice-rice-rice and rice-rice-fallow/secondary crop patterns. A study was conducted in farmer’s fields in two planting patterns in 1997 and 1998 to elucidate population dynamics of green leafhopper, vector of tungro disease and factors affecting their dynamics. A susceptible rice variety to green leafhopper was planted three times in one season with monthly interval between planting time. The rice was planted following farmer usual practices except no insecticide was applied. The population of green leafhopper and their natural enemies was surveyed by sweeping net. The egg predator and parasitoid were observed by dissecting the rice stems and incubating the discovered eggs. Life table of the first generation was constructed. The key-factor analysis was conducted to identify the key-mortality factor by regression methods. Numerical response of natural enemies was also analyzed to know the relationship of key-mortality to predator. Population density of green leafhopper increased mainly during early stage of rice growth in the rice-rice-rice planting pattern, but in the rice-rice-fallow/secondary crop planting patterns showed almost no population increase at all. The nymph mortality including adult disappearance before maturation was the key mortality for the population in both planting patterns. However, there were different in population process between population in different planting patterns. In contrast to the rice-rice-secondary crop, there were no numerical response between nymphal mortality and predator density in the rice-rice-rice planting pattern. In the rice-rice-rice planting pattern adults dispersal played important role. Therefore to control tungro, reducing feeding and inoculation ability of green leafhopper was considered important. In the rice-rice-secondary crop, natural enemies conservation especially the predator was considered important.