The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Hanna Debiec - One of the best experts on this subject based on the ideXlab platform.
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neural epidermal growth factor like 1 protein nell 1 associated membranous Nephropathy
Kidney International, 2020Co-Authors: Sanjeev Sethi, Hanna Debiec, Benjamin J Madden, Cristine M Charlesworth, Johann Morelle, Lou Ann Gross, Aishwarya Ravindran, David BuobAbstract:Membranous Nephropathy is characterized by deposition of immune complexes along the glomerular basement membrane. PLA2R and THSD7A are target antigens in 70% and 1-5% of primary membranous Nephropathy cases, respectively. In the remaining cases, the target antigen is unknown. Here, laser microdissection of glomeruli followed by mass spectrometry was used to identify novel antigen(s) in PLA2R-negative membranous Nephropathy. An initial pilot mass spectrometry study in 35 cases of PLA2R-negative membranous Nephropathy showed high spectral counts for neural tissue encoding protein with EGF-like repeats, NELL-1, in six cases. Mass spectrometry failed to detect NELL-1 in 23 PLA2R-associated membranous Nephropathy and 88 controls. NELL-1 was localized by immunohistochemistry, which showed bright granular glomerular basement membrane staining for NELL-1 in all six cases. Next, an additional 23 NELL-1 positive cases of membranous Nephropathy were identified by immunohistochemistry in a discovery cohort of 91 PLA2R-negative membranous Nephropathy cases, 14 were confirmed by mass spectrometry. Thus, 29 of 126 PLA2R-negative cases were positive for NELL-1. PLA2R-associated membranous Nephropathy and controls stained negative for NELL-1. We then identified five NELL-1 positive cases of membranous Nephropathy out of 84 PLA2R and THSD7A-negative cases in two validation cohorts from France and Belgium. By confocal microscopy, both IgG and NELL-1 co-localized to the glomerular basement membrane. Western blot analysis showed reactivity to NELL-1 in five available sera, but no reactivity in control sera. Clinical and biopsy findings of NELL-1 positive membranous Nephropathy showed features of primary membranous Nephropathy. Thus, a subset of membranous Nephropathy is associated with accumulation and co-localization of NELL-1 and IgG along the glomerular basement membrane, and with anti-NELL-1 antibodies in the serum. Hence, NELL-1 defines a distinct type of primary membranous Nephropathy.
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pla2r autoantibodies and pla2r glomerular deposits in membranous Nephropathy
The New England Journal of Medicine, 2011Co-Authors: Hanna Debiec, Pierre RoncoAbstract:To the Editor: Membranous Nephropathy is a common cause of the nephrotic syndrome in adults. Treatment is controversial and challenging because of the heterogeneity of the disease and a lack of reliable biomarkers.1,2 M-type phospholipase A2 receptor (PLA2R) was recently identified as a major target antigen involved in idiopathic membranous Nephropathy in adults.3 Circulating autoantibodies against PLA2R were found in 70 to 80% of patients with idiopathic membranous Nephropathy but not in those with secondary membranous Nephropathy or other renal diseases. It has been suggested that the serum level of PLA2R autoantibody . . .
Alexandra J Lansky - One of the best experts on this subject based on the ideXlab platform.
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low hematocrit predicts contrast induced Nephropathy after percutaneous coronary interventions
Kidney International, 2005Co-Authors: Zoran Lasic, Gary S Mintz, Eugenia Nikolsky, Roxana Mehran, Alexandra J LanskyAbstract:Low hematocrit predicts contrast-induced Nephropathy after percutaneous coronary interventions. Background The relationship between low hematocrit and contrast-induced Nephropathy has not been investigated. Methods Of 6,773 consecutive patients treated with percutaneous coronary intervention, contrast-induced Nephropathy (an increase of ≥25% or ≥0.5mg/dL in preprocedure serum creatinine, at 48 hours postprocedure) occurred in 942 (13.9%) patients. Results Rates of contrast-induced Nephropathy steadily increased as baseline hematocrit quintile decreased (from 10.3% in the highest quintile to 23.3% in the lowest quintile) (χ 2 for trend, P P 5.9% had almost doubled rates of contrast-induced Nephropathy compared with patients with hematocrit change P Conclusion Lower hematocrit is an important risk factor for contrast-induced Nephropathy. Whether correcting the hematocrit prepercutaneous coronary intervention might decrease the rates of contrast-induced Nephropathy should be addressed in a prospectively designed trial.
David Buob - One of the best experts on this subject based on the ideXlab platform.
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neural epidermal growth factor like 1 protein nell 1 associated membranous Nephropathy
Kidney International, 2020Co-Authors: Sanjeev Sethi, Hanna Debiec, Benjamin J Madden, Cristine M Charlesworth, Johann Morelle, Lou Ann Gross, Aishwarya Ravindran, David BuobAbstract:Membranous Nephropathy is characterized by deposition of immune complexes along the glomerular basement membrane. PLA2R and THSD7A are target antigens in 70% and 1-5% of primary membranous Nephropathy cases, respectively. In the remaining cases, the target antigen is unknown. Here, laser microdissection of glomeruli followed by mass spectrometry was used to identify novel antigen(s) in PLA2R-negative membranous Nephropathy. An initial pilot mass spectrometry study in 35 cases of PLA2R-negative membranous Nephropathy showed high spectral counts for neural tissue encoding protein with EGF-like repeats, NELL-1, in six cases. Mass spectrometry failed to detect NELL-1 in 23 PLA2R-associated membranous Nephropathy and 88 controls. NELL-1 was localized by immunohistochemistry, which showed bright granular glomerular basement membrane staining for NELL-1 in all six cases. Next, an additional 23 NELL-1 positive cases of membranous Nephropathy were identified by immunohistochemistry in a discovery cohort of 91 PLA2R-negative membranous Nephropathy cases, 14 were confirmed by mass spectrometry. Thus, 29 of 126 PLA2R-negative cases were positive for NELL-1. PLA2R-associated membranous Nephropathy and controls stained negative for NELL-1. We then identified five NELL-1 positive cases of membranous Nephropathy out of 84 PLA2R and THSD7A-negative cases in two validation cohorts from France and Belgium. By confocal microscopy, both IgG and NELL-1 co-localized to the glomerular basement membrane. Western blot analysis showed reactivity to NELL-1 in five available sera, but no reactivity in control sera. Clinical and biopsy findings of NELL-1 positive membranous Nephropathy showed features of primary membranous Nephropathy. Thus, a subset of membranous Nephropathy is associated with accumulation and co-localization of NELL-1 and IgG along the glomerular basement membrane, and with anti-NELL-1 antibodies in the serum. Hence, NELL-1 defines a distinct type of primary membranous Nephropathy.
Johann Morelle - One of the best experts on this subject based on the ideXlab platform.
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neural epidermal growth factor like 1 protein nell 1 associated membranous Nephropathy
Kidney International, 2020Co-Authors: Sanjeev Sethi, Hanna Debiec, Benjamin J Madden, Cristine M Charlesworth, Johann Morelle, Lou Ann Gross, Aishwarya Ravindran, David BuobAbstract:Membranous Nephropathy is characterized by deposition of immune complexes along the glomerular basement membrane. PLA2R and THSD7A are target antigens in 70% and 1-5% of primary membranous Nephropathy cases, respectively. In the remaining cases, the target antigen is unknown. Here, laser microdissection of glomeruli followed by mass spectrometry was used to identify novel antigen(s) in PLA2R-negative membranous Nephropathy. An initial pilot mass spectrometry study in 35 cases of PLA2R-negative membranous Nephropathy showed high spectral counts for neural tissue encoding protein with EGF-like repeats, NELL-1, in six cases. Mass spectrometry failed to detect NELL-1 in 23 PLA2R-associated membranous Nephropathy and 88 controls. NELL-1 was localized by immunohistochemistry, which showed bright granular glomerular basement membrane staining for NELL-1 in all six cases. Next, an additional 23 NELL-1 positive cases of membranous Nephropathy were identified by immunohistochemistry in a discovery cohort of 91 PLA2R-negative membranous Nephropathy cases, 14 were confirmed by mass spectrometry. Thus, 29 of 126 PLA2R-negative cases were positive for NELL-1. PLA2R-associated membranous Nephropathy and controls stained negative for NELL-1. We then identified five NELL-1 positive cases of membranous Nephropathy out of 84 PLA2R and THSD7A-negative cases in two validation cohorts from France and Belgium. By confocal microscopy, both IgG and NELL-1 co-localized to the glomerular basement membrane. Western blot analysis showed reactivity to NELL-1 in five available sera, but no reactivity in control sera. Clinical and biopsy findings of NELL-1 positive membranous Nephropathy showed features of primary membranous Nephropathy. Thus, a subset of membranous Nephropathy is associated with accumulation and co-localization of NELL-1 and IgG along the glomerular basement membrane, and with anti-NELL-1 antibodies in the serum. Hence, NELL-1 defines a distinct type of primary membranous Nephropathy.
Sanjeev Sethi - One of the best experts on this subject based on the ideXlab platform.
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neural epidermal growth factor like 1 protein nell 1 associated membranous Nephropathy
Kidney International, 2020Co-Authors: Sanjeev Sethi, Hanna Debiec, Benjamin J Madden, Cristine M Charlesworth, Johann Morelle, Lou Ann Gross, Aishwarya Ravindran, David BuobAbstract:Membranous Nephropathy is characterized by deposition of immune complexes along the glomerular basement membrane. PLA2R and THSD7A are target antigens in 70% and 1-5% of primary membranous Nephropathy cases, respectively. In the remaining cases, the target antigen is unknown. Here, laser microdissection of glomeruli followed by mass spectrometry was used to identify novel antigen(s) in PLA2R-negative membranous Nephropathy. An initial pilot mass spectrometry study in 35 cases of PLA2R-negative membranous Nephropathy showed high spectral counts for neural tissue encoding protein with EGF-like repeats, NELL-1, in six cases. Mass spectrometry failed to detect NELL-1 in 23 PLA2R-associated membranous Nephropathy and 88 controls. NELL-1 was localized by immunohistochemistry, which showed bright granular glomerular basement membrane staining for NELL-1 in all six cases. Next, an additional 23 NELL-1 positive cases of membranous Nephropathy were identified by immunohistochemistry in a discovery cohort of 91 PLA2R-negative membranous Nephropathy cases, 14 were confirmed by mass spectrometry. Thus, 29 of 126 PLA2R-negative cases were positive for NELL-1. PLA2R-associated membranous Nephropathy and controls stained negative for NELL-1. We then identified five NELL-1 positive cases of membranous Nephropathy out of 84 PLA2R and THSD7A-negative cases in two validation cohorts from France and Belgium. By confocal microscopy, both IgG and NELL-1 co-localized to the glomerular basement membrane. Western blot analysis showed reactivity to NELL-1 in five available sera, but no reactivity in control sera. Clinical and biopsy findings of NELL-1 positive membranous Nephropathy showed features of primary membranous Nephropathy. Thus, a subset of membranous Nephropathy is associated with accumulation and co-localization of NELL-1 and IgG along the glomerular basement membrane, and with anti-NELL-1 antibodies in the serum. Hence, NELL-1 defines a distinct type of primary membranous Nephropathy.
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triamterene crystalline Nephropathy
American Journal of Kidney Diseases, 2014Co-Authors: Samih H Nasr, Dawn S Milliner, Thomas D Wooldridge, Sanjeev SethiAbstract:Medications can cause a tubulointerstitial insult leading to acute kidney injury through multiple mechanisms. Acute tubular injury, a dose-dependent process, occurs due to direct toxicity on tubular cells. Acute interstitial nephritis characterized by interstitial inflammation and tubulitis develops from drugs that incite an allergic reaction. Other less common mechanisms include osmotic nephrosis and crystalline Nephropathy. The latter complication is rare but has been associated with several drugs, such as sulfadiazine, indinavir, methotrexate, and ciprofloxacin. Triamterene crystalline Nephropathy has been reported only rarely, and its histologic characteristics are not well characterized. We report 2 cases of triamterene crystalline Nephropathy, one of which initially was misdiagnosed as 2,8-dihydroxyadenine crystalline Nephropathy.
