The Experts below are selected from a list of 2019 Experts worldwide ranked by ideXlab platform
Chang-hong Duan - One of the best experts on this subject based on the ideXlab platform.
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Effects of ginkgo biloba extract on the expression of inducible nitric oxide synthase and platelet-activating factor after local cerebral ischemia/reperfusion in rats
Chinese journal of experimental surgery, 2012Co-Authors: Chang-hong Duan, Chong-jun Wang, Dong-fang ZhaoAbstract:Objective To explore the protective effect and mechanism of ginkgo biloba extract (GBE) on brain tissue after local cerebral ischemia and reperfusion in rats.Methods Eight male SpragueDawley rats were randomly divided into two groups:control group ( group A,normal saline injected,n =40),GBE intervention group (group B,GBE injected,n =40),which were then divided into 4 subgrouops,namely,1 h,3 h,6 h and 24 h after reperfusion,10 in each sub-group.The model of right middle cerebral artery occlusion (MCAO) and reperfusion was made.Pathological changes in brain morphology were observed by Hematoxylin and Eosin (HE) staining; immunohitochemical method was used to detect the inducible nitric oxide synthase (iNOS) changes; platelet activating factor (PAF) was detected by enzyme linked immunosorbent assay (ELISA).Results Nerve Cell Necrosis,apoptosis and cytoplasmic vacuolatin were observed,neuronal shape was difficult to identify,and most of the Cell structures disappeared in group A.The injury was relieved at the same time points in group B compared with that in group A.Immunohitochemical results showed that there were a few iNOS expression positive Cells 1 h after reperfusion,which increased at 3 h after referfusion and had nucler translocation.Analysis by Image-pro plus 5.0 showed that iNOS expression levels in group B ( 1851.38 ±242.12,2005.41 ±290.52,2625.28 ±385.21,3142.50 ± 425.72) were significantly lower than those in group A ( 1950.25 ± 298.26,2232.45 ± 305.28,3251.22 ± 425.26,3965.36 ± 521.62) (P ＜ 0.05 ),respectively.ELISA results showed that serum PAF levels were increased after ischemia-reperfusion,peaked at 6 h,and decreased at 24 h.Serum PAF level were significantly lower in group B ( 15.36 ± 2.12,18.56 ± 3.28,28.21 ± 4.26,22.48 ± 4.21 ) μg/L than in group A (20.52 ± 4.26,28.25 ± 6.18,36.08 ± 7.45,30.26 ± 6.02) μg/L ( P ＜ 0.05).Conclusion iNOS,and PAF change significantly after the cerebral ischemia-reperfusion in rats,which may be involved in cerebral ischemia-reperfusion injury mechanism.GBE can significantly alleviate the cerebral injury by regulating the expression of iNOS and PAF,suggesting GBE can effectively protect the ischemic cerebral tissues. Key words: Ginkgo biloba extract; Cerebral ischemia; Reperfusion injury; Inducible nitric oxide synthase
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Effects of ginkgo biloba extract on the expression of bax, GFAP after local cerebral ischemia/reperfusion in rats
Chinese journal of experimental surgery, 2012Co-Authors: Chang-hong Duan, Chong-jun Wang, Chao-yuan ZhouAbstract:Objective To explore the protective action of ginkgo biloba extract (GBE) after local cerebral ischemia and reperfusion in rats.Methods 80 male Sprague-Dawley rats were randomly divided into two groups:control group ( group A,saline injected,n =40 ),GBE intervention group ( group GBE injected,n =40),which were divided into 4 subgroups,namely,reperfusion after 1,3,6 and 24 h groups,10 in each subgroup.The animal model of right middle cerebral artery occlusion (MCAO) and reperfusion was established; pathological changes in brain morphology were observed by Hematoxylin and Eosin (HE) staining; serum glial fibrillary acidic protein (GFAP) was detected by enzyme linked immunosorbent assay (ELISA) method; changes of bax mRNA expression was detected by reverse transcriptionpolymerase chain reaction (RT-PCR) method.Results (1) Nerve Cell Necrosis,apoptosis were observed,neuronal shape was difficult to identify,and most of the Cell structure disappeared in group A.While,the injury was milder at same time points in group B compared with group A.(2) ELISA results showed that serum GFAP levels increased after ischemia-reperfusion,the levels in group B (275.29 ±112.72,308.72 ± 124.11,372.56 ± 185.62,452.12 ± 145.26) ng/L were significantly lower than those in group A (437.17 ± 152.26,490.27 ± 198.37,583.45 ± 201.42,656.26 ± 256.36) ng/L,respectively( P ＜ 0.05 ).3.RT-PCR results showed that bax mRNA expression in group B (0.256 ± 0.018,0.302 ±0.023,0.417 ±0.034,0.527 ±0.052) was significantly higher than that in group A (0.333 ±0.021,0.452 ±0.037,0.587±0.052,0.726±0.046,respectively) (P＜0.05).Conclusion GFAP and bax change significantly after cerebral ischemia-reperfusion which may involve in cerebral isehemia reperfusion injury mechanism.GBE can significantly reduce the cerebral injury by down regulating the expression of GFAP and bax.GBE is effective on prevention of cerebral ischemia-reperfusion injury. Key words: Ginkgo biloba extract; Cerebral ischemia-reperfusion injury; Apoptosis; bax; Glial fibrillary acidic protein
Dong-fang Zhao - One of the best experts on this subject based on the ideXlab platform.
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Effects of ginkgo biloba extract on the expression of inducible nitric oxide synthase and platelet-activating factor after local cerebral ischemia/reperfusion in rats
Chinese journal of experimental surgery, 2012Co-Authors: Chang-hong Duan, Chong-jun Wang, Dong-fang ZhaoAbstract:Objective To explore the protective effect and mechanism of ginkgo biloba extract (GBE) on brain tissue after local cerebral ischemia and reperfusion in rats.Methods Eight male SpragueDawley rats were randomly divided into two groups:control group ( group A,normal saline injected,n =40),GBE intervention group (group B,GBE injected,n =40),which were then divided into 4 subgrouops,namely,1 h,3 h,6 h and 24 h after reperfusion,10 in each sub-group.The model of right middle cerebral artery occlusion (MCAO) and reperfusion was made.Pathological changes in brain morphology were observed by Hematoxylin and Eosin (HE) staining; immunohitochemical method was used to detect the inducible nitric oxide synthase (iNOS) changes; platelet activating factor (PAF) was detected by enzyme linked immunosorbent assay (ELISA).Results Nerve Cell Necrosis,apoptosis and cytoplasmic vacuolatin were observed,neuronal shape was difficult to identify,and most of the Cell structures disappeared in group A.The injury was relieved at the same time points in group B compared with that in group A.Immunohitochemical results showed that there were a few iNOS expression positive Cells 1 h after reperfusion,which increased at 3 h after referfusion and had nucler translocation.Analysis by Image-pro plus 5.0 showed that iNOS expression levels in group B ( 1851.38 ±242.12,2005.41 ±290.52,2625.28 ±385.21,3142.50 ± 425.72) were significantly lower than those in group A ( 1950.25 ± 298.26,2232.45 ± 305.28,3251.22 ± 425.26,3965.36 ± 521.62) (P ＜ 0.05 ),respectively.ELISA results showed that serum PAF levels were increased after ischemia-reperfusion,peaked at 6 h,and decreased at 24 h.Serum PAF level were significantly lower in group B ( 15.36 ± 2.12,18.56 ± 3.28,28.21 ± 4.26,22.48 ± 4.21 ) μg/L than in group A (20.52 ± 4.26,28.25 ± 6.18,36.08 ± 7.45,30.26 ± 6.02) μg/L ( P ＜ 0.05).Conclusion iNOS,and PAF change significantly after the cerebral ischemia-reperfusion in rats,which may be involved in cerebral ischemia-reperfusion injury mechanism.GBE can significantly alleviate the cerebral injury by regulating the expression of iNOS and PAF,suggesting GBE can effectively protect the ischemic cerebral tissues. Key words: Ginkgo biloba extract; Cerebral ischemia; Reperfusion injury; Inducible nitric oxide synthase
Chong-jun Wang - One of the best experts on this subject based on the ideXlab platform.
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Effects of ginkgo biloba extract on the expression of inducible nitric oxide synthase and platelet-activating factor after local cerebral ischemia/reperfusion in rats
Chinese journal of experimental surgery, 2012Co-Authors: Chang-hong Duan, Chong-jun Wang, Dong-fang ZhaoAbstract:Objective To explore the protective effect and mechanism of ginkgo biloba extract (GBE) on brain tissue after local cerebral ischemia and reperfusion in rats.Methods Eight male SpragueDawley rats were randomly divided into two groups:control group ( group A,normal saline injected,n =40),GBE intervention group (group B,GBE injected,n =40),which were then divided into 4 subgrouops,namely,1 h,3 h,6 h and 24 h after reperfusion,10 in each sub-group.The model of right middle cerebral artery occlusion (MCAO) and reperfusion was made.Pathological changes in brain morphology were observed by Hematoxylin and Eosin (HE) staining; immunohitochemical method was used to detect the inducible nitric oxide synthase (iNOS) changes; platelet activating factor (PAF) was detected by enzyme linked immunosorbent assay (ELISA).Results Nerve Cell Necrosis,apoptosis and cytoplasmic vacuolatin were observed,neuronal shape was difficult to identify,and most of the Cell structures disappeared in group A.The injury was relieved at the same time points in group B compared with that in group A.Immunohitochemical results showed that there were a few iNOS expression positive Cells 1 h after reperfusion,which increased at 3 h after referfusion and had nucler translocation.Analysis by Image-pro plus 5.0 showed that iNOS expression levels in group B ( 1851.38 ±242.12,2005.41 ±290.52,2625.28 ±385.21,3142.50 ± 425.72) were significantly lower than those in group A ( 1950.25 ± 298.26,2232.45 ± 305.28,3251.22 ± 425.26,3965.36 ± 521.62) (P ＜ 0.05 ),respectively.ELISA results showed that serum PAF levels were increased after ischemia-reperfusion,peaked at 6 h,and decreased at 24 h.Serum PAF level were significantly lower in group B ( 15.36 ± 2.12,18.56 ± 3.28,28.21 ± 4.26,22.48 ± 4.21 ) μg/L than in group A (20.52 ± 4.26,28.25 ± 6.18,36.08 ± 7.45,30.26 ± 6.02) μg/L ( P ＜ 0.05).Conclusion iNOS,and PAF change significantly after the cerebral ischemia-reperfusion in rats,which may be involved in cerebral ischemia-reperfusion injury mechanism.GBE can significantly alleviate the cerebral injury by regulating the expression of iNOS and PAF,suggesting GBE can effectively protect the ischemic cerebral tissues. Key words: Ginkgo biloba extract; Cerebral ischemia; Reperfusion injury; Inducible nitric oxide synthase
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Effects of ginkgo biloba extract on the expression of bax, GFAP after local cerebral ischemia/reperfusion in rats
Chinese journal of experimental surgery, 2012Co-Authors: Chang-hong Duan, Chong-jun Wang, Chao-yuan ZhouAbstract:Objective To explore the protective action of ginkgo biloba extract (GBE) after local cerebral ischemia and reperfusion in rats.Methods 80 male Sprague-Dawley rats were randomly divided into two groups:control group ( group A,saline injected,n =40 ),GBE intervention group ( group GBE injected,n =40),which were divided into 4 subgroups,namely,reperfusion after 1,3,6 and 24 h groups,10 in each subgroup.The animal model of right middle cerebral artery occlusion (MCAO) and reperfusion was established; pathological changes in brain morphology were observed by Hematoxylin and Eosin (HE) staining; serum glial fibrillary acidic protein (GFAP) was detected by enzyme linked immunosorbent assay (ELISA) method; changes of bax mRNA expression was detected by reverse transcriptionpolymerase chain reaction (RT-PCR) method.Results (1) Nerve Cell Necrosis,apoptosis were observed,neuronal shape was difficult to identify,and most of the Cell structure disappeared in group A.While,the injury was milder at same time points in group B compared with group A.(2) ELISA results showed that serum GFAP levels increased after ischemia-reperfusion,the levels in group B (275.29 ±112.72,308.72 ± 124.11,372.56 ± 185.62,452.12 ± 145.26) ng/L were significantly lower than those in group A (437.17 ± 152.26,490.27 ± 198.37,583.45 ± 201.42,656.26 ± 256.36) ng/L,respectively( P ＜ 0.05 ).3.RT-PCR results showed that bax mRNA expression in group B (0.256 ± 0.018,0.302 ±0.023,0.417 ±0.034,0.527 ±0.052) was significantly higher than that in group A (0.333 ±0.021,0.452 ±0.037,0.587±0.052,0.726±0.046,respectively) (P＜0.05).Conclusion GFAP and bax change significantly after cerebral ischemia-reperfusion which may involve in cerebral isehemia reperfusion injury mechanism.GBE can significantly reduce the cerebral injury by down regulating the expression of GFAP and bax.GBE is effective on prevention of cerebral ischemia-reperfusion injury. Key words: Ginkgo biloba extract; Cerebral ischemia-reperfusion injury; Apoptosis; bax; Glial fibrillary acidic protein
Chao-yuan Zhou - One of the best experts on this subject based on the ideXlab platform.
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Effects of ginkgo biloba extract on the expression of bax, GFAP after local cerebral ischemia/reperfusion in rats
Chinese journal of experimental surgery, 2012Co-Authors: Chang-hong Duan, Chong-jun Wang, Chao-yuan ZhouAbstract:Objective To explore the protective action of ginkgo biloba extract (GBE) after local cerebral ischemia and reperfusion in rats.Methods 80 male Sprague-Dawley rats were randomly divided into two groups:control group ( group A,saline injected,n =40 ),GBE intervention group ( group GBE injected,n =40),which were divided into 4 subgroups,namely,reperfusion after 1,3,6 and 24 h groups,10 in each subgroup.The animal model of right middle cerebral artery occlusion (MCAO) and reperfusion was established; pathological changes in brain morphology were observed by Hematoxylin and Eosin (HE) staining; serum glial fibrillary acidic protein (GFAP) was detected by enzyme linked immunosorbent assay (ELISA) method; changes of bax mRNA expression was detected by reverse transcriptionpolymerase chain reaction (RT-PCR) method.Results (1) Nerve Cell Necrosis,apoptosis were observed,neuronal shape was difficult to identify,and most of the Cell structure disappeared in group A.While,the injury was milder at same time points in group B compared with group A.(2) ELISA results showed that serum GFAP levels increased after ischemia-reperfusion,the levels in group B (275.29 ±112.72,308.72 ± 124.11,372.56 ± 185.62,452.12 ± 145.26) ng/L were significantly lower than those in group A (437.17 ± 152.26,490.27 ± 198.37,583.45 ± 201.42,656.26 ± 256.36) ng/L,respectively( P ＜ 0.05 ).3.RT-PCR results showed that bax mRNA expression in group B (0.256 ± 0.018,0.302 ±0.023,0.417 ±0.034,0.527 ±0.052) was significantly higher than that in group A (0.333 ±0.021,0.452 ±0.037,0.587±0.052,0.726±0.046,respectively) (P＜0.05).Conclusion GFAP and bax change significantly after cerebral ischemia-reperfusion which may involve in cerebral isehemia reperfusion injury mechanism.GBE can significantly reduce the cerebral injury by down regulating the expression of GFAP and bax.GBE is effective on prevention of cerebral ischemia-reperfusion injury. Key words: Ginkgo biloba extract; Cerebral ischemia-reperfusion injury; Apoptosis; bax; Glial fibrillary acidic protein
Fu Qing-li - One of the best experts on this subject based on the ideXlab platform.
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Effects of ginkgo biloba extract on the expression of inducible nitric oxide synthase and B Cell lymphoma / lewkmia-2 associated X protein after focal cerebral ischemiareperfusion in rats
2011Co-Authors: Fu Qing-liAbstract:Objective To explore the protective effect and mechanism of extract of ginkgo biloba 761( EGb761) on brain tissue after focal cerebral ischemia-reperfusion injury in rats. Methods Eighty male Sprague Dawley rats were randomly divided into two groups: control group( group A,normal saline injected,n = 40),EGb intervention group( group B, EGb761 injected,n = 40),which were then divided into 4 subgrouops,namely,reperfusion after 1 h,3 h,6 h and 24 h groups,10 in each subgroup. The model of right middle cerebral artery occlusion( MCAO) and reperfusion of rats was made. Pathological changes in brain morphology were observed by HE staining; immunohitochemical method was used to detect the inducible nitric oxide synthase( iNOS) changes; changes of B Cell lymphoma / lewkmia-2 associated X protein( Bax) mRNA expression was detected by reverse transcription-polymerase chain reaction( RT-PCR) method. All data were analyzad by SPSS 13. 0 software. Results( 1) Nerve Cell Necrosis,apoptosis and cytoplasmic vacuolatin were observed in group A,neuronal shape was difficult to identify,and most of the Cell structure disappeared. While,the injury was relieved at the same time points in group B compared with that in group A.( 2) Immunohitochemical results showed that: there were a few iNOS expression positive Cells 1 hour after reperfusion,which increased at 3 hours after referfusion and appeared nucler translocation. Analysis by Image-Pro Plus 5. 0 showed that iNOS expression levels in group B( 1851. 38 ± 242. 12,2005. 41 ± 290. 52,2625. 28 ± 385. 21, 3142. 5 ± 425. 72) were significantly lower than that in group A( 1950. 25 ± 298. 26, 2232. 45 ± 305. 28,3251. 22 ± 425. 26,3965. 36 ± 521. 62,P 0. 05). 3. RT-PCR results showed that bax mRNA expression in group B( 0. 26 ± 0. 02,0. 30 ± 0. 02,0. 42 ± 0. 03, 0. 53 ± 0. 05) was significantly higher than that in group A( 0. 33 ± 0. 02,0. 45 ± 0. 04, 0. 59 ± 0. 05,0. 73 ± 0. 05,P 0. 01 respectively). Conclusions iNOS and Bax increase significantly in rats after cerebral ischemia-reperfusion injury. EGb761 can significantly reduce the cerebral injury by regulating the expression of iNOS and Bax and effectively protect the ischemic cerebral tissue.
